BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00023355
         (1457 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003550988.1| PREDICTED: aspartic proteinase-like protein ...   450   e-124
ref|XP_003525843.1| PREDICTED: aspartic proteinase-like protein ...   445   e-122
ref|XP_002306494.1| predicted protein [Populus trichocarpa] gi|2...   441   e-121
ref|XP_002283258.1| PREDICTED: aspartic proteinase-like protein ...   440   e-121
ref|XP_003516613.1| PREDICTED: aspartic proteinase nepenthesin-2...   423   e-116

>ref|XP_003550988.1| PREDICTED: aspartic proteinase-like protein 2-like [Glycine max]
          Length = 634

 Score =  450 bits (1157), Expect = e-124
 Identities = 217/347 (62%), Positives = 261/347 (75%)
 Frame = -1

Query: 1457 FDGKHGTVLDSGTTYAYLPEAAFLAFKEAIIKELHSLKKIPGPDPNYNDICFSGAGSDVA 1278
            FDGKHGTVLDSGTTYAYLPEAAFLAFK+AI+KEL SLKKI GPDPNYNDICFSGAG DV+
Sbjct: 289  FDGKHGTVLDSGTTYAYLPEAAFLAFKDAIVKELQSLKKISGPDPNYNDICFSGAGIDVS 348

Query: 1277 ELSKAFPTVDMVFENRQKLSLSPENYLFRHSKVRGAYCLGIFQNGKDPTTLLGGIVVRNT 1098
            +LSK+FP VDMVFEN QK +LSPENY+FRHSKVRGAYCLG+FQNG D TTLLGGI+VRNT
Sbjct: 349  QLSKSFPVVDMVFENGQKYTLSPENYMFRHSKVRGAYCLGVFQNGNDQTTLLGGIIVRNT 408

Query: 1097 FVTYDREHEKIGFWKTNCSELWKRLNVTDIHPPEPSAGHRPNSTAIVPPAMAPKGPPHDA 918
             V YDRE  KIGFWKTNC+ELW+RL ++   PP P      NS+  + P++AP    H+A
Sbjct: 409  LVVYDREQTKIGFWKTNCAELWERLQISVAPPPLPPNSGVRNSSEALEPSVAPSVSQHNA 468

Query: 917  RPGEQKVGRITFYMSINVTYSNLKPHIKELTRLMSKELDINVSQVHLINFTSEGNNSLIR 738
            RPGE K+ +IT  +S N++Y ++KPHIKEL  L +  L++N SQVHL+NFTS GN+SL +
Sbjct: 469  RPGELKIVQITMVISFNISYVDMKPHIKELAGLFAHGLNVNTSQVHLLNFTSTGNDSLSK 528

Query: 737  WAILPAGSSSYFSNTTAMIIISKLSEHRVNLPKTFGSYQFSGWSAEPPTKWTWLQHHYXX 558
            WAI P   S Y SNTTAM II++L+EHR+ LP TFG+Y+   WS EPP+K  W Q H+  
Sbjct: 529  WAITPKPDSHYISNTTAMNIIARLAEHRIQLPGTFGNYKLIDWSVEPPSK-NWWQQHFLV 587

Query: 557  XXXXXXXXXXXXXLGFATWFIWRRRQQTLIAYKPVGASVPEQELQPL 417
                             T+ IW++RQQ+  +YKPV   VPEQELQPL
Sbjct: 588  VSLAILITLLLGLSILGTFLIWKKRQQSSHSYKPVDVVVPEQELQPL 634


>ref|XP_003525843.1| PREDICTED: aspartic proteinase-like protein 1-like [Glycine max]
          Length = 663

 Score =  445 bits (1144), Expect = e-122
 Identities = 215/347 (61%), Positives = 257/347 (74%)
 Frame = -1

Query: 1457 FDGKHGTVLDSGTTYAYLPEAAFLAFKEAIIKELHSLKKIPGPDPNYNDICFSGAGSDVA 1278
            FDGKHGTVLDSGTTYAYLPEAAFLAFK+AI+KEL SLK+I GPDPNYNDICFSGAG+DV+
Sbjct: 317  FDGKHGTVLDSGTTYAYLPEAAFLAFKDAIVKELQSLKQISGPDPNYNDICFSGAGNDVS 376

Query: 1277 ELSKAFPTVDMVFENRQKLSLSPENYLFRHSKVRGAYCLGIFQNGKDPTTLLGGIVVRNT 1098
            +LSK+FP VDMVF N  K SLSPENY+FRHSKVRGAYCLGIFQNG D TTLLGGI+VRNT
Sbjct: 377  QLSKSFPVVDMVFGNGHKYSLSPENYMFRHSKVRGAYCLGIFQNGNDQTTLLGGIIVRNT 436

Query: 1097 FVTYDREHEKIGFWKTNCSELWKRLNVTDIHPPEPSAGHRPNSTAIVPPAMAPKGPPHDA 918
             V YDRE  KIGFWKTNC+ELW+RL  +   PP P      NS+  + P++AP    H+A
Sbjct: 437  LVMYDREQTKIGFWKTNCAELWERLQTSIAPPPLPPNSGVRNSSEALEPSVAPSVSQHNA 496

Query: 917  RPGEQKVGRITFYMSINVTYSNLKPHIKELTRLMSKELDINVSQVHLINFTSEGNNSLIR 738
             PGE K+ +IT  +S N++Y ++KPHI EL  L +  LD N SQVHL+NFTS GN+SL +
Sbjct: 497  SPGELKIAQITMVISFNISYVDMKPHITELAGLFAHGLDTNTSQVHLLNFTSTGNDSLSK 556

Query: 737  WAILPAGSSSYFSNTTAMIIISKLSEHRVNLPKTFGSYQFSGWSAEPPTKWTWLQHHYXX 558
            WAI P   + Y SNTTAM II +L+EHR+ LP TFG+Y+   WS EPP+K  W QH +  
Sbjct: 557  WAITPKPYAHYISNTTAMNIIDRLAEHRIQLPSTFGNYKLIDWSVEPPSKNWWQQHFFLV 616

Query: 557  XXXXXXXXXXXXXLGFATWFIWRRRQQTLIAYKPVGASVPEQELQPL 417
                             T+ IW++RQQ+  +YKPV A+VPEQELQPL
Sbjct: 617  VSLAILITLLLGLSILGTFLIWKKRQQSSHSYKPVDAAVPEQELQPL 663


>ref|XP_002306494.1| predicted protein [Populus trichocarpa] gi|222855943|gb|EEE93490.1|
            predicted protein [Populus trichocarpa]
          Length = 564

 Score =  441 bits (1133), Expect = e-121
 Identities = 212/347 (61%), Positives = 256/347 (73%)
 Frame = -1

Query: 1457 FDGKHGTVLDSGTTYAYLPEAAFLAFKEAIIKELHSLKKIPGPDPNYNDICFSGAGSDVA 1278
            FDGKHGT+LDSGTTYAYLPEAAF++FK+AI+KELHSLK I GPDPNYNDICFSGAGSD++
Sbjct: 218  FDGKHGTILDSGTTYAYLPEAAFVSFKDAIMKELHSLKPIRGPDPNYNDICFSGAGSDIS 277

Query: 1277 ELSKAFPTVDMVFENRQKLSLSPENYLFRHSKVRGAYCLGIFQNGKDPTTLLGGIVVRNT 1098
            +LS +FP V+MVF N QKL LSPENYLFRHSKV GAYCLGIFQNGKDPTTLLGGIVVRNT
Sbjct: 278  QLSSSFPAVEMVFGNGQKLLLSPENYLFRHSKVHGAYCLGIFQNGKDPTTLLGGIVVRNT 337

Query: 1097 FVTYDREHEKIGFWKTNCSELWKRLNVTDIHPPEPSAGHRPNSTAIVPPAMAPKGPPHDA 918
             V YDRE+ KIGFWKTNCSELW+RLNV    PP PS+ +  NS   +PP++AP    H  
Sbjct: 338  LVLYDRENSKIGFWKTNCSELWERLNVDGAPPPAPSSSNGNNSNTEMPPSVAPSDQKHYG 397

Query: 917  RPGEQKVGRITFYMSINVTYSNLKPHIKELTRLMSKELDINVSQVHLINFTSEGNNSLIR 738
             P E+K+G+ITF M +NV YS+LK HI EL   +++EL IN SQV+++N   +GN S I 
Sbjct: 398  LPDEKKIGQITFEMMLNVNYSDLKLHISELAESIAQELGINSSQVYILNSMEKGNASYIE 457

Query: 737  WAILPAGSSSYFSNTTAMIIISKLSEHRVNLPKTFGSYQFSGWSAEPPTKWTWLQHHYXX 558
            WA++P+GS+   SN TA+ II++++E+ ++LP TFGSY    W  +   K TW Q H+  
Sbjct: 458  WAVVPSGSADCISNVTALSIIARVAEYHLHLPDTFGSYHLINWEIKASAKRTWWQQHFLL 517

Query: 557  XXXXXXXXXXXXXLGFATWFIWRRRQQTLIAYKPVGASVPEQELQPL 417
                         L    WFIWR RQ+ L  YKPV A V EQELQPL
Sbjct: 518  VVLASAVTFIFGLLALGIWFIWRHRQRALNPYKPVDAVVTEQELQPL 564


>ref|XP_002283258.1| PREDICTED: aspartic proteinase-like protein 2 [Vitis vinifera]
            gi|302142232|emb|CBI19435.3| unnamed protein product
            [Vitis vinifera]
          Length = 659

 Score =  440 bits (1132), Expect = e-121
 Identities = 215/360 (59%), Positives = 260/360 (72%), Gaps = 13/360 (3%)
 Frame = -1

Query: 1457 FDGKHGTVLDSGTTYAYLPEAAFLAFKEAIIKELHSLKKIPGPDPNYNDICFSGAGSDVA 1278
            FD KHGTVLDSGTTYAYLPE AF+AF++AIIK+ H+LK+I GPDPNYNDICFSGAG DV+
Sbjct: 293  FDRKHGTVLDSGTTYAYLPEEAFVAFRDAIIKKSHNLKQIHGPDPNYNDICFSGAGRDVS 352

Query: 1277 ELSKAFPTVDMVFENRQKLSLSPENYLFRHSKVRGAYCLGIFQNGKDPTTLLGGIVVRNT 1098
            +LSKAFP VDMVF N QKLSL+PENYLF+H+KV GAYCLGIF+NG D TTLLGGI+VRNT
Sbjct: 353  QLSKAFPEVDMVFSNGQKLSLTPENYLFQHTKVHGAYCLGIFRNG-DSTTLLGGIIVRNT 411

Query: 1097 FVTYDREHEKIGFWKTNCSELWKRLNV-------------TDIHPPEPSAGHRPNSTAIV 957
             VTYDRE+EKIGFWKTNCSELWKRL++               +  P P   +  N+T  +
Sbjct: 412  LVTYDRENEKIGFWKTNCSELWKRLHIPGAPAAAPIVPTPKSVSAPAPVVSYNNNTTVGM 471

Query: 956  PPAMAPKGPPHDARPGEQKVGRITFYMSINVTYSNLKPHIKELTRLMSKELDINVSQVHL 777
            PP +AP G P +  PGE +VG ITF MS +V YSN+KP+  EL   ++ EL+IN SQVH 
Sbjct: 472  PPTVAPSGLPQEVLPGEFQVGLITFDMSFSVNYSNMKPNFTELAEFIAHELEINASQVHF 531

Query: 776  INFTSEGNNSLIRWAILPAGSSSYFSNTTAMIIISKLSEHRVNLPKTFGSYQFSGWSAEP 597
            +NF S+GN+S+IRWAI PA S++Y SN+TAM II +L EHRV+LP+ FGSYQ   W  EP
Sbjct: 532  LNFFSKGNHSVIRWAIFPAESATYISNSTAMSIILQLKEHRVHLPERFGSYQLVEWKVEP 591

Query: 596  PTKWTWLQHHYXXXXXXXXXXXXXXXLGFATWFIWRRRQQTLIAYKPVGASVPEQELQPL 417
              K TW + H+                 F  WF+W+ RQ  +  YKP+GA VPEQELQ L
Sbjct: 592  QIKRTWWEQHFWTVVVGVIITLILGLSTFGVWFVWKWRQNAVGTYKPIGARVPEQELQQL 651


>ref|XP_003516613.1| PREDICTED: aspartic proteinase nepenthesin-2-like [Glycine max]
          Length = 645

 Score =  423 bits (1088), Expect = e-116
 Identities = 206/348 (59%), Positives = 253/348 (72%), Gaps = 1/348 (0%)
 Frame = -1

Query: 1457 FDGKHGTVLDSGTTYAYLPEAAFLAFKEAIIKELHSLKKIPGPDPNYNDICFSGAGSDVA 1278
            FDGKHGTVLDSGTTYAYLPE+AFLAFK AI+KE HSLK+I GPDP YNDICFSGA  DV+
Sbjct: 298  FDGKHGTVLDSGTTYAYLPESAFLAFKHAIMKETHSLKRISGPDPRYNDICFSGAEIDVS 357

Query: 1277 ELSKAFPTVDMVFENRQKLSLSPENYLFRHSKVRGAYCLGIFQNGKDPTTLLGGIVVRNT 1098
            ++SK+FP V+MVF N  KLSLSPENYLFRHSKVRGAYCLG+F NG DPTTLLGGIVVRNT
Sbjct: 358  QISKSFPVVEMVFGNGHKLSLSPENYLFRHSKVRGAYCLGVFSNGNDPTTLLGGIVVRNT 417

Query: 1097 FVTYDREHEKIGFWKTNCSELWKRLNVTDIHPP-EPSAGHRPNSTAIVPPAMAPKGPPHD 921
             V YDREH KIGFWKTNCSELW+RL+V+D  PP  P      N T    P++AP    ++
Sbjct: 418  LVMYDREHTKIGFWKTNCSELWERLHVSDAPPPLLPPKSEGTNLTKSFEPSIAPSPSQYN 477

Query: 920  ARPGEQKVGRITFYMSINVTYSNLKPHIKELTRLMSKELDINVSQVHLINFTSEGNNSLI 741
             + GE ++ +I   +S N++Y ++KP+I ELT L++ ELD+N SQVHL+NF+S GN SL 
Sbjct: 478  LQLGELQIAQIIVVISFNISYMDMKPYITELTGLIAHELDVNSSQVHLMNFSSLGNGSLS 537

Query: 740  RWAILPAGSSSYFSNTTAMIIISKLSEHRVNLPKTFGSYQFSGWSAEPPTKWTWLQHHYX 561
            +W I P   + +FSN TAM +I++LSEHR+ LP + GSY+   W+AEPP K TW Q +Y 
Sbjct: 538  KWVITPRPYADFFSNATAMSMIARLSEHRMQLPNSVGSYKLVDWNAEPPLKRTWWQQYYL 597

Query: 560  XXXXXXXXXXXXXXLGFATWFIWRRRQQTLIAYKPVGASVPEQELQPL 417
                               + IW++RQQ   +YKPV  +V EQELQPL
Sbjct: 598  VVALAVLLTFVLGISTLGIFLIWKKRQQAEHSYKPVDVAVQEQELQPL 645