BLASTX nr result
ID: Bupleurum21_contig00023332
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00023332
(1449 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
dbj|BAB68539.1| (S)-reticuline oxidase-like protein [Daucus carota] 451 e-124
ref|XP_002332196.1| predicted protein [Populus trichocarpa] gi|2... 441 e-121
ref|XP_003638113.1| Reticuline oxidase [Medicago truncatula] gi|... 440 e-121
ref|XP_002523148.1| Reticuline oxidase precursor, putative [Rici... 432 e-118
ref|XP_002333339.1| predicted protein [Populus trichocarpa] gi|2... 429 e-118
>dbj|BAB68539.1| (S)-reticuline oxidase-like protein [Daucus carota]
Length = 506
Score = 451 bits (1160), Expect = e-124
Identities = 230/432 (53%), Positives = 301/432 (69%), Gaps = 4/432 (0%)
Frame = -2
Query: 1316 TSSSSHEHFVQCLTRFSPNSNSIIKVIYTPQNSSYTSVLEFSINNLRFSTPSTPKPLVIV 1137
TS+ S + F+QCLT+++ NS SI +V++TP N+SY +L+ ++ NLRF+T T KPL IV
Sbjct: 1 TSADSTQRFIQCLTKYAKNSESISQVVFTPANASYNPILQLNLQNLRFNTSGTRKPLAIV 60
Query: 1136 TPKQDSQIQTVIYCSKKHDIQMRIRGGGHDYEGLSYVSKVPFVLLDMNKYRSVNVDHVAA 957
TP +++QIQTVIYC++K+ + +R RGGGHD+EG+SY ++VPFVLLDM + VN+D +
Sbjct: 61 TPIEETQIQTVIYCARKNSMNVRTRGGGHDFEGVSYTAEVPFVLLDMINFNRVNIDLKTS 120
Query: 956 TAWVQSGATLGELYYHIANKSDTLAFPGGIWYTVGVTGLISGGGYGPLRRKYGLAADNVI 777
TAWVQSG +LGE YY I+ KSD LAFP G+ +VG+TGL+ GGGYG L+RKY LAADN +
Sbjct: 121 TAWVQSGISLGEFYYRISQKSDVLAFPAGLLSSVGLTGLLGGGGYGMLKRKYALAADNTL 180
Query: 776 DARLIDANGRILDRKSMGEDLFWAIRGGGASSFGVILSWKLKLVQVPKIVTGFQADRTLE 597
DAR++D NG+ILDRKSMGEDLFWAIRGG +SF V+L KL+LV VPK VT F RTLE
Sbjct: 181 DARIVDYNGKILDRKSMGEDLFWAIRGGDPASFCVVLELKLQLVPVPKSVTYFAVQRTLE 240
Query: 596 QNGSELVHKWQTIAPNL-PKNADVRIVTNVVWKNKTNGPIRTILSTADQKSDEKTVRFRF 420
QNGS L KWQ A N+ P++ DVR+V + TI S + + D+KTVRF F
Sbjct: 241 QNGSALFQKWQATAANVFPRDLDVRVV------------VDTITSNSSPRQDKKTVRFVF 288
Query: 419 LGSYLGSRDEFISLMNKKFPALGLVAEECVEVSYMQLELLFSLFQATDSPTKLLSRKSYT 240
YLG D + +M K FP LGLV ++C E S+++ +FS F PT LL++ +
Sbjct: 289 QCLYLGKIDTLLPIMQKYFPELGLVRDDCTETSWIKTAPMFSGFPVGTDPTILLNKTA-- 346
Query: 239 IP---FKSKSSFVNKPISIRGLNGIWKLLLKQDPMMINFILTSYGGRMSEISESAIPFPH 69
IP K KSSF +PIS+ GLNGIW L LKQ T +GG M+E +ESA+PFPH
Sbjct: 347 IPRNSVKIKSSFTTQPISLEGLNGIWDLWLKQPVQTTLIQYTPFGGIMNEFAESALPFPH 406
Query: 68 RAGTLYMMYMRI 33
R G LYM+ M +
Sbjct: 407 RPGVLYMINMAV 418
>ref|XP_002332196.1| predicted protein [Populus trichocarpa] gi|222875303|gb|EEF12434.1|
predicted protein [Populus trichocarpa]
Length = 527
Score = 441 bits (1135), Expect = e-121
Identities = 236/437 (54%), Positives = 299/437 (68%), Gaps = 3/437 (0%)
Frame = -2
Query: 1352 LLVLFFL--NISLVTSSSSHEHFVQCLTRFSPNSNSIIKVIYTPQNSSYTSVLEFSINNL 1179
L LFFL + S VTS+ +HE F+QCL S NS+SI K+IYTP NSSY+S+L+FSI NL
Sbjct: 2 LSFLFFLIFSFSWVTSAHTHESFLQCLD--SQNSHSISKLIYTPINSSYSSILQFSIQNL 59
Query: 1178 RFSTPSTPKPLVIVTPKQDSQIQTVIYCSKKHDIQMRIRGGGHDYEGLSYVSKVPFVLLD 999
RF+T STPKPLVIVTP S IQ I CS+KH +Q+RIR GGHDYEGLSYVS FV++D
Sbjct: 60 RFNTSSTPKPLVIVTPTNISHIQAAIICSQKHGMQIRIRSGGHDYEGLSYVSTFSFVVID 119
Query: 998 MNKYRSVNVDHVAATAWVQSGATLGELYYHIANKSDTLAFPGGIWYTVGVTGLISGGGYG 819
+ R++NV+ TAWVQSG T+GE+YY IA KS TLAFP + TVGV G SGGGYG
Sbjct: 120 LINLRTINVNAENKTAWVQSGVTIGEVYYRIAEKSRTLAFPASVCPTVGVGGHFSGGGYG 179
Query: 818 PLRRKYGLAADNVIDARLIDANGRILDRKSMGEDLFWAIRGGGASSFGVILSWKLKLVQV 639
L RKYGLAAD++IDA+L+D GRILDR+SMGEDLFWAIRGGG ++FGV+++WK+ LV
Sbjct: 180 MLMRKYGLAADHIIDAQLVDVKGRILDRESMGEDLFWAIRGGGGNTFGVVIAWKISLVSA 239
Query: 638 PKIVTGFQADRTLEQNGSELVHKWQTIAPNLPKNADVRIVTNVVWKNKTNGPIRTILSTA 459
P VT F RTLEQN S+LVH+WQ +A L ++ +RI+ N + ST
Sbjct: 240 PPTVTVFNVQRTLEQNASKLVHRWQFVADKLHEDLFIRIILN------------RVNSTE 287
Query: 458 DQKSDEKTVRFRFLGSYLGSRDEFISLMNKKFPALGLVAEECVEVSYMQLELLFSLFQAT 279
+ KS+ T++ F +LG D + L+ FP LGLV E+C+E+S++Q L F F +
Sbjct: 288 EGKSN-TTIQASFNSLFLGGIDRLLPLIQDSFPELGLVKEDCIEMSWIQSVLYFDGFPSN 346
Query: 278 DSPTKLLSRKSYT-IPFKSKSSFVNKPISIRGLNGIWKLLLKQDPMMINFILTSYGGRMS 102
S LL R T FK+KS +V +PI GL GIW+ +D I + YGG+MS
Sbjct: 347 SSLDVLLDRTPSTRRNFKAKSDYVKEPIPELGLEGIWERFFDKDINTPILIFSPYGGKMS 406
Query: 101 EISESAIPFPHRAGTLY 51
EISES+IPFPHRAG +Y
Sbjct: 407 EISESSIPFPHRAGNIY 423
>ref|XP_003638113.1| Reticuline oxidase [Medicago truncatula] gi|355504048|gb|AES85251.1|
Reticuline oxidase [Medicago truncatula]
Length = 543
Score = 440 bits (1132), Expect = e-121
Identities = 236/441 (53%), Positives = 293/441 (66%), Gaps = 2/441 (0%)
Frame = -2
Query: 1367 YQCSYLLVLFFLNISLVTSSSSHE-HFVQCLTRFSPNSNSIIKVIYTPQNSSYTSVLEFS 1191
Y L+ + F S +S HE +F+QCL +S N SI KV+YT NSSY+S+L+FS
Sbjct: 7 YLTIVLIAIAFSFTSFAIDTSPHEDNFLQCLYSYSHNITSISKVVYTKTNSSYSSILKFS 66
Query: 1190 INNLRFSTPSTPKPLVIVTPKQDSQIQTVIYCSKKHDIQMRIRGGGHDYEGLSYVSKVPF 1011
I NLRF+T TPKPLVI+TP Q S IQT I CS+ H +Q+RIR GGHD+EGLS+VS VPF
Sbjct: 67 IQNLRFATNETPKPLVIITPTQISHIQTAIICSQHHGMQIRIRSGGHDFEGLSFVSNVPF 126
Query: 1010 VLLDMNKYRSVNVDHVAATAWVQSGATLGELYYHIANKSDTLAFPGGIWYTVGVTGLISG 831
V++D+ +R ++VD TAWVQSGATLGELYY IA KS TL FPGG+ TVGV G SG
Sbjct: 127 VIIDLTNFRGIDVDVENRTAWVQSGATLGELYYKIAQKSKTLGFPGGVCPTVGVGGHFSG 186
Query: 830 GGYGPLRRKYGLAADNVIDARLIDANGRILDRKSMGEDLFWAIRGGGASSFGVILSWKLK 651
GGYG L RKYGLAADNVIDA +ID GR LDR++MGEDLFWAIRGGG +SFGVI+SWK+K
Sbjct: 187 GGYGTLLRKYGLAADNVIDAHIIDVKGRFLDREAMGEDLFWAIRGGGGASFGVIVSWKIK 246
Query: 650 LVQVPKIVTGFQADRTLEQNGSELVHKWQTIAPNLPKNADVRIVTNVVWKNKTNGPIRTI 471
LVQVP VT F RTLEQN ++LVHKWQ +A L +N + I+ R
Sbjct: 247 LVQVPSTVTVFTVPRTLEQNATKLVHKWQFVAHKLEENLAINIILQ-----------RLD 295
Query: 470 LSTADQKSDEKTVRFRFLGSYLGSRDEFISLMNKKFPALGLVAEECVEVSYMQLELLFSL 291
L+++ Q + TV F +LGS D + LM +KFP LGLV E+CVE+S+++ L
Sbjct: 296 LNSSKQGEPKSTVLALFQSLFLGSVDNLLPLMEEKFPELGLVREDCVEMSWIESVLYLFR 355
Query: 290 FQATDSPTKLLSRK-SYTIPFKSKSSFVNKPISIRGLNGIWKLLLKQDPMMINFILTSYG 114
F + LL+R + K+KS FV PI GL G+W L + + +L YG
Sbjct: 356 FPEGEPLETLLNRTLAAKDNSKAKSDFVKIPIPETGLEGLWPLFDEDGAEDVLMVLFPYG 415
Query: 113 GRMSEISESAIPFPHRAGTLY 51
G M +ISES IPFPHR GTLY
Sbjct: 416 GIMDKISESEIPFPHRYGTLY 436
>ref|XP_002523148.1| Reticuline oxidase precursor, putative [Ricinus communis]
gi|223537555|gb|EEF39179.1| Reticuline oxidase precursor,
putative [Ricinus communis]
Length = 442
Score = 432 bits (1110), Expect = e-118
Identities = 225/436 (51%), Positives = 299/436 (68%), Gaps = 2/436 (0%)
Frame = -2
Query: 1352 LLVLFFLNISLVTSSSSHEHFVQCLTRFSPNSNSIIKVIYTPQNSSYTSVLEFSINNLRF 1173
+LV L+ S TS+ +HE F++CL S +S SI ++IYTP NSSY+SVL+FSI N RF
Sbjct: 10 VLVFSLLSFSWATSAQTHEDFLECLHLQSQDSTSISRIIYTPINSSYSSVLQFSIQNRRF 69
Query: 1172 STPSTPKPLVIVTPKQDSQIQTVIYCSKKHDIQMRIRGGGHDYEGLSYVSKVPFVLLDMN 993
+T +TPKPLVIVTP S +Q I CSK+H + +R+R GGHDYEGLSYVS PFV++D+
Sbjct: 70 NTSTTPKPLVIVTPLNVSHVQAAIICSKRHGMHIRVRSGGHDYEGLSYVSVHPFVIVDLI 129
Query: 992 KYRSVNVDHVAATAWVQSGATLGELYYHIANKSDTLAFPGGIWYTVGVTGLISGGGYGPL 813
+S+ VD TAWVQ+GAT+G LYY IA +S TLAFP G+ TVG+ G +GGGYG L
Sbjct: 130 NLQSITVDAANNTAWVQAGATIGNLYYSIAERSRTLAFPAGVCPTVGIGGHFTGGGYGML 189
Query: 812 RRKYGLAADNVIDARLIDANGRILDRKSMGEDLFWAIRGGGASSFGVILSWKLKLVQVPK 633
RKYGLAADN+IDA LID NGR+LDR SMGEDLFWAIRGGG ++FG+++SWK+ LV VP
Sbjct: 190 LRKYGLAADNIIDAVLIDVNGRVLDRASMGEDLFWAIRGGGGNTFGIVISWKINLVPVPA 249
Query: 632 IVTGFQADRTLEQNGSELVHKWQTIAPNLPKNADVRIVTNVVWKNKTNGPIRTILSTADQ 453
VT F ++TLEQN ++LV++WQ IA L ++ +R++ I + ST+ Q
Sbjct: 250 TVTVFTVEKTLEQNATQLVNRWQYIADKLHEDLFIRVI------------IERVNSTSQQ 297
Query: 452 KSDEKTVRFRFLGSYLGSRDEFISLMNKKFPALGLVAEECVEVSYMQLELLFSLFQATDS 273
+ TVR F +LG D + LM + FP LGLV E+C+E+S+++ L F+ F ++S
Sbjct: 298 --GKTTVRAAFNSLFLGGVDRLLPLMQESFPELGLVREDCIEMSWIESILYFAGF--SNS 353
Query: 272 PTKLLSRKSY--TIPFKSKSSFVNKPISIRGLNGIWKLLLKQDPMMINFILTSYGGRMSE 99
P +L ++ FK+KS +V +P+ L GIW+ L + D I + YGGRMSE
Sbjct: 354 PLDILLNRTQPSVRNFKAKSDYVKEPMPETALEGIWERLSEVDVGAGQLIFSPYGGRMSE 413
Query: 98 ISESAIPFPHRAGTLY 51
ISES+IPFPHRAG LY
Sbjct: 414 ISESSIPFPHRAGNLY 429
>ref|XP_002333339.1| predicted protein [Populus trichocarpa] gi|222836259|gb|EEE74680.1|
predicted protein [Populus trichocarpa]
Length = 533
Score = 429 bits (1104), Expect = e-118
Identities = 222/437 (50%), Positives = 299/437 (68%), Gaps = 2/437 (0%)
Frame = -2
Query: 1349 LVLFFLNISLVTSSSSHEHFVQCLTRFSPNSNSIIKVIYTPQNSSYTSVLEFSINNLRFS 1170
L+ ++S VTS+ HE F+QCL+ S +S +I KVIYTP+NSSY+S+L FSI N RF+
Sbjct: 12 LICLLFSLSCVTSARPHEDFLQCLSLHSEDSTAISKVIYTPKNSSYSSILHFSIRNPRFN 71
Query: 1169 TPSTPKPLVIVTPKQDSQIQTVIYCSKKHDIQMRIRGGGHDYEGLSYVSKVPFVLLDMNK 990
+ S KP VIVTP S IQ I+CS+KH +++RIR GGHD+EGLSY+S VPFV++D+
Sbjct: 72 S-SELKPFVIVTPTDASHIQAAIHCSQKHKLEIRIRSGGHDFEGLSYMSTVPFVIVDLIN 130
Query: 989 YRSVNVDHVAATAWVQSGATLGELYYHIANKSDTLAFPGGIWYTVGVTGLISGGGYGPLR 810
RS+ VD TAWVQ+GAT+GELYY IA KS TLAFP G TVGV G SGGGYG +
Sbjct: 131 LRSITVDATNKTAWVQAGATIGELYYRIAEKSRTLAFPAGSCPTVGVGGHFSGGGYGTIS 190
Query: 809 RKYGLAADNVIDARLIDANGRILDRKSMGEDLFWAIRGGGASSFGVILSWKLKLVQVPKI 630
RKYGLA+DNVIDA+LIDA GRILDR+SMGEDLFWAIRGGG SFGV+++W++KLV+VP
Sbjct: 191 RKYGLASDNVIDAQLIDAKGRILDRESMGEDLFWAIRGGGGQSFGVVIAWRIKLVEVPPK 250
Query: 629 VTGFQADRTLEQNGSELVHKWQTIAPNLPKNADVRIVTNVVWKNKTNGPIRTILSTADQK 450
VT F A RTLEQN ++L+H+WQ +A LP++ + ++ N + S+ + K
Sbjct: 251 VTVFIAARTLEQNATKLIHRWQYVANQLPEDIIIDVLVN------------RVNSSEEGK 298
Query: 449 SDEKTVRFRFLGSYLGSRDEFISLMNKKFPALGLVAEECVEVSYMQLELLFSLFQATDSP 270
S T++ F +LG D+ + LM + FP LGL +EC E+S+++ + F + S
Sbjct: 299 S---TIQAAFFSLFLGEVDQLLLLMQESFPELGLAKDECTEMSWIESVIYIGGFPSNASL 355
Query: 269 TKLLSR--KSYTIPFKSKSSFVNKPISIRGLNGIWKLLLKQDPMMINFILTSYGGRMSEI 96
LL R + ++ FK+KS +V +PI GIWK ++D + F + +YGG+M EI
Sbjct: 356 NVLLDRTPQPPSLQFKAKSDYVQEPIPEIAFEGIWKRFFEKDIEVPVFFMVAYGGKMDEI 415
Query: 95 SESAIPFPHRAGTLYMM 45
SES+ PFPHRAG Y++
Sbjct: 416 SESSTPFPHRAGNRYIV 432