BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00023256
         (376 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAS01523.1| putative beta-amyrin synthase [Centella asiatica]      239   1e-61
gb|AFJ19235.1| mixed amyrin synthase [Catharanthus roseus]            238   4e-61
gb|AEX99665.1| amyrin synthase [Catharanthus roseus]                  238   4e-61
dbj|BAF63702.1| mixed amyrin synthase [Olea europaea]                 234   4e-60
dbj|BAD15332.1| beta-amyrin synthase [Panax ginseng]                  221   6e-56

>gb|AAS01523.1| putative beta-amyrin synthase [Centella asiatica]
          Length = 760

 Score =  239 bits (611), Expect = 1e-61
 Identities = 112/125 (89%), Positives = 117/125 (93%)
 Frame = -1

Query: 376 NRAIQAHDGHWPAENAGPMFFTPPLLIALYISGAMDGVLTQEHKKEMIRFIYNHQNKDGG 197
           NRAIQAHDGHWPAENAGPMFFTPPL+IALYISGA+D  LT +HKKEMIRFIY HQNKDGG
Sbjct: 107 NRAIQAHDGHWPAENAGPMFFTPPLIIALYISGAIDTHLTIQHKKEMIRFIYLHQNKDGG 166

Query: 196 WGFYIEGHSTMIGSALSYVALRLLGEGPNDGGGTVERGRKWILDHGGATSIPSWGKTYLS 17
           WGFYIEGHSTMIGSALSYVALRLLGEGP+DG G VER RKWILDHGGA SIPSWGKTYL+
Sbjct: 167 WGFYIEGHSTMIGSALSYVALRLLGEGPDDGDGAVERARKWILDHGGAASIPSWGKTYLA 226

Query: 16  VLGVY 2
           VLGVY
Sbjct: 227 VLGVY 231


>gb|AFJ19235.1| mixed amyrin synthase [Catharanthus roseus]
          Length = 762

 Score =  238 bits (607), Expect = 4e-61
 Identities = 109/125 (87%), Positives = 118/125 (94%)
 Frame = -1

Query: 376 NRAIQAHDGHWPAENAGPMFFTPPLLIALYISGAMDGVLTQEHKKEMIRFIYNHQNKDGG 197
           NRAIQA DGHWPAENAGPMFFTPPLLIALYISGA++ +LT EHKKE++R+IYNHQN+DGG
Sbjct: 107 NRAIQATDGHWPAENAGPMFFTPPLLIALYISGAINTILTSEHKKELVRYIYNHQNEDGG 166

Query: 196 WGFYIEGHSTMIGSALSYVALRLLGEGPNDGGGTVERGRKWILDHGGATSIPSWGKTYLS 17
           WGFYIEGHSTMIGSALSYVALRLLGEGP+DG G V RGR+WILDHGGAT IPSWGKTYLS
Sbjct: 167 WGFYIEGHSTMIGSALSYVALRLLGEGPDDGDGAVGRGRQWILDHGGATGIPSWGKTYLS 226

Query: 16  VLGVY 2
           VLGVY
Sbjct: 227 VLGVY 231


>gb|AEX99665.1| amyrin synthase [Catharanthus roseus]
          Length = 762

 Score =  238 bits (607), Expect = 4e-61
 Identities = 109/125 (87%), Positives = 118/125 (94%)
 Frame = -1

Query: 376 NRAIQAHDGHWPAENAGPMFFTPPLLIALYISGAMDGVLTQEHKKEMIRFIYNHQNKDGG 197
           NRAIQA DGHWPAENAGPMFFTPPLLIALYISGA++ +LT EHKKE++R+IYNHQN+DGG
Sbjct: 107 NRAIQATDGHWPAENAGPMFFTPPLLIALYISGAINTILTSEHKKELVRYIYNHQNEDGG 166

Query: 196 WGFYIEGHSTMIGSALSYVALRLLGEGPNDGGGTVERGRKWILDHGGATSIPSWGKTYLS 17
           WGFYIEGHSTMIGSALSYVALRLLGEGP+DG G V RGR+WILDHGGAT IPSWGKTYLS
Sbjct: 167 WGFYIEGHSTMIGSALSYVALRLLGEGPDDGDGAVGRGRQWILDHGGATGIPSWGKTYLS 226

Query: 16  VLGVY 2
           VLGVY
Sbjct: 227 VLGVY 231


>dbj|BAF63702.1| mixed amyrin synthase [Olea europaea]
          Length = 762

 Score =  234 bits (598), Expect = 4e-60
 Identities = 104/125 (83%), Positives = 116/125 (92%)
 Frame = -1

Query: 376 NRAIQAHDGHWPAENAGPMFFTPPLLIALYISGAMDGVLTQEHKKEMIRFIYNHQNKDGG 197
           NRA+QA DGHWPAENAGPMFFTPPL+I LYISGA++ +LT EH+KEM+R+IYNHQN DGG
Sbjct: 107 NRAVQASDGHWPAENAGPMFFTPPLIIVLYISGAINTILTSEHRKEMVRYIYNHQNDDGG 166

Query: 196 WGFYIEGHSTMIGSALSYVALRLLGEGPNDGGGTVERGRKWILDHGGATSIPSWGKTYLS 17
           WGFYIEGHSTMIGSALSY+ALRLLGEGP+DG G++ R RKWILDHGGAT IPSWGKTYLS
Sbjct: 167 WGFYIEGHSTMIGSALSYIALRLLGEGPDDGNGSIARARKWILDHGGATGIPSWGKTYLS 226

Query: 16  VLGVY 2
           VLGVY
Sbjct: 227 VLGVY 231


>dbj|BAD15332.1| beta-amyrin synthase [Panax ginseng]
          Length = 769

 Score =  221 bits (562), Expect = 6e-56
 Identities = 101/128 (78%), Positives = 113/128 (88%), Gaps = 3/128 (2%)
 Frame = -1

Query: 376 NRAIQAHDGHWPAENAGPMFFTPPLLIALYISGAMDGVLTQEHKKEMIRFIYNHQNKDGG 197
           NRAIQAHDGHWPAENAG + +TPPL+IALYISG +D +LT++HKKE+IRF+YNHQN+DGG
Sbjct: 107 NRAIQAHDGHWPAENAGSLLYTPPLIIALYISGTIDTILTKQHKKELIRFVYNHQNEDGG 166

Query: 196 WGFYIEGHSTMIGSALSYVALRLLGEG---PNDGGGTVERGRKWILDHGGATSIPSWGKT 26
           WG YIEGHSTMIGS LSYV LRLLGEG    +DG G VERGRKWILDHGGA  IPSWGKT
Sbjct: 167 WGSYIEGHSTMIGSVLSYVMLRLLGEGLAESDDGNGAVERGRKWILDHGGAAGIPSWGKT 226

Query: 25  YLSVLGVY 2
           YL+VLGVY
Sbjct: 227 YLAVLGVY 234