BLASTX nr result
ID: Bupleurum21_contig00023128
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00023128
(1577 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAC13599.1| similar to reverse transcriptase (Pfam: transcrip... 385 e-104
gb|AAC28221.1| similar to reverse transcriptases (PFam: rvt.hmm,... 374 e-101
dbj|BAB09379.1| non-LTR retroelement reverse transcriptase-like ... 371 e-100
gb|AAC33226.1| putative non-LTR retroelement reverse transcripta... 369 e-100
emb|CCA65981.1| hypothetical protein [Beta vulgaris subsp. vulga... 369 2e-99
>gb|AAC13599.1| similar to reverse transcriptase (Pfam: transcript_fact.hmm, score:
72.31) [Arabidopsis thaliana]
Length = 928
Score = 385 bits (990), Expect = e-104
Identities = 215/527 (40%), Positives = 310/527 (58%), Gaps = 8/527 (1%)
Frame = +3
Query: 21 SWFVLTTKLKRVKQALKCLNNS-IGNVHLAVQEARNELYNLQNCIIGAPSDTQAIEERNL 197
S F + KLK +K L+ L +GN+ +EA L Q + PS + EE
Sbjct: 171 SLFRFSKKLKGLKPLLRNLGKERLGNLVKQTKEAFETLCQKQAMKMANPSPSSMQEENEA 230
Query: 198 MMKYQAALDSEENFLQQKSKVHWLQKGDGNNRFFFNYCRGRWNTNRIVGLQDP*GSLVTD 377
K+ EE FL+Q+SK+HWL GD NN+ F R N I + GS+ +
Sbjct: 231 YAKWDHIAVLEEKFLKQRSKLHWLDIGDRNNKAFHRAVVAREAQNSIREIICHDGSVASQ 290
Query: 378 HHGIANIAVDYYRNLLGTEK------LVEPLPDLNLPSIPDDLGRSLIAPISSTEILRTL 539
I A ++R L VE L DL D L +S+ EI + +
Sbjct: 291 EEKIKTEAEHHFREFLQLIPNDFEGIAVEELQDLLPYRCSDSDKEMLTNHVSAEEIHKVV 350
Query: 540 KSMKKNRSPGPDGFTPDFYIAVWDVVGSDLVNALHSFFDALDLPRQINATAISLIPKVDS 719
SM ++SPGPDG+T +FY W+++G++ + A+ SFF LP+ IN+T ++LIPK
Sbjct: 351 FSMPNDKSPGPDGYTAEFYKGAWNIIGAEFILAIQSFFAKGFLPKGINSTILALIPKKKE 410
Query: 720 PVEMHQFRPISCCNVLYKCITKILANRIKPVLKNLISFNQSAFIPSRSMGDNILLSQALC 899
EM +RPISCCNVLYK I+KI+ANR+K VL I NQSAF+ R + +N+LL+ +
Sbjct: 411 AKEMKDYRPISCCNVLYKVISKIIANRLKLVLPKFIVGNQSAFVKDRLLIENVLLATEIV 470
Query: 900 RSYHLNKGAPRCMLKLDISKAFDSINWSFILNVLNAMHFPAKFTSWIRKCISTCMFSVKI 1079
+ YH + + RC LK+DISKAFDS+ W F++NVL AM+FP +FT WI CI+T FSV++
Sbjct: 471 KDYHKDSVSSRCALKIDISKAFDSVQWKFLINVLEAMNFPPEFTHWITLCITTASFSVQV 530
Query: 1080 NGGLEGFFEGKSGVRQGDPLSPYLFVIAMEVLTCCL-ASVTSADFQFHPKCKDLKLSHLI 1256
NG L G F +RQG LSPYLFVI+M+VL+ L +V + F +HPKC+ + L+HL
Sbjct: 531 NGELAGVFSSARELRQGCSLSPYLFVISMDVLSKMLDKAVGARQFGYHPKCRAIGLTHLS 590
Query: 1257 FADDVLLFSHGDPRSVNTLLKGVDTFSRISGLHLNMQKSLIFFGNVRPADASAILQSSNL 1436
FADD+++ S G RS++ ++K + F++ SGL ++M+KS ++ V+ + I+Q +
Sbjct: 591 FADDLMILSDGKVRSIDGIVKVLYEFAKWSGLKISMEKSTMYLAGVQASVYQEIVQKFSF 650
Query: 1437 QRGSFPFSYLGIPLVTSRINAQLCTPLIMKLCARVNSWTGRFLSFGG 1577
G P YLG+PLV+ R+ A C PLI +L ++ +WT RFLSF G
Sbjct: 651 DVGKLPVRYLGLPLVSKRLTASDCLPLIEQLRKKIEAWTSRFLSFAG 697
>gb|AAC28221.1| similar to reverse transcriptases (PFam: rvt.hmm, score: 60.13)
[Arabidopsis thaliana]
Length = 1164
Score = 374 bits (959), Expect = e-101
Identities = 199/530 (37%), Positives = 315/530 (59%), Gaps = 7/530 (1%)
Frame = +3
Query: 9 VEGDSWFVLTTKLKRVKQALKCLN-NSIGNVHLAVQEARNELYNLQNCIIGAPSDTQAIE 185
V G + + ++ KLK +K+ ++ + ++ ++ +EA + L Q+ ++ +P + A
Sbjct: 171 VTGSAMYRVSVKLKALKKVIRDFSRDNYSDIEKRTKEAHDALLLAQSVLLASPCPSNAAI 230
Query: 186 ERNLMMKYQAALDSEENFLQQKSKVHWLQKGDGNNRFFFNYCRGRWNTNRIVGLQDP*GS 365
E K++ ++E +F Q+S+V+WL++GD N+ +F R + N I L DP G
Sbjct: 231 EAETQRKWRILAEAEASFFYQRSRVNWLREGDMNSSYFHKMASARQSLNHIHFLSDPVGD 290
Query: 366 LVTDHHGIANIAVDYYRNLLGTEK---LVEPLPDLNLPSIPDDLGR--SLIAPISSTEIL 530
+ + N V+Y+++ LG+E+ L E NL S + SL P SS +I
Sbjct: 291 RIEGQQNLENHCVEYFQSNLGSEQGLPLFEQADISNLLSYRCSPAQQVSLDTPFSSEQIK 350
Query: 531 RTLKSMKKNRSPGPDGFTPDFYIAVWDVVGSDLVNALHSFFDALDLPRQINATAISLIPK 710
S+ +N++ GPDGF+P+F+ A W ++G ++ A+H FF + L +Q NAT + LIPK
Sbjct: 351 NAFFSLPRNKASGPDGFSPEFFCACWPIIGGEVTEAIHEFFTSGKLLKQWNATNLVLIPK 410
Query: 711 VDSPVEMHQFRPISCCNVLYKCITKILANRIKPVLKNLISFNQSAFIPSRSMGDNILLSQ 890
+ + M FRPISC N +YK I+K+L +R+K L IS +QSAF+P R +N+LL+
Sbjct: 411 ITNASSMSDFRPISCLNTVYKVISKLLTDRLKDFLPAAISHSQSAFMPGRLFLENVLLAT 470
Query: 891 ALCRSYHLNKGAPRCMLKLDISKAFDSINWSFILNVLNAMHFPAKFTSWIRKCISTCMFS 1070
L Y+ AP MLK+D+ KAFDS+ W FI++ L A++ P KFT WI +C+ST FS
Sbjct: 471 ELVHGYNKKNIAPSSMLKVDLRKAFDSVRWDFIVSALRALNVPEKFTCWILECLSTASFS 530
Query: 1071 VKINGGLEGFFEGKSGVRQGDPLSPYLFVIAMEVLTCCLAS-VTSADFQFHPKCKDLKLS 1247
V +NG G F G+RQGDP+SPYLFV+AMEV + L S TS +HPK L++S
Sbjct: 531 VILNGHSAGHFWSSKGLRQGDPMSPYLFVLAMEVFSGLLQSRYTSGYIAYHPKTSQLEIS 590
Query: 1248 HLIFADDVLLFSHGDPRSVNTLLKGVDTFSRISGLHLNMQKSLIFFGNVRPADASAILQS 1427
HL+FADDV++F G S++ +++ ++ F+ SGL +N K+ ++ + +++ + + S
Sbjct: 591 HLMFADDVMIFFDGKSSSLHGIVESLEDFAGWSGLLMNTNKTQLYHAGLSQSESDS-MAS 649
Query: 1428 SNLQRGSFPFSYLGIPLVTSRINAQLCTPLIMKLCARVNSWTGRFLSFGG 1577
+ GS P YLG+PL++ ++ PLI K+ AR NSW R LSF G
Sbjct: 650 YGFKLGSLPVRYLGLPLMSRKLTIAEYAPLIEKITARFNSWVVRLLSFAG 699
>dbj|BAB09379.1| non-LTR retroelement reverse transcriptase-like protein [Arabidopsis
thaliana]
Length = 1223
Score = 371 bits (952), Expect = e-100
Identities = 201/525 (38%), Positives = 299/525 (56%), Gaps = 8/525 (1%)
Frame = +3
Query: 27 FVLTTKLKRVKQALKCL-NNSIGNVHLAVQEARNELYNLQNCIIGAPSDTQAIEERNLMM 203
F + LK +K ++ + + +GN+ EA L Q+ + PS EE
Sbjct: 285 FRFSKNLKGLKPKIRSMARDRLGNLSKKANEAYKILCAKQHVNLTNPSSMAMEEENAAYS 344
Query: 204 KYQAALDSEENFLQQKSKVHWLQKGDGNNRFFFNYCRGRWNTNRIVGLQDP*GSLVTDHH 383
++ EE +L+QKSK+HW Q GD N + F R N I + G + T
Sbjct: 345 RWDRVAILEEKYLKQKSKLHWCQVGDQNTKAFHRAAAAREAHNTIREILSNDGIVKTKGD 404
Query: 384 GIANIAVDYYRNLLGTEK------LVEPLPDLNLPSIPDDLGRSLIAPISSTEILRTLKS 545
I A ++R L + L L D +SLI P+++ EI + L
Sbjct: 405 EIKAEAERFFREFLQLIPNDFEGVTITELQQLLPVRCSDADQQSLIRPVTAEEIRKVLFR 464
Query: 546 MKKNRSPGPDGFTPDFYIAVWDVVGSDLVNALHSFFDALDLPRQINATAISLIPKVDSPV 725
M ++SPGPDG+T +F+ A W+++G + A+ SFF LP+ IN+T ++LIPK
Sbjct: 465 MPSDKSPGPDGYTSEFFKATWEIIGDEFTLAVQSFFTKGFLPKGINSTILALIPKKTEAR 524
Query: 726 EMHQFRPISCCNVLYKCITKILANRIKPVLKNLISFNQSAFIPSRSMGDNILLSQALCRS 905
EM +RPISCCNVLYK I+KI+ANR+K VL I+ NQSAF+ R + +N+LL+ L +
Sbjct: 525 EMKDYRPISCCNVLYKVISKIIANRLKLVLPKFIAGNQSAFVKDRLLIENLLLATELVKD 584
Query: 906 YHLNKGAPRCMLKLDISKAFDSINWSFILNVLNAMHFPAKFTSWIRKCISTCMFSVKING 1085
YH + + RC +K+DISKAFDS+ W F++NV + FP +F WI CI+T FSV++NG
Sbjct: 585 YHKDTISTRCAIKIDISKAFDSVQWPFLINVFTILGFPREFIHWINICITTASFSVQVNG 644
Query: 1086 GLEGFFEGKSGVRQGDPLSPYLFVIAMEVLTCCLASVTSA-DFQFHPKCKDLKLSHLIFA 1262
L G+F+ G+RQG LSPYLFVI M+VL+ L +A F +HPKCK + L+HL FA
Sbjct: 645 ELAGYFQSSRGLRQGCALSPYLFVICMDVLSKMLDKAAAARHFGYHPKCKTMGLTHLSFA 704
Query: 1263 DDVLLFSHGDPRSVNTLLKGVDTFSRISGLHLNMQKSLIFFGNVRPADASAILQSSNLQR 1442
DD+++ S G RS+ ++K D F++ SGL ++++KS ++ + + +
Sbjct: 705 DDLMVLSDGKIRSIERIIKVFDEFAKWSGLRISLEKSTVYLAGLSATARNEVADRFPFSS 764
Query: 1443 GSFPFSYLGIPLVTSRINAQLCTPLIMKLCARVNSWTGRFLSFGG 1577
G P YLG+PL+T R++ C PL+ ++ R+ SWT RFLS+ G
Sbjct: 765 GQLPVRYLGLPLITKRLSTTDCLPLLEQVRKRIGSWTSRFLSYAG 809
>gb|AAC33226.1| putative non-LTR retroelement reverse transcriptase [Arabidopsis
thaliana]
Length = 1529
Score = 369 bits (948), Expect = e-100
Identities = 204/531 (38%), Positives = 296/531 (55%), Gaps = 8/531 (1%)
Frame = +3
Query: 9 VEGDSWFVLTTKLKRVKQALKCLNNS-IGNVHLAVQEARNELYNLQNCIIGAPSDTQAIE 185
V + + + KLK +K L+ L +G++ +EA L Q + PS E
Sbjct: 576 VSTSALYRFSKKLKTLKPHLRELGKEKLGDLPKRTREAHILLCEKQATTLANPSQETIAE 635
Query: 186 ERNLMMKYQAALDSEENFLQQKSKVHWLQKGDGNNRFFFNYCRGRWNTNRIVGLQDP*GS 365
E + + EE FL+QKSK+HW+ GDGNN +F + R N I ++ P
Sbjct: 636 ELKAYTDWTHLSELEEGFLKQKSKLHWMNVGDGNNSYFHKAAQVRKMRNSIREIRGPNAE 695
Query: 366 LVTDHHGIANIAVDYYRNLLGTEK------LVEPLPDLNLPSIPDDLGRSLIAPISSTEI 527
+ I A ++ L + VE L +L L ++ EI
Sbjct: 696 TLQTSEEIKGEAERFFNEFLNRQSGDFHGISVEDLRNLMSYRCSVTDQNILTREVTGEEI 755
Query: 528 LRTLKSMKKNRSPGPDGFTPDFYIAVWDVVGSDLVNALHSFFDALDLPRQINATAISLIP 707
+ L +M N+SPGPDG+T +F+ A W + G D + A+ SFF LP+ +NAT ++LIP
Sbjct: 756 QKVLFAMPNNKSPGPDGYTSEFFKATWSLTGPDFIAAIQSFFVKGFLPKGLNATILALIP 815
Query: 708 KVDSPVEMHQFRPISCCNVLYKCITKILANRIKPVLKNLISFNQSAFIPSRSMGDNILLS 887
K D +EM +RPISCCNVLYK I+KILANR+K +L + I NQSAF+ R + +N+LL+
Sbjct: 816 KKDEAIEMKDYRPISCCNVLYKVISKILANRLKLLLPSFILQNQSAFVKERLLMENVLLA 875
Query: 888 QALCRSYHLNKGAPRCMLKLDISKAFDSINWSFILNVLNAMHFPAKFTSWIRKCISTCMF 1067
L + YH PRC +K+DISKAFDS+ W F+LN L A++FP F WI+ CIST F
Sbjct: 876 TELVKDYHKESVTPRCAMKIDISKAFDSVQWQFLLNTLEALNFPETFRHWIKLCISTATF 935
Query: 1068 SVKINGGLEGFFEGKSGVRQGDPLSPYLFVIAMEVLTCCL-ASVTSADFQFHPKCKDLKL 1244
SV++NG L GFF G+RQG LSPYLFVI M VL+ + + + +HPKC+ + L
Sbjct: 936 SVQVNGELAGFFGSSRGLRQGCALSPYLFVICMNVLSHMIDEAAVHRNIGYHPKCEKIGL 995
Query: 1245 SHLIFADDVLLFSHGDPRSVNTLLKGVDTFSRISGLHLNMQKSLIFFGNVRPADASAILQ 1424
+HL FADD+++F G S+ ++ F+ SGL ++++KS I+ V +D L
Sbjct: 996 THLCFADDLMVFVDGHQWSIEGVINVFKEFAGRSGLQISLEKSTIYLAGVSASDRVQTLS 1055
Query: 1425 SSNLQRGSFPFSYLGIPLVTSRINAQLCTPLIMKLCARVNSWTGRFLSFGG 1577
S G P YLG+PL+T ++ +PLI + +++SWT R LS+ G
Sbjct: 1056 SFPFANGQLPVRYLGLPLLTKQMTTADYSPLIEAVKTKISSWTARSLSYAG 1106
>emb|CCA65981.1| hypothetical protein [Beta vulgaris subsp. vulgaris]
Length = 1114
Score = 369 bits (946), Expect = 2e-99
Identities = 189/518 (36%), Positives = 305/518 (58%), Gaps = 6/518 (1%)
Frame = +3
Query: 42 KLKRVKQALKCLNNS-IGNVHLAVQEARNELYNLQNCIIGAPSDTQAIEERNLMMKYQAA 218
+L+ VK+ALK ++ H V+E R +L +Q + EE++L+ + +
Sbjct: 280 RLQAVKRALKSFHSKKFSKAHCQVEELRRKLAAVQALPEVSQVSELQEEEKDLIAQLRKW 339
Query: 219 LDSEENFLQQKSKVHWLQKGDGNNRFFFNYCRGRWNTNRIVGLQDP*GSLVTDHHGIANI 398
+E+ L+QKS++ WL GD N++FFF + R N+IV LQ+ G +T++ I N
Sbjct: 340 STIDESILKQKSRIQWLSLGDSNSKFFFTAIKVRKARNKIVLLQNDRGDQLTENTEIQNE 399
Query: 399 AVDYYRNLLGTEKLVEPLPDLNLPSIPDDLGRS----LIAPISSTEILRTLKSMKKNRSP 566
++YR LLGT DL++ + L + L+ PI+ EI + L + ++P
Sbjct: 400 ICNFYRRLLGTSSSQLEAIDLHVVRVGAKLSATSCAQLVQPITIQEIDQALADIDDTKAP 459
Query: 567 GPDGFTPDFYIAVWDVVGSDLVNALHSFFDALDLPRQINATAISLIPKVDSPVEMHQFRP 746
G DGF F+ W V+ ++ + FF+ + + IN TA++LIPK+D +RP
Sbjct: 460 GLDGFNSVFFKKSWLVIKQEIYEGILDFFENGFMHKPINCTAVTLIPKIDEAKHAKDYRP 519
Query: 747 ISCCNVLYKCITKILANRIKPVLKNLISFNQSAFIPSRSMGDNILLSQALCRSYHLNKGA 926
I+CC+ LYK I+KIL R++ V+ ++ Q+ FIP R +GDNILL+ L R Y+ +
Sbjct: 520 IACCSTLYKIISKILTKRLQAVITEVVDCAQTGFIPERHIGDNILLATELIRGYNRRHVS 579
Query: 927 PRCMLKLDISKAFDSINWSFILNVLNAMHFPAKFTSWIRKCISTCMFSVKINGGLEGFFE 1106
PRC++K+DI KA+DS+ W F+ ++L + FP+ F WI C+ T +S+ +NG F+
Sbjct: 580 PRCVIKVDIRKAYDSVEWVFLESMLKELGFPSMFIRWIMACVKTVSYSILLNGIPSIPFD 639
Query: 1107 GKSGVRQGDPLSPYLFVIAMEVLTCCLASV-TSADFQFHPKCKDLKLSHLIFADDVLLFS 1283
+ G+RQGDPLSP+LF ++ME L+ C+ ++ +F FHPKC+ +KL+HL+FADD+L+F+
Sbjct: 640 AQKGLRQGDPLSPFLFALSMEYLSRCMGNMCKDPEFNFHPKCERIKLTHLMFADDLLMFA 699
Query: 1284 HGDPRSVNTLLKGVDTFSRISGLHLNMQKSLIFFGNVRPADASAILQSSNLQRGSFPFSY 1463
D S++ ++ ++FS+ SGL +++KS I+FG V +A + + GS PF Y
Sbjct: 700 RADASSISKIMAAFNSFSKASGLQASIEKSCIYFGGVCHEEAEQLADRIQMPIGSLPFRY 759
Query: 1464 LGIPLVTSRINAQLCTPLIMKLCARVNSWTGRFLSFGG 1577
LG+PL + ++N C PLI K+ R W LS+ G
Sbjct: 760 LGVPLASKKLNFSQCKPLIDKITTRAQGWVAHLLSYAG 797