BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00022325
         (1719 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002523162.1| Reticuline oxidase precursor, putative [Rici...   763   0.0  
ref|XP_002523164.1| Reticuline oxidase precursor, putative [Rici...   757   0.0  
ref|XP_002317089.1| predicted protein [Populus trichocarpa] gi|2...   738   0.0  
ref|XP_003535120.1| PREDICTED: reticuline oxidase-like protein-l...   738   0.0  
ref|XP_003535119.1| PREDICTED: reticuline oxidase-like protein-l...   738   0.0  

>ref|XP_002523162.1| Reticuline oxidase precursor, putative [Ricinus communis]
            gi|223537569|gb|EEF39193.1| Reticuline oxidase precursor,
            putative [Ricinus communis]
          Length = 548

 Score =  763 bits (1971), Expect = 0.0
 Identities = 369/531 (69%), Positives = 437/531 (82%), Gaps = 1/531 (0%)
 Frame = +3

Query: 15   MASVTILPCIVILILALRAT-SNPTQDGFYQCLXXXXXXXXXXXXXXXYTPNITSFNTIL 191
            +++ +IL  + +L+L+   T S    D F QCL               YTP+ +SF+++L
Sbjct: 5    ISASSILSLLCLLLLSPSFTYSFRIFDSFIQCLKVNSEILIPFSTSL-YTPDNSSFSSVL 63

Query: 192  LSSAQNLRCVAPTMPKPELIFTPLHETHIQAAVVCAKKLGIQLRIRSGGHDYEGLSYVSE 371
             SSAQNLR ++P++PKPE IFTPLHETH+QAAV+C+K+LGI LR+RSGGHDYEGLSY SE
Sbjct: 64   QSSAQNLRYLSPSVPKPEFIFTPLHETHVQAAVICSKQLGIHLRVRSGGHDYEGLSYASE 123

Query: 372  MEPPFVVVDLSKLRSISVNIEDNSAWVQAGATIGEVYYRIAEKSKVHAFPAGLCTSLGVG 551
            +E PF+VVDLSKLR +SV+I+DNSAWVQAGAT+GE YYRI+EKS+ H FPAGLCTSLG+G
Sbjct: 124  IESPFIVVDLSKLRYVSVDIDDNSAWVQAGATVGEAYYRISEKSRTHGFPAGLCTSLGIG 183

Query: 552  GHITGGAYGPMMRKYGLGADNVLDARIIDANGKILDRKSMGEDYFWALRXXXXXSFGIIL 731
            GHITGGAYG MMRKYGLGADNV+DARIIDANGK+LDR++MGED FWA+R     SFGIIL
Sbjct: 184  GHITGGAYGSMMRKYGLGADNVIDARIIDANGKVLDRQAMGEDLFWAIRGGGGASFGIIL 243

Query: 732  SWKIKLVPIPANVTVFAVPKTLEQDGTKILYKWQQVAADKLDEDLFIRVFIQTANSSKKG 911
            +WK+KLVP+PA VTVF V KTLEQD TKILY+WQQV ADKLDEDLFIRV I TA  +K  
Sbjct: 244  AWKLKLVPVPATVTVFTVTKTLEQDATKILYRWQQV-ADKLDEDLFIRVIISTATIAKSA 302

Query: 912  ARTVQTTYQALFLGSTDRLLKVMGETFPELGLQAKHCTEMSWIESVLYIAGYPSTVPIEV 1091
            +RTV  ++Q  FLG  +RLL VM  +FPELGL  K C E SWI+SVLYIAGYPST P EV
Sbjct: 303  SRTVSNSFQGQFLGDANRLLHVMETSFPELGLTRKDCIETSWIKSVLYIAGYPSTTPPEV 362

Query: 1092 LLQGKSSFKNYFKAKSDFIKEPIPESGLEGIWTRFYEEDSPMMIWNPYGGRMGNISESEI 1271
            LLQGKS FKNYFKAKSDF+KEPIPE+GL+G+W R  +E+SP+MIWNPYGG MG ISES I
Sbjct: 363  LLQGKSLFKNYFKAKSDFVKEPIPETGLQGLWERLLQEESPLMIWNPYGGMMGKISESAI 422

Query: 1272 PFPHREGVLFKIQYLTSWYDASKDSVTKHEDWMRKLYNYMAAYASKFPRTAYVNYRDLDL 1451
            PFPHR+GVLFKIQYLTSW D  K++  KH DW+RKLYNYMA Y S FPRTAYVNYRDLDL
Sbjct: 423  PFPHRKGVLFKIQYLTSWQDGEKNA-AKHMDWIRKLYNYMAPYVSMFPRTAYVNYRDLDL 481

Query: 1452 GMNKQGNASFVEANAWGSRYYKDNFNRLVRIKTKIDPENFFRHEQSIPLLP 1604
            GMNK  + SF++A+AWGS+Y+KDNFNRLV++KTK+DP+NFFRHEQSIP LP
Sbjct: 482  GMNKNSSTSFIQASAWGSKYFKDNFNRLVQVKTKVDPDNFFRHEQSIPPLP 532


>ref|XP_002523164.1| Reticuline oxidase precursor, putative [Ricinus communis]
            gi|223537571|gb|EEF39195.1| Reticuline oxidase precursor,
            putative [Ricinus communis]
          Length = 548

 Score =  757 bits (1954), Expect = 0.0
 Identities = 368/530 (69%), Positives = 432/530 (81%), Gaps = 1/530 (0%)
 Frame = +3

Query: 18   ASVTILPCIVILILALR-ATSNPTQDGFYQCLXXXXXXXXXXXXXXXYTPNITSFNTILL 194
            +S +ILP + +L+L+   A S P  D F QCL               YT + +SF+++L 
Sbjct: 6    SSSSILPLLFLLLLSPSFAYSLPIFDSFIQCLKVNSEILIPFSTSF-YTHDNSSFSSVLQ 64

Query: 195  SSAQNLRCVAPTMPKPELIFTPLHETHIQAAVVCAKKLGIQLRIRSGGHDYEGLSYVSEM 374
            SSAQNLR + P++PKPE IFTPLHETH+QAAV+C+K+LGI LR+RSGGHDYEGLSY SE+
Sbjct: 65   SSAQNLRYLLPSVPKPEFIFTPLHETHVQAAVICSKQLGIHLRVRSGGHDYEGLSYASEI 124

Query: 375  EPPFVVVDLSKLRSISVNIEDNSAWVQAGATIGEVYYRIAEKSKVHAFPAGLCTSLGVGG 554
            E PF+VVDLSKLR +SV+I+DNSAWVQAGAT+GE YYRI+EKS+ H FPAGLCTSLG+GG
Sbjct: 125  ESPFIVVDLSKLRYVSVDIDDNSAWVQAGATVGEAYYRISEKSRTHGFPAGLCTSLGIGG 184

Query: 555  HITGGAYGPMMRKYGLGADNVLDARIIDANGKILDRKSMGEDYFWALRXXXXXSFGIILS 734
            HITGGAYG MMRKYGLGADNV+DARIIDANG++LDR++MGED FWA+R     SFGIIL+
Sbjct: 185  HITGGAYGSMMRKYGLGADNVIDARIIDANGRVLDRQAMGEDLFWAIRGGGGASFGIILA 244

Query: 735  WKIKLVPIPANVTVFAVPKTLEQDGTKILYKWQQVAADKLDEDLFIRVFIQTANSSKKGA 914
            WK+KLVP+PA VTVF V KTLEQD TKILY+WQQV ADKLDEDLFIRV I TA  +   A
Sbjct: 245  WKLKLVPVPAIVTVFTVTKTLEQDATKILYRWQQV-ADKLDEDLFIRVIISTATIANSTA 303

Query: 915  RTVQTTYQALFLGSTDRLLKVMGETFPELGLQAKHCTEMSWIESVLYIAGYPSTVPIEVL 1094
            RTV  +YQ  FLG  +RLL VM  +FPELGL  K C E SWI+SVLYIAGYPST P EVL
Sbjct: 304  RTVSNSYQGQFLGDANRLLHVMETSFPELGLTRKDCIETSWIKSVLYIAGYPSTTPPEVL 363

Query: 1095 LQGKSSFKNYFKAKSDFIKEPIPESGLEGIWTRFYEEDSPMMIWNPYGGRMGNISESEIP 1274
            LQGKS FKNYFKAKSDF+KEPIPE+ L+G+W R  +E+SP+MIWNPYGG MG ISES IP
Sbjct: 364  LQGKSLFKNYFKAKSDFVKEPIPETALQGLWKRLLQEESPLMIWNPYGGMMGKISESAIP 423

Query: 1275 FPHREGVLFKIQYLTSWYDASKDSVTKHEDWMRKLYNYMAAYASKFPRTAYVNYRDLDLG 1454
            FPHR+GVL KIQYLT W D  K++  KH DW+RKLYNYMA Y S FPRTAYVNYRDLDLG
Sbjct: 424  FPHRKGVLCKIQYLTGWQDGEKNA-AKHMDWIRKLYNYMAPYVSMFPRTAYVNYRDLDLG 482

Query: 1455 MNKQGNASFVEANAWGSRYYKDNFNRLVRIKTKIDPENFFRHEQSIPLLP 1604
            MNK  + SF++A+AWGS+Y+KDNFNRLV++KTK+DP+NFFRHEQSIP LP
Sbjct: 483  MNKNSSTSFIQASAWGSKYFKDNFNRLVQVKTKVDPDNFFRHEQSIPPLP 532


>ref|XP_002317089.1| predicted protein [Populus trichocarpa] gi|222860154|gb|EEE97701.1|
            predicted protein [Populus trichocarpa]
          Length = 545

 Score =  738 bits (1905), Expect = 0.0
 Identities = 355/531 (66%), Positives = 429/531 (80%), Gaps = 2/531 (0%)
 Frame = +3

Query: 18   ASVTILPCIVILILALRAT-SNPTQDGFYQCLXXXXXXXXXXXXXXXYTP-NITSFNTIL 191
            +S  I   +V+L+L+   T S P +D F QC                YTP N +SF+ +L
Sbjct: 4    SSFCIFSTLVVLLLSSSVTVSLPIKDDFVQCFLQNSEISVPTSSF--YTPDNSSSFSAVL 61

Query: 192  LSSAQNLRCVAPTMPKPELIFTPLHETHIQAAVVCAKKLGIQLRIRSGGHDYEGLSYVSE 371
             S+AQNLR + P++PKP  IFTPL+E H+QA+V+C ++LG+ LR+RSGGHDYEGLSY SE
Sbjct: 62   QSTAQNLRYLLPSVPKPVFIFTPLYEAHVQASVICCRQLGLHLRVRSGGHDYEGLSYASE 121

Query: 372  MEPPFVVVDLSKLRSISVNIEDNSAWVQAGATIGEVYYRIAEKSKVHAFPAGLCTSLGVG 551
            +E PF+VVDL+KLR+I VNIEDNSAWVQAGATIGE+YYRIAEKSK H FPAGLC+SLG+G
Sbjct: 122  IESPFIVVDLAKLRAIQVNIEDNSAWVQAGATIGELYYRIAEKSKTHGFPAGLCSSLGIG 181

Query: 552  GHITGGAYGPMMRKYGLGADNVLDARIIDANGKILDRKSMGEDYFWALRXXXXXSFGIIL 731
            GHITGGAYG MMRKYGLGADNV+DAR+IDA+G++L+R+SMGED+FWA+R     SFGII 
Sbjct: 182  GHITGGAYGSMMRKYGLGADNVIDARVIDASGRVLERQSMGEDFFWAIRGGGGASFGIIT 241

Query: 732  SWKIKLVPIPANVTVFAVPKTLEQDGTKILYKWQQVAADKLDEDLFIRVFIQTANSSKKG 911
            +WK+KLVP+P+ VTVF V KTLEQ GTK+LY+WQQV ADKLDEDLFIRV IQTAN +KKG
Sbjct: 242  AWKVKLVPVPSTVTVFTVTKTLEQGGTKLLYRWQQV-ADKLDEDLFIRVIIQTANGAKKG 300

Query: 912  ARTVQTTYQALFLGSTDRLLKVMGETFPELGLQAKHCTEMSWIESVLYIAGYPSTVPIEV 1091
             RTV T+Y ALFLG   RLL VM   FPELGL  K C E +W++SVLYIAGYP+  P EV
Sbjct: 301  QRTVTTSYNALFLGDASRLLNVMKTGFPELGLTRKDCVETTWLKSVLYIAGYPANTPPEV 360

Query: 1092 LLQGKSSFKNYFKAKSDFIKEPIPESGLEGIWTRFYEEDSPMMIWNPYGGRMGNISESEI 1271
            LLQGKS+FKNYFKAKSDF++ PIPE+ LEGIW R+++E +P MIWNPYGG M  ISES I
Sbjct: 361  LLQGKSTFKNYFKAKSDFVQHPIPEAALEGIWKRYFQEVTPFMIWNPYGGMMSKISESSI 420

Query: 1272 PFPHREGVLFKIQYLTSWYDASKDSVTKHEDWMRKLYNYMAAYASKFPRTAYVNYRDLDL 1451
            PFPHR+GVLFKIQYLTSW   ++D+ +KH +W+RKLY YMA Y S FPR AYVNYRDLDL
Sbjct: 421  PFPHRKGVLFKIQYLTSWQTPAEDA-SKHINWIRKLYKYMAPYVSMFPRQAYVNYRDLDL 479

Query: 1452 GMNKQGNASFVEANAWGSRYYKDNFNRLVRIKTKIDPENFFRHEQSIPLLP 1604
            GMNK+ N SF EA+ WG++Y+K+NFNRLV++KT +DP+NFFRHEQSIP LP
Sbjct: 480  GMNKKRNTSFKEASVWGTKYFKNNFNRLVQVKTTVDPDNFFRHEQSIPPLP 530


>ref|XP_003535120.1| PREDICTED: reticuline oxidase-like protein-like isoform 2 [Glycine
            max]
          Length = 540

 Score =  738 bits (1904), Expect = 0.0
 Identities = 356/525 (67%), Positives = 419/525 (79%)
 Frame = +3

Query: 30   ILPCIVILILALRATSNPTQDGFYQCLXXXXXXXXXXXXXXXYTPNITSFNTILLSSAQN 209
            +L  +++L+    A S   ++ F QCL               YTP   SFN IL SSAQN
Sbjct: 4    LLVTLILLLSVSLAASASLEENFVQCLSFYSDKAAPFYASI-YTPQNASFNKILESSAQN 62

Query: 210  LRCVAPTMPKPELIFTPLHETHIQAAVVCAKKLGIQLRIRSGGHDYEGLSYVSEMEPPFV 389
            LR + P+ PKPELIFTPL ++H+Q AV C+KKLGI LRIRSGGHDYEGLSYVSE+E PF+
Sbjct: 63   LRYLVPSAPKPELIFTPLTDSHVQVAVTCSKKLGIHLRIRSGGHDYEGLSYVSEVESPFI 122

Query: 390  VVDLSKLRSISVNIEDNSAWVQAGATIGEVYYRIAEKSKVHAFPAGLCTSLGVGGHITGG 569
            +VDLSKLR+I V+IEDN+AW+QAGATIGEVYYRI EKS VH FPAGLCTSLGVGGHITGG
Sbjct: 123  IVDLSKLRAIDVDIEDNTAWIQAGATIGEVYYRIYEKSSVHGFPAGLCTSLGVGGHITGG 182

Query: 570  AYGPMMRKYGLGADNVLDARIIDANGKILDRKSMGEDYFWALRXXXXXSFGIILSWKIKL 749
            AYG MMRKYGLGADNV+DARI+DANG+ILDR++MGED FWA+R     SFGI+L WKIKL
Sbjct: 183  AYGSMMRKYGLGADNVVDARIVDANGQILDREAMGEDLFWAIRGGGGASFGILLWWKIKL 242

Query: 750  VPIPANVTVFAVPKTLEQDGTKILYKWQQVAADKLDEDLFIRVFIQTANSSKKGARTVQT 929
            VP+P  VTVF V ++LEQD TKIL++WQ+V A  +DEDLFIRV IQ A    K  RT+ T
Sbjct: 243  VPVPETVTVFTVTRSLEQDATKILHRWQEV-APYIDEDLFIRVIIQPATVGNKTERTITT 301

Query: 930  TYQALFLGSTDRLLKVMGETFPELGLQAKHCTEMSWIESVLYIAGYPSTVPIEVLLQGKS 1109
            +Y A FLG  DRLL+VM E+FPELGL  K C E SWI+SVLYIAGYP+  P EVLLQGKS
Sbjct: 302  SYNAQFLGGADRLLQVMKESFPELGLTKKDCLETSWIKSVLYIAGYPNDTPPEVLLQGKS 361

Query: 1110 SFKNYFKAKSDFIKEPIPESGLEGIWTRFYEEDSPMMIWNPYGGRMGNISESEIPFPHRE 1289
            +FKNYFKAKSDF+++PIPE+GLEG+W R  EEDSP+MIWNPYGG M   SES+IPFPHR 
Sbjct: 362  TFKNYFKAKSDFVRDPIPETGLEGLWQRLLEEDSPLMIWNPYGGMMSKFSESDIPFPHRN 421

Query: 1290 GVLFKIQYLTSWYDASKDSVTKHEDWMRKLYNYMAAYASKFPRTAYVNYRDLDLGMNKQG 1469
            G L+KIQYLT W D  K++ +KHEDW+RKLYNYM  Y SKFPR AYVNYRDLDLGMNK+ 
Sbjct: 422  GTLYKIQYLTLWQDGDKNA-SKHEDWIRKLYNYMTPYVSKFPREAYVNYRDLDLGMNKKN 480

Query: 1470 NASFVEANAWGSRYYKDNFNRLVRIKTKIDPENFFRHEQSIPLLP 1604
            + S+++A AWG+ Y+KDNFNRLV+IKTK+DP+N FRHEQSIP LP
Sbjct: 481  STSYIQATAWGNMYFKDNFNRLVKIKTKVDPDNVFRHEQSIPPLP 525


>ref|XP_003535119.1| PREDICTED: reticuline oxidase-like protein-like isoform 1 [Glycine
            max]
          Length = 543

 Score =  738 bits (1904), Expect = 0.0
 Identities = 356/530 (67%), Positives = 422/530 (79%)
 Frame = +3

Query: 15   MASVTILPCIVILILALRATSNPTQDGFYQCLXXXXXXXXXXXXXXXYTPNITSFNTILL 194
            ++ ++ L  +++L+    A S   ++ F QCL               YTP   SFN IL 
Sbjct: 2    VSPISHLVTLILLLSVSLAASASLEENFVQCLSFYSDKAAPFYASI-YTPQNASFNKILE 60

Query: 195  SSAQNLRCVAPTMPKPELIFTPLHETHIQAAVVCAKKLGIQLRIRSGGHDYEGLSYVSEM 374
            SSAQNLR + P+ PKPELIFTPL ++H+Q AV C+KKLGI LRIRSGGHDYEGLSYVSE+
Sbjct: 61   SSAQNLRYLVPSAPKPELIFTPLTDSHVQVAVTCSKKLGIHLRIRSGGHDYEGLSYVSEV 120

Query: 375  EPPFVVVDLSKLRSISVNIEDNSAWVQAGATIGEVYYRIAEKSKVHAFPAGLCTSLGVGG 554
            E PF++VDLSKLR+I V+IEDN+AW+QAGATIGEVYYRI EKS VH FPAGLCTSLGVGG
Sbjct: 121  ESPFIIVDLSKLRAIDVDIEDNTAWIQAGATIGEVYYRIYEKSSVHGFPAGLCTSLGVGG 180

Query: 555  HITGGAYGPMMRKYGLGADNVLDARIIDANGKILDRKSMGEDYFWALRXXXXXSFGIILS 734
            HITGGAYG MMRKYGLGADNV+DARI+DANG+ILDR++MGED FWA+R     SFGI+L 
Sbjct: 181  HITGGAYGSMMRKYGLGADNVVDARIVDANGQILDREAMGEDLFWAIRGGGGASFGILLW 240

Query: 735  WKIKLVPIPANVTVFAVPKTLEQDGTKILYKWQQVAADKLDEDLFIRVFIQTANSSKKGA 914
            WKIKLVP+P  VTVF V ++LEQD TKIL++WQ+V A  +DEDLFIRV IQ A    K  
Sbjct: 241  WKIKLVPVPETVTVFTVTRSLEQDATKILHRWQEV-APYIDEDLFIRVIIQPATVGNKTE 299

Query: 915  RTVQTTYQALFLGSTDRLLKVMGETFPELGLQAKHCTEMSWIESVLYIAGYPSTVPIEVL 1094
            RT+ T+Y A FLG  DRLL+VM E+FPELGL  K C E SWI+SVLYIAGYP+  P EVL
Sbjct: 300  RTITTSYNAQFLGGADRLLQVMKESFPELGLTKKDCLETSWIKSVLYIAGYPNDTPPEVL 359

Query: 1095 LQGKSSFKNYFKAKSDFIKEPIPESGLEGIWTRFYEEDSPMMIWNPYGGRMGNISESEIP 1274
            LQGKS+FKNYFKAKSDF+++PIPE+GLEG+W R  EEDSP+MIWNPYGG M   SES+IP
Sbjct: 360  LQGKSTFKNYFKAKSDFVRDPIPETGLEGLWQRLLEEDSPLMIWNPYGGMMSKFSESDIP 419

Query: 1275 FPHREGVLFKIQYLTSWYDASKDSVTKHEDWMRKLYNYMAAYASKFPRTAYVNYRDLDLG 1454
            FPHR G L+KIQYLT W D  K++ +KHEDW+RKLYNYM  Y SKFPR AYVNYRDLDLG
Sbjct: 420  FPHRNGTLYKIQYLTLWQDGDKNA-SKHEDWIRKLYNYMTPYVSKFPREAYVNYRDLDLG 478

Query: 1455 MNKQGNASFVEANAWGSRYYKDNFNRLVRIKTKIDPENFFRHEQSIPLLP 1604
            MNK+ + S+++A AWG+ Y+KDNFNRLV+IKTK+DP+N FRHEQSIP LP
Sbjct: 479  MNKKNSTSYIQATAWGNMYFKDNFNRLVKIKTKVDPDNVFRHEQSIPPLP 528