BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00021905
         (2395 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002269354.1| PREDICTED: K(+) efflux antiporter 5 [Vitis v...   756   0.0  
ref|XP_004148124.1| PREDICTED: K(+) efflux antiporter 5-like [Cu...   733   0.0  
ref|XP_002510895.1| Glutathione-regulated potassium-efflux syste...   728   0.0  
ref|NP_001119415.1| K(+) efflux antiporter 5 [Arabidopsis thalia...   722   0.0  
ref|XP_003549141.1| PREDICTED: K(+) efflux antiporter 5-like [Gl...   721   0.0  

>ref|XP_002269354.1| PREDICTED: K(+) efflux antiporter 5 [Vitis vinifera]
            gi|296084106|emb|CBI24494.3| unnamed protein product
            [Vitis vinifera]
          Length = 577

 Score =  756 bits (1952), Expect = 0.0
 Identities = 401/569 (70%), Positives = 453/569 (79%), Gaps = 6/569 (1%)
 Frame = -2

Query: 2208 LWICVVLIIVFCAPTSLSARSEQEIRERFYGNLVNSTAPDSGDGSIAKMFDRVLEKEFSE 2029
            LW+C+V+ ++ C    +S RS+QEIR+RFYGNL+N TAPDSGDGSIAKMFDRVLEKEFSE
Sbjct: 10   LWLCLVVTVI-CPRHCVSVRSDQEIRDRFYGNLLNGTAPDSGDGSIAKMFDRVLEKEFSE 68

Query: 2028 -----NDQPEGKDKSSFNSSVADKEAVLETVAKISHEKMKKNESQETNGTRAF-FQDVFS 1867
                 NDQPEG + SSFNSSVAD++AVLETVAKI+HEK K+N++QE NGTR+F FQDVFS
Sbjct: 69   KEFSENDQPEGSNGSSFNSSVADQQAVLETVAKITHEKAKRNDTQEANGTRSFQFQDVFS 128

Query: 1866 VEKEGSEDMDTIIDSKDNTFVMSNKKSKYPVLQVDLRLISDLXXXXXXXXXXXXIFSCLG 1687
            ++ EG++D+ T+ID KDN FVMSNKKSKYPVLQVDLRLISDL            IFSCLG
Sbjct: 129  LDNEGTDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCLG 188

Query: 1686 QPVIVGYLLAGSLIGPGGLKFISEMVQVETVAQXXXXXXXXXXXXXFSLTKLKXXXXXXX 1507
            QPVIVGYLLAGS+IGPGGLKFISEMVQVETVAQ             FSL KLK       
Sbjct: 189  QPVIVGYLLAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLPKLKAVGPVAV 248

Query: 1506 XXXXLQILIFMFLCGITALLCGAKLSEGLFVGCFLSMSSTAVVLKFLVEKNSNNAVHGQV 1327
                LQI IFMFLCG+TA+LCGAKLSEG+FVG FLSMSSTAVV+KFLVE+N+NNA+HGQV
Sbjct: 249  LGGLLQIFIFMFLCGVTAMLCGAKLSEGVFVGSFLSMSSTAVVVKFLVERNTNNALHGQV 308

Query: 1326 TIGTLIFQDCTVGLLFALLPVLGGNSGILQGMASMGKVVLTLSAYIFAASVLTWSFVPRF 1147
            TIGTLI QDC VGLLFALLPVLGGNSG+LQGM SMGK++L LS ++ AASVL+WSFVPRF
Sbjct: 309  TIGTLILQDCAVGLLFALLPVLGGNSGLLQGMISMGKLLLVLSMFLTAASVLSWSFVPRF 368

Query: 1146 LKLMIQLSSQTNELYQLAMVAFCLLSATCSDKLGLSLELGSFVAGVMISTTEFAQHTLDQ 967
            LKLMIQLSSQTNELYQLA VAFCLLSA CSDKLGLSLELGSFVAGVMISTT+FAQHTLDQ
Sbjct: 369  LKLMIQLSSQTNELYQLASVAFCLLSAWCSDKLGLSLELGSFVAGVMISTTDFAQHTLDQ 428

Query: 966  VEPVRNLFAALFLSSIGMLIHVHFLWTHXXXXXXXXXXXXXXXXXXXXXXXKGFRYGTRT 787
            VEP+RNLFAALFLSSIGMLIHV FLWTH                       K F Y  RT
Sbjct: 429  VEPIRNLFAALFLSSIGMLIHVQFLWTHVDILLASVILVVVVKTAVVAVVTKAFGYSVRT 488

Query: 786  SFLVGVMLAQIGEFAFVLLSRASNLQIVEGKMYXXXXXXXXXXXXXTPVVFKLIPAMVHL 607
            SFLVGV+LAQIGEFAFVLLSRASNL+++EGKMY             TPV+FKLIPA++HL
Sbjct: 489  SFLVGVLLAQIGEFAFVLLSRASNLKLIEGKMYLLLLGTTALSLVTTPVLFKLIPAVMHL 548

Query: 606  GVLMHWFPPESSTETEEKVSMIEVHNRLL 520
            GVLMHWFP E+S + EE+ S++E  +RLL
Sbjct: 549  GVLMHWFPSENSNQNEERASVLESRDRLL 577


>ref|XP_004148124.1| PREDICTED: K(+) efflux antiporter 5-like [Cucumis sativus]
            gi|449499664|ref|XP_004160879.1| PREDICTED: K(+) efflux
            antiporter 5-like [Cucumis sativus]
          Length = 578

 Score =  733 bits (1891), Expect = 0.0
 Identities = 387/559 (69%), Positives = 442/559 (79%), Gaps = 1/559 (0%)
 Frame = -2

Query: 2193 VLIIVFCAPTSLSARSEQEIRERFYGNLVNSTAPDSGDGSIAKMFDRVLEKEFSENDQPE 2014
            +L+++  +   ++ARS++EIRERFYGNL+NSTAP SGDGS A+MFD+VLEKEFS+ND PE
Sbjct: 20   ILVVLISSHIHVTARSDKEIRERFYGNLINSTAPTSGDGSFAQMFDKVLEKEFSDNDLPE 79

Query: 2013 GKDKSSFNSSVADKEAVLETVAKISHEKMKKNESQETNGTRAF-FQDVFSVEKEGSEDMD 1837
            G   SSFNSSVAD+EA LETVAKI+HEK KKN+SQ+ NGTRAF  QDVFS+E E S+D+ 
Sbjct: 80   GSGGSSFNSSVADQEAELETVAKITHEKGKKNDSQKANGTRAFQLQDVFSLENEESDDVT 139

Query: 1836 TIIDSKDNTFVMSNKKSKYPVLQVDLRLISDLXXXXXXXXXXXXIFSCLGQPVIVGYLLA 1657
            T+ID KDN FVMSNKKSKYPVLQVDLRLISDL            IFSCLGQPVIVGYLLA
Sbjct: 140  TLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIIFSCLGQPVIVGYLLA 199

Query: 1656 GSLIGPGGLKFISEMVQVETVAQXXXXXXXXXXXXXFSLTKLKXXXXXXXXXXXLQILIF 1477
            GS+IGPGGLKFISEMVQVETVAQ             FSLTKLK           LQI+IF
Sbjct: 200  GSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKVVGAVAIFGGFLQIIIF 259

Query: 1476 MFLCGITALLCGAKLSEGLFVGCFLSMSSTAVVLKFLVEKNSNNAVHGQVTIGTLIFQDC 1297
            MFLCGI A+L GAKLSEG+FVG FLSMSSTAVV+KFLVE+NS+N +HGQVTIGTLI QDC
Sbjct: 260  MFLCGIVAMLSGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSSNTLHGQVTIGTLILQDC 319

Query: 1296 TVGLLFALLPVLGGNSGILQGMASMGKVVLTLSAYIFAASVLTWSFVPRFLKLMIQLSSQ 1117
             VGLLFALLPVLGG++G++ GM SMGK++L LS Y+ AAS+L+WSFVPRFLKLM+QLSSQ
Sbjct: 320  AVGLLFALLPVLGGHNGLILGMISMGKLLLVLSVYLTAASILSWSFVPRFLKLMMQLSSQ 379

Query: 1116 TNELYQLAMVAFCLLSATCSDKLGLSLELGSFVAGVMISTTEFAQHTLDQVEPVRNLFAA 937
            TNELYQLA VAFCLLSA CSDKLGLSLELGSFVAGVM+STT+F QHTLDQVEP+RNLFAA
Sbjct: 380  TNELYQLAAVAFCLLSAWCSDKLGLSLELGSFVAGVMVSTTDFGQHTLDQVEPIRNLFAA 439

Query: 936  LFLSSIGMLIHVHFLWTHXXXXXXXXXXXXXXXXXXXXXXXKGFRYGTRTSFLVGVMLAQ 757
            LFLSSIGMLIHVHFLW+H                       K F YG RTSF VGVMLAQ
Sbjct: 440  LFLSSIGMLIHVHFLWSHLDILLASVMLVVFVKTAVATIVAKAFGYGIRTSFQVGVMLAQ 499

Query: 756  IGEFAFVLLSRASNLQIVEGKMYXXXXXXXXXXXXXTPVVFKLIPAMVHLGVLMHWFPPE 577
            IGEFAFVLLSRASNL ++ GK+Y             TP++FKLIPA+++LGVLMHWFP E
Sbjct: 500  IGEFAFVLLSRASNLHLIGGKVYLLLLGTTALSLVTTPLLFKLIPAVLNLGVLMHWFPSE 559

Query: 576  SSTETEEKVSMIEVHNRLL 520
            ++ + EEKVSMIE HNR+L
Sbjct: 560  NNIQNEEKVSMIEAHNRML 578


>ref|XP_002510895.1| Glutathione-regulated potassium-efflux system protein kefB, putative
            [Ricinus communis] gi|223550010|gb|EEF51497.1|
            Glutathione-regulated potassium-efflux system protein
            kefB, putative [Ricinus communis]
          Length = 565

 Score =  728 bits (1880), Expect = 0.0
 Identities = 385/559 (68%), Positives = 442/559 (79%), Gaps = 1/559 (0%)
 Frame = -2

Query: 2211 RLWICVVLIIVFCAPTSLSARSEQEIRERFYGNLVNSTAPDSGDGSIAKMFDRVLEKEFS 2032
            R W+  +L+++     S+SARS++E+RERFYGNL+NS+APD+GDGSIA+MFDRVL+KEFS
Sbjct: 8    RYWLLSLLVLLISTAISVSARSDKEMRERFYGNLLNSSAPDNGDGSIAQMFDRVLQKEFS 67

Query: 2031 ENDQPEGKDKSSFNSSVADKEAVLETVAKISHEKMKKNESQETNGTRAF-FQDVFSVEKE 1855
            ENDQPEG D SSFNSSVAD++AVLETV KI+  K KKN++Q  NGT++F FQDVF +E E
Sbjct: 68   ENDQPEGSDGSSFNSSVADEQAVLETVVKIN--KAKKNDTQVANGTKSFGFQDVFKLENE 125

Query: 1854 GSEDMDTIIDSKDNTFVMSNKKSKYPVLQVDLRLISDLXXXXXXXXXXXXIFSCLGQPVI 1675
             SEDM T+ID KDN FVMSNKKSKYPVLQVDLRLISDL            IFSCLGQP+I
Sbjct: 126  DSEDMTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVAIVSAAIGGIIFSCLGQPII 185

Query: 1674 VGYLLAGSLIGPGGLKFISEMVQVETVAQXXXXXXXXXXXXXFSLTKLKXXXXXXXXXXX 1495
            VGY+LAGS+IGPGGLKFISEMVQVETVAQ             FSLTKLK           
Sbjct: 186  VGYILAGSIIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLTKLKAVGPVAVVGGL 245

Query: 1494 LQILIFMFLCGITALLCGAKLSEGLFVGCFLSMSSTAVVLKFLVEKNSNNAVHGQVTIGT 1315
            LQI+IFMFLCG+TA+LCGAKLSEG+FVG FLSMSSTAVV+KFLVE+NS+N++HGQVTIGT
Sbjct: 246  LQIVIFMFLCGVTAVLCGAKLSEGVFVGFFLSMSSTAVVVKFLVERNSSNSLHGQVTIGT 305

Query: 1314 LIFQDCTVGLLFALLPVLGGNSGILQGMASMGKVVLTLSAYIFAASVLTWSFVPRFLKLM 1135
            LIFQDC VGLLFALLPVLGG+SG+LQGM SMGK++L LS ++  AS+L+WSFVPRFLKLM
Sbjct: 306  LIFQDCAVGLLFALLPVLGGSSGMLQGMVSMGKLLLVLSIFLIVASILSWSFVPRFLKLM 365

Query: 1134 IQLSSQTNELYQLAMVAFCLLSATCSDKLGLSLELGSFVAGVMISTTEFAQHTLDQVEPV 955
            IQLSSQTNELYQLA+VAFCLLSA CSDKLGLSLELGSFVAGVMISTT+FAQHTLDQVEP+
Sbjct: 366  IQLSSQTNELYQLAVVAFCLLSAWCSDKLGLSLELGSFVAGVMISTTDFAQHTLDQVEPI 425

Query: 954  RNLFAALFLSSIGMLIHVHFLWTHXXXXXXXXXXXXXXXXXXXXXXXKGFRYGTRTSFLV 775
            RNLFAALFLS IGMLIHVHFLW H                       K F Y  RTSF V
Sbjct: 426  RNLFAALFLSGIGMLIHVHFLWNHVDILLASVILVIVVKTAVAAVVTKAFGYSIRTSFHV 485

Query: 774  GVMLAQIGEFAFVLLSRASNLQIVEGKMYXXXXXXXXXXXXXTPVVFKLIPAMVHLGVLM 595
            GV+LAQIGEFAFVLLSRASNL +VEGKMY             TP++FKLIP +++LGVLM
Sbjct: 486  GVLLAQIGEFAFVLLSRASNLHLVEGKMYLLLLGTTALSLVTTPLLFKLIPNVMNLGVLM 545

Query: 594  HWFPPESSTETEEKVSMIE 538
             WFPPESST+ E +  ++E
Sbjct: 546  QWFPPESSTQNEHEWLLLE 564


>ref|NP_001119415.1| K(+) efflux antiporter 5 [Arabidopsis thaliana]
            gi|332008735|gb|AED96118.1| K(+) efflux antiporter 5
            [Arabidopsis thaliana]
          Length = 565

 Score =  722 bits (1863), Expect = 0.0
 Identities = 381/548 (69%), Positives = 435/548 (79%), Gaps = 1/548 (0%)
 Frame = -2

Query: 2166 TSLSARSEQEIRERFYGNLVNSTAPDSGDGSIAKMFDRVLEKEFSENDQPEGKDKSSFNS 1987
            TSLSARS++E RERFYGN+VNSTAP +G+GSIAKMFDRVLEKEFSEND PEG D +SFNS
Sbjct: 17   TSLSARSDEETRERFYGNVVNSTAPGNGEGSIAKMFDRVLEKEFSENDSPEGSDGASFNS 76

Query: 1986 SVADKEAVLETVAKISHEKMKKNESQETNGTRAF-FQDVFSVEKEGSEDMDTIIDSKDNT 1810
            SVAD++A +ETVAK++HEK K+N++QE NGTR F  QDVFS+E E S+DM T+ID K+N 
Sbjct: 77   SVADQQAEIETVAKVTHEKGKRNDTQENNGTRPFQLQDVFSLENEDSDDM-TLIDKKNNV 135

Query: 1809 FVMSNKKSKYPVLQVDLRLISDLXXXXXXXXXXXXIFSCLGQPVIVGYLLAGSLIGPGGL 1630
            FVMSNKKSKYP+LQVDLRLISDL            +FSCLGQPVIVGYLLAGS+IGPGGL
Sbjct: 136  FVMSNKKSKYPILQVDLRLISDLVVIIVFAAIGGIVFSCLGQPVIVGYLLAGSIIGPGGL 195

Query: 1629 KFISEMVQVETVAQXXXXXXXXXXXXXFSLTKLKXXXXXXXXXXXLQILIFMFLCGITAL 1450
            KFISEMVQVETVAQ             FS+TKLK           LQI++ MFLCG+TAL
Sbjct: 196  KFISEMVQVETVAQFGVVFLLFALGLEFSMTKLKVVGPVAVLGGLLQIVLLMFLCGVTAL 255

Query: 1449 LCGAKLSEGLFVGCFLSMSSTAVVLKFLVEKNSNNAVHGQVTIGTLIFQDCTVGLLFALL 1270
            LCGA+LSEG+FVG FLSMSSTAVV+KFLVE+NS +++HGQVTIG LIFQDC VGLLFALL
Sbjct: 256  LCGARLSEGIFVGAFLSMSSTAVVVKFLVERNSTSSLHGQVTIGILIFQDCVVGLLFALL 315

Query: 1269 PVLGGNSGILQGMASMGKVVLTLSAYIFAASVLTWSFVPRFLKLMIQLSSQTNELYQLAM 1090
            PVLGGNSG+LQG+ SMGK++L LS Y+  AS+LTWSFVPRFLKLMIQLSSQTNELYQLA 
Sbjct: 316  PVLGGNSGLLQGIISMGKLLLILSIYLTVASLLTWSFVPRFLKLMIQLSSQTNELYQLAA 375

Query: 1089 VAFCLLSATCSDKLGLSLELGSFVAGVMISTTEFAQHTLDQVEPVRNLFAALFLSSIGML 910
            VAFCLLSA CSDKLGLSLELGSFVAGVM+STTEFAQHTL+QVEP+RNLFAALFLSSIGML
Sbjct: 376  VAFCLLSAWCSDKLGLSLELGSFVAGVMLSTTEFAQHTLEQVEPIRNLFAALFLSSIGML 435

Query: 909  IHVHFLWTHXXXXXXXXXXXXXXXXXXXXXXXKGFRYGTRTSFLVGVMLAQIGEFAFVLL 730
            I+VHFLW H                       K FRY  R SF VGV+LAQIGEFAFVLL
Sbjct: 436  INVHFLWNHVDILLASVILVIVIKTAIAAVVVKAFRYNMRISFHVGVLLAQIGEFAFVLL 495

Query: 729  SRASNLQIVEGKMYXXXXXXXXXXXXXTPVVFKLIPAMVHLGVLMHWFPPESSTETEEKV 550
            SRASNL ++EGKMY             TP++FKLIP+ ++LGVL+ WFP E+S+  EEK 
Sbjct: 496  SRASNLHVIEGKMYLLLLGTTALSLVTTPLLFKLIPSAMNLGVLLRWFPSENSSPNEEKA 555

Query: 549  SMIEVHNR 526
            S+IEVHNR
Sbjct: 556  SLIEVHNR 563


>ref|XP_003549141.1| PREDICTED: K(+) efflux antiporter 5-like [Glycine max]
          Length = 576

 Score =  721 bits (1862), Expect = 0.0
 Identities = 390/573 (68%), Positives = 444/573 (77%), Gaps = 1/573 (0%)
 Frame = -2

Query: 2235 ACAKRESCRLWICVVLIIVFCAPTSLSARSEQEIRERFYGNLVNSTAPDSGDGSIAKMFD 2056
            A  KR +  LW CV L +  CA    +ARS+QE RERFYGN+ N ++P+S + ++AKMFD
Sbjct: 7    ATKKRRAFGLWCCV-LCLAICARVCGAARSDQETRERFYGNMPNGSSPESNN-TLAKMFD 64

Query: 2055 RVLEKEFSENDQPEGKDKSSFNSSVADKEAVLETVAKISHEKMKKNESQETNGTRAF-FQ 1879
            RVLEKEFSENDQPE  DK+SFNSSVAD++AVLETVAKI+H+K K+NE+ E N TRAF FQ
Sbjct: 65   RVLEKEFSENDQPEEPDKNSFNSSVADQQAVLETVAKITHDKAKRNETHEGNATRAFQFQ 124

Query: 1878 DVFSVEKEGSEDMDTIIDSKDNTFVMSNKKSKYPVLQVDLRLISDLXXXXXXXXXXXXIF 1699
            DVFS+E E S+D+ T+ID KDN FVMSNKKSKYPVLQVDLRLISDL            +F
Sbjct: 125  DVFSLENEDSDDVTTLIDKKDNVFVMSNKKSKYPVLQVDLRLISDLVVVIVSAAIGGIVF 184

Query: 1698 SCLGQPVIVGYLLAGSLIGPGGLKFISEMVQVETVAQXXXXXXXXXXXXXFSLTKLKXXX 1519
            SCLGQPVIVGYLLAGSLIGPGGLKFISEMVQVETVAQ             FSL KLK   
Sbjct: 185  SCLGQPVIVGYLLAGSLIGPGGLKFISEMVQVETVAQFGVVFLLFALGLEFSLAKLKAVG 244

Query: 1518 XXXXXXXXLQILIFMFLCGITALLCGAKLSEGLFVGCFLSMSSTAVVLKFLVEKNSNNAV 1339
                    LQI+IFMF+CGI ++L GAKLSEG+FVG FLSMSSTAVV+KFLVE+NSNNA+
Sbjct: 245  PVAVLGGLLQIIIFMFMCGILSMLFGAKLSEGVFVGSFLSMSSTAVVVKFLVERNSNNAL 304

Query: 1338 HGQVTIGTLIFQDCTVGLLFALLPVLGGNSGILQGMASMGKVVLTLSAYIFAASVLTWSF 1159
            H QVTIGTLIFQDC VGLLFALLPVLGGNSG+LQG+ SMGK++L LS YI A SVL+W+F
Sbjct: 305  HVQVTIGTLIFQDCAVGLLFALLPVLGGNSGLLQGLMSMGKLLLVLSLYITATSVLSWTF 364

Query: 1158 VPRFLKLMIQLSSQTNELYQLAMVAFCLLSATCSDKLGLSLELGSFVAGVMISTTEFAQH 979
            VPRFLKLM++LSSQTNELYQLA VAFCLLSA CSDKLGLSLELGSF+AGVMISTT+FAQH
Sbjct: 365  VPRFLKLMMRLSSQTNELYQLAAVAFCLLSAWCSDKLGLSLELGSFMAGVMISTTDFAQH 424

Query: 978  TLDQVEPVRNLFAALFLSSIGMLIHVHFLWTHXXXXXXXXXXXXXXXXXXXXXXXKGFRY 799
            TLDQVEP+RNLFAALFLSSIGMLIHVHFLW H                       K F Y
Sbjct: 425  TLDQVEPIRNLFAALFLSSIGMLIHVHFLWNHVDILLASVILVVVVKTAVAVIVTKAFGY 484

Query: 798  GTRTSFLVGVMLAQIGEFAFVLLSRASNLQIVEGKMYXXXXXXXXXXXXXTPVVFKLIPA 619
              +TSF+VG+ LAQIGEFAFVLLSRASNL +VEGKMY             TP++FKLIPA
Sbjct: 485  SLKTSFIVGISLAQIGEFAFVLLSRASNLHLVEGKMYLLLLGTTALSLVTTPLLFKLIPA 544

Query: 618  MVHLGVLMHWFPPESSTETEEKVSMIEVHNRLL 520
            +++LGVLMHWFP ESST+ E K S+IE  NR+L
Sbjct: 545  VMNLGVLMHWFPNESSTQIEGKTSVIEA-NRML 576