BLASTX nr result
ID: Bupleurum21_contig00021757
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00021757
(1071 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002533596.1| conserved hypothetical protein [Ricinus comm... 302 8e-80
ref|XP_002278535.1| PREDICTED: DNA-directed RNA polymerase 2, ch... 299 7e-79
sp|Q8VWF8.2|RPOT2_NICSY RecName: Full=DNA-directed RNA polymeras... 289 9e-76
sp|Q8L6J3.2|RPO2B_TOBAC RecName: Full=DNA-directed RNA polymeras... 286 5e-75
dbj|BAB78464.1| T7 bacteriophage-type single subunit RNA polymer... 286 6e-75
>ref|XP_002533596.1| conserved hypothetical protein [Ricinus communis]
gi|223526525|gb|EEF28787.1| conserved hypothetical
protein [Ricinus communis]
Length = 395
Score = 302 bits (774), Expect = 8e-80
Identities = 160/267 (59%), Positives = 188/267 (70%), Gaps = 5/267 (1%)
Frame = +3
Query: 285 TDCEEGGN---EVDELLHELKKEDRRGYQ--RKRWRHYGGFKYQKLKKRQVKIETEAWVQ 449
TD EE + EV ELL E+++E+ R + R++ R G KYQ LKKRQVKIETEAW
Sbjct: 115 TDVEEDVSVVEEVQELLQEMRREEAREKELIRRKERGMGSKKYQMLKKRQVKIETEAWEW 174
Query: 450 AVREYRNLMTEMCDRNLAPNLPYMKSLFLGWFEPLTNKINEDQEVCRTGKTKAAYAPYFD 629
AV+EY+ L+ +MC++ LAPNLPYMKSLFLGWFEP + I ++QE+ R GK + A+APYFD
Sbjct: 175 AVKEYKELLMDMCEKKLAPNLPYMKSLFLGWFEPFRDAILKEQELIRVGKNRTAFAPYFD 234
Query: 630 QLPADMMAVITXXXXXXXXXXXXXXXXTRVVQAACAVGEAIEQEIRIHCFLEXXXXXXXX 809
QLPADMMAVIT RVV AAC +G AIEQE+RIH FLE
Sbjct: 235 QLPADMMAVITMHKLVALVMTGGEHGCARVVAAACLIGAAIEQEVRIHKFLEKTKKRNSN 294
Query: 810 XXXXNEEDPSDLVTKEQGKLRKTVTNLIKKQKLQTVRTIIKGQDTSKPWTPDVKAKVGSR 989
NEED S+ KEQ KLRK VT+LIKKQKL VR I+KG D S+ WT D KAKVGSR
Sbjct: 295 DSKKNEEDESNGAVKEQEKLRKKVTDLIKKQKLPAVRQIVKGHDDSQSWTQDTKAKVGSR 354
Query: 990 LIELLLQTAYIQPPADQLAEGPPDIRP 1070
LIELL+QTAYIQPPADQLA+GPPDIRP
Sbjct: 355 LIELLIQTAYIQPPADQLADGPPDIRP 381
>ref|XP_002278535.1| PREDICTED: DNA-directed RNA polymerase 2, chloroplastic/mitochondrial
[Vitis vinifera] gi|298204431|emb|CBI16911.3| unnamed
protein product [Vitis vinifera]
Length = 1035
Score = 299 bits (766), Expect = 7e-79
Identities = 172/342 (50%), Positives = 211/342 (61%), Gaps = 17/342 (4%)
Frame = +3
Query: 96 VKYDKFEPNEIITCPKNGLPKMGFDHMGHELLKDKI-----FSGING--GFNGLCXXXXX 254
+K+ FEP+ +C +G +MGF +G ++ F + GF+G C
Sbjct: 83 MKFGTFEPS---SCSSSGFSEMGFRRIGEISSREDAVGRPSFGCLRNQMGFSGFCPKSYA 139
Query: 255 XXXXXXXXXXTDCEE---GGNEVDELLHELKKEDRRGYQRKRW------RHYGGFKYQKL 407
TD EE +E+ ELL E+KKE++R KR R +Y +L
Sbjct: 140 SVAEAVSS--TDVEEDVSASDEIQELLGEMKKEEKRQVNFKRREKLKMERGMEQGRYLRL 197
Query: 408 KKRQVKIETEAWVQAVREYRNLMTEMCDRNLAPNLPYMKSLFLGWFEPLTNKINEDQEVC 587
++RQVKIETEAW A +EY+ L+ +MC++ LAPNLPYMKSLFLGWFEPL + I ++QE+C
Sbjct: 198 RRRQVKIETEAWELAAKEYKELLMDMCEQKLAPNLPYMKSLFLGWFEPLRDAIVKEQELC 257
Query: 588 RTGKTKAAYAPYFDQLPADMMAVITXXXXXXXXXXXXXXXXTRVVQAACAVGEAIEQEIR 767
R GK KAAYAPYFDQLPADMM+VIT TRVVQAAC +G+AIEQE+R
Sbjct: 258 RLGKNKAAYAPYFDQLPADMMSVITMHKLVGLLMTGGEHGSTRVVQAACVIGDAIEQEVR 317
Query: 768 IHCFLEXXXXXXXXXXXXNEEDPSDLVTKEQGKLRKTVTNLIKKQKLQTVRTIIKGQDTS 947
IH FLE E D V EQ KLRK VTNL+KKQKL R I+KG D S
Sbjct: 318 IHNFLEKTKRKKVGKDKNVEGGGFDPVIHEQEKLRKKVTNLMKKQKLHAARQIVKGHDNS 377
Query: 948 KPWTPDVKAKVGSRLIELLLQTAYIQPPAD-QLAEGPPDIRP 1070
KPW P+ KAKVGSRLIELL+QTAYIQPP D QLA+ PPDIRP
Sbjct: 378 KPWGPEAKAKVGSRLIELLMQTAYIQPPVDNQLADTPPDIRP 419
>sp|Q8VWF8.2|RPOT2_NICSY RecName: Full=DNA-directed RNA polymerase 2,
chloroplastic/mitochondrial; AltName: Full=NsRpoT-B;
AltName: Full=T7 bacteriophage-type single subunit RNA
polymerase 2; Flags: Precursor
gi|21425639|emb|CAC82575.2| phage-type RNA polymerase
[Nicotiana sylvestris]
Length = 1020
Score = 289 bits (739), Expect = 9e-76
Identities = 168/319 (52%), Positives = 200/319 (62%), Gaps = 15/319 (4%)
Frame = +3
Query: 159 MGFDHMGHELLKDKIFSG------INGGFNGLCXXXXXXXXXXXXXXXTDCEEGGNEVDE 320
MGF ++G L D+ S +N F C TD EE + VDE
Sbjct: 90 MGFQNLGEYLPGDEFLSRPLIKNQVNNNF--CCRKSYASVAEAVAVSSTDAEEDVSVVDE 147
Query: 321 ---LLHELKKED------RRGYQRKRWRHYGGFKYQKLKKRQVKIETEAWVQAVREYRNL 473
LL ELKKE+ RR QR G KYQ LK+RQVK+ETEAW QA +EY+ L
Sbjct: 148 VHELLTELKKEEKKQFAFRRRKQRMLTSGMGHRKYQTLKRRQVKVETEAWEQAAKEYKEL 207
Query: 474 MTEMCDRNLAPNLPYMKSLFLGWFEPLTNKINEDQEVCRTGKTKAAYAPYFDQLPADMMA 653
+ +MC++ LAPNLPY+KSLFLGWFEPL +KI E+QE+C GK+KAAYA YF QLPADMMA
Sbjct: 208 LFDMCEQKLAPNLPYVKSLFLGWFEPLRDKIAEEQELCSQGKSKAAYAKYFYQLPADMMA 267
Query: 654 VITXXXXXXXXXXXXXXXXTRVVQAACAVGEAIEQEIRIHCFLEXXXXXXXXXXXXNEED 833
VIT RVVQAA +G+AIEQE+RIH FLE E+
Sbjct: 268 VITMHKLMGLLMTGGDHGTARVVQAALVIGDAIEQEVRIHNFLEKTKKQKAEKDKQKED- 326
Query: 834 PSDLVTKEQGKLRKTVTNLIKKQKLQTVRTIIKGQDTSKPWTPDVKAKVGSRLIELLLQT 1013
+ VT+EQ KLRK VTNL+KKQKL+ V I++ QD SKPW D +AKVGSRLI+LLLQT
Sbjct: 327 -GEHVTQEQEKLRKKVTNLMKKQKLRAVGQIVRRQDDSKPWGQDARAKVGSRLIDLLLQT 385
Query: 1014 AYIQPPADQLAEGPPDIRP 1070
AYIQPPA+QLA PPDIRP
Sbjct: 386 AYIQPPANQLAVDPPDIRP 404
>sp|Q8L6J3.2|RPO2B_TOBAC RecName: Full=DNA-directed RNA polymerase 2B,
chloroplastic/mitochondrial; AltName:
Full=NictaRpoT2-tom; AltName: Full=T7 bacteriophage-type
single subunit RNA polymerase 2B; Flags: Precursor
gi|21425665|emb|CAC95020.1| mitochondrial and chloroplast
RNA polymerase [Nicotiana tabacum]
Length = 1021
Score = 286 bits (733), Expect = 5e-75
Identities = 168/319 (52%), Positives = 199/319 (62%), Gaps = 15/319 (4%)
Frame = +3
Query: 159 MGFDHMGHELLKDKIFSG------INGGFNGLCXXXXXXXXXXXXXXXTDCEEGGN---E 311
MGF ++G L D+ S +N + C TD EE + E
Sbjct: 90 MGFQNLGEYLPGDEFLSRPLLKNQVNSN-DFCCRKSYASVAEAVAVSSTDAEEDVSVVDE 148
Query: 312 VDELLHELKKED------RRGYQRKRWRHYGGFKYQKLKKRQVKIETEAWVQAVREYRNL 473
V ELL ELKKE+ RR QR G KYQ LK+RQVK+ETEAW QA +EY+ L
Sbjct: 149 VQELLTELKKEEKKQFAFRRRKQRMLTSGMGHRKYQTLKRRQVKVETEAWEQAAKEYKEL 208
Query: 474 MTEMCDRNLAPNLPYMKSLFLGWFEPLTNKINEDQEVCRTGKTKAAYAPYFDQLPADMMA 653
+ +MC++ LAPNLPY+KSLFLGWFEPL +KI E+QE+C GK+KAAYA Y QLPADMMA
Sbjct: 209 LFDMCEQKLAPNLPYVKSLFLGWFEPLRDKIAEEQELCSQGKSKAAYAKYLYQLPADMMA 268
Query: 654 VITXXXXXXXXXXXXXXXXTRVVQAACAVGEAIEQEIRIHCFLEXXXXXXXXXXXXNEED 833
VIT RVVQAA +G+AIEQE+RIH FLE E+
Sbjct: 269 VITMHKLMGLLMTGGDHGTARVVQAALVIGDAIEQEVRIHNFLEKTKKQKAEKDKQKED- 327
Query: 834 PSDLVTKEQGKLRKTVTNLIKKQKLQTVRTIIKGQDTSKPWTPDVKAKVGSRLIELLLQT 1013
+ VT+EQ KLRK VTNL+KKQKL+ V I++ QD SKPW D KAKVGSRLIELLLQT
Sbjct: 328 -GEHVTQEQEKLRKKVTNLMKKQKLRAVGQIVRRQDDSKPWGQDAKAKVGSRLIELLLQT 386
Query: 1014 AYIQPPADQLAEGPPDIRP 1070
AYIQPPA+QLA PPDIRP
Sbjct: 387 AYIQPPANQLAVDPPDIRP 405
>dbj|BAB78464.1| T7 bacteriophage-type single subunit RNA polymerase [Nicotiana
sylvestris] gi|17221595|dbj|BAB78465.1| T7
bacteriophage-type single subunit RNA polymerase
[Nicotiana sylvestris]
Length = 1020
Score = 286 bits (732), Expect = 6e-75
Identities = 167/319 (52%), Positives = 199/319 (62%), Gaps = 15/319 (4%)
Frame = +3
Query: 159 MGFDHMGHELLKDKIFSG------INGGFNGLCXXXXXXXXXXXXXXXTDCEEGGNEVDE 320
MGF ++G L D+ S +N F C TD EE + VDE
Sbjct: 90 MGFQNLGEYLPGDEFLSRPLIKNQVNNNF--CCRKSYASVAEAVAVSSTDAEEDVSVVDE 147
Query: 321 ---LLHELKKED------RRGYQRKRWRHYGGFKYQKLKKRQVKIETEAWVQAVREYRNL 473
LL ELKKE+ RR QR G KYQ LK+RQVK+ETEAW QA +EY+ L
Sbjct: 148 VHELLTELKKEEKKQFAFRRRKQRMLTSGMGHRKYQTLKRRQVKVETEAWEQAAKEYKEL 207
Query: 474 MTEMCDRNLAPNLPYMKSLFLGWFEPLTNKINEDQEVCRTGKTKAAYAPYFDQLPADMMA 653
+ +MC++ LAPNLPY+KSLFLGWFEPL +KI E+QE+C GK+KAAYA YF QLPADMMA
Sbjct: 208 LFDMCEQKLAPNLPYVKSLFLGWFEPLRDKIAEEQELCSQGKSKAAYAKYFYQLPADMMA 267
Query: 654 VITXXXXXXXXXXXXXXXXTRVVQAACAVGEAIEQEIRIHCFLEXXXXXXXXXXXXNEED 833
VIT RVVQAA +G+AIEQE+RIH FLE E+
Sbjct: 268 VITMHKLMGLLMTGGDHGTARVVQAALVIGDAIEQEVRIHNFLEKTKKQKAEKDKQKED- 326
Query: 834 PSDLVTKEQGKLRKTVTNLIKKQKLQTVRTIIKGQDTSKPWTPDVKAKVGSRLIELLLQT 1013
+ VT+EQ KLRK VTNL+KKQKL+ V I++ QD SKPW D +AKVGSRLI+LLLQT
Sbjct: 327 -GEHVTQEQEKLRKKVTNLMKKQKLRAVGQIVRRQDDSKPWGQDARAKVGSRLIDLLLQT 385
Query: 1014 AYIQPPADQLAEGPPDIRP 1070
AYIQPPA+QLA PPDI P
Sbjct: 386 AYIQPPANQLAVDPPDIPP 404