BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00021722
         (1234 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002312933.1| flavonoid o-methyltransferase predicted prot...   373   e-101
ref|XP_002281120.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran...   372   e-101
ref|XP_002529795.1| o-methyltransferase, putative [Ricinus commu...   369   1e-99
ref|XP_002281110.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltran...   366   6e-99
gb|AFK49425.1| unknown [Lotus japonicus]                              359   7e-97

>ref|XP_002312933.1| flavonoid o-methyltransferase predicted protein [Populus trichocarpa]
            gi|222849341|gb|EEE86888.1| flavonoid o-methyltransferase
            predicted protein [Populus trichocarpa]
          Length = 356

 Score =  373 bits (958), Expect = e-101
 Identities = 188/358 (52%), Positives = 251/358 (70%), Gaps = 6/358 (1%)
 Frame = -3

Query: 1196 MSEIAKKEATKEYDDETARAQVDIWKYVFGFSETAVVKCAVELGIPDILENHGGPMTXXX 1017
            M  + K +     D+E A+A+V+IWKY+FGF+  AVVKCA+ELGI D +EN+ GPMT   
Sbjct: 1    MGSVTKIDEEIREDEEEAQAKVEIWKYIFGFTNMAVVKCAIELGIADAIENNEGPMTLSE 60

Query: 1016 XXXXXXXXXXXLFRIMRFLMNRGIFKET------TMGYAQTPLSRLLIKDGKDSMAAVML 855
                       L+RIMRFL++  IFKE       T  Y QT LSR L+K G+ SM  ++L
Sbjct: 61   LSSSLGCAPSSLYRIMRFLVHHNIFKEKPSSQLGTTVYVQTALSRRLLKKGEKSMVDLLL 120

Query: 854  FLSSSVLTAPLHFLSASVLDEKTSPFVRAYGKNMWQYAAESPGFCKLVDESMACGSRKTV 675
              SS V+ AP H LS+ VL++  SPF  A+G ++W+YA  +P   KL+D++MAC ++  V
Sbjct: 121  LESSHVMMAPWHNLSSRVLNDNNSPFEGAHGDDIWKYALANPVHSKLIDDAMACDAKLVV 180

Query: 674  PAVLEGCPEVFDGLSSVVNVGGGDGTALRMIIKECPWISGINFDLPQVLSLAPTCEGIEH 495
            P ++EG PEVFDG+ ++V+VGGG+GT L+M++K  PWI GINFDLP V+S+A   EG++H
Sbjct: 181  PEIVEGFPEVFDGVKTLVDVGGGNGTTLQMLVKAFPWIQGINFDLPHVVSVASESEGVKH 240

Query: 494  ISGDMFVSVPKANAAFLKLILHDWNDEKCIQILKNCREAISEYGTTGKVIIVEAIVEDKP 315
            + GD F SVPKA+AAFL  +LHDWNDE+CIQILKNC+EAI      GKVIIVEA+V ++ 
Sbjct: 241  VGGDFFESVPKADAAFLMWVLHDWNDEECIQILKNCKEAIQ--SDKGKVIIVEAVVGEEK 298

Query: 314  GDKLKDVGLFFDMLMFANTDSGKERTADEWAYILSEGGFTRHTIKDIDGVLSVIEAYP 141
            GDKL+ V L  DM+M ++TD+GKERT+ EW Y+L E GF+ +TIK I  V SVI A P
Sbjct: 299  GDKLEFVRLMLDMVMMSHTDAGKERTSKEWGYVLKEAGFSSYTIKPIRAVQSVIVASP 356


>ref|XP_002281120.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase [Vitis vinifera]
          Length = 353

 Score =  372 bits (956), Expect = e-101
 Identities = 191/354 (53%), Positives = 250/354 (70%), Gaps = 8/354 (2%)
 Frame = -3

Query: 1178 KEATKEYDDETARAQVDIWKYVFGFSETAVVKCAVELGIPDILENHGGPMTXXXXXXXXX 999
            ++  K  + E A A+V++WKY+FGF E AVVKC +ELGI D++E+H GP+T         
Sbjct: 2    EKVVKIMEKEEAEAEVEMWKYIFGFVEMAVVKCGIELGIADVIESHAGPITLSSLSSSLG 61

Query: 998  XXXXXLFRIMRFLMNRGIFKETTM-----GYAQTPLSRLLIKDGKDSMAAVMLFLSSSVL 834
                 L+RIMRFL+NR IFKE        GY QTPLSR L+   ++ MAA++L  SS V+
Sbjct: 62   CSPSGLYRIMRFLVNRRIFKEVATSQGDTGYQQTPLSRRLMTRSENGMAALLLLESSPVM 121

Query: 833  TAPLHFLSASVLDEKTSPFVRAYGKNMWQYAAESPGFCKLVDESMACGSRKTVPAVLEGC 654
             AP H LSA +L +  + F  A+G+++W YAA  P   KL++++MAC +R  V A++ GC
Sbjct: 122  LAPWHGLSARLLGKGNATFDAAHGQDVWGYAASHPAHSKLINDAMACDARMAVSAIVNGC 181

Query: 653  PEVFDGLSSVVNVGGGDGTALRMIIKECPWISGINFDLPQVLSLAPTCEGIEHISGDMFV 474
            PEVFDG+S++V+VGGGDGTALR +IK  P I GINFDLP V+S AP C G+E+ SGDMF 
Sbjct: 182  PEVFDGVSTLVDVGGGDGTALRTLIKARPLIRGINFDLPHVVSSAPKCNGVEYASGDMFD 241

Query: 473  SVPKANAAFLKLILHDWNDEKCIQILKNCREAISEYGTTGKVIIVEAIVE--DKPGD-KL 303
            +VPKA+AAFL  +LHDW DE+CIQIL+ CR+AI   G  GKVIIVEA+++  +K GD  L
Sbjct: 242  TVPKADAAFLMWVLHDWGDEECIQILEKCRQAIP--GDKGKVIIVEAVIQENEKEGDNNL 299

Query: 302  KDVGLFFDMLMFANTDSGKERTADEWAYILSEGGFTRHTIKDIDGVLSVIEAYP 141
            KDVGL  DM+M A+T +GKERT  EW Y+L + GF R+T+K I  V SVIEAYP
Sbjct: 300  KDVGLMLDMVMMAHTTTGKERTLKEWDYVLKKAGFNRYTVKPIRAVKSVIEAYP 353


>ref|XP_002529795.1| o-methyltransferase, putative [Ricinus communis]
            gi|223530706|gb|EEF32577.1| o-methyltransferase, putative
            [Ricinus communis]
          Length = 345

 Score =  369 bits (946), Expect = 1e-99
 Identities = 182/345 (52%), Positives = 244/345 (70%), Gaps = 6/345 (1%)
 Frame = -3

Query: 1157 DDETARAQVDIWKYVFGFSETAVVKCAVELGIPDILENHGGPMTXXXXXXXXXXXXXXLF 978
            ++E A+A+ ++WKYVFGF+  AVVKCA+ELGI D +EN   P+T              L+
Sbjct: 3    EEEEAQAEAEMWKYVFGFTNMAVVKCAIELGIADAIENRKEPVTLSELSSTLGCAPSSLY 62

Query: 977  RIMRFLMNRGIFKE------TTMGYAQTPLSRLLIKDGKDSMAAVMLFLSSSVLTAPLHF 816
            RIMRFLM++ IFKE      T++GY  TPLSR L+  G+D+MAA +L  SS V+ AP HF
Sbjct: 63   RIMRFLMHQNIFKEIPTHQGTSIGYVHTPLSRRLLGRGEDNMAAFLLLESSPVMLAPWHF 122

Query: 815  LSASVLDEKTSPFVRAYGKNMWQYAAESPGFCKLVDESMACGSRKTVPAVLEGCPEVFDG 636
            LSA V     + F   +G ++W+YAA +PG  KL+D++MAC +R  V  ++E CP+VFDG
Sbjct: 123  LSARVQQNGRAAFEAVHGDDVWKYAAANPGHSKLIDDAMACNARTAVTTIIEQCPKVFDG 182

Query: 635  LSSVVNVGGGDGTALRMIIKECPWISGINFDLPQVLSLAPTCEGIEHISGDMFVSVPKAN 456
            + ++V+VGGG+GTALRM++K  PWI GINFDL  V+S+A  C  + +I GDMF SVPKA+
Sbjct: 183  IKTLVDVGGGNGTALRMLVKAFPWIQGINFDLHHVVSIAHECNNVTYIGGDMFESVPKAD 242

Query: 455  AAFLKLILHDWNDEKCIQILKNCREAISEYGTTGKVIIVEAIVEDKPGDKLKDVGLFFDM 276
            AAFL  +LHDWND+ CIQILK C+EA++E    GKVIIVEA++ +   DKL+ V L  DM
Sbjct: 243  AAFLMWVLHDWNDDDCIQILKKCKEAVTE--GNGKVIIVEAVIGEAKDDKLEYVRLMLDM 300

Query: 275  LMFANTDSGKERTADEWAYILSEGGFTRHTIKDIDGVLSVIEAYP 141
            +M A+T++GKERT+ EW Y+L + GF  HTIK I  V SVIEA+P
Sbjct: 301  VMMAHTNTGKERTSKEWGYVLQKAGFRSHTIKPIGAVQSVIEAFP 345


>ref|XP_002281110.1| PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Vitis
            vinifera]
          Length = 359

 Score =  366 bits (940), Expect = 6e-99
 Identities = 188/362 (51%), Positives = 252/362 (69%), Gaps = 10/362 (2%)
 Frame = -3

Query: 1196 MSEIAKKEATKEYDDETARAQVDIWKYVFGFSETAVVKCAVELGIPDILENHGGPMTXXX 1017
            M+E  +   T E ++E A   +++WKY++GF+E A VKC +ELGI D++E+HGGP+T   
Sbjct: 1    MTEATRDMKTLE-EEEEAEVGIEMWKYIYGFAEMAAVKCGIELGIADVMESHGGPITLSA 59

Query: 1016 XXXXXXXXXXXLFRIMRFLMNRGIFKETTM-----GYAQTPLSRLLIKDGKDSMAAVMLF 852
                       L RIMRFL++R IFKE        GY QTP+SR L+  G D MAA +L 
Sbjct: 60   LSSSLGCPPSGLNRIMRFLVSRRIFKEVATSQGDTGYQQTPMSRCLMTSGGDGMAAFVLL 119

Query: 851  LSSSVLTAPLHFLSASVLDEKTSPFVRAYGKNMWQYAAESPGFCKLVDESMACGSRKTVP 672
             SS V+ AP H LSA VL +  S F  A+G ++W YAA++P   KL++++MA  +R+ VP
Sbjct: 120  ESSPVMLAPWHGLSARVLGKGNSTFEAAHGDDIWGYAAKNPDHSKLINDAMASNARRVVP 179

Query: 671  AVLEGCPEVFDGLSSVVNVGGGDGTALRMIIKECPWISGINFDLPQVLSLAPTCEGIEHI 492
            A++  CPEVFDG+S++V+VGGG+GTALR ++K CPWI GINFDLP V++ AP C  +EH+
Sbjct: 180  AMVNDCPEVFDGVSTLVDVGGGNGTALRTLVKACPWIRGINFDLPHVVATAPKCNDVEHV 239

Query: 491  SGDMFVSVPKANAAFLKLILHDWNDEKCIQILKNCREAISEYGTTGKVIIVEAIVE--DK 318
             G+MF SVPKA+A F+  +LHDW DEKCI+IL+ CREAI E    GKV+IVEA++E  D 
Sbjct: 240  GGNMFDSVPKADAVFIMKVLHDWGDEKCIKILEKCREAIPE--DKGKVVIVEAVIEDGDP 297

Query: 317  PGD--KLKDVGLFFDMLMFANTDSGKERTADEWAYILSEGGFTRHTIKDIDGVL-SVIEA 147
             GD  KLKDV L  DM+M A+T +GKERT  EW Y+L   GF+R+TIK I  ++ S+IEA
Sbjct: 298  QGDDQKLKDVKLMVDMVMMAHTTTGKERTFKEWDYVLLNAGFSRYTIKPIRNIVQSIIEA 357

Query: 146  YP 141
            YP
Sbjct: 358  YP 359


>gb|AFK49425.1| unknown [Lotus japonicus]
          Length = 366

 Score =  359 bits (922), Expect = 7e-97
 Identities = 181/361 (50%), Positives = 251/361 (69%), Gaps = 11/361 (3%)
 Frame = -3

Query: 1190 EIAKKEATKEYDDETARAQVDIWKYVFGFSETAVVKCAVELGIPDILENHGGPMTXXXXX 1011
            ++++ E  +E ++E A+AQ+DIWKY+FGF E AVVKCA+ELGI + +E HG PMT     
Sbjct: 8    KLSQWEKEQEEEEEEAQAQIDIWKYIFGFVEQAVVKCAIELGIAEAIEAHGKPMTLSELS 67

Query: 1010 XXXXXXXXXLFRIMRFLMNRGIFKETT--------MGYAQTPLSRLLIKDGKDSMAAVML 855
                     L RIMRFL+ R IFK TT        + Y Q+PLSR L+++G+ SM A+++
Sbjct: 68   STLRCDPSYLNRIMRFLVRRKIFKTTTSTTNHGCDLSYVQSPLSRRLLRNGEHSMTALLM 127

Query: 854  FLSSSVLTAPLHFLSASVLDEKTSPFVRAYGKNMWQYAAESPGFCKLVDESMACGSRKTV 675
              SS V+ AP H LS  VL +    F + +G+++W YAA +     L++E+MAC +   V
Sbjct: 128  LESSHVMLAPWHGLSDRVLVDGNPSFKKVHGEDVWDYAATNLEHSNLINEAMACDANLVV 187

Query: 674  PAVLEGCPEVFDGLSSVVNVGGGDGTALRMIIKECPWISGINFDLPQVL-SLAPTCEGIE 498
            P ++EGC EVF+GL+++V+VGGG+G A+ ++ K CPWI  INFDLP V+ ++AP C G+E
Sbjct: 188  PVMVEGCSEVFEGLTTLVDVGGGNGAAMGVVAKSCPWIKAINFDLPHVIAAVAPKCNGVE 247

Query: 497  HISGDMFVSVPKANAAFLKLILHDWNDEKCIQILKNCREAISEYGTTGKVIIVEAIVEDK 318
             ++GDMF SVPKA+A F+K +LHDW DE+CIQILKNCREAI +    G+VIIVEA++E++
Sbjct: 248  FVAGDMFASVPKADAVFIKWVLHDWGDEECIQILKNCREAIPK--ENGRVIIVEAVIEEE 305

Query: 317  PG--DKLKDVGLFFDMLMFANTDSGKERTADEWAYILSEGGFTRHTIKDIDGVLSVIEAY 144
             G  DKLKDVGL  DM+M A+T+ GKERT  EW Y++   GF+  T+K I+ V SVI A 
Sbjct: 306  EGKHDKLKDVGLMLDMVMMAHTNFGKERTLKEWEYVIKMAGFSTFTVKAINAVQSVILAL 365

Query: 143  P 141
            P
Sbjct: 366  P 366