BLASTX nr result
ID: Bupleurum21_contig00021643
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00021643
(1558 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002270987.2| PREDICTED: proline-rich receptor-like protei... 424 e-116
emb|CBI28844.3| unnamed protein product [Vitis vinifera] 424 e-116
ref|XP_002317145.1| predicted protein [Populus trichocarpa] gi|2... 420 e-115
emb|CBI23197.3| unnamed protein product [Vitis vinifera] 412 e-112
gb|AEO18237.1| Bti9 [Nicotiana benthamiana] 410 e-112
>ref|XP_002270987.2| PREDICTED: proline-rich receptor-like protein kinase PERK13-like
[Vitis vinifera]
Length = 619
Score = 424 bits (1089), Expect = e-116
Identities = 222/350 (63%), Positives = 259/350 (74%), Gaps = 4/350 (1%)
Frame = -2
Query: 1380 VTAGLSIAGVIGALSLAACFYFGFYXXXXXXXXXXXKTSYHDHAMGNKFASGLNLEKNAD 1201
V AG+SIA V+G L L C Y GFY T H G+ G+ +K +
Sbjct: 229 VIAGISIAAVVGVLLLTVCIYIGFYRKRKVKEAALLPTEEHSLQPGH--GPGIASDKAVE 286
Query: 1200 SGHLNDGVFSELTDISMDKSLEFSYKELAEATDNFSLAKKIGQGGFGAVYYAELRGEKAA 1021
S G + LT I++DKS+EFSY+ELA+A+DNF+LA KIGQGGFG+VYYAELRGEKAA
Sbjct: 287 STGPAFGSSAGLTGITVDKSVEFSYEELAKASDNFNLANKIGQGGFGSVYYAELRGEKAA 346
Query: 1020 VKKMDMHATKAFLAELKVLTHVHHLNLVRLIGYSVESSLLIVYEYIDNGNLCQHLRGLSG 841
+KKMDM A++ FLAELKVLTHVHHLNLVRLIGY VE SL +VYEYI+NGNL QHLRG SG
Sbjct: 347 IKKMDMQASREFLAELKVLTHVHHLNLVRLIGYCVEGSLFLVYEYIENGNLSQHLRG-SG 405
Query: 840 KGPLSWSSRLQISLDSARGLEYIHEHTNPAYIHRDIKSPNILLDKQLRAKVADFGLTKLV 661
+ PL WSSR+QI+LDSARGLEYIHEHT P YIHRDIKS NIL+DK KVADFGLTKL
Sbjct: 406 RDPLQWSSRVQIALDSARGLEYIHEHTVPVYIHRDIKSANILIDKNFHGKVADFGLTKLT 465
Query: 660 EVGTGSLQTRLVGTFGYMPPEYAQYGEVSTKIDVYAFGVLLYELISAREAIGNSKDVSTR 481
EVG+ SL TRLVGTFGYMPPEYAQYG+VS K+DVYAFGV+LYELISA+EA+
Sbjct: 466 EVGSSSLPTRLVGTFGYMPPEYAQYGDVSPKVDVYAFGVVLYELISAKEAVVKDNGSVAE 525
Query: 480 TMGLVTLFEEVMNLPDPTTDLRKLVDPSF*DSPACESLYK----PKPCTE 343
+ GLV LFE+V+N PDP DLRKLVDP D+ +S+ K K CT+
Sbjct: 526 SKGLVALFEDVLNKPDPREDLRKLVDPRLEDNYPLDSVRKMAQLAKACTQ 575
Score = 86.7 bits (213), Expect = 2e-14
Identities = 45/68 (66%), Positives = 54/68 (79%), Gaps = 1/68 (1%)
Frame = -3
Query: 428 PRISANW-LIPVFRIAQLAKACTNPNPVLRPSMRAVVVALMTISSGTENWYVGSLDENQS 252
PR+ N+ L V ++AQLAKACT NP LRPSMR +VVALMT+SS TE+W VGS +NQ+
Sbjct: 552 PRLEDNYPLDSVRKMAQLAKACTQENPQLRPSMRTIVVALMTLSSSTEDWDVGSFYDNQA 611
Query: 251 LVNLMSGR 228
LVNLMSGR
Sbjct: 612 LVNLMSGR 619
>emb|CBI28844.3| unnamed protein product [Vitis vinifera]
Length = 625
Score = 424 bits (1089), Expect = e-116
Identities = 222/350 (63%), Positives = 259/350 (74%), Gaps = 4/350 (1%)
Frame = -2
Query: 1380 VTAGLSIAGVIGALSLAACFYFGFYXXXXXXXXXXXKTSYHDHAMGNKFASGLNLEKNAD 1201
V AG+SIA V+G L L C Y GFY T H G+ G+ +K +
Sbjct: 235 VIAGISIAAVVGVLLLTVCIYIGFYRKRKVKEAALLPTEEHSLQPGH--GPGIASDKAVE 292
Query: 1200 SGHLNDGVFSELTDISMDKSLEFSYKELAEATDNFSLAKKIGQGGFGAVYYAELRGEKAA 1021
S G + LT I++DKS+EFSY+ELA+A+DNF+LA KIGQGGFG+VYYAELRGEKAA
Sbjct: 293 STGPAFGSSAGLTGITVDKSVEFSYEELAKASDNFNLANKIGQGGFGSVYYAELRGEKAA 352
Query: 1020 VKKMDMHATKAFLAELKVLTHVHHLNLVRLIGYSVESSLLIVYEYIDNGNLCQHLRGLSG 841
+KKMDM A++ FLAELKVLTHVHHLNLVRLIGY VE SL +VYEYI+NGNL QHLRG SG
Sbjct: 353 IKKMDMQASREFLAELKVLTHVHHLNLVRLIGYCVEGSLFLVYEYIENGNLSQHLRG-SG 411
Query: 840 KGPLSWSSRLQISLDSARGLEYIHEHTNPAYIHRDIKSPNILLDKQLRAKVADFGLTKLV 661
+ PL WSSR+QI+LDSARGLEYIHEHT P YIHRDIKS NIL+DK KVADFGLTKL
Sbjct: 412 RDPLQWSSRVQIALDSARGLEYIHEHTVPVYIHRDIKSANILIDKNFHGKVADFGLTKLT 471
Query: 660 EVGTGSLQTRLVGTFGYMPPEYAQYGEVSTKIDVYAFGVLLYELISAREAIGNSKDVSTR 481
EVG+ SL TRLVGTFGYMPPEYAQYG+VS K+DVYAFGV+LYELISA+EA+
Sbjct: 472 EVGSSSLPTRLVGTFGYMPPEYAQYGDVSPKVDVYAFGVVLYELISAKEAVVKDNGSVAE 531
Query: 480 TMGLVTLFEEVMNLPDPTTDLRKLVDPSF*DSPACESLYK----PKPCTE 343
+ GLV LFE+V+N PDP DLRKLVDP D+ +S+ K K CT+
Sbjct: 532 SKGLVALFEDVLNKPDPREDLRKLVDPRLEDNYPLDSVRKMAQLAKACTQ 581
Score = 86.7 bits (213), Expect = 2e-14
Identities = 45/68 (66%), Positives = 54/68 (79%), Gaps = 1/68 (1%)
Frame = -3
Query: 428 PRISANW-LIPVFRIAQLAKACTNPNPVLRPSMRAVVVALMTISSGTENWYVGSLDENQS 252
PR+ N+ L V ++AQLAKACT NP LRPSMR +VVALMT+SS TE+W VGS +NQ+
Sbjct: 558 PRLEDNYPLDSVRKMAQLAKACTQENPQLRPSMRTIVVALMTLSSSTEDWDVGSFYDNQA 617
Query: 251 LVNLMSGR 228
LVNLMSGR
Sbjct: 618 LVNLMSGR 625
>ref|XP_002317145.1| predicted protein [Populus trichocarpa] gi|222860210|gb|EEE97757.1|
predicted protein [Populus trichocarpa]
Length = 628
Score = 420 bits (1079), Expect = e-115
Identities = 221/376 (58%), Positives = 272/376 (72%), Gaps = 10/376 (2%)
Frame = -2
Query: 1440 DQTRSYPP------FXXXXXXXXXXGVTAGLSIAGVIGALSLAACFYFGFYXXXXXXXXX 1279
D T +YPP + AG+S+AG+ GA LA+CFYFGFY
Sbjct: 212 DPTGNYPPLKILCCYAWPFQAGISSRAIAGISVAGIAGAFFLASCFYFGFYRRREVEASL 271
Query: 1278 XXKTSYHDHAMGNKFASGLNLEKNADSGHLNDGVFSELTDISMDKSLEFSYKELAEATDN 1099
+ + + + ++ SG LE+ +++ L LT ++DKS+EFSY+ELA+AT++
Sbjct: 272 FPEAAESPY-IHHRHGSGNILEQTSETAALVGS--PGLTGFTVDKSVEFSYEELAKATND 328
Query: 1098 FSLAKKIGQGGFGAVYYAELRGEKAAVKKMDMHATKAFLAELKVLTHVHHLNLVRLIGYS 919
FS+ KIGQGGFGAVYYAELRGEKAA+KKMDM A+K FLAELKVLTHVHHLNLVRLIGY
Sbjct: 329 FSMDNKIGQGGFGAVYYAELRGEKAAIKKMDMQASKEFLAELKVLTHVHHLNLVRLIGYC 388
Query: 918 VESSLLIVYEYIDNGNLCQHLRGLSGKGPLSWSSRLQISLDSARGLEYIHEHTNPAYIHR 739
VE SL +VYE+I+NGNL QHLRG SGK PL WS+R+Q++LDSARGLEYIHEHT P YIHR
Sbjct: 389 VEGSLFLVYEFIENGNLGQHLRGNSGKDPLPWSTRVQVALDSARGLEYIHEHTVPVYIHR 448
Query: 738 DIKSPNILLDKQLRAKVADFGLTKLVEVGTGSLQTRLVGTFGYMPPEYAQYGEVSTKIDV 559
D+KS NIL+DK R KVADFGLT+L EVG+ SL TRLVGTFGYMPPEYAQYG+VS+KIDV
Sbjct: 449 DVKSANILIDKNFRGKVADFGLTRLTEVGSASLHTRLVGTFGYMPPEYAQYGDVSSKIDV 508
Query: 558 YAFGVLLYELISAREAIGNSKDVSTRTMGLVTLFEEVMNLPDPTTDLRKLVDPSF*DSPA 379
YAFGV+LYELISA+EA+ + + T +MGLV LFEEV+ PDP +L KLVD D
Sbjct: 509 YAFGVVLYELISAKEAVVKTNEFITESMGLVALFEEVLGQPDPRENLPKLVDARLGDDYP 568
Query: 378 CESLYK----PKPCTE 343
+S+ K + CT+
Sbjct: 569 LDSVCKMAQLARACTQ 584
Score = 81.6 bits (200), Expect = 5e-13
Identities = 39/57 (68%), Positives = 50/57 (87%)
Frame = -3
Query: 398 VFRIAQLAKACTNPNPVLRPSMRAVVVALMTISSGTENWYVGSLDENQSLVNLMSGR 228
V ++AQLA+ACT NP +RPSMR++VVALMT+SS TE+W VGSL ENQ++V+LMSGR
Sbjct: 572 VCKMAQLARACTQENPHVRPSMRSIVVALMTLSSSTEDWDVGSLYENQAIVDLMSGR 628
>emb|CBI23197.3| unnamed protein product [Vitis vinifera]
Length = 650
Score = 412 bits (1060), Expect = e-112
Identities = 220/371 (59%), Positives = 263/371 (70%), Gaps = 5/371 (1%)
Frame = -2
Query: 1440 DQTRSYPPFXXXXXXXXXXGVTAGLSIAGVIGALSLAACFYFGFYXXXXXXXXXXXKTSY 1261
DQ +YPP GV AG+S+AG++G+L A + +
Sbjct: 237 DQNETYPPLKLSNSAGISSGVIAGISVAGIVGSLLFAFFLFARICKRKKVKKVLFFPAAS 296
Query: 1260 HDHAMGNKFASGLNLEKNADSGHLNDGVFSELTDISMDKSLEFSYKELAEATDNFSLAKK 1081
M ++ A G E+ +DS L L I++DKS+EFSY+ELA ATDNFSLA K
Sbjct: 297 EQQYMQHRQAHGSASEETSDSAALVGAASLGLVGITVDKSVEFSYEELATATDNFSLANK 356
Query: 1080 IGQGGFGAVYYAELRGEKAAVKKMDMHATKAFLAELKVLTHVHHLNLVRLIGYSVESSLL 901
IGQGGFG+VYYAELRGEKAA+KKMDM A+K FLAELKVLTHVHHLNLVRLIGY VE SL
Sbjct: 357 IGQGGFGSVYYAELRGEKAAIKKMDMQASKEFLAELKVLTHVHHLNLVRLIGYCVEGSLF 416
Query: 900 IVYEYIDNGNLCQHLRGLSGKGPLSWSSRLQISLDSARGLEYIHEHTNPAYIHRDIKSPN 721
+VYE+IDNGNL HLRG SGK PL WSSR+QI+LDSARGLEYIHEHT P YIHRDIK N
Sbjct: 417 LVYEFIDNGNLSHHLRG-SGKDPLPWSSRVQIALDSARGLEYIHEHTVPVYIHRDIKPAN 475
Query: 720 ILLDKQLRAKVADFGLTKLVEVGTGSLQTRLVGTFGYMPPEYAQYGEVSTKIDVYAFGVL 541
IL+DK+ RAKVADFGLTKL EVG+ S+ TRLVGTFGYMPPEYAQYG+VS KIDV+AFGV+
Sbjct: 476 ILIDKKFRAKVADFGLTKLTEVGSASIPTRLVGTFGYMPPEYAQYGDVSPKIDVFAFGVV 535
Query: 540 LYELISAREAIGNSKD-VSTRTMGLVTLFEEVMNLPDPTTDLRKLVDPSF*DSPACESLY 364
LYELISA+EAI + + + + GLV LFE+V++ PDP D KL+D D +S++
Sbjct: 536 LYELISAKEAIVKTNEPIMPESKGLVALFEDVLSQPDPREDFVKLIDQRLGDDYPLDSIW 595
Query: 363 K----PKPCTE 343
K K CT+
Sbjct: 596 KMAHLAKACTQ 606
Score = 82.0 bits (201), Expect = 4e-13
Identities = 38/57 (66%), Positives = 49/57 (85%)
Frame = -3
Query: 398 VFRIAQLAKACTNPNPVLRPSMRAVVVALMTISSGTENWYVGSLDENQSLVNLMSGR 228
++++A LAKACT NP LRPSMR++VVALMT+SS TE+W VGS EN++L+NLMSGR
Sbjct: 594 IWKMAHLAKACTQENPQLRPSMRSIVVALMTLSSSTEDWDVGSFYENEALMNLMSGR 650
>gb|AEO18237.1| Bti9 [Nicotiana benthamiana]
Length = 623
Score = 410 bits (1055), Expect = e-112
Identities = 220/370 (59%), Positives = 267/370 (72%), Gaps = 5/370 (1%)
Frame = -2
Query: 1440 DQTRSYPPFXXXXXXXXXXGVTAGLSIAGVIGALSLAACFYFGFYXXXXXXXXXXXKTS- 1264
D++ ++PP AG+SI + L LA Y G+Y
Sbjct: 215 DKSGNFPPLPTSTGISGG--AIAGISIGAIAVVLLLAGLVYVGYYRKKAQKVSLLSSEDR 272
Query: 1263 YHDHAMGNKFASGLNLEKNADSGHLNDGVFSELTDISMDKSLEFSYKELAEATDNFSLAK 1084
H + G + ++ + K ADSG L +G EL+ I++DKS+EF+Y+ELA AT++FS+A
Sbjct: 273 LHQSSHGPESST---IVKAADSGRLANGNSPELSGITVDKSVEFTYEELATATNDFSIAN 329
Query: 1083 KIGQGGFGAVYYAELRGEKAAVKKMDMHATKAFLAELKVLTHVHHLNLVRLIGYSVESSL 904
KIGQGGFGAVYYAELRGEKAA+KKMDM AT+ FLAELKVLTHVHHLNLVRLIGY VE SL
Sbjct: 330 KIGQGGFGAVYYAELRGEKAAIKKMDMEATREFLAELKVLTHVHHLNLVRLIGYCVEGSL 389
Query: 903 LIVYEYIDNGNLCQHLRGLSGKGPLSWSSRLQISLDSARGLEYIHEHTNPAYIHRDIKSP 724
+VYEY++NGN+ QHLRG +G+ PL WSSR+QI+LDSARGLEYIHEHT P YIHRDIK+
Sbjct: 390 FLVYEYVENGNIGQHLRG-TGRDPLPWSSRVQIALDSARGLEYIHEHTVPVYIHRDIKTA 448
Query: 723 NILLDKQLRAKVADFGLTKLVEVGTGSLQTRLVGTFGYMPPEYAQYGEVSTKIDVYAFGV 544
NIL+DK AKVADFGLTKL EVG+ SLQTRLVGTFGYMPPEYAQYG+VS K+DVYAFGV
Sbjct: 449 NILIDKNFHAKVADFGLTKLTEVGSSSLQTRLVGTFGYMPPEYAQYGDVSPKVDVYAFGV 508
Query: 543 LLYELISAREAIGNSKDVSTRTMGLVTLFEEVMNLPDPTTDLRKLVDPSF*DSPACESLY 364
+LYELISA+EAI + T + GLV LFEEV+N P+P DLRK+VDP + +S+
Sbjct: 509 VLYELISAKEAIVKPNESVTESKGLVGLFEEVLNQPEPDEDLRKVVDPRLGNDYPLDSVR 568
Query: 363 K----PKPCT 346
K K CT
Sbjct: 569 KMAQLAKACT 578
Score = 83.6 bits (205), Expect = 1e-13
Identities = 42/68 (61%), Positives = 55/68 (80%), Gaps = 1/68 (1%)
Frame = -3
Query: 428 PRISANW-LIPVFRIAQLAKACTNPNPVLRPSMRAVVVALMTISSGTENWYVGSLDENQS 252
PR+ ++ L V ++AQLAKACT+ NP++RPSMR++VVALMT+SS TE+W VGS NQ
Sbjct: 556 PRLGNDYPLDSVRKMAQLAKACTHENPLIRPSMRSIVVALMTLSSSTEDWDVGSFYGNQG 615
Query: 251 LVNLMSGR 228
L+NLMSGR
Sbjct: 616 LINLMSGR 623