BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00021585
         (2406 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI19437.3| unnamed protein product [Vitis vinifera]              430   e-118
ref|XP_002285122.1| PREDICTED: metal transporter Nramp2-like [Vi...   430   e-118
ref|XP_003523990.1| PREDICTED: metal transporter Nramp2-like [Gl...   425   e-116
ref|NP_175157.1| metal transporter Nramp2 [Arabidopsis thaliana]...   421   e-115
ref|XP_003636821.1| Natural resistance-associated macrophage pro...   420   e-114

>emb|CBI19437.3| unnamed protein product [Vitis vinifera]
          Length = 414

 Score =  430 bits (1106), Expect = e-118
 Identities = 205/289 (70%), Positives = 249/289 (86%)
 Frame = -3

Query: 2404 GLLVPKLGRKASQQAVGIVGCVITPHNIFLHSALVQSRKVDPTKRGCVQEALNYYTIESS 2225
            G+LVPKL  K  +QAVG+VGCVI PHN+FLHSALVQSRK+DP ++G VQEALNYY+IES+
Sbjct: 123  GVLVPKLSSKTIRQAVGVVGCVIMPHNVFLHSALVQSRKIDPHRKGRVQEALNYYSIEST 182

Query: 2224 IAVFVTFVINLCVTTVFAKGFYNTKEADSLGLLNAGDFLQERYGGGLFPILYIWGIGLLA 2045
            +A+ ++F+INL VTTVFAKGFY TK+AD +GL+NAG +L+E+YGGG+FPILY+WGIGLLA
Sbjct: 183  VALLISFMINLFVTTVFAKGFYGTKQADGIGLVNAGQYLEEKYGGGVFPILYVWGIGLLA 242

Query: 2044 AGQSSTLTGTYAGQFIMGGFLDFRLRKWLRSLITRSCAIVPTMIVAVVYDRSEASLDILN 1865
            AGQSST+TGTYAGQFIMGGFL+ RL+KWLR+LITRS AIVPTMIVA+V++RSEASLD+LN
Sbjct: 243  AGQSSTITGTYAGQFIMGGFLNLRLKKWLRALITRSFAIVPTMIVALVFNRSEASLDVLN 302

Query: 1864 EWLNVLQGIQIPFALIPLLVLVADEHLMGVFKIGPVFECIMWSVAALVTVINGYVLLDFF 1685
            EWLNVLQ +QIPFALIPLL LV+ E +MGVFK+GP+ E + W+VAALV VINGY+LLDFF
Sbjct: 303  EWLNVLQSMQIPFALIPLLTLVSKEQVMGVFKVGPLLERVAWTVAALVIVINGYLLLDFF 362

Query: 1684 SSEVKGLLLGILVCIMITAYAAFILYLISHGNALPSTWFNGLRSKRLGY 1538
             +EV G+L G LVC     Y AFI YL+SHG  L STWF+ + SKR  +
Sbjct: 363  MAEVDGVLFGFLVCAGTALYVAFIFYLVSHGGVLSSTWFSLVTSKRFPF 411


>ref|XP_002285122.1| PREDICTED: metal transporter Nramp2-like [Vitis vinifera]
          Length = 536

 Score =  430 bits (1106), Expect = e-118
 Identities = 205/289 (70%), Positives = 249/289 (86%)
 Frame = -3

Query: 2404 GLLVPKLGRKASQQAVGIVGCVITPHNIFLHSALVQSRKVDPTKRGCVQEALNYYTIESS 2225
            G+LVPKL  K  +QAVG+VGCVI PHN+FLHSALVQSRK+DP ++G VQEALNYY+IES+
Sbjct: 245  GVLVPKLSSKTIRQAVGVVGCVIMPHNVFLHSALVQSRKIDPHRKGRVQEALNYYSIEST 304

Query: 2224 IAVFVTFVINLCVTTVFAKGFYNTKEADSLGLLNAGDFLQERYGGGLFPILYIWGIGLLA 2045
            +A+ ++F+INL VTTVFAKGFY TK+AD +GL+NAG +L+E+YGGG+FPILY+WGIGLLA
Sbjct: 305  VALLISFMINLFVTTVFAKGFYGTKQADGIGLVNAGQYLEEKYGGGVFPILYVWGIGLLA 364

Query: 2044 AGQSSTLTGTYAGQFIMGGFLDFRLRKWLRSLITRSCAIVPTMIVAVVYDRSEASLDILN 1865
            AGQSST+TGTYAGQFIMGGFL+ RL+KWLR+LITRS AIVPTMIVA+V++RSEASLD+LN
Sbjct: 365  AGQSSTITGTYAGQFIMGGFLNLRLKKWLRALITRSFAIVPTMIVALVFNRSEASLDVLN 424

Query: 1864 EWLNVLQGIQIPFALIPLLVLVADEHLMGVFKIGPVFECIMWSVAALVTVINGYVLLDFF 1685
            EWLNVLQ +QIPFALIPLL LV+ E +MGVFK+GP+ E + W+VAALV VINGY+LLDFF
Sbjct: 425  EWLNVLQSMQIPFALIPLLTLVSKEQVMGVFKVGPLLERVAWTVAALVIVINGYLLLDFF 484

Query: 1684 SSEVKGLLLGILVCIMITAYAAFILYLISHGNALPSTWFNGLRSKRLGY 1538
             +EV G+L G LVC     Y AFI YL+SHG  L STWF+ + SKR  +
Sbjct: 485  MAEVDGVLFGFLVCAGTALYVAFIFYLVSHGGVLSSTWFSLVTSKRFPF 533


>ref|XP_003523990.1| PREDICTED: metal transporter Nramp2-like [Glycine max]
          Length = 518

 Score =  425 bits (1092), Expect = e-116
 Identities = 204/282 (72%), Positives = 243/282 (86%)
 Frame = -3

Query: 2404 GLLVPKLGRKASQQAVGIVGCVITPHNIFLHSALVQSRKVDPTKRGCVQEALNYYTIESS 2225
            G+LVP+LG K  +QAVG+VGCVI PHN+FLHSALVQSRKVDP K G VQEALNYY+IESS
Sbjct: 228  GILVPRLGSKTIRQAVGVVGCVIMPHNVFLHSALVQSRKVDPKKIGRVQEALNYYSIESS 287

Query: 2224 IAVFVTFVINLCVTTVFAKGFYNTKEADSLGLLNAGDFLQERYGGGLFPILYIWGIGLLA 2045
             A+ V+F+INL VTTVFAKGFY TK+ADS+GL+NAG +L+E+YGGG+FPILYIWGIGLLA
Sbjct: 288  AALAVSFMINLFVTTVFAKGFYGTKQADSIGLVNAGQYLEEKYGGGVFPILYIWGIGLLA 347

Query: 2044 AGQSSTLTGTYAGQFIMGGFLDFRLRKWLRSLITRSCAIVPTMIVAVVYDRSEASLDILN 1865
            AGQSST+TGTYAGQFIMGGFL+ RL+KWLR+LITRS AIVPT+IVA+V++RSE SLD+LN
Sbjct: 348  AGQSSTITGTYAGQFIMGGFLNLRLKKWLRALITRSFAIVPTIIVAIVFNRSEGSLDVLN 407

Query: 1864 EWLNVLQGIQIPFALIPLLVLVADEHLMGVFKIGPVFECIMWSVAALVTVINGYVLLDFF 1685
            EWLNVLQ +QIPFALIPLL LV+ E +MG F++GPV E + W+VA L+ +INGY+LLDFF
Sbjct: 408  EWLNVLQSMQIPFALIPLLTLVSKEQIMGTFRVGPVLERVAWTVAGLIIIINGYLLLDFF 467

Query: 1684 SSEVKGLLLGILVCIMITAYAAFILYLISHGNALPSTWFNGL 1559
             SEV G+LLG+L C   TAY AFI+YL+S    LPS W N L
Sbjct: 468  VSEVNGILLGLLACSCTTAYIAFIVYLVSQSGILPSAWVNRL 509


>ref|NP_175157.1| metal transporter Nramp2 [Arabidopsis thaliana]
            gi|29839611|sp|Q9C6B2.1|NRAM2_ARATH RecName: Full=Metal
            transporter Nramp2; Short=AtNramp2
            gi|12325401|gb|AAG52643.1|AC079677_7 stress response
            protein Nramp2; 19015-21280 [Arabidopsis thaliana]
            gi|15294258|gb|AAK95306.1|AF410320_1 At1g47240/F8G22_4
            [Arabidopsis thaliana] gi|20857010|gb|AAM26695.1|
            At1g47240/F8G22_4 [Arabidopsis thaliana]
            gi|332194021|gb|AEE32142.1| metal transporter Nramp2
            [Arabidopsis thaliana]
          Length = 530

 Score =  421 bits (1081), Expect = e-115
 Identities = 197/278 (70%), Positives = 242/278 (87%)
 Frame = -3

Query: 2404 GLLVPKLGRKASQQAVGIVGCVITPHNIFLHSALVQSRKVDPTKRGCVQEALNYYTIESS 2225
            G+L+P+L  K  +QAVG+VGCVI PHN+FLHSALVQSRK+DP ++  VQEALNYY IESS
Sbjct: 237  GILLPRLSSKTIRQAVGVVGCVIMPHNVFLHSALVQSRKIDPKRKSRVQEALNYYLIESS 296

Query: 2224 IAVFVTFVINLCVTTVFAKGFYNTKEADSLGLLNAGDFLQERYGGGLFPILYIWGIGLLA 2045
            +A+F++F+INL VTTVFAKGFY T++A+++GL+NAG +LQE++GGGL PILYIWGIGLLA
Sbjct: 297  VALFISFMINLFVTTVFAKGFYGTEKANNIGLVNAGQYLQEKFGGGLLPILYIWGIGLLA 356

Query: 2044 AGQSSTLTGTYAGQFIMGGFLDFRLRKWLRSLITRSCAIVPTMIVAVVYDRSEASLDILN 1865
            AGQSST+TGTYAGQFIMGGFL+ RL+KW+R++ITRSCAIVPTMIVA+V++ SEASLD+LN
Sbjct: 357  AGQSSTITGTYAGQFIMGGFLNLRLKKWMRAVITRSCAIVPTMIVAIVFNTSEASLDVLN 416

Query: 1864 EWLNVLQGIQIPFALIPLLVLVADEHLMGVFKIGPVFECIMWSVAALVTVINGYVLLDFF 1685
            EWLNVLQ +QIPFAL+PLL LV+ E +MG FKIGP+ + I W+VAALV +INGY+LLDFF
Sbjct: 417  EWLNVLQSVQIPFALLPLLTLVSKEEIMGDFKIGPILQRIAWTVAALVMIINGYLLLDFF 476

Query: 1684 SSEVKGLLLGILVCIMITAYAAFILYLISHGNALPSTW 1571
             SEV G L G+ VC+  TAY AFI+YLISH N  PS W
Sbjct: 477  VSEVDGFLFGVTVCVWTTAYIAFIVYLISHSNFFPSPW 514


>ref|XP_003636821.1| Natural resistance-associated macrophage protein [Medicago
            truncatula] gi|358348881|ref|XP_003638470.1| Natural
            resistance-associated macrophage protein [Medicago
            truncatula] gi|355502756|gb|AES83959.1| Natural
            resistance-associated macrophage protein [Medicago
            truncatula] gi|355504405|gb|AES85608.1| Natural
            resistance-associated macrophage protein [Medicago
            truncatula]
          Length = 528

 Score =  420 bits (1079), Expect = e-114
 Identities = 201/276 (72%), Positives = 238/276 (86%)
 Frame = -3

Query: 2404 GLLVPKLGRKASQQAVGIVGCVITPHNIFLHSALVQSRKVDPTKRGCVQEALNYYTIESS 2225
            G+L+P+L  K   QAVG+VGCVI PHN+FLHSALVQSRK+DP K+G VQEALNYY IES+
Sbjct: 238  GILIPRLSSKTIHQAVGVVGCVIMPHNVFLHSALVQSRKIDPNKKGRVQEALNYYNIEST 297

Query: 2224 IAVFVTFVINLCVTTVFAKGFYNTKEADSLGLLNAGDFLQERYGGGLFPILYIWGIGLLA 2045
            +A+ VTF+INL VTTVFAKGFY TK+A+S+GL+NAG +L+E+YGGGLFPILYIWGIGLLA
Sbjct: 298  VALSVTFMINLFVTTVFAKGFYGTKQANSIGLVNAGQYLEEKYGGGLFPILYIWGIGLLA 357

Query: 2044 AGQSSTLTGTYAGQFIMGGFLDFRLRKWLRSLITRSCAIVPTMIVAVVYDRSEASLDILN 1865
            AGQSST+TGTYAGQFIMGGFL+ RL+KWLR+LITRSCAIVPT+IVA+VY+RSEASLD+LN
Sbjct: 358  AGQSSTITGTYAGQFIMGGFLNLRLKKWLRALITRSCAIVPTIIVAIVYNRSEASLDVLN 417

Query: 1864 EWLNVLQGIQIPFALIPLLVLVADEHLMGVFKIGPVFECIMWSVAALVTVINGYVLLDFF 1685
            EWLNVLQ IQIPFALIPLL LV+ E +MG FK+GPV E + W+VAAL+ VINGY+L+DFF
Sbjct: 418  EWLNVLQSIQIPFALIPLLTLVSKERIMGSFKVGPVLERVAWTVAALIIVINGYLLVDFF 477

Query: 1684 SSEVKGLLLGILVCIMITAYAAFILYLISHGNALPS 1577
             SEV  +L G + C     Y AFI+YLIS   ALPS
Sbjct: 478  LSEVNSVLFGFVACSCTVTYIAFIVYLISQSGALPS 513