BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00021548
         (1019 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAI48083.1| MIS12 homologue [Nicotiana tabacum] gi|262263171...   236   5e-60
ref|XP_002270531.1| PREDICTED: uncharacterized protein LOC100267...   236   5e-60
dbj|BAI48082.1| MIS12 homologue [Nicotiana tabacum]                   232   1e-58
dbj|BAI48087.1| kinetochore protein [Nicotiana sylvestris]            230   4e-58
ref|XP_002328650.1| predicted protein [Populus trichocarpa] gi|2...   215   2e-53

>dbj|BAI48083.1| MIS12 homologue [Nicotiana tabacum] gi|262263171|dbj|BAI48088.1|
           kinetochore protein [Nicotiana tomentosiformis]
          Length = 246

 Score =  236 bits (603), Expect = 5e-60
 Identities = 126/241 (52%), Positives = 165/241 (68%)
 Frame = -3

Query: 960 MEGSESEAIFDSLNLNPQFFXXXXXXXXXXXXXDAFLFFHQEASSVLKIEGTERAEELKK 781
           MEGSESE +FDSLNLNP+ F             DAF FFHQEA+++LK EGT R+E+LK+
Sbjct: 1   MEGSESEKVFDSLNLNPKLFVNEALNIVDELVDDAFDFFHQEAANLLKTEGTNRSEDLKE 60

Query: 780 GVDSLKNMVQSALDGPLTMWEKYCLRHCFVVPQGFSLPTTNESSGEIAMDIDGNDDAELD 601
           GV  +KNMVQ  LD  L++WEKYCL +CF VPQGFSLP  +  SG+ + DI+  ++ ELD
Sbjct: 61  GVAQIKNMVQLTLDKRLSLWEKYCLHNCFKVPQGFSLPKADGPSGDTSFDINAVENPELD 120

Query: 600 MQLKSLREKLVLVGKESAELNKELKMLERQSSLSDQHSRSLDEAVQLCEQYIDHDIFEEL 421
            +L  LR K+  VGKESAELN+EL+ LE QS LS   + SL EA++L +Q   ++ FEEL
Sbjct: 121 EKLDFLRNKISEVGKESAELNRELQALEGQSMLSGHSAASLTEALELYQQLAVNEKFEEL 180

Query: 420 KRTASELHTMVEKLKTKRVEETDCIKMDKMHMSNGSVFGMNRGNGLTNTTLEELQQFIND 241
            RTAS+  + VE L T+ VE+T+  +  K+  SNG VF  N   GL + TLEELQ F++D
Sbjct: 181 VRTASDFQSKVENLATRMVEDTEHRRAKKIRTSNGEVFRSNNDEGLLSATLEELQVFVDD 240

Query: 240 M 238
           +
Sbjct: 241 I 241


>ref|XP_002270531.1| PREDICTED: uncharacterized protein LOC100267948 [Vitis vinifera]
           gi|297734134|emb|CBI15381.3| unnamed protein product
           [Vitis vinifera]
          Length = 244

 Score =  236 bits (603), Expect = 5e-60
 Identities = 120/238 (50%), Positives = 168/238 (70%)
 Frame = -3

Query: 960 MEGSESEAIFDSLNLNPQFFXXXXXXXXXXXXXDAFLFFHQEASSVLKIEGTERAEELKK 781
           MEGSESEAIF+SLNLNPQ F              AF F+ QEAS++L+ +GT+R+ ++ K
Sbjct: 1   MEGSESEAIFESLNLNPQLFINEVLNLVDDLLDGAFDFYQQEASNLLRTDGTDRSGDINK 60

Query: 780 GVDSLKNMVQSALDGPLTMWEKYCLRHCFVVPQGFSLPTTNESSGEIAMDIDGNDDAELD 601
           G+  ++NM+Q  LD  L MWEKYCLRHCF VP+GF +P  +E+ G+ +++ D     ELD
Sbjct: 61  GIGYIRNMIQGVLDKRLEMWEKYCLRHCFAVPEGFLMPKASETPGDSSINKDVLSGLELD 120

Query: 600 MQLKSLREKLVLVGKESAELNKELKMLERQSSLSDQHSRSLDEAVQLCEQYIDHDIFEEL 421
            QL SLR+KL +V KESAELN+EL+ LERQS LS+Q + S++EA+QL EQ   HD+F+E+
Sbjct: 121 AQLDSLRDKLTVVAKESAELNRELQALERQSVLSNQCTGSVNEALQLYEQNKVHDMFQEM 180

Query: 420 KRTASELHTMVEKLKTKRVEETDCIKMDKMHMSNGSVFGMNRGNGLTNTTLEELQQFI 247
            RTASEL T +EK+KTKR+E  + +K ++++  +  +   NR  GL N  LE+LQ+F+
Sbjct: 181 MRTASELRTKMEKMKTKRMEGIERVKTERIYNPDRDLSMRNRDKGLFNAELEDLQEFL 238


>dbj|BAI48082.1| MIS12 homologue [Nicotiana tabacum]
          Length = 246

 Score =  232 bits (591), Expect = 1e-58
 Identities = 123/241 (51%), Positives = 164/241 (68%)
 Frame = -3

Query: 960 MEGSESEAIFDSLNLNPQFFXXXXXXXXXXXXXDAFLFFHQEASSVLKIEGTERAEELKK 781
           MEGSESE +FDSLNLNP+ F             DAF FFH+EA+++LK EGT R+E+LK+
Sbjct: 1   MEGSESEKVFDSLNLNPKHFVNEALNIADELVDDAFDFFHREAANLLKTEGTNRSEDLKE 60

Query: 780 GVDSLKNMVQSALDGPLTMWEKYCLRHCFVVPQGFSLPTTNESSGEIAMDIDGNDDAELD 601
           GV  +KNMVQ  LD  L+ WEKYCL  CF VPQGFSLP  +  SG+ + DI+  ++ ELD
Sbjct: 61  GVAQIKNMVQLTLDKRLSYWEKYCLDKCFKVPQGFSLPKADGPSGDTSFDINAVENPELD 120

Query: 600 MQLKSLREKLVLVGKESAELNKELKMLERQSSLSDQHSRSLDEAVQLCEQYIDHDIFEEL 421
            +L  LR K+  VGKESAEL++EL+ LERQS LS   + SL EA++L +Q    + FEEL
Sbjct: 121 EKLDFLRNKISEVGKESAELHRELRALERQSMLSGHSAASLTEALELYQQLAVDEKFEEL 180

Query: 420 KRTASELHTMVEKLKTKRVEETDCIKMDKMHMSNGSVFGMNRGNGLTNTTLEELQQFIND 241
            RTAS+  T VE L T+ VE ++  +  ++  SNG +F ++   GL + TLEELQ+F+ND
Sbjct: 181 VRTASDFQTKVENLATRMVENSEHRRAKRIRTSNGEMFRLSNDGGLLSATLEELQEFVND 240

Query: 240 M 238
           +
Sbjct: 241 I 241


>dbj|BAI48087.1| kinetochore protein [Nicotiana sylvestris]
          Length = 246

 Score =  230 bits (587), Expect = 4e-58
 Identities = 123/241 (51%), Positives = 163/241 (67%)
 Frame = -3

Query: 960 MEGSESEAIFDSLNLNPQFFXXXXXXXXXXXXXDAFLFFHQEASSVLKIEGTERAEELKK 781
           MEGSESE +FDSLNLNP+ F             DAF FFH+EA+++LK EGT R+E+LK+
Sbjct: 1   MEGSESEKVFDSLNLNPKHFVNEALNIADELVDDAFDFFHREAANLLKTEGTNRSEDLKE 60

Query: 780 GVDSLKNMVQSALDGPLTMWEKYCLRHCFVVPQGFSLPTTNESSGEIAMDIDGNDDAELD 601
           GV  +KNMVQ  LD  L+ WEKYCL  CF VPQGFSLP  +  SG+ + DI+  ++ ELD
Sbjct: 61  GVAQIKNMVQLTLDKRLSYWEKYCLDKCFKVPQGFSLPKADGPSGDTSFDINAVENPELD 120

Query: 600 MQLKSLREKLVLVGKESAELNKELKMLERQSSLSDQHSRSLDEAVQLCEQYIDHDIFEEL 421
            +L  LR K+  VGKESAEL++EL+ LERQS LS   + SL EA++L +Q    + FEEL
Sbjct: 121 EKLDFLRNKISEVGKESAELHRELRALERQSMLSGHSAASLTEALELYQQLAVDEKFEEL 180

Query: 420 KRTASELHTMVEKLKTKRVEETDCIKMDKMHMSNGSVFGMNRGNGLTNTTLEELQQFIND 241
            RTAS   T VE L T+ VE ++  +  ++  SNG +F ++   GL + TLEELQ+F+ND
Sbjct: 181 VRTASGFQTKVENLATRMVENSEHRRAKRIRTSNGEMFRLSNDGGLLSATLEELQEFVND 240

Query: 240 M 238
           +
Sbjct: 241 I 241


>ref|XP_002328650.1| predicted protein [Populus trichocarpa] gi|222838826|gb|EEE77177.1|
           predicted protein [Populus trichocarpa]
          Length = 243

 Score =  215 bits (547), Expect = 2e-53
 Identities = 114/244 (46%), Positives = 160/244 (65%)
 Frame = -3

Query: 960 MEGSESEAIFDSLNLNPQFFXXXXXXXXXXXXXDAFLFFHQEASSVLKIEGTERAEELKK 781
           MEGSESEA+F+SLNLNPQ F             DAF FFHQEAS++LK +GT R++ + +
Sbjct: 1   MEGSESEAVFESLNLNPQLFINETLNTVDDLLDDAFHFFHQEASTLLKTDGTSRSQFISE 60

Query: 780 GVDSLKNMVQSALDGPLTMWEKYCLRHCFVVPQGFSLPTTNESSGEIAMDIDGNDDAELD 601
           G+  +++M+QS LD  L MWEKYCL H F VP GFSLP T ES  E     D   D +LD
Sbjct: 61  GIAYVRDMIQSDLDTRLGMWEKYCLHHVFAVPAGFSLPQT-ESPAETFTCQDLLSDPDLD 119

Query: 600 MQLKSLREKLVLVGKESAELNKELKMLERQSSLSDQHSRSLDEAVQLCEQYIDHDIFEEL 421
            QL  LR +L +VGKES+ELN EL+ LERQS+ SD  +  + EA+ L +Q    D+++++
Sbjct: 120 AQLDILRNRLTMVGKESSELNHELQALERQSASSDHCAELVSEALLLYDQASTQDMYQDM 179

Query: 420 KRTASELHTMVEKLKTKRVEETDCIKMDKMHMSNGSVFGMNRGNGLTNTTLEELQQFIND 241
             T SELH  +EKL+T++VE  +CI+  +++  N  +   N   GL N T+E++Q+F+ D
Sbjct: 180 LSTMSELHEKMEKLRTRKVENLECIRAKRINDPNRDLLASNYRKGLPNATVEDIQEFMAD 239

Query: 240 M*YM 229
           + +M
Sbjct: 240 LKHM 243