BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00021540
         (1613 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279425.2| PREDICTED: putative methyltransferase NSUN5-...   606   e-171
emb|CBI20685.3| unnamed protein product [Vitis vinifera]              606   e-171
dbj|BAK61830.1| NOL1/NOP2/sun family protein [Citrus unshiu]          590   e-166
ref|XP_004141333.1| PREDICTED: putative methyltransferase NSUN5-...   579   e-163
ref|XP_002309481.1| predicted protein [Populus trichocarpa] gi|2...   568   e-159

>ref|XP_002279425.2| PREDICTED: putative methyltransferase NSUN5-like [Vitis vinifera]
          Length = 508

 Score =  606 bits (1563), Expect = e-171
 Identities = 324/497 (65%), Positives = 382/497 (76%), Gaps = 7/497 (1%)
 Frame = -3

Query: 1599 STANMAPATKQTKSGRLSNAEKSSFFARREAAKVLRSVLDGDARRQAVASIKTLVYSPSV 1420
            + A  A   K+ K  RLS A++SS+FARREAAKVL+ VL GDA+R+AV SIK+LVYSPSV
Sbjct: 6    TAATTASRAKKPKEQRLSKADRSSYFARREAAKVLQCVLQGDAKRRAVGSIKSLVYSPSV 65

Query: 1419 RNKKATFALVCQTLKYLPYIKDVLESSGVLNSKWKKQGPLMYLITYDILFGKNVSLVGDA 1240
            RNKKATFALVCQTLK+L  IKDVL+S+ +LN+KWK+Q  LMY+ITYDILFG+ +S VGDA
Sbjct: 66   RNKKATFALVCQTLKHLSIIKDVLDSARILNAKWKRQEGLMYIITYDILFGQEISSVGDA 125

Query: 1239 EKFLMHKKDALLSALAKILVKNRVKNVQDLMAPRS--DRSKPRYVRVNTLKLNVETALDE 1066
            EKFL+ +KDA+  ALA++LV+ +VK V+DLMA     D SKPR+VRVNTLKL+VE+A  E
Sbjct: 126  EKFLLLQKDAIQLALARLLVRKKVKRVEDLMALYQIPDVSKPRFVRVNTLKLDVESAFHE 185

Query: 1065 LRKRYKVHKXXXXXXXXXXXPCTDLHEHPLVKNGSVFLQGKASSLVAVALDPSPGWEVLD 886
            L K+  V K           P +DLH HPLV+NGSVF+QGKASS+VAVAL P PGWEVLD
Sbjct: 186  LGKQNMVQKDDMVPDLLILPPGSDLHNHPLVRNGSVFMQGKASSMVAVALGPEPGWEVLD 245

Query: 885  ACSAPGNKTVHLAALMRGKGKIIACELNKERVERLKHTIKQAGATNVEVLHKDFLKLSPV 706
            ACSAPGNKTVHLAALM GKGKIIACEL+ ERV+RL+ T++ +GA N+EVLH DFL L+P+
Sbjct: 246  ACSAPGNKTVHLAALMNGKGKIIACELDNERVKRLEDTVRLSGAANIEVLHGDFLNLNPM 305

Query: 705  DPSYSKVRAILLDPSCSGSGTAVERLDHLLPSYQQGHA-DPSDMHRLTKLSAFQKMALEH 529
            DPSYSKVRAILLDPSCSGSGT  ERLDHLLPSY  G A D +   RL KL+AFQK ALEH
Sbjct: 306  DPSYSKVRAILLDPSCSGSGTTAERLDHLLPSYAAGDATDVAGTERLNKLAAFQKKALEH 365

Query: 528  ALSFPGVERIVYSTCSIHQIENEDVIKSVLPLATSCGFQLSTPFPQWPRRGLPVLEEFKH 349
            ALSFP VE++VYSTCSIHQIENEDVIKSVLPLA S GFQL+TPFPQWPRRGLPV E  +H
Sbjct: 366  ALSFPAVEKVVYSTCSIHQIENEDVIKSVLPLAASHGFQLATPFPQWPRRGLPVFEGSEH 425

Query: 348  LLRTDPVEDKEGFFIALFVRSIHXXXXXXXXXXXXNAVNPRKIVKVRKGKN----MKSRF 181
            LLRT PVEDKEGFFIALFVR                 +  RK  KV+  K       ++ 
Sbjct: 426  LLRTHPVEDKEGFFIALFVRK-------SIARSLEQPIKTRKNNKVKSAKRNATMNHNKS 478

Query: 180  VASCFFPRMSRILLNPY 130
            V    F R+S++LL+ +
Sbjct: 479  VMPFPFTRLSKLLLHSH 495


>emb|CBI20685.3| unnamed protein product [Vitis vinifera]
          Length = 626

 Score =  606 bits (1563), Expect = e-171
 Identities = 324/497 (65%), Positives = 382/497 (76%), Gaps = 7/497 (1%)
 Frame = -3

Query: 1599 STANMAPATKQTKSGRLSNAEKSSFFARREAAKVLRSVLDGDARRQAVASIKTLVYSPSV 1420
            + A  A   K+ K  RLS A++SS+FARREAAKVL+ VL GDA+R+AV SIK+LVYSPSV
Sbjct: 6    TAATTASRAKKPKEQRLSKADRSSYFARREAAKVLQCVLQGDAKRRAVGSIKSLVYSPSV 65

Query: 1419 RNKKATFALVCQTLKYLPYIKDVLESSGVLNSKWKKQGPLMYLITYDILFGKNVSLVGDA 1240
            RNKKATFALVCQTLK+L  IKDVL+S+ +LN+KWK+Q  LMY+ITYDILFG+ +S VGDA
Sbjct: 66   RNKKATFALVCQTLKHLSIIKDVLDSARILNAKWKRQEGLMYIITYDILFGQEISSVGDA 125

Query: 1239 EKFLMHKKDALLSALAKILVKNRVKNVQDLMAPRS--DRSKPRYVRVNTLKLNVETALDE 1066
            EKFL+ +KDA+  ALA++LV+ +VK V+DLMA     D SKPR+VRVNTLKL+VE+A  E
Sbjct: 126  EKFLLLQKDAIQLALARLLVRKKVKRVEDLMALYQIPDVSKPRFVRVNTLKLDVESAFHE 185

Query: 1065 LRKRYKVHKXXXXXXXXXXXPCTDLHEHPLVKNGSVFLQGKASSLVAVALDPSPGWEVLD 886
            L K+  V K           P +DLH HPLV+NGSVF+QGKASS+VAVAL P PGWEVLD
Sbjct: 186  LGKQNMVQKDDMVPDLLILPPGSDLHNHPLVRNGSVFMQGKASSMVAVALGPEPGWEVLD 245

Query: 885  ACSAPGNKTVHLAALMRGKGKIIACELNKERVERLKHTIKQAGATNVEVLHKDFLKLSPV 706
            ACSAPGNKTVHLAALM GKGKIIACEL+ ERV+RL+ T++ +GA N+EVLH DFL L+P+
Sbjct: 246  ACSAPGNKTVHLAALMNGKGKIIACELDNERVKRLEDTVRLSGAANIEVLHGDFLNLNPM 305

Query: 705  DPSYSKVRAILLDPSCSGSGTAVERLDHLLPSYQQGHA-DPSDMHRLTKLSAFQKMALEH 529
            DPSYSKVRAILLDPSCSGSGT  ERLDHLLPSY  G A D +   RL KL+AFQK ALEH
Sbjct: 306  DPSYSKVRAILLDPSCSGSGTTAERLDHLLPSYAAGDATDVAGTERLNKLAAFQKKALEH 365

Query: 528  ALSFPGVERIVYSTCSIHQIENEDVIKSVLPLATSCGFQLSTPFPQWPRRGLPVLEEFKH 349
            ALSFP VE++VYSTCSIHQIENEDVIKSVLPLA S GFQL+TPFPQWPRRGLPV E  +H
Sbjct: 366  ALSFPAVEKVVYSTCSIHQIENEDVIKSVLPLAASHGFQLATPFPQWPRRGLPVFEGSEH 425

Query: 348  LLRTDPVEDKEGFFIALFVRSIHXXXXXXXXXXXXNAVNPRKIVKVRKGKN----MKSRF 181
            LLRT PVEDKEGFFIALFVR                 +  RK  KV+  K       ++ 
Sbjct: 426  LLRTHPVEDKEGFFIALFVRK-------SIARSLEQPIKTRKNNKVKSAKRNATMNHNKS 478

Query: 180  VASCFFPRMSRILLNPY 130
            V    F R+S++LL+ +
Sbjct: 479  VMPFPFTRLSKLLLHSH 495


>dbj|BAK61830.1| NOL1/NOP2/sun family protein [Citrus unshiu]
          Length = 534

 Score =  590 bits (1520), Expect = e-166
 Identities = 310/475 (65%), Positives = 366/475 (77%), Gaps = 38/475 (8%)
 Frame = -3

Query: 1599 STANMAPATKQTKSGRLSNAEKSSFFARREAAKVLRSVLDGDARRQAVASIKTLVYSPSV 1420
            +TA    + K +++GRLSNAE+S++FARREAAKVLR VL GDARR+AV SIK+LVYSPSV
Sbjct: 8    ATAPSGASAKTSRNGRLSNAERSAYFARREAAKVLRLVLRGDARRRAVGSIKSLVYSPSV 67

Query: 1419 RNKKATFALVCQTLKYLPYIKDVLESSGVLNSKWKKQGPLMYLITYDILFGKNVSLVGDA 1240
            +NKKAT+ALVCQTLK+L  IK VL+S+ +LNSKWK+Q  L+Y++TYDILFG+ +SLVGDA
Sbjct: 68   KNKKATYALVCQTLKHLSIIKQVLDSASILNSKWKRQEELVYILTYDILFGQEISLVGDA 127

Query: 1239 EKFLMHKKDALLSALAKILVKNRVKNVQDLMA--PRSDRSKPRYVRVNTLKLNVETALDE 1066
            EKFLM  K A+  ALA++LV+N+VK+++DLMA     D  KPRYVRVNTLK++V++A+ E
Sbjct: 128  EKFLMLHKGAIQLALAQLLVRNKVKSIEDLMALYQTPDVPKPRYVRVNTLKMDVDSAVLE 187

Query: 1065 LRKRYKVHKXXXXXXXXXXXPCTDLHEHPLVKNGSVFLQGKASSLVAVALDPSPGWEVLD 886
            L K++ V K           P  DLH HPL+ NG VFLQGKASS+VA AL P PGW+VLD
Sbjct: 188  LGKQFVVQKDDLVPDLLILPPGCDLHVHPLIVNGCVFLQGKASSMVAAALAPKPGWKVLD 247

Query: 885  ACSAPGNKTVHLAALMRGKGKIIACELNKERVERLKHTIKQAGAT--------------- 751
            ACSAPGNKTVHLAALM+GKGKI+ACELNKERV RLK TIK +GA                
Sbjct: 248  ACSAPGNKTVHLAALMKGKGKIVACELNKERVRRLKDTIKLSGAAKFKVLLFVMMHYLYS 307

Query: 750  --------------------NVEVLHKDFLKLSPVDPSYSKVRAILLDPSCSGSGTAVER 631
                                ++EVLH DFL L P DP+YS+VRAILLDPSCSGSGTA ER
Sbjct: 308  MIHAFQLLSILFRNSLNQPEDIEVLHGDFLNLDPKDPAYSEVRAILLDPSCSGSGTAAER 367

Query: 630  LDHLLPSYQQGH-ADPSDMHRLTKLSAFQKMALEHALSFPGVERIVYSTCSIHQIENEDV 454
            LDHLLPS+  GH ADP++M RL KLSAFQK AL HALSFPGVER+VYSTCSIHQ+ENEDV
Sbjct: 368  LDHLLPSHASGHTADPTEMERLNKLSAFQKKALRHALSFPGVERVVYSTCSIHQVENEDV 427

Query: 453  IKSVLPLATSCGFQLSTPFPQWPRRGLPVLEEFKHLLRTDPVEDKEGFFIALFVR 289
            IKSVLP+A S GFQL+TPFPQW  RGLPV E  +HLLRTDPVEDKEGFFIALFV+
Sbjct: 428  IKSVLPIAMSFGFQLATPFPQWNCRGLPVFEGSEHLLRTDPVEDKEGFFIALFVK 482


>ref|XP_004141333.1| PREDICTED: putative methyltransferase NSUN5-like [Cucumis sativus]
            gi|449529369|ref|XP_004171672.1| PREDICTED: putative
            methyltransferase NSUN5-like [Cucumis sativus]
          Length = 509

 Score =  579 bits (1493), Expect = e-163
 Identities = 289/436 (66%), Positives = 355/436 (81%), Gaps = 3/436 (0%)
 Frame = -3

Query: 1593 ANMAPATKQTKSGRLSNAEKSSFFARREAAKVLRSVLDGDARRQAVASIKTLVYSPSVRN 1414
            A+ + A  +++  R+SNA++S++FARREAAKVLR VL+GDA+R+A+ASIKTLVY+PSVRN
Sbjct: 10   ASASGAAGKSEMRRMSNADRSAYFARREAAKVLRRVLEGDAQRRAIASIKTLVYAPSVRN 69

Query: 1413 KKATFALVCQTLKYLPYIKDVLESSGVLNSKWKKQGPLMYLITYDILFGKNVSLVGDAEK 1234
            KK TFALVC+TLKYLP IKDV+E++ +L++KWK+Q  L+Y+I YDIL G+   L GDAEK
Sbjct: 70   KKGTFALVCKTLKYLPVIKDVVEAADLLSNKWKRQKELIYVIMYDILLGQKTHLAGDAEK 129

Query: 1233 FLMHKKDALLSALAKILVKNRVKNVQDLMAPRSDR---SKPRYVRVNTLKLNVETALDEL 1063
            FLM ++ AL SA+ ++L K +  N++DL+A   D    S+PR+VRVNTLK++V TA++EL
Sbjct: 130  FLMRQQSALQSAVTQLLSKKKANNIEDLIARGDDGPGVSRPRFVRVNTLKMDVHTAIEEL 189

Query: 1062 RKRYKVHKXXXXXXXXXXXPCTDLHEHPLVKNGSVFLQGKASSLVAVALDPSPGWEVLDA 883
             KRY V K           P +DLH+HPLV +GS+FLQGKASS+VAVALDP PGWEVLDA
Sbjct: 190  GKRYAVQKDDMVSDLLILPPGSDLHDHPLVADGSIFLQGKASSMVAVALDPKPGWEVLDA 249

Query: 882  CSAPGNKTVHLAALMRGKGKIIACELNKERVERLKHTIKQAGATNVEVLHKDFLKLSPVD 703
            CSAPGNKTVHLA+LM GKG++IACELN+ RV+RL+HTIK +GA+N++VLH DFL L+P D
Sbjct: 250  CSAPGNKTVHLASLMHGKGRVIACELNENRVKRLRHTIKLSGASNIDVLHGDFLNLNPKD 309

Query: 702  PSYSKVRAILLDPSCSGSGTAVERLDHLLPSYQQGHADPSDMHRLTKLSAFQKMALEHAL 523
            PS SKVRAILLDPSCSGSGTA  RLDHLLPS+ +G     D+ RL KL+AFQ+ AL HA 
Sbjct: 310  PSLSKVRAILLDPSCSGSGTAAVRLDHLLPSHAEGTISGDDLERLNKLAAFQRKALAHAF 369

Query: 522  SFPGVERIVYSTCSIHQIENEDVIKSVLPLATSCGFQLSTPFPQWPRRGLPVLEEFKHLL 343
            SFP VE+IVYSTCSIHQIENEDV++SVLPLA S GFQL TPFP+W RRGLPV     HLL
Sbjct: 370  SFPAVEKIVYSTCSIHQIENEDVVQSVLPLAESRGFQLDTPFPRWQRRGLPVFAGANHLL 429

Query: 342  RTDPVEDKEGFFIALF 295
            RTDPVEDKEGFFIALF
Sbjct: 430  RTDPVEDKEGFFIALF 445


>ref|XP_002309481.1| predicted protein [Populus trichocarpa] gi|222855457|gb|EEE93004.1|
            predicted protein [Populus trichocarpa]
          Length = 433

 Score =  568 bits (1465), Expect = e-159
 Identities = 296/432 (68%), Positives = 349/432 (80%), Gaps = 3/432 (0%)
 Frame = -3

Query: 1575 TKQTKSGRLSNAEKSSFFARREAAKVLRSVLDGDARRQAVASIKTLVYSPSVRNKKATFA 1396
            T ++   RL+NAE+S++FARREAAKVL +VL GDA+RQAVASIK+LVYSPS+RNKKATFA
Sbjct: 6    TDKSAKPRLNNAERSAYFARREAAKVLHTVLQGDAKRQAVASIKSLVYSPSIRNKKATFA 65

Query: 1395 LVCQTLKYLPYIKDVLESSGVLNSKWKKQGPLMYLITYDILFGKNVSLVG-DAEKFLMHK 1219
            LVCQTLK+L  IKDVLE + +LNSKWK+Q  L+Y+I YD+LFGK +SLVG DAEKFL  +
Sbjct: 66   LVCQTLKHLRIIKDVLEIANILNSKWKRQEELIYIIAYDVLFGKGISLVGGDAEKFLACR 125

Query: 1218 KDALLSALAKILVKNRVKNVQDLMA--PRSDRSKPRYVRVNTLKLNVETALDELRKRYKV 1045
            KDA+ SALAK++V+ + KN+ D +A     D +KP YVRVNTLKL+V++AL EL K++KV
Sbjct: 126  KDAMQSALAKLVVRKKAKNIDDFIALYQPPDITKPCYVRVNTLKLDVDSALHELGKQFKV 185

Query: 1044 HKXXXXXXXXXXXPCTDLHEHPLVKNGSVFLQGKASSLVAVALDPSPGWEVLDACSAPGN 865
             K           P TDLH H LV NGS+F+QGKASS+VA  LDP PGWEVLDACSAPGN
Sbjct: 186  QKDDMIPHLLVLPPHTDLHNHSLVLNGSIFVQGKASSMVAAVLDPKPGWEVLDACSAPGN 245

Query: 864  KTVHLAALMRGKGKIIACELNKERVERLKHTIKQAGATNVEVLHKDFLKLSPVDPSYSKV 685
            KTVHLAALM+GKGKIIACELNK+R +RL+ TI+ +GA N+EVLH DFL + P  P +SKV
Sbjct: 246  KTVHLAALMKGKGKIIACELNKDRAKRLEDTIRLSGAANIEVLHGDFLNIDPKGP-FSKV 304

Query: 684  RAILLDPSCSGSGTAVERLDHLLPSYQQGHADPSDMHRLTKLSAFQKMALEHALSFPGVE 505
             AILLDPSCSGSGTA +RLDHLLPS      D  D  RL KL+AFQK AL HALSF  V+
Sbjct: 305  SAILLDPSCSGSGTAAQRLDHLLPSRT---TDVVDTERLNKLAAFQKKALAHALSFTAVK 361

Query: 504  RIVYSTCSIHQIENEDVIKSVLPLATSCGFQLSTPFPQWPRRGLPVLEEFKHLLRTDPVE 325
            RIVYSTCSI+QIENEDV+ S+LPLATS GFQL+TPFPQW RRGLPV E  +HLLRTDPVE
Sbjct: 362  RIVYSTCSINQIENEDVVNSILPLATSNGFQLATPFPQWQRRGLPVFEGSEHLLRTDPVE 421

Query: 324  DKEGFFIALFVR 289
            DKEGFFIALFV+
Sbjct: 422  DKEGFFIALFVK 433