BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00021065
         (486 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACB05475.1| LIM domain protein 2b [Nicotiana tabacum]              286   1e-75
gb|AAF75828.1|AF116851_1 LIM domain protein PLIM-2 [Nicotiana ta...   278   3e-73
ref|XP_002269537.1| PREDICTED: pollen-specific protein SF3 [Viti...   275   2e-72
ref|XP_002320783.1| predicted protein [Populus trichocarpa] gi|2...   272   2e-71
ref|XP_002302574.1| predicted protein [Populus trichocarpa] gi|2...   269   2e-70

>gb|ACB05475.1| LIM domain protein 2b [Nicotiana tabacum]
          Length = 216

 Score =  286 bits (732), Expect = 1e-75
 Identities = 126/153 (82%), Positives = 143/153 (93%), Gaps = 1/153 (0%)
 Frame = -2

Query: 458 SGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKPHFE 279
           +GTLDKCKACDKTVYFVDLL+ DG  YHKSCFKCSHCKGTL MS+YSSMDG LYCKPHFE
Sbjct: 4   TGTLDKCKACDKTVYFVDLLTADGITYHKSCFKCSHCKGTLVMSNYSSMDGVLYCKPHFE 63

Query: 278 QLFKESGNFSKNFHTS-KPERENSITKTPNKLSSMFSGTQDKCAACHKTVYPFEKITMEG 102
           QLFKE GNFSKNF TS KPERE+++T+TP+KLS+MFSGTQDKCAAC+KTVYP EK+TMEG
Sbjct: 64  QLFKECGNFSKNFQTSAKPEREHALTRTPSKLSAMFSGTQDKCAACNKTVYPLEKVTMEG 123

Query: 101 ESYHRPCFKCAHGGCPLTHSSYAALDGILYCKH 3
           ES+H+ CFKCAHGGCPLTH++YA+LDG+LYCKH
Sbjct: 124 ESFHKSCFKCAHGGCPLTHATYASLDGVLYCKH 156



 Score = 92.8 bits (229), Expect = 3e-17
 Identities = 41/90 (45%), Positives = 60/90 (66%)
 Frame = -2

Query: 458 SGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKPHFE 279
           SGT DKC AC+KTVY ++ ++++G  +HKSCFKC+H    L  ++Y+S+DG LYCK HF 
Sbjct: 100 SGTQDKCAACNKTVYPLEKVTMEGESFHKSCFKCAHGGCPLTHATYASLDGVLYCKHHFA 159

Query: 278 QLFKESGNFSKNFHTSKPERENSITKTPNK 189
           QLF E G +      +  ++ N+ T T N+
Sbjct: 160 QLFMEKGTYQHVLEAANNKKINAETPTNNE 189



 Score = 73.2 bits (178), Expect = 2e-11
 Identities = 30/57 (52%), Positives = 42/57 (73%)
 Frame = -2

Query: 176 FSGTQDKCAACHKTVYPFEKITMEGESYHRPCFKCAHGGCPLTHSSYAALDGILYCK 6
           F+GT DKC AC KTVY  + +T +G +YH+ CFKC+H    L  S+Y+++DG+LYCK
Sbjct: 3   FTGTLDKCKACDKTVYFVDLLTADGITYHKSCFKCSHCKGTLVMSNYSSMDGVLYCK 59


>gb|AAF75828.1|AF116851_1 LIM domain protein PLIM-2 [Nicotiana tabacum]
          Length = 212

 Score =  278 bits (711), Expect = 3e-73
 Identities = 122/152 (80%), Positives = 137/152 (90%)
 Frame = -2

Query: 458 SGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKPHFE 279
           +GTLDKC ACDKTVYFVDLLS DG  YHKSCFKCSHCKGTL MS+YSSM+G LYCK HFE
Sbjct: 4   TGTLDKCSACDKTVYFVDLLSADGVTYHKSCFKCSHCKGTLVMSNYSSMEGVLYCKHHFE 63

Query: 278 QLFKESGNFSKNFHTSKPERENSITKTPNKLSSMFSGTQDKCAACHKTVYPFEKITMEGE 99
           QLFKESGNF+KNF  SK ER+NS+T+ P+KLS+MFSGTQDKCAAC KTVYP EK+TMEGE
Sbjct: 64  QLFKESGNFTKNFQNSKAERQNSLTRAPSKLSAMFSGTQDKCAACDKTVYPLEKVTMEGE 123

Query: 98  SYHRPCFKCAHGGCPLTHSSYAALDGILYCKH 3
           S+H+ CFKCAHGGCPLTH++YA+LDG LYCKH
Sbjct: 124 SFHKSCFKCAHGGCPLTHATYASLDGNLYCKH 155



 Score = 92.4 bits (228), Expect = 3e-17
 Identities = 42/88 (47%), Positives = 59/88 (67%)
 Frame = -2

Query: 458 SGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKPHFE 279
           SGT DKC ACDKTVY ++ ++++G  +HKSCFKC+H    L  ++Y+S+DG LYCK HF 
Sbjct: 99  SGTQDKCAACDKTVYPLEKVTMEGESFHKSCFKCAHGGCPLTHATYASLDGNLYCKHHFA 158

Query: 278 QLFKESGNFSKNFHTSKPERENSITKTP 195
           QLF E GN+      +   +++S   TP
Sbjct: 159 QLFMEKGNYQHVLKAAN-NKKSSAAVTP 185



 Score = 74.3 bits (181), Expect = 9e-12
 Identities = 29/58 (50%), Positives = 44/58 (75%)
 Frame = -2

Query: 176 FSGTQDKCAACHKTVYPFEKITMEGESYHRPCFKCAHGGCPLTHSSYAALDGILYCKH 3
           F+GT DKC+AC KTVY  + ++ +G +YH+ CFKC+H    L  S+Y++++G+LYCKH
Sbjct: 3   FTGTLDKCSACDKTVYFVDLLSADGVTYHKSCFKCSHCKGTLVMSNYSSMEGVLYCKH 60


>ref|XP_002269537.1| PREDICTED: pollen-specific protein SF3 [Vitis vinifera]
           gi|147796476|emb|CAN74803.1| hypothetical protein
           VITISV_006290 [Vitis vinifera]
          Length = 220

 Score =  275 bits (704), Expect = 2e-72
 Identities = 124/154 (80%), Positives = 139/154 (90%), Gaps = 2/154 (1%)
 Frame = -2

Query: 458 SGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKPHFE 279
           +GTLDKCKACDKTVY VDLLS DG  YHK+CFKCSHCKGTL MS+YSSMDG LYCKPHFE
Sbjct: 4   TGTLDKCKACDKTVYVVDLLSADGASYHKTCFKCSHCKGTLVMSNYSSMDGVLYCKPHFE 63

Query: 278 QLFKESGNFSKNFHTS-KP-ERENSITKTPNKLSSMFSGTQDKCAACHKTVYPFEKITME 105
           QLFKESGNFSKNF TS KP ++ N +++ P+KLSSMFSGTQDKC+AC KTVYP EK+T+E
Sbjct: 64  QLFKESGNFSKNFQTSAKPADKLNELSRAPSKLSSMFSGTQDKCSACRKTVYPLEKVTLE 123

Query: 104 GESYHRPCFKCAHGGCPLTHSSYAALDGILYCKH 3
           GESYH+ CFKCAHGGCPLTHSSYAAL+G+LYCKH
Sbjct: 124 GESYHKSCFKCAHGGCPLTHSSYAALNGVLYCKH 157



 Score = 93.2 bits (230), Expect = 2e-17
 Identities = 41/73 (56%), Positives = 54/73 (73%)
 Frame = -2

Query: 467 SVSSGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKP 288
           S+ SGT DKC AC KTVY ++ ++++G  YHKSCFKC+H    L  SSY++++G LYCK 
Sbjct: 98  SMFSGTQDKCSACRKTVYPLEKVTLEGESYHKSCFKCAHGGCPLTHSSYAALNGVLYCKH 157

Query: 287 HFEQLFKESGNFS 249
           HF QLF E GN+S
Sbjct: 158 HFSQLFMEKGNYS 170



 Score = 74.3 bits (181), Expect = 9e-12
 Identities = 30/57 (52%), Positives = 42/57 (73%)
 Frame = -2

Query: 176 FSGTQDKCAACHKTVYPFEKITMEGESYHRPCFKCAHGGCPLTHSSYAALDGILYCK 6
           F+GT DKC AC KTVY  + ++ +G SYH+ CFKC+H    L  S+Y+++DG+LYCK
Sbjct: 3   FTGTLDKCKACDKTVYVVDLLSADGASYHKTCFKCSHCKGTLVMSNYSSMDGVLYCK 59


>ref|XP_002320783.1| predicted protein [Populus trichocarpa] gi|222861556|gb|EEE99098.1|
           predicted protein [Populus trichocarpa]
          Length = 206

 Score =  272 bits (695), Expect = 2e-71
 Identities = 117/151 (77%), Positives = 135/151 (89%)
 Frame = -2

Query: 458 SGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKPHFE 279
           +GTLDKCKACDKTVYFVD++S++G PYHKSCFKCSHCKGTL MS+YSSMDG LYCK HFE
Sbjct: 4   TGTLDKCKACDKTVYFVDMMSLEGVPYHKSCFKCSHCKGTLVMSNYSSMDGVLYCKTHFE 63

Query: 278 QLFKESGNFSKNFHTSKPERENSITKTPNKLSSMFSGTQDKCAACHKTVYPFEKITMEGE 99
           QLFKE G+FSKNF   KPER + +++ P+KLSS+F GTQDKC+AC KTVYP EK+TMEGE
Sbjct: 64  QLFKEGGDFSKNFQKGKPERTHDLSRIPSKLSSVFCGTQDKCSACGKTVYPLEKVTMEGE 123

Query: 98  SYHRPCFKCAHGGCPLTHSSYAALDGILYCK 6
            YH+ CF+CAHGGCPLTHSSYAALDG+LYCK
Sbjct: 124 CYHKTCFRCAHGGCPLTHSSYAALDGVLYCK 154



 Score = 93.2 bits (230), Expect = 2e-17
 Identities = 45/95 (47%), Positives = 63/95 (66%)
 Frame = -2

Query: 467 SVSSGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKP 288
           SV  GT DKC AC KTVY ++ ++++G  YHK+CF+C+H    L  SSY+++DG LYCK 
Sbjct: 96  SVFCGTQDKCSACGKTVYPLEKVTMEGECYHKTCFRCAHGGCPLTHSSYAALDGVLYCKV 155

Query: 287 HFEQLFKESGNFSKNFHTSKPERENSITKTPNKLS 183
           HF QLF E G +S    ++  +R NS   TP +L+
Sbjct: 156 HFAQLFMEKGTYSHVLASAAHKRSNS---TPPELA 187



 Score = 73.6 bits (179), Expect = 2e-11
 Identities = 30/57 (52%), Positives = 42/57 (73%)
 Frame = -2

Query: 176 FSGTQDKCAACHKTVYPFEKITMEGESYHRPCFKCAHGGCPLTHSSYAALDGILYCK 6
           F+GT DKC AC KTVY  + +++EG  YH+ CFKC+H    L  S+Y+++DG+LYCK
Sbjct: 3   FTGTLDKCKACDKTVYFVDMMSLEGVPYHKSCFKCSHCKGTLVMSNYSSMDGVLYCK 59


>ref|XP_002302574.1| predicted protein [Populus trichocarpa] gi|222844300|gb|EEE81847.1|
           predicted protein [Populus trichocarpa]
          Length = 206

 Score =  269 bits (688), Expect = 2e-70
 Identities = 116/151 (76%), Positives = 133/151 (88%)
 Frame = -2

Query: 458 SGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKPHFE 279
           +GTLDKCKACDKTVY VD++S++G PYHKSCFKCSHCKGTL MS+YSSMDG LYCK HFE
Sbjct: 4   TGTLDKCKACDKTVYVVDMMSLEGVPYHKSCFKCSHCKGTLVMSNYSSMDGVLYCKTHFE 63

Query: 278 QLFKESGNFSKNFHTSKPERENSITKTPNKLSSMFSGTQDKCAACHKTVYPFEKITMEGE 99
           QLFKE G+FSKNF   KPER + + +TP+KLSS+F GTQDKC+ C KTVYP EK+TMEGE
Sbjct: 64  QLFKEGGDFSKNFQKGKPERTHELIRTPSKLSSVFCGTQDKCSTCGKTVYPLEKVTMEGE 123

Query: 98  SYHRPCFKCAHGGCPLTHSSYAALDGILYCK 6
            YH+ CF+CAHGGCPLTHSSYAALDG+LYCK
Sbjct: 124 CYHKTCFRCAHGGCPLTHSSYAALDGVLYCK 154



 Score = 89.0 bits (219), Expect = 4e-16
 Identities = 47/111 (42%), Positives = 68/111 (61%)
 Frame = -2

Query: 467 SVSSGTLDKCKACDKTVYFVDLLSVDGTPYHKSCFKCSHCKGTLAMSSYSSMDGTLYCKP 288
           SV  GT DKC  C KTVY ++ ++++G  YHK+CF+C+H    L  SSY+++DG LYCK 
Sbjct: 96  SVFCGTQDKCSTCGKTVYPLEKVTMEGECYHKTCFRCAHGGCPLTHSSYAALDGVLYCKV 155

Query: 287 HFEQLFKESGNFSKNFHTSKPERENSITKTPNKLSSMFSGTQDKCAACHKT 135
           HF QLF E G +S     +  +R  S +  P +L+   S T++  AA  K+
Sbjct: 156 HFAQLFMEKGTYSHVLAGATHKR--STSTPPPELAE--SNTEEAAAAEDKS 202



 Score = 74.3 bits (181), Expect = 9e-12
 Identities = 30/57 (52%), Positives = 42/57 (73%)
 Frame = -2

Query: 176 FSGTQDKCAACHKTVYPFEKITMEGESYHRPCFKCAHGGCPLTHSSYAALDGILYCK 6
           F+GT DKC AC KTVY  + +++EG  YH+ CFKC+H    L  S+Y+++DG+LYCK
Sbjct: 3   FTGTLDKCKACDKTVYVVDMMSLEGVPYHKSCFKCSHCKGTLVMSNYSSMDGVLYCK 59