BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020772
         (1309 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAM38525.1| endonuclease [Apium graveolens]                       517   e-144
gb|AFC88012.1| PARS II endonuclease [Petroselinum crispum]            514   e-143
emb|CBI18600.3| unnamed protein product [Vitis vinifera]              429   e-117
ref|XP_002269258.1| PREDICTED: nuclease S1 [Vitis vinifera]           429   e-117
ref|XP_003608197.1| Nuclease S1 [Medicago truncatula] gi|3555092...   411   e-112

>dbj|BAM38525.1| endonuclease [Apium graveolens]
          Length = 310

 Score =  517 bits (1332), Expect = e-144
 Identities = 247/293 (84%), Positives = 258/293 (88%)
 Frame = +1

Query: 121 LSATGLFXXXXXXXXXXWGKDGHFAICKIAQGLLNKDALTGVQALLPEHADGDLAAVCSW 300
           L+ TG++          WGK GHFAICKIAQG L+KDALT V+ALLPE+ADGDLAAVCSW
Sbjct: 4   LTYTGIYFLLLLPSVFCWGKQGHFAICKIAQGFLSKDALTAVKALLPEYADGDLAAVCSW 63

Query: 301 ADEVRFHMRWSSPLHYVDTPDFRCNYRYCRDCHDSVGRKDRCVTGAIYNYTEQLLLGVHX 480
           ADEVRFHMRWSSPLHYVDTPDFRCNY+YCRDCHDSVGRKDRCVTGAI+NYTEQLLLGVH 
Sbjct: 64  ADEVRFHMRWSSPLHYVDTPDFRCNYKYCRDCHDSVGRKDRCVTGAIHNYTEQLLLGVHD 123

Query: 481 XXXXXXXXXXXALMFLSHFVGDVHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMII 660
                      ALMFLSHFVGDVHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWD M+I
Sbjct: 124 LNSKMNNNLTEALMFLSHFVGDVHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDTMMI 183

Query: 661 ESSLKTFYNSDLSSLIQAIQSNITGVWFTDSLSWENCTADHVVCPDPYASESIELACKFA 840
           ESSLKTFYNSDLSSLIQAIQSNITGVW TDSLSW NCTADHVVCPDPYASESIELACKFA
Sbjct: 184 ESSLKTFYNSDLSSLIQAIQSNITGVWLTDSLSWSNCTADHVVCPDPYASESIELACKFA 243

Query: 841 YRNATPGTTLGDDYFLSRLPVVEKRLAQAGVRLAATLNRIFTPTTIKLTRLNV 999
           YRNATPGTTLGD+YFLSRLPV EKRLAQAGVRLAATLNRIFT     LTRLN+
Sbjct: 244 YRNATPGTTLGDEYFLSRLPVAEKRLAQAGVRLAATLNRIFTSNPSDLTRLNM 296


>gb|AFC88012.1| PARS II endonuclease [Petroselinum crispum]
          Length = 309

 Score =  514 bits (1323), Expect = e-143
 Identities = 245/293 (83%), Positives = 256/293 (87%)
 Frame = +1

Query: 121 LSATGLFXXXXXXXXXXWGKDGHFAICKIAQGLLNKDALTGVQALLPEHADGDLAAVCSW 300
           L+ T ++          WGK+GHFAICKIAQG L KDALT V+ALLPE+ADGDLAAVCSW
Sbjct: 4   LTYTAIYFLLLLPSVFSWGKEGHFAICKIAQGFLTKDALTAVKALLPEYADGDLAAVCSW 63

Query: 301 ADEVRFHMRWSSPLHYVDTPDFRCNYRYCRDCHDSVGRKDRCVTGAIYNYTEQLLLGVHX 480
           ADEVRFHMRWSSPLHYVDTPDFRCNY+YCRDCHDSVGRKDRCVTGAIYNYTEQLLLGVH 
Sbjct: 64  ADEVRFHMRWSSPLHYVDTPDFRCNYKYCRDCHDSVGRKDRCVTGAIYNYTEQLLLGVHD 123

Query: 481 XXXXXXXXXXXALMFLSHFVGDVHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMII 660
                      ALMFLSHFVGDVHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWD M+I
Sbjct: 124 LDSKMNNNLTEALMFLSHFVGDVHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDTMMI 183

Query: 661 ESSLKTFYNSDLSSLIQAIQSNITGVWFTDSLSWENCTADHVVCPDPYASESIELACKFA 840
           ESSLKTFYNSDLSSLIQ+IQSNITG+W TDSLSW NCTAD V CPDPYASESIELACKFA
Sbjct: 184 ESSLKTFYNSDLSSLIQSIQSNITGIWLTDSLSWRNCTADQVACPDPYASESIELACKFA 243

Query: 841 YRNATPGTTLGDDYFLSRLPVVEKRLAQAGVRLAATLNRIFTPTTIKLTRLNV 999
           YRNATPGTTLGD+YFLSRLPVVEKRLAQAGVRLAATLNRIFT     LTRLNV
Sbjct: 244 YRNATPGTTLGDEYFLSRLPVVEKRLAQAGVRLAATLNRIFTSKPSNLTRLNV 296


>emb|CBI18600.3| unnamed protein product [Vitis vinifera]
          Length = 332

 Score =  429 bits (1102), Expect = e-117
 Identities = 197/266 (74%), Positives = 223/266 (83%), Gaps = 2/266 (0%)
 Frame = +1

Query: 172 WGKDGHFAICKIAQGLLNKDALTGVQALLPEHADGDLAAVCSWADEVR--FHMRWSSPLH 345
           WGK+GH+A+CKIA+G L++DAL  V+ALLP++A+GDLAAVCSWADE+R  FH RWS PLH
Sbjct: 25  WGKEGHYAVCKIAEGFLSEDALGAVKALLPDYAEGDLAAVCSWADEIRHNFHWRWSGPLH 84

Query: 346 YVDTPDFRCNYRYCRDCHDSVGRKDRCVTGAIYNYTEQLLLGVHXXXXXXXXXXXXALMF 525
           YVDTPD+RCNY YCRDCHD  G KD CVTGAIYNYT+QL  G H            ALMF
Sbjct: 85  YVDTPDYRCNYEYCRDCHDFRGHKDICVTGAIYNYTKQLTSGYHNSGSEIRYNLTEALMF 144

Query: 526 LSHFVGDVHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESSLKTFYNSDLSSL 705
           LSHF+GDVHQPLHVGF GDEGGNTI VRWYRRKTNLHH+WDNMII+S+LKT+YNSDL+ +
Sbjct: 145 LSHFIGDVHQPLHVGFTGDEGGNTIIVRWYRRKTNLHHIWDNMIIDSALKTYYNSDLAIM 204

Query: 706 IQAIQSNITGVWFTDSLSWENCTADHVVCPDPYASESIELACKFAYRNATPGTTLGDDYF 885
           IQAIQ NITG W  D  SW+NC +D   CP+ YASESI LACKFAYRNATPG+TLGDDYF
Sbjct: 205 IQAIQRNITGDWSFDISSWKNCASDDTACPNLYASESISLACKFAYRNATPGSTLGDDYF 264

Query: 886 LSRLPVVEKRLAQAGVRLAATLNRIF 963
           LSRLP+VEKRLAQ G+RLAATLNRIF
Sbjct: 265 LSRLPIVEKRLAQGGIRLAATLNRIF 290


>ref|XP_002269258.1| PREDICTED: nuclease S1 [Vitis vinifera]
          Length = 323

 Score =  429 bits (1102), Expect = e-117
 Identities = 197/266 (74%), Positives = 223/266 (83%), Gaps = 2/266 (0%)
 Frame = +1

Query: 172 WGKDGHFAICKIAQGLLNKDALTGVQALLPEHADGDLAAVCSWADEVR--FHMRWSSPLH 345
           WGK+GH+A+CKIA+G L++DAL  V+ALLP++A+GDLAAVCSWADE+R  FH RWS PLH
Sbjct: 25  WGKEGHYAVCKIAEGFLSEDALGAVKALLPDYAEGDLAAVCSWADEIRHNFHWRWSGPLH 84

Query: 346 YVDTPDFRCNYRYCRDCHDSVGRKDRCVTGAIYNYTEQLLLGVHXXXXXXXXXXXXALMF 525
           YVDTPD+RCNY YCRDCHD  G KD CVTGAIYNYT+QL  G H            ALMF
Sbjct: 85  YVDTPDYRCNYEYCRDCHDFRGHKDICVTGAIYNYTKQLTSGYHNSGSEIRYNLTEALMF 144

Query: 526 LSHFVGDVHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESSLKTFYNSDLSSL 705
           LSHF+GDVHQPLHVGF GDEGGNTI VRWYRRKTNLHH+WDNMII+S+LKT+YNSDL+ +
Sbjct: 145 LSHFIGDVHQPLHVGFTGDEGGNTIIVRWYRRKTNLHHIWDNMIIDSALKTYYNSDLAIM 204

Query: 706 IQAIQSNITGVWFTDSLSWENCTADHVVCPDPYASESIELACKFAYRNATPGTTLGDDYF 885
           IQAIQ NITG W  D  SW+NC +D   CP+ YASESI LACKFAYRNATPG+TLGDDYF
Sbjct: 205 IQAIQRNITGDWSFDISSWKNCASDDTACPNLYASESISLACKFAYRNATPGSTLGDDYF 264

Query: 886 LSRLPVVEKRLAQAGVRLAATLNRIF 963
           LSRLP+VEKRLAQ G+RLAATLNRIF
Sbjct: 265 LSRLPIVEKRLAQGGIRLAATLNRIF 290


>ref|XP_003608197.1| Nuclease S1 [Medicago truncatula] gi|355509252|gb|AES90394.1|
           Nuclease S1 [Medicago truncatula]
          Length = 383

 Score =  411 bits (1056), Expect = e-112
 Identities = 193/270 (71%), Positives = 216/270 (80%), Gaps = 2/270 (0%)
 Frame = +1

Query: 172 WGKDGHFAICKIAQGLLNKDALTGVQALLPEHADGDLAAVCSWADEVR--FHMRWSSPLH 345
           WGKDGH+AICKI+Q  L++DAL  V+ LLP+ A  DLA+VCSW DE+R  +H RWSSPLH
Sbjct: 109 WGKDGHYAICKISQEYLSEDALFAVKQLLPDSAQADLASVCSWPDEIRHNYHYRWSSPLH 168

Query: 346 YVDTPDFRCNYRYCRDCHDSVGRKDRCVTGAIYNYTEQLLLGVHXXXXXXXXXXXXALMF 525
           Y+DTPDF+CNY+YCRDCHDS G K RCVTGAIYNYT QL L               ALMF
Sbjct: 169 YIDTPDFKCNYQYCRDCHDSYGHKHRCVTGAIYNYTMQLKLANADASSELKYNLTEALMF 228

Query: 526 LSHFVGDVHQPLHVGFLGDEGGNTITVRWYRRKTNLHHVWDNMIIESSLKTFYNSDLSSL 705
           LSHFVGDVHQPLHVGF GD GGN+ITVRWYRRKTNLHHVWDNMIIES+LK FY SDLS++
Sbjct: 229 LSHFVGDVHQPLHVGFTGDLGGNSITVRWYRRKTNLHHVWDNMIIESALKKFYGSDLSTM 288

Query: 706 IQAIQSNITGVWFTDSLSWENCTADHVVCPDPYASESIELACKFAYRNATPGTTLGDDYF 885
           IQAIQ NI+ +W  D   WE+C  +H  CPD YASESI LACKFAY+NATPG+TL DDYF
Sbjct: 289 IQAIQRNISDIWSNDVSIWEHCAHNHTACPDRYASESISLACKFAYKNATPGSTLEDDYF 348

Query: 886 LSRLPVVEKRLAQAGVRLAATLNRIFTPTT 975
           LSRLP+VEKRLAQ GVRLAA LN IFTP T
Sbjct: 349 LSRLPIVEKRLAQGGVRLAAILNHIFTPKT 378