BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020744
         (1117 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABQ52488.1| GA 20-oxidase [Paeonia suffruticosa]                   471   e-130
ref|XP_002307041.1| gibberellin 20-oxidase [Populus trichocarpa]...   454   e-125
ref|XP_002527163.1| gibberellin 20-oxidase, putative [Ricinus co...   452   e-125
ref|XP_002310523.1| gibberellin 20-oxidase [Populus trichocarpa]...   452   e-125
ref|XP_003526870.1| PREDICTED: LOW QUALITY PROTEIN: gibberellin ...   442   e-122

>gb|ABQ52488.1| GA 20-oxidase [Paeonia suffruticosa]
          Length = 381

 Score =  471 bits (1211), Expect = e-130
 Identities = 225/362 (62%), Positives = 286/362 (79%)
 Frame = +1

Query: 13   DLSSPTLIYNPSSFKNIQPTQFLWPKDELVNEVYEDLNEPLIDLAGFFSGNTGETSKAVE 192
            D  +  L+++    +   PT+F+WP+ +LV    E+L EPL+DLAGF++G+   T+ A E
Sbjct: 23   DRENGVLLFDSLQKQANLPTEFVWPQGDLVRTQEEELKEPLVDLAGFYTGDELATTHAAE 82

Query: 193  LLHTACVNHGFFQVTNHGVEEWLIAAAHHELDALFKLSNENKMSLKRKAGDISGYSGAHS 372
            L+ TAC+NHGFFQV NHG++  LI+AAH E++  FKL    K+S++RK G + GYSGAHS
Sbjct: 83   LIRTACLNHGFFQVINHGIDANLISAAHDEMNVFFKLPISKKLSVRRKPGGVWGYSGAHS 142

Query: 373  DRYSTKLPWKETFSFRYDYGSYATKSMVLDYFNSVIGPEFEEAGNVYQWYCEALEKLALA 552
            DRY++KLPWKET SF Y  GS A+  +V+DYF S +G +FE  G VYQ YCEA+++L+L 
Sbjct: 143  DRYTSKLPWKETLSFGYQ-GS-ASHPVVVDYFKSSLGKDFEHTGWVYQSYCEAMKELSLV 200

Query: 553  IFELLAISLGVEQDYFKKFFEDGTSIMRCNYYPKCNEPELTLGTGPHTDPTALTILHQDQ 732
            I ELLAISLGV++ Y++K+F+DG+SIMRCNYYP CNEP LTLGTGPH DPT++TILHQDQ
Sbjct: 201  IMELLAISLGVDRLYYRKYFKDGSSIMRCNYYPPCNEPGLTLGTGPHCDPTSITILHQDQ 260

Query: 733  VGGLQVFTNNKWQSIRPRPDAFVINIGDTFTALSNGTYKSCVHRAVVNKESARKSLVYFV 912
            VGGL+VF NNKWQ +RPRPDAFVINIGDTF ALSNG YKSC+HRAVVN+ES R+SLV+FV
Sbjct: 261  VGGLEVFANNKWQGVRPRPDAFVINIGDTFMALSNGKYKSCLHRAVVNRESERRSLVFFV 320

Query: 913  CPREDKVIRADEDLVEKKCRTRKYPDYIWSDLFTFTQTHYRADNSTLFAFFTWLLSSPTT 1092
            CP+EDKV+R  +DLV ++   RKYPD+ WSDLF FTQ HYRAD +TL +FF WLLSS  +
Sbjct: 321  CPKEDKVVRPPQDLVGREV-PRKYPDFTWSDLFEFTQKHYRADVTTLQSFFQWLLSSKPS 379

Query: 1093 *F 1098
             F
Sbjct: 380  KF 381


>ref|XP_002307041.1| gibberellin 20-oxidase [Populus trichocarpa]
            gi|222856490|gb|EEE94037.1| gibberellin 20-oxidase
            [Populus trichocarpa]
          Length = 379

 Score =  454 bits (1168), Expect = e-125
 Identities = 228/379 (60%), Positives = 284/379 (74%), Gaps = 20/379 (5%)
 Frame = +1

Query: 7    VMDLSSPTLIYNP---------------SSFKNIQ---PTQFLWPKDELVNEVYEDLNEP 132
            +MD +S +L+ +P               SSF   Q   PT+F+WP  +LV+   ++L EP
Sbjct: 4    LMDSTSSSLLLSPPPFHSKDETDAPVLYSSFLQKQANMPTEFIWPIGDLVHN-QDELKEP 62

Query: 133  LIDLAGFFSGNTGETSKAVELLHTACVNHGFFQVTNHGVEEWLIAAAHHELDALFKLSNE 312
            LIDL GF  G+   T+ A EL+ TAC+NHGFFQV NHGV+  LI AAH E+D +FKL  +
Sbjct: 63   LIDLDGFLKGDERATADAAELVRTACLNHGFFQVINHGVDIGLIHAAHEEIDKIFKLPLD 122

Query: 313  NKMSLKRKAGDISGYSGAHSDRYSTKLPWKETFSFRYDYGSYATKSMVLDYFNSVIGPEF 492
             K+S +RK GD+SGYSGAH+ RYS+KLPWKETFSF Y +G   ++ +V+DYF SV+G  F
Sbjct: 123  KKLSTRRKPGDVSGYSGAHAHRYSSKLPWKETFSFGY-HGDDDSEPLVVDYFKSVLGENF 181

Query: 493  EEAGNVYQWYCEALEKLALAIFELLAISLGVEQDYFKKFFEDGTSIMRCNYYPKCNEPEL 672
            E  G VYQ YCEA++K++L IFELL ISLGV++ +++KFFEDG+SIMRCN YP CN   L
Sbjct: 182  EHTGWVYQRYCEAMKKVSLVIFELLGISLGVDRLHYRKFFEDGSSIMRCNNYPPCNNSSL 241

Query: 673  TLGTGPHTDPTALTILHQDQVGGLQVFTNNKWQSIRPRPDAFVINIGDTFTALSNGTYKS 852
            TLGTGPH DPT+LTILHQDQVGGL+VF NNKWQ+IRPRPDA V+NIGDTFTALSNG Y+S
Sbjct: 242  TLGTGPHCDPTSLTILHQDQVGGLEVFANNKWQAIRPRPDALVVNIGDTFTALSNGRYQS 301

Query: 853  CVHRAVVNKESARKSLVYFVCPREDKVIRADEDLVEKKCR--TRKYPDYIWSDLFTFTQT 1026
            C+HRAVVN+E  RKSLV+FV P+E+KV+R  +DLV   CR   RKYPD+ WSDL  FTQ 
Sbjct: 302  CLHRAVVNRERERKSLVFFVSPKEEKVVRPPQDLV---CREGPRKYPDFTWSDLLEFTQK 358

Query: 1027 HYRADNSTLFAFFTWLLSS 1083
            HYRAD +TL +F  WLLS+
Sbjct: 359  HYRADVATLQSFIQWLLSA 377


>ref|XP_002527163.1| gibberellin 20-oxidase, putative [Ricinus communis]
            gi|223533472|gb|EEF35218.1| gibberellin 20-oxidase,
            putative [Ricinus communis]
          Length = 387

 Score =  452 bits (1163), Expect = e-125
 Identities = 214/346 (61%), Positives = 270/346 (78%), Gaps = 2/346 (0%)
 Frame = +1

Query: 67   PTQFLWPKDELVNEVYEDLNEPLIDLAGFFSGNTGETSKAVELLHTACVNHGFFQVTNHG 246
            PT+F+WP  +LV+   ++L EPLIDL G   G+  ET+ A EL+  AC+NHGFFQVTNHG
Sbjct: 48   PTEFIWPNGDLVSN-QDELKEPLIDLDGILKGDEVETAHAAELVRAACLNHGFFQVTNHG 106

Query: 247  VEEWLIAAAHHELDALFKLSNENKMSLKRKAGDISGYSGAHSDRYSTKLPWKETFSFRYD 426
            V+  LI AAH E+D +FKL  + K+S++R  G + GYSGAH+DRYS KLPWKETF+F Y 
Sbjct: 107  VDASLIRAAHEEVDTIFKLPLDKKLSVRRMPGSVYGYSGAHADRYSFKLPWKETFTFGYS 166

Query: 427  YGSYATKSMVLDYFNSVIGPEFEEAGNVYQWYCEALEKLALAIFELLAISLGVEQDYFKK 606
                 ++ +V+DYF  V+G ++E  G +YQ +CE ++KL+  IFELLAISLGV++ +++K
Sbjct: 167  RSD--SEPIVVDYFKHVLGEDYERTGGIYQKFCEEMKKLSFVIFELLAISLGVDRFHYRK 224

Query: 607  FFEDGTSIMRCNYYPKCNEPELTLGTGPHTDPTALTILHQDQVGGLQVFTNNKWQSIRPR 786
            +FEDG+SIMRCNYYP CN   LTLGTGPH DPT+LTILHQD+VGGL+VF N+KWQ+IRPR
Sbjct: 225  YFEDGSSIMRCNYYPACNNSSLTLGTGPHCDPTSLTILHQDEVGGLEVFANSKWQAIRPR 284

Query: 787  PDAFVINIGDTFTALSNGTYKSCVHRAVVNKESARKSLVYFVCPREDKVIRADEDLVEKK 966
            PDAFVINIGDTFTALSNG YKSC+HRAV+N+E  R+SLV+FVCP+EDKV+R  +DLV   
Sbjct: 285  PDAFVINIGDTFTALSNGRYKSCLHRAVLNRERERRSLVFFVCPKEDKVVRPPQDLV--- 341

Query: 967  CR--TRKYPDYIWSDLFTFTQTHYRADNSTLFAFFTWLLSSPTT*F 1098
            CR   RKYPD+ WSDL  FTQ HYRAD +TL +F  WLLSS  + F
Sbjct: 342  CREGPRKYPDFTWSDLLEFTQKHYRADAATLQSFIQWLLSSKPSNF 387


>ref|XP_002310523.1| gibberellin 20-oxidase [Populus trichocarpa]
            gi|222853426|gb|EEE90973.1| gibberellin 20-oxidase
            [Populus trichocarpa]
          Length = 378

 Score =  452 bits (1162), Expect = e-125
 Identities = 217/343 (63%), Positives = 276/343 (80%), Gaps = 1/343 (0%)
 Frame = +1

Query: 67   PTQFLWPKDELVNEVYEDLNEPLIDLAGFFSGNTGETSKAVELLHTACVNHGFFQVTNHG 246
            PT+F+WP  +LV+   ++L EP+IDL GF  G+   T++A EL+ TAC+NHGFFQVTNHG
Sbjct: 38   PTEFMWPHGDLVHNE-DELKEPMIDLEGFLKGDEVATAQAAELVRTACLNHGFFQVTNHG 96

Query: 247  VEEWLIAAAHHELDALFKLSNENKMSLKRKAGDISGYSGAHSDRYSTKLPWKETFSFRYD 426
            V+  LI +AH E+  +FKL  + K+S++RK GD+SGYSGAH+ RYS+KLPWKETFSF Y 
Sbjct: 97   VDISLIHSAHEEIGKIFKLPLDKKLSVRRKPGDVSGYSGAHAHRYSSKLPWKETFSFGY- 155

Query: 427  YGSYATKSMVLDYFNSVIGPEFEEAGNVYQWYCEALEKLALAIFELLAISLGVEQDYFKK 606
            +G   +  +V+DYF SV+G +FE  G VYQ YCEA++K++L IFELLAISLGV++ +++K
Sbjct: 156  HGDDDSVPLVVDYFKSVLGKDFEHTGWVYQSYCEAMKKVSLVIFELLAISLGVDRLHYRK 215

Query: 607  FFEDGTSIMRCNYYPKCNEPELTLGTGPHTDPTALTILHQDQVGGLQVFTNNKWQSIRPR 786
            FFEDG+SIMRCNYYP CN   LTLGTGPH DPT+LTILHQDQVGGLQVF+NNKW +IRPR
Sbjct: 216  FFEDGSSIMRCNYYPPCNNSTLTLGTGPHCDPTSLTILHQDQVGGLQVFSNNKWLAIRPR 275

Query: 787  PDAFVINIGDTFTALSNGTYKSCVHRAVVNKESARKSLVYFVCPREDKVIRADEDLVEKK 966
            PDA V+NIGDTF ALSNG YKSC+HRAVVN++S R+SLV+FV P+E+KV+R  +DLV ++
Sbjct: 276  PDALVVNIGDTFMALSNGRYKSCLHRAVVNRDSERRSLVFFVSPKEEKVVRPPQDLVSRE 335

Query: 967  CRTRKYPDYIWSDLFTFTQTHYRADNSTLFAFFTWLLSS-PTT 1092
             + R YPD+ WSDL  FTQ HYRAD +TL +F  WL SS P+T
Sbjct: 336  GQ-RIYPDFTWSDLLEFTQKHYRADVATLQSFIQWLSSSKPST 377


>ref|XP_003526870.1| PREDICTED: LOW QUALITY PROTEIN: gibberellin 20 oxidase 3-like
            [Glycine max]
          Length = 391

 Score =  442 bits (1138), Expect = e-122
 Identities = 219/391 (56%), Positives = 280/391 (71%), Gaps = 25/391 (6%)
 Frame = +1

Query: 7    VMDLSSPTLIYNPSS-----------------FKNIQ------PTQFLWPKDELVNEVYE 117
            +M+LS+ TL+  P+S                  KN+Q      P +FLWP  +LV+   E
Sbjct: 5    LMELSASTLVLYPASQPPQEPKNENNGGIVFDSKNLQQMQGEMPKEFLWPSRDLVDTTQE 64

Query: 118  DLNEPLIDLAGFFSGNTGETSKAVELLHTACVNHGFFQVTNHGVEEWLIAAAHHELDALF 297
            +L EPL+DLA F +G+    S A EL+  AC+ HGFFQV NHGV+  LI AA+HE+D++F
Sbjct: 65   ELKEPLVDLAIFKNGDEKAISNAAELVRKACLKHGFFQVINHGVDPDLIDAAYHEIDSIF 124

Query: 298  KLSNENKMSLKRKAGDISGYSGAHSDRYSTKLPWKETFSFRYDYGSYATKSMVLDYFNSV 477
            KL    KM  KRK G +SGYSGAH+DRYS+KLPWKETFSF YD+ S++  S ++DYF SV
Sbjct: 125  KLPLSKKMGAKRKPGGVSGYSGAHADRYSSKLPWKETFSFLYDHQSFSN-SQIVDYFKSV 183

Query: 478  IGPEFEEAGNVYQWYCEALEKLALAIFELLAISLGVEQDYFKKFFEDGTSIMRCNYYPKC 657
            +G + +  G VYQ YCEA++ L+L I ELL ISLGV+++++++FF DG SIMRCNYYP C
Sbjct: 184  LGEDLQHTGRVYQKYCEAMKDLSLVIMELLGISLGVDREHYRRFFXDGDSIMRCNYYPPC 243

Query: 658  NEPELTLGTGPHTDPTALTILHQDQVGGLQVFTNNKWQSIRPRPDAFVINIGDTFTALSN 837
            N   LTLGTGPHTDPT+LTILHQDQVGGL+VF +NKW ++RPR +A VINIGDTF ALSN
Sbjct: 244  NRANLTLGTGPHTDPTSLTILHQDQVGGLEVFVDNKWLAVRPRSEALVINIGDTFMALSN 303

Query: 838  GTYKSCVHRAVVNKESARKSLVYFVCPREDKVIRADEDLVEKKCRT--RKYPDYIWSDLF 1011
            G YKSC+HRA+VN    R+SLVYFVCPREDK++R  ++L+   CR   RKYPD+ WS+LF
Sbjct: 304  GRYKSCLHRALVNTYRERRSLVYFVCPREDKIVRPPDNLL---CRNEERKYPDFTWSNLF 360

Query: 1012 TFTQTHYRADNSTLFAFFTWLLSSPTT*FNY 1104
             FTQ HYRAD +TL +F  W   S +   N+
Sbjct: 361  EFTQKHYRADVATLQSFIEWQQCSNSNPSNF 391