BLASTX nr result
ID: Bupleurum21_contig00020706
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00020706
(1522 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002283806.2| PREDICTED: uncharacterized protein LOC100243... 545 e-174
ref|XP_002318192.1| predicted protein [Populus trichocarpa] gi|2... 534 e-167
emb|CBI20080.3| unnamed protein product [Vitis vinifera] 504 e-162
ref|XP_002511058.1| tyrosyl-DNA phosphodiesterase, putative [Ric... 489 e-158
ref|XP_003630434.1| Tyrosyl-DNA phosphodiesterase [Medicago trun... 461 e-143
>ref|XP_002283806.2| PREDICTED: uncharacterized protein LOC100243589 [Vitis vinifera]
Length = 1091
Score = 545 bits (1403), Expect(2) = e-174
Identities = 266/435 (61%), Positives = 332/435 (76%), Gaps = 4/435 (0%)
Frame = +2
Query: 230 NQAHWIMELTKYDFRGAVAYLVTSVPGVYSPKIPYISDSRHYLNGPSYVSGLCGMKLLGS 409
+QAHWIMELTKYDF+GA +LV SVPG++ + P+ S S +L+ S G MK LGS
Sbjct: 567 SQAHWIMELTKYDFKGATGHLVASVPGIHFHRTPHASKSMQFLHNASCSFG---MKFLGS 623
Query: 410 IEASVVGLSHLFRASEDSSGSRIKKLAVFLRKCHENAYGMSEIVLKRNMNILADENAIAV 589
IEAS+VGLSHLF + D++G+ +KKLA FL KCH+N YGMSEIVL+RN NI AD NA+++
Sbjct: 624 IEASIVGLSHLFHTAADANGAHLKKLAAFLGKCHKNEYGMSEIVLRRNSNIPADSNAVSI 683
Query: 590 LIPKPDKFDEGDCIQLGFLPRNIAKWVAPLSDAGLFSFSAYIYRKDVLTSALEGSNKKLT 769
L+P+P + EGDCIQLGFLPR++AKWV+PL D+G F FS Y+ + L AL G K+
Sbjct: 684 LVPEPVEHSEGDCIQLGFLPRDVAKWVSPLWDSGFFRFSGYVCPMEALAVALGGKTHKVQ 743
Query: 770 LILYVSQGPSFADMSGVTQPSLVSAICSLIAVIQRSSGLWRLQEVLGHYRWPETLETDFI 949
LILYVSQG SF+ + + QP +SAICSL+A ++R GLWRLQE+LG Y+WPE+ ETDFI
Sbjct: 744 LILYVSQGASFSYILKMMQPEHLSAICSLVASLKRCVGLWRLQEILGGYQWPESQETDFI 803
Query: 950 FGSSSIG-SVNAQFLAAFSAAVGKRSMQLSESEESDPDWGCWSISQESRNPSIKIIYPTI 1126
+GSSSIG S+NAQFLAAFSAA GKRS+Q ES+ESDP+WGCWS SQESR+PSIKI++PTI
Sbjct: 804 YGSSSIGSSINAQFLAAFSAAAGKRSLQFFESDESDPEWGCWSASQESRSPSIKIVFPTI 863
Query: 1127 ERVKHASCGILASKYLLCFSEKTWLRLRNVGILHDAIPVPIERVGHPMHVKVGLRRFQSK 1306
ERVK++SCGIL S+ +LCFSEKTW RL+NV +HDAIP P +RV HPMHVKV RRFQSK
Sbjct: 864 ERVKNSSCGILPSRRILCFSEKTWQRLKNVSTIHDAIPHPSDRVKHPMHVKVAQRRFQSK 923
Query: 1307 TSASSCGWVYCGSHNFSAAAWGRPLRYK---RPGGVGRINDVLGSRLHISNYEVGVVFIV 1477
+SS GWVYCGSHNFSAAAWGRP+ + G + + RLHI NYE+G++FI
Sbjct: 924 MDSSSFGWVYCGSHNFSAAAWGRPISNPFGIKTTGTHKADSCFDQRLHICNYELGIIFIF 983
Query: 1478 PPPDTLDHVHKPKAN 1522
PP T + ++ N
Sbjct: 984 PPSATKGNGNQSSTN 998
Score = 94.7 bits (234), Expect(2) = e-174
Identities = 43/57 (75%), Positives = 49/57 (85%)
Frame = +3
Query: 60 WLFVTNTVWWQDFPRLSTPDNSSLFTQSTDGEVNQDARSDFAAQLAKFIASLVVDVP 230
W VTNTVWWQDFPR+S PD SS+FTQ DGE+N D+RSDFAAQLA F+ASLV+DVP
Sbjct: 510 WNSVTNTVWWQDFPRISPPDYSSIFTQFCDGEINLDSRSDFAAQLAGFMASLVIDVP 566
>ref|XP_002318192.1| predicted protein [Populus trichocarpa] gi|222858865|gb|EEE96412.1|
predicted protein [Populus trichocarpa]
Length = 1131
Score = 534 bits (1376), Expect(2) = e-167
Identities = 266/436 (61%), Positives = 327/436 (75%), Gaps = 5/436 (1%)
Frame = +2
Query: 230 NQAHWIMELTKYDFRGAVAYLVTSVPGVYSPKIPYISDSRHYLNGPSYVSGLCGMKLLGS 409
+QA+WI ELTKYDF GA +LV SVPG++S + P N SG G++ LGS
Sbjct: 627 SQAYWISELTKYDFEGANGHLVASVPGIHSRRSP---------NAYQLPSGSSGVQFLGS 677
Query: 410 IEASVVGLSHLFRASEDSSGSRIKKLAVFLRKCHENAYGMSEIVLKRNMNILADENAIAV 589
+EASVVGLSHLF + D +G+++K+LA FL KC EN YGMSEIVL+RN+N+ AD NA+++
Sbjct: 678 VEASVVGLSHLFHTAADRNGTQLKQLAAFLGKCCENVYGMSEIVLRRNLNVPADVNAVSI 737
Query: 590 LIPKPDKFDEGDCIQLGFLPRNIAKWVAPLSDAGLFSFSAYIYRKDVLTSALEGSNKKLT 769
L+P PD+F EGDCIQLGFLPRN+AKWV+PL D+G F FS Y+Y K+ L +AL GSN+K
Sbjct: 738 LVPNPDQFSEGDCIQLGFLPRNVAKWVSPLWDSGFFRFSGYVYPKEALAAALGGSNRK-- 795
Query: 770 LILYVSQGPSFADMSGVTQPSLVSAICSLIAVIQRSSGLWRLQEVLGHYRWPETLETDFI 949
GP F +M + Q V A CSL+A IQR +G+WRL+EVLG Y+WP++ ++DFI
Sbjct: 796 -------GPCFPNMMSLMQTEHVLAFCSLVASIQRCTGIWRLEEVLGQYKWPDSQQSDFI 848
Query: 950 FGSSSIGSVNAQFLAAFSAAVGKRSMQLSESEESDPDWGCWSISQESRNPSIKIIYPTIE 1129
+GSSSIGSVNAQFLAAFSAA GKRS +L +SEESDP+WGCWS SQE RNPSIKII+PTIE
Sbjct: 849 YGSSSIGSVNAQFLAAFSAAAGKRSPELFDSEESDPEWGCWSASQELRNPSIKIIFPTIE 908
Query: 1130 RVKHASCGILASKYLLCFSEKTWLRLRNVGILHDAIPVPIERVGHPMHVKVGLRRFQSKT 1309
RVK+A GI S+ +LCFSEKTW RLR+VGILHDAIP P +RVG PMHVKV RRFQSKT
Sbjct: 909 RVKNACNGISPSRRILCFSEKTWQRLRSVGILHDAIPHPYDRVGQPMHVKVARRRFQSKT 968
Query: 1310 SASSCGWVYCGSHNFSAAAWGRPLRYKRPGGV-----GRINDVLGSRLHISNYEVGVVFI 1474
+ASS GWVYCGSHNFSAAAWGR + P G+ G+ N L SRLH+SNYE+G++F
Sbjct: 969 NASSFGWVYCGSHNFSAAAWGRLI--SNPFGLKSKETGKTNTYLSSRLHVSNYELGIIFT 1026
Query: 1475 VPPPDTLDHVHKPKAN 1522
PP +T +K N
Sbjct: 1027 FPPTETKGITNKDCTN 1042
Score = 81.6 bits (200), Expect(2) = e-167
Identities = 37/57 (64%), Positives = 45/57 (78%)
Frame = +3
Query: 60 WLFVTNTVWWQDFPRLSTPDNSSLFTQSTDGEVNQDARSDFAAQLAKFIASLVVDVP 230
W VTNTVWWQDFP S PD S LF + +DG+ N+D+RSDFAAQLA F+A LV++VP
Sbjct: 570 WNNVTNTVWWQDFPARSAPDPSPLFIRVSDGDANKDSRSDFAAQLAGFMACLVINVP 626
>emb|CBI20080.3| unnamed protein product [Vitis vinifera]
Length = 1032
Score = 504 bits (1298), Expect(2) = e-162
Identities = 250/435 (57%), Positives = 314/435 (72%), Gaps = 4/435 (0%)
Frame = +2
Query: 230 NQAHWIMELTKYDFRGAVAYLVTSVPGVYSPKIPYISDSRHYLNGPSYVSGLCGMKLLGS 409
+QAHWIMELTKYDF+GA +LV SVPG++ + P+ S S +L+ S C
Sbjct: 527 SQAHWIMELTKYDFKGATGHLVASVPGIHFHRTPHASKSMQFLHANQNAS--CSFA---- 580
Query: 410 IEASVVGLSHLFRASEDSSGSRIKKLAVFLRKCHENAYGMSEIVLKRNMNILADENAIAV 589
D++G+ +KKLA FL KCH+N YGMSEIVL+RN NI AD NA+++
Sbjct: 581 ----------------DANGAHLKKLAAFLGKCHKNEYGMSEIVLRRNSNIPADSNAVSI 624
Query: 590 LIPKPDKFDEGDCIQLGFLPRNIAKWVAPLSDAGLFSFSAYIYRKDVLTSALEGSNKKLT 769
L+P+P + EGDCIQLGFLPR++AKWV+PL D+G F FS Y+ + L AL G K+
Sbjct: 625 LVPEPVEHSEGDCIQLGFLPRDVAKWVSPLWDSGFFRFSGYVCPMEALAVALGGKTHKVQ 684
Query: 770 LILYVSQGPSFADMSGVTQPSLVSAICSLIAVIQRSSGLWRLQEVLGHYRWPETLETDFI 949
LILYVSQG SF+ + + QP +SAICSL+A ++R GLWRLQE+LG Y+WPE+ ETDFI
Sbjct: 685 LILYVSQGASFSYILKMMQPEHLSAICSLVASLKRCVGLWRLQEILGGYQWPESQETDFI 744
Query: 950 FGSSSIGS-VNAQFLAAFSAAVGKRSMQLSESEESDPDWGCWSISQESRNPSIKIIYPTI 1126
+GSSSIGS +NAQFLAAFSAA GKRS+Q ES+ESDP+WGCWS SQESR+PSIKI++PTI
Sbjct: 745 YGSSSIGSSINAQFLAAFSAAAGKRSLQFFESDESDPEWGCWSASQESRSPSIKIVFPTI 804
Query: 1127 ERVKHASCGILASKYLLCFSEKTWLRLRNVGILHDAIPVPIERVGHPMHVKVGLRRFQSK 1306
ERVK++SCGIL S+ +LCFSEKTW RL+NV +HDAIP P +RV HPMHVKV RRFQSK
Sbjct: 805 ERVKNSSCGILPSRRILCFSEKTWQRLKNVSTIHDAIPHPSDRVKHPMHVKVAQRRFQSK 864
Query: 1307 TSASSCGWVYCGSHNFSAAAWGRPLRYK---RPGGVGRINDVLGSRLHISNYEVGVVFIV 1477
+SS GWVYCGSHNFSAAAWGRP+ + G + + RLHI NYE+G++FI
Sbjct: 865 MDSSSFGWVYCGSHNFSAAAWGRPISNPFGIKTTGTHKADSCFDQRLHICNYELGIIFIF 924
Query: 1478 PPPDTLDHVHKPKAN 1522
PP T + ++ N
Sbjct: 925 PPSATKGNGNQSSTN 939
Score = 94.7 bits (234), Expect(2) = e-162
Identities = 43/57 (75%), Positives = 49/57 (85%)
Frame = +3
Query: 60 WLFVTNTVWWQDFPRLSTPDNSSLFTQSTDGEVNQDARSDFAAQLAKFIASLVVDVP 230
W VTNTVWWQDFPR+S PD SS+FTQ DGE+N D+RSDFAAQLA F+ASLV+DVP
Sbjct: 470 WNSVTNTVWWQDFPRISPPDYSSIFTQFCDGEINLDSRSDFAAQLAGFMASLVIDVP 526
>ref|XP_002511058.1| tyrosyl-DNA phosphodiesterase, putative [Ricinus communis]
gi|223550173|gb|EEF51660.1| tyrosyl-DNA
phosphodiesterase, putative [Ricinus communis]
Length = 1148
Score = 489 bits (1259), Expect(2) = e-158
Identities = 249/443 (56%), Positives = 313/443 (70%), Gaps = 22/443 (4%)
Frame = +2
Query: 230 NQAHWIMELTKYDFRGAVAYLVTSVPGVYSPKIPYISDSRHYLNGPSYVSGLCGMKLLGS 409
+QAHW++ELTKY+F GA+ YLV S+PG++S PY Y +K LGS
Sbjct: 623 SQAHWVVELTKYNFDGALGYLVASIPGIHSRGTPYACQ---------YAMKSIDVKFLGS 673
Query: 410 IEASVVGLSHLFRASEDSSGSRIKKLAVFLRKCHENAYGMSEIVLKRNMNILADENAIAV 589
+EASVVGLSHLF S D++G+ +KKLA FL + ENAYGMSEI+L+RN N+ AD NA+++
Sbjct: 674 VEASVVGLSHLFHTSTDTNGALLKKLAAFLGRFPENAYGMSEIILRRNTNVPADVNAVSI 733
Query: 590 LIPKPDKFDEGDCIQLGFLPRNIAKWVAPLSDAGLFSFSAYIYRKDVLTSALEGSNKKLT 769
LIP PDKF GDC+QLGFLPR +AKWV+PL D+G F FS YI+ K+ L +A G++ +
Sbjct: 734 LIPNPDKFS-GDCVQLGFLPRYVAKWVSPLWDSGFFKFSGYIHPKEALEAASGGNDMR-- 790
Query: 770 LILYVSQGPSFADMSGVTQPSLVSAICSLIAVIQRSSGLWRLQEVLGHYRWPETLETDFI 949
GP F D+ + P V A+CSL+A IQR +GLWRLQEVL Y+WPE ++DFI
Sbjct: 791 -------GPCFPDIMKMMLPQHVIAVCSLVASIQRCTGLWRLQEVLDQYKWPEVEQSDFI 843
Query: 950 FGSSSIGS-VNAQFLAAFSAAVGKRSMQLSESEESDPDWGCWSISQESRNPSIKIIYPTI 1126
+GSSSIGS +NAQFL+AFSAA GKRS+QL +SEESDP+WGCW+ SQE RNPSI+II+PTI
Sbjct: 844 YGSSSIGSSINAQFLSAFSAAAGKRSLQLFDSEESDPEWGCWTKSQELRNPSIRIIFPTI 903
Query: 1127 ERVKHASCGILASKYLLCFSEKTWLRLRNVGILHDAIPVPIERVGHPMHVK--------- 1279
ERVK+A GIL+S+ +LCFSE TW RLR+ ILHDA+P P +RVGHPMHVK
Sbjct: 904 ERVKNACNGILSSRRILCFSENTWQRLRSAEILHDAVPHPYDRVGHPMHVKWPLNILYTR 963
Query: 1280 ---------VGLRRFQSKTSASSCGWVYCGSHNFSAAAWGRPLRYK---RPGGVGRINDV 1423
V R FQSKT SS GWVYCGSHNFSAAAWGRP+ + + G+ N
Sbjct: 964 ETDRENCKQVARRCFQSKTDVSSSGWVYCGSHNFSAAAWGRPICHPFGLKSNEPGKTNLS 1023
Query: 1424 LGSRLHISNYEVGVVFIVPPPDT 1492
G RLH+ NYE+G++F+ PP T
Sbjct: 1024 SGLRLHVCNYELGIIFVFPPSRT 1046
Score = 96.3 bits (238), Expect(2) = e-158
Identities = 44/57 (77%), Positives = 51/57 (89%)
Frame = +3
Query: 60 WLFVTNTVWWQDFPRLSTPDNSSLFTQSTDGEVNQDARSDFAAQLAKFIASLVVDVP 230
W VTNT+WWQDFPR STPD SSLFT+ +DGE++QD+RSDFAAQLA FIASLV+DVP
Sbjct: 566 WHNVTNTIWWQDFPRRSTPDLSSLFTRVSDGEISQDSRSDFAAQLAGFIASLVIDVP 622
>ref|XP_003630434.1| Tyrosyl-DNA phosphodiesterase [Medicago truncatula]
gi|355524456|gb|AET04910.1| Tyrosyl-DNA phosphodiesterase
[Medicago truncatula]
Length = 1064
Score = 461 bits (1187), Expect(2) = e-143
Identities = 234/425 (55%), Positives = 301/425 (70%), Gaps = 5/425 (1%)
Frame = +2
Query: 230 NQAHWIMELTKYDFRGAVAYLVTSVPGVYSPKIPYISDSRHYLNGPSYVSGLCGMKLLGS 409
+QAHWI +LTKYDF A +LV S+PG++ + + +S LGS
Sbjct: 555 SQAHWITQLTKYDFGSATGHLVASLPGIHLNRTSVLLESFQ------------SSSFLGS 602
Query: 410 IEASVVGLSHLFRASEDSSGSRIKKLAVFLRKCHENAYGMSEIVLKRNMNILADENAIAV 589
+ ASVVGLSHLFRA DS+ + ++ LA L K +N G EIVL+RN N+ ADENA++V
Sbjct: 603 VVASVVGLSHLFRAVADSNSAGLRALAAVLGKYCKNVNGRFEIVLRRNHNVPADENAVSV 662
Query: 590 LIPKPDKFDEGDCIQLGFLPRNIAKWVAPLSDAGLFSFSAYIYRKDVLTSALEGSNKKLT 769
L+PK D+ EGD +QLGFLPRN+AKWV+PL DAG FSFS Y+ K+ L +AL +++KL
Sbjct: 663 LVPKSDQTSEGDFVQLGFLPRNLAKWVSPLWDAGFFSFSGYVCPKEALAAALGENSQKLQ 722
Query: 770 LILYVSQGPSFADMSGVTQPSLVSAICSLIAVIQRSSGLWRLQEVLGHYRWPETLETDFI 949
LIL VS+G F DMS + Q ++A CSLIA IQR GLWRLQEVL YRWPE+LE++ +
Sbjct: 723 LILNVSEGHHFRDMSKMMQSEQIAAFCSLIASIQRHYGLWRLQEVLNQYRWPESLESEIV 782
Query: 950 FGSSSIGSVNAQFLAAFSAAVGKRSMQLSESEESDPDWGCWSISQESRNPSIKIIYPTIE 1129
+G+SSIGSVN++FLAAFSAA GK+S+Q +SEESDP+WGCW+ +E +NPS+KII+PTIE
Sbjct: 783 YGASSIGSVNSKFLAAFSAAAGKKSLQHFDSEESDPEWGCWNAREELKNPSVKIIFPTIE 842
Query: 1130 RVKHASCGILASKYLLCFSEKTWLRLRNVGILHDAIPVPIERVGHPMHVKVGLRRFQSKT 1309
RVK A GIL S+ +LCFSE+TW RL+ + +LHDA+P P ERVGHPMH KV R F S+
Sbjct: 843 RVKSAYNGILPSRRILCFSERTWQRLKTLDVLHDAVPHPHERVGHPMHTKVVRRCFWSRG 902
Query: 1310 SASSCGWVYCGSHNFSAAAWGRPLRYKRPGGV-----GRINDVLGSRLHISNYEVGVVFI 1474
A S GWVYCGSHNFSAAAWGR + P G + + + S LHI NYE+G++F
Sbjct: 903 EAPSIGWVYCGSHNFSAAAWGRQI--SNPFGTKADDPHKGDPSVNSGLHICNYELGIIFT 960
Query: 1475 VPPPD 1489
PP +
Sbjct: 961 FPPSE 965
Score = 75.1 bits (183), Expect(2) = e-143
Identities = 34/57 (59%), Positives = 44/57 (77%)
Frame = +3
Query: 60 WLFVTNTVWWQDFPRLSTPDNSSLFTQSTDGEVNQDARSDFAAQLAKFIASLVVDVP 230
W VTNT+WWQDFPR D +SLF + D EV+++++ DFAAQLA F+ASLV+DVP
Sbjct: 498 WNSVTNTIWWQDFPRAILVDYASLFRKIDDDEVHRNSKCDFAAQLAGFMASLVIDVP 554