BLASTX nr result
ID: Bupleurum21_contig00020702
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00020702
(1037 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002322991.1| predicted protein [Populus trichocarpa] gi|2... 503 e-140
ref|XP_002263020.1| PREDICTED: succinate/fumarate mitochondrial ... 497 e-138
ref|XP_004137702.1| PREDICTED: succinate/fumarate mitochondrial ... 493 e-137
ref|XP_003519192.1| PREDICTED: succinate/fumarate mitochondrial ... 493 e-137
gb|AFK42914.1| unknown [Lotus japonicus] 485 e-135
>ref|XP_002322991.1| predicted protein [Populus trichocarpa] gi|222867621|gb|EEF04752.1|
predicted protein [Populus trichocarpa]
Length = 310
Score = 503 bits (1295), Expect = e-140
Identities = 246/287 (85%), Positives = 264/287 (91%)
Frame = +1
Query: 175 NRDPPNQKKITVIPPYMKAISGSIGGIVEASCLQPIDVIKTRLQLDRSGSYKGILHCGGT 354
+++ N KK T IPPYMKAISGS+GG VEASCLQPIDVIKTRLQLDRSG+YKGI+HCG T
Sbjct: 2 SQESSNDKKPT-IPPYMKAISGSLGGAVEASCLQPIDVIKTRLQLDRSGNYKGIIHCGST 60
Query: 355 VVKTEGVRALWKGLTPFATHLTLKYALRMGSNAVLQTAFKDNQTGHISNPGRILSGFGAG 534
+VKTEGVRALWKGLTPFATHLTLKYALRMGSNAV Q+AFKD++TG +SN GR++SGFGAG
Sbjct: 61 IVKTEGVRALWKGLTPFATHLTLKYALRMGSNAVFQSAFKDSETGKLSNQGRLMSGFGAG 120
Query: 535 VLEALVIVTPFEVVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLRGLWAGAAPTVMR 714
VLEAL IVTPFEVVKIRLQQQKGLSPELLKYKGPIHCA II E+G+ GLWAGAAPTVMR
Sbjct: 121 VLEALAIVTPFEVVKIRLQQQKGLSPELLKYKGPIHCAHTIIREEGVLGLWAGAAPTVMR 180
Query: 715 NGTNQAAMFTAKNAFDKFLWKKHEGDGRVLLPWQSMISGFLAGTAGPVCTGPFDVVKTRL 894
NGTNQAAMFTAKNAFD LWKKHEGDGRVL PWQSMISGFLAGTAGPVCTGPFDVVKTRL
Sbjct: 181 NGTNQAAMFTAKNAFDVLLWKKHEGDGRVLQPWQSMISGFLAGTAGPVCTGPFDVVKTRL 240
Query: 895 MAQSRSGGDMRYTGMFHAISTIYAEEGLRALWKGLLPRLMRIPPGQA 1035
MAQSR GG+++Y GM HAI TIYAEEGL ALWKGLLPRLMRIPPGQA
Sbjct: 241 MAQSREGGELKYKGMVHAIRTIYAEEGLLALWKGLLPRLMRIPPGQA 287
Score = 93.2 bits (230), Expect = 9e-17
Identities = 67/181 (37%), Positives = 94/181 (51%), Gaps = 9/181 (4%)
Frame = +1
Query: 226 KAISGSIGGIVEA-SCLQPIDVIKTRLQLDRSGS-----YKGILHCGGTVVKTEGVRALW 387
+ +SG G++EA + + P +V+K RLQ + S YKG +HC T+++ EGV LW
Sbjct: 112 RLMSGFGAGVLEALAIVTPFEVVKIRLQQQKGLSPELLKYKGPIHCAHTIIREEGVLGLW 171
Query: 388 KGLTPFATHL-TLKYALRMGSNAVLQTAFKDNQ-TGHISNPGR-ILSGFGAGVLEALVIV 558
G P T + A+ NA +K ++ G + P + ++SGF AG V
Sbjct: 172 AGAAPTVMRNGTNQAAMFTAKNAFDVLLWKKHEGDGRVLQPWQSMISGFLAGTAGP-VCT 230
Query: 559 TPFEVVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLRGLWAGAAPTVMRNGTNQAAM 738
PF+VVK RL Q EL KYKG +H R I E+GL LW G P +MR QA M
Sbjct: 231 GPFDVVKTRLMAQSREGGEL-KYKGMVHAIRTIYAEEGLLALWKGLLPRLMRIPPGQAIM 289
Query: 739 F 741
+
Sbjct: 290 W 290
>ref|XP_002263020.1| PREDICTED: succinate/fumarate mitochondrial transporter [Vitis
vinifera] gi|147857058|emb|CAN81800.1| hypothetical
protein VITISV_020062 [Vitis vinifera]
gi|296081115|emb|CBI18247.3| unnamed protein product
[Vitis vinifera]
Length = 306
Score = 497 bits (1279), Expect = e-138
Identities = 241/282 (85%), Positives = 260/282 (92%)
Frame = +1
Query: 190 NQKKITVIPPYMKAISGSIGGIVEASCLQPIDVIKTRLQLDRSGSYKGILHCGGTVVKTE 369
++ K T IPPY+KA+SGSIGGIVEASCLQPIDVIKTRLQLD SG+YKGI+HCG TV +TE
Sbjct: 2 SESKKTTIPPYVKALSGSIGGIVEASCLQPIDVIKTRLQLDTSGTYKGIIHCGATVYRTE 61
Query: 370 GVRALWKGLTPFATHLTLKYALRMGSNAVLQTAFKDNQTGHISNPGRILSGFGAGVLEAL 549
GVRALWKGLTPFATHLTLKY LRMGSNA+ Q+AFKD+QTG +SN GR+LSGFGAGVLEAL
Sbjct: 62 GVRALWKGLTPFATHLTLKYTLRMGSNALFQSAFKDSQTGQLSNTGRLLSGFGAGVLEAL 121
Query: 550 VIVTPFEVVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLRGLWAGAAPTVMRNGTNQ 729
VIVTPFEVVKIRLQQQ+GLSPELLKYKGPIHCAR II E+GLRGLWAGAAPTVMRNGTNQ
Sbjct: 122 VIVTPFEVVKIRLQQQRGLSPELLKYKGPIHCARTIIREEGLRGLWAGAAPTVMRNGTNQ 181
Query: 730 AAMFTAKNAFDKFLWKKHEGDGRVLLPWQSMISGFLAGTAGPVCTGPFDVVKTRLMAQSR 909
AAMFTAKNAFD LWKK EGDG+VL PWQSMISGFLAGTAGPVCTGPFDVVKTRLMAQ R
Sbjct: 182 AAMFTAKNAFDGILWKKQEGDGKVLQPWQSMISGFLAGTAGPVCTGPFDVVKTRLMAQGR 241
Query: 910 SGGDMRYTGMFHAISTIYAEEGLRALWKGLLPRLMRIPPGQA 1035
+GG ++Y GM HAI TI+AEEGL ALWKGLLPRLMRIPPGQA
Sbjct: 242 AGGKLKYKGMVHAIRTIFAEEGLLALWKGLLPRLMRIPPGQA 283
Score = 98.2 bits (243), Expect = 3e-18
Identities = 70/195 (35%), Positives = 99/195 (50%), Gaps = 9/195 (4%)
Frame = +1
Query: 226 KAISGSIGGIVEASCL-QPIDVIKTRLQLDRSGS-----YKGILHCGGTVVKTEGVRALW 387
+ +SG G++EA + P +V+K RLQ R S YKG +HC T+++ EG+R LW
Sbjct: 108 RLLSGFGAGVLEALVIVTPFEVVKIRLQQQRGLSPELLKYKGPIHCARTIIREEGLRGLW 167
Query: 388 KGLTPFATHL-TLKYALRMGSNAVLQTAFKDNQ-TGHISNPGR-ILSGFGAGVLEALVIV 558
G P T + A+ NA +K + G + P + ++SGF AG V
Sbjct: 168 AGAAPTVMRNGTNQAAMFTAKNAFDGILWKKQEGDGKVLQPWQSMISGFLAGTAGP-VCT 226
Query: 559 TPFEVVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLRGLWAGAAPTVMRNGTNQAAM 738
PF+VVK RL Q G + LKYKG +H R I E+GL LW G P +MR QA M
Sbjct: 227 GPFDVVKTRLMAQ-GRAGGKLKYKGMVHAIRTIFAEEGLLALWKGLLPRLMRIPPGQAIM 285
Query: 739 FTAKNAFDKFLWKKH 783
+ + F ++H
Sbjct: 286 WGVADQVTGFYERRH 300
>ref|XP_004137702.1| PREDICTED: succinate/fumarate mitochondrial transporter-like [Cucumis
sativus] gi|449483528|ref|XP_004156616.1| PREDICTED:
succinate/fumarate mitochondrial transporter-like
[Cucumis sativus]
Length = 317
Score = 493 bits (1269), Expect = e-137
Identities = 240/288 (83%), Positives = 265/288 (92%)
Frame = +1
Query: 172 ANRDPPNQKKITVIPPYMKAISGSIGGIVEASCLQPIDVIKTRLQLDRSGSYKGILHCGG 351
+N++ + KK IPPYMKAISGS+GG++EA CLQPIDVIKTRLQLDRSG+YKGI+HCG
Sbjct: 9 SNKEQSSTKKS--IPPYMKAISGSLGGVMEACCLQPIDVIKTRLQLDRSGAYKGIVHCGT 66
Query: 352 TVVKTEGVRALWKGLTPFATHLTLKYALRMGSNAVLQTAFKDNQTGHISNPGRILSGFGA 531
TV +TEGVRALWKGLTPFATHLTLKYALRMGSNAVLQTAFKD++TG +SN R++SGFGA
Sbjct: 67 TVTQTEGVRALWKGLTPFATHLTLKYALRMGSNAVLQTAFKDSETGKLSNHARLISGFGA 126
Query: 532 GVLEALVIVTPFEVVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLRGLWAGAAPTVM 711
GVLEALVIVTPFEVVKIRLQQQKGL+PELLKYKGP+HCAR+II E+GL GLWAGAAPTVM
Sbjct: 127 GVLEALVIVTPFEVVKIRLQQQKGLTPELLKYKGPVHCARMIIREEGLLGLWAGAAPTVM 186
Query: 712 RNGTNQAAMFTAKNAFDKFLWKKHEGDGRVLLPWQSMISGFLAGTAGPVCTGPFDVVKTR 891
RNGTNQAAMFTAKNAFD LW +HEGDG+VL PWQSMISGFLAGTAGP+CTGPFDVVKTR
Sbjct: 187 RNGTNQAAMFTAKNAFDIVLWNRHEGDGQVLQPWQSMISGFLAGTAGPLCTGPFDVVKTR 246
Query: 892 LMAQSRSGGDMRYTGMFHAISTIYAEEGLRALWKGLLPRLMRIPPGQA 1035
LMAQSR G+++Y GMFHAI TIYAEEGL ALWKGLLPRLMRIPPGQA
Sbjct: 247 LMAQSRGTGELKYKGMFHAIRTIYAEEGLFALWKGLLPRLMRIPPGQA 294
Score = 84.3 bits (207), Expect = 4e-14
Identities = 62/185 (33%), Positives = 92/185 (49%), Gaps = 11/185 (5%)
Frame = +1
Query: 220 YMKAISGSIGGIVEASCL-QPIDVIKTRLQLDRSGS-----YKGILHCGGTVVKTEGVRA 381
+ + ISG G++EA + P +V+K RLQ + + YKG +HC +++ EG+
Sbjct: 117 HARLISGFGAGVLEALVIVTPFEVVKIRLQQQKGLTPELLKYKGPVHCARMIIREEGLLG 176
Query: 382 LWKGLTPFA----THLTLKYALRMGSNAVLQTAFKDNQTGHISNPGR-ILSGFGAGVLEA 546
LW G P T+ + + + VL + + G + P + ++SGF AG
Sbjct: 177 LWAGAAPTVMRNGTNQAAMFTAKNAFDIVLWNRHEGD--GQVLQPWQSMISGFLAGTAGP 234
Query: 547 LVIVTPFEVVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLRGLWAGAAPTVMRNGTN 726
L PF+VVK RL Q + EL KYKG H R I E+GL LW G P +MR
Sbjct: 235 LC-TGPFDVVKTRLMAQSRGTGEL-KYKGMFHAIRTIYAEEGLFALWKGLLPRLMRIPPG 292
Query: 727 QAAMF 741
QA ++
Sbjct: 293 QAIVW 297
>ref|XP_003519192.1| PREDICTED: succinate/fumarate mitochondrial transporter-like [Glycine
max]
Length = 334
Score = 493 bits (1269), Expect = e-137
Identities = 240/287 (83%), Positives = 260/287 (90%), Gaps = 1/287 (0%)
Frame = +1
Query: 178 RDPPNQKKITVIPPYMKAISGSIGGIVEASCLQPIDVIKTRLQLDRSGSYKGILHCGGTV 357
+D N IPPYMKAISGS+GGI+EASCLQPIDVIKTRLQLDRSG+YKGILHCG T+
Sbjct: 25 QDNTNSYSKNSIPPYMKAISGSLGGIMEASCLQPIDVIKTRLQLDRSGNYKGILHCGATI 84
Query: 358 VKTEGVRALWKGLTPFATHLTLKYALRMGSNAVLQTAFKDNQTGHISNPGRILSGFGAGV 537
+TEGVRALWKGLTPFATHLTLKYALRMGSNAVLQ+AFKD +TG +S GRILSGFGAGV
Sbjct: 85 SRTEGVRALWKGLTPFATHLTLKYALRMGSNAVLQSAFKDPETGKLSGYGRILSGFGAGV 144
Query: 538 LEALVIVTPFEVVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLRGLWAGAAPTVMRN 717
LEA++IVTPFEVVKIRLQQQ+GLSPELLKYKGP+HCAR+II E+G RGLWAG APTVMRN
Sbjct: 145 LEAIIIVTPFEVVKIRLQQQRGLSPELLKYKGPVHCARMIIREEGFRGLWAGVAPTVMRN 204
Query: 718 GTNQAAMFTAKNAFDKFLWKKHEGDGRVLLPWQSMISGFLAGTAGPVCTGPFDVVKTRLM 897
GTNQ+AMFTAKNAFD LWKKHEGDGRVLLPWQSMISGFLAGTAGP+CTGPFDVVKTRLM
Sbjct: 205 GTNQSAMFTAKNAFDVLLWKKHEGDGRVLLPWQSMISGFLAGTAGPICTGPFDVVKTRLM 264
Query: 898 AQSRSGGD-MRYTGMFHAISTIYAEEGLRALWKGLLPRLMRIPPGQA 1035
AQ+R GG ++Y GM HAI TIY EEGL ALWKGLLPRLMRIPPGQA
Sbjct: 265 AQTREGGGVLKYKGMIHAIRTIYVEEGLLALWKGLLPRLMRIPPGQA 311
Score = 96.7 bits (239), Expect = 8e-18
Identities = 71/201 (35%), Positives = 102/201 (50%), Gaps = 9/201 (4%)
Frame = +1
Query: 166 EAANRDPPNQKKITVIPPYMKAISGSIGGIVEASCL-QPIDVIKTRLQLDRSGS-----Y 327
++A +DP K + Y + +SG G++EA + P +V+K RLQ R S Y
Sbjct: 119 QSAFKDPETGK----LSGYGRILSGFGAGVLEAIIIVTPFEVVKIRLQQQRGLSPELLKY 174
Query: 328 KGILHCGGTVVKTEGVRALWKGLTPFATHL-TLKYALRMGSNAVLQTAFKDNQ-TGHISN 501
KG +HC +++ EG R LW G+ P T + A+ NA +K ++ G +
Sbjct: 175 KGPVHCARMIIREEGFRGLWAGVAPTVMRNGTNQSAMFTAKNAFDVLLWKKHEGDGRVLL 234
Query: 502 PGR-ILSGFGAGVLEALVIVTPFEVVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLR 678
P + ++SGF AG + PF+VVK RL Q +LKYKG IH R I E+GL
Sbjct: 235 PWQSMISGFLAGTAGP-ICTGPFDVVKTRLMAQTREGGGVLKYKGMIHAIRTIYVEEGLL 293
Query: 679 GLWAGAAPTVMRNGTNQAAMF 741
LW G P +MR QA M+
Sbjct: 294 ALWKGLLPRLMRIPPGQAIMW 314
>gb|AFK42914.1| unknown [Lotus japonicus]
Length = 313
Score = 485 bits (1248), Expect = e-135
Identities = 227/275 (82%), Positives = 255/275 (92%)
Frame = +1
Query: 211 IPPYMKAISGSIGGIVEASCLQPIDVIKTRLQLDRSGSYKGILHCGGTVVKTEGVRALWK 390
IPPY+KAISGS+GG+VEASCLQPIDVIKTRLQLDRSG+YKGI+HCG T+ +TEGVRALWK
Sbjct: 16 IPPYLKAISGSLGGVVEASCLQPIDVIKTRLQLDRSGNYKGIVHCGSTISRTEGVRALWK 75
Query: 391 GLTPFATHLTLKYALRMGSNAVLQTAFKDNQTGHISNPGRILSGFGAGVLEALVIVTPFE 570
GLTPFATHLT KYALRMGSNAV Q+ FKD++TG +S+ GR+LSGFGAGVLEA+VIVTPFE
Sbjct: 76 GLTPFATHLTFKYALRMGSNAVFQSMFKDSETGKLSSHGRLLSGFGAGVLEAIVIVTPFE 135
Query: 571 VVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLRGLWAGAAPTVMRNGTNQAAMFTAK 750
VVKI+LQQQ+GLSPELLKYKGP+HCAR I+HE+ +RGLWAG +PT+MRNGTNQ+ MF+AK
Sbjct: 136 VVKIKLQQQRGLSPELLKYKGPVHCARTILHEESIRGLWAGVSPTIMRNGTNQSVMFSAK 195
Query: 751 NAFDKFLWKKHEGDGRVLLPWQSMISGFLAGTAGPVCTGPFDVVKTRLMAQSRSGGDMRY 930
NAFD LWKKHEGDG VL PWQSMISGFLAGTAGP CTGPFDVVKTRLMAQSR GG+++Y
Sbjct: 196 NAFDVLLWKKHEGDGSVLQPWQSMISGFLAGTAGPFCTGPFDVVKTRLMAQSREGGELKY 255
Query: 931 TGMFHAISTIYAEEGLRALWKGLLPRLMRIPPGQA 1035
GM HAI TIY+EEGLRALWKGLLPRLMRIPPGQA
Sbjct: 256 KGMIHAIRTIYSEEGLRALWKGLLPRLMRIPPGQA 290
Score = 92.8 bits (229), Expect = 1e-16
Identities = 65/183 (35%), Positives = 95/183 (51%), Gaps = 11/183 (6%)
Frame = +1
Query: 226 KAISGSIGGIVEASCL-QPIDVIKTRLQLDRSGS-----YKGILHCGGTVVKTEGVRALW 387
+ +SG G++EA + P +V+K +LQ R S YKG +HC T++ E +R LW
Sbjct: 115 RLLSGFGAGVLEAIVIVTPFEVVKIKLQQQRGLSPELLKYKGPVHCARTILHEESIRGLW 174
Query: 388 KGLTPF----ATHLTLKYALRMGSNAVLQTAFKDNQTGHISNPGR-ILSGFGAGVLEALV 552
G++P T+ ++ ++ + + +L K G + P + ++SGF AG
Sbjct: 175 AGVSPTIMRNGTNQSVMFSAKNAFDVLLWK--KHEGDGSVLQPWQSMISGFLAGTAGPFC 232
Query: 553 IVTPFEVVKIRLQQQKGLSPELLKYKGPIHCARIIIHEDGLRGLWAGAAPTVMRNGTNQA 732
PF+VVK RL Q EL KYKG IH R I E+GLR LW G P +MR QA
Sbjct: 233 -TGPFDVVKTRLMAQSREGGEL-KYKGMIHAIRTIYSEEGLRALWKGLLPRLMRIPPGQA 290
Query: 733 AMF 741
M+
Sbjct: 291 IMW 293
Score = 59.7 bits (143), Expect = 1e-06
Identities = 31/70 (44%), Positives = 40/70 (57%), Gaps = 4/70 (5%)
Frame = +1
Query: 205 TVIPPYMKAISGSIGGIVEASCLQPIDVIKTRLQL-DRSG---SYKGILHCGGTVVKTEG 372
+V+ P+ ISG + G C P DV+KTRL R G YKG++H T+ EG
Sbjct: 211 SVLQPWQSMISGFLAGTAGPFCTGPFDVVKTRLMAQSREGGELKYKGMIHAIRTIYSEEG 270
Query: 373 VRALWKGLTP 402
+RALWKGL P
Sbjct: 271 LRALWKGLLP 280