BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020693
         (1650 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284970.2| PREDICTED: uncharacterized protein LOC100267...   260   6e-82
emb|CAN81424.1| hypothetical protein VITISV_035944 [Vitis vinifera]   259   7e-82
ref|XP_002521841.1| conserved hypothetical protein [Ricinus comm...   231   9e-74
ref|XP_002330275.1| predicted protein [Populus trichocarpa] gi|2...   208   1e-65
ref|XP_004145354.1| PREDICTED: uncharacterized protein LOC101214...   215   2e-63

>ref|XP_002284970.2| PREDICTED: uncharacterized protein LOC100267475 [Vitis vinifera]
           gi|297741334|emb|CBI32465.3| unnamed protein product
           [Vitis vinifera]
          Length = 401

 Score =  260 bits (664), Expect(2) = 6e-82
 Identities = 149/296 (50%), Positives = 192/296 (64%), Gaps = 4/296 (1%)
 Frame = +2

Query: 119 PQELSLNIKPTFIPKTISEFLGEVSSIRNVSEKLLKLDNFIKRLEDEMRKIDAFKRELPL 298
           P ELSL+++P+++PKTI++FL  +S+I +VSE++ KLD F+KRLE+EMRKIDAFKRELPL
Sbjct: 5   PPELSLDLRPSYMPKTINDFLSGISTIGDVSERVTKLDEFLKRLEEEMRKIDAFKRELPL 64

Query: 299 SVILISDAIVALKEELSQCRKTQVEPVFEEFIPLKKIGDESENDEKARIEKESKDKMNWM 478
            +IL+SDAI+ALKEEL + + + V+PV EEFIPLKK  DE   +  A+ EKE KDK NWM
Sbjct: 65  CMILLSDAILALKEELLRSKASNVQPVLEEFIPLKKDCDE---EGGAKEEKECKDKKNWM 121

Query: 479 SSVQLWSTTGDQNTRNTDSNCSREPNSIVEIAKKSEQGTDVKMEDVYQSYKGRGEASAFV 658
           SSVQLW++    ++ +TD    ++ +S +EI ++ ++      ED +Q  K R    AF+
Sbjct: 122 SSVQLWNS---DDSASTDHIYDKKQDSKLEIKQRVDEENRPATEDPFQPCKSRTGGRAFL 178

Query: 659 PFKGYSASPVAAMRKDAKVEDSEEFPAAGLSLLTPGTCKARVELGXXXXXXXXXXXXXXX 838
           PFKGYS  PVA  RK    ED +E P  GLSLLTPG    R E G               
Sbjct: 179 PFKGYSGFPVATARK----EDKDELPVHGLSLLTPGIKNPREESG--SSGSKTSCSRGVS 232

Query: 839 XXGPNVQSNL----XXXXXXXXXXXXXCWSPELHRRFVSALQQLGGAQVATPKQIR 994
              PN+Q NL                 CWSPELHRRFV+ALQQLGG+Q ATPKQIR
Sbjct: 233 SSAPNLQPNLRTGPQPPQQQTARKQRRCWSPELHRRFVNALQQLGGSQAATPKQIR 288



 Score = 72.8 bits (177), Expect(2) = 6e-82
 Identities = 41/87 (47%), Positives = 55/87 (63%), Gaps = 5/87 (5%)
 Frame = +1

Query: 1072 KYRLHNRRSPTNKSADST----LTLGSVLKISGD-GESSSMNRSKSTSPEGPPQLTDALV 1236
            KYRLH RR PT  +A  +    + LGS+       G+SS  + S+S SP+GP QL  A  
Sbjct: 307  KYRLHTRRMPTTSAAPPSNQPVVVLGSLWMPQDQYGDSSKASSSQSASPQGPLQLGGAAG 366

Query: 1237 KTSTTFGDTMGDKQDERSKSYSWKNRV 1317
             TSTT GD+M D +DE+S+SY WK++V
Sbjct: 367  GTSTTGGDSMEDDEDEKSESYGWKSQV 393


>emb|CAN81424.1| hypothetical protein VITISV_035944 [Vitis vinifera]
          Length = 401

 Score =  259 bits (663), Expect(2) = 7e-82
 Identities = 148/296 (50%), Positives = 192/296 (64%), Gaps = 4/296 (1%)
 Frame = +2

Query: 119 PQELSLNIKPTFIPKTISEFLGEVSSIRNVSEKLLKLDNFIKRLEDEMRKIDAFKRELPL 298
           P ELSL+++P+++PKTI++FL  +S+I +VSE++ KLD F+KRLE+EMRKIDAFKRELPL
Sbjct: 5   PPELSLDLRPSYVPKTINDFLSGISTIGDVSERVTKLDEFLKRLEEEMRKIDAFKRELPL 64

Query: 299 SVILISDAIVALKEELSQCRKTQVEPVFEEFIPLKKIGDESENDEKARIEKESKDKMNWM 478
            +IL+SDAI+ALKEEL + + + V+PV EEFIPLKK  DE   +  A+ EK+ KDK NWM
Sbjct: 65  CMILLSDAILALKEELLRSKASNVQPVLEEFIPLKKDCDE---EGGAKEEKDCKDKKNWM 121

Query: 479 SSVQLWSTTGDQNTRNTDSNCSREPNSIVEIAKKSEQGTDVKMEDVYQSYKGRGEASAFV 658
           SSVQLW++    ++ +TD    ++ +S +EI ++ ++      ED +Q  K R    AF+
Sbjct: 122 SSVQLWNS---DDSASTDHIYDKKQDSKLEIKQRVDEENRPATEDPFQPCKSRTGGRAFL 178

Query: 659 PFKGYSASPVAAMRKDAKVEDSEEFPAAGLSLLTPGTCKARVELGXXXXXXXXXXXXXXX 838
           PFKGYS  PVA  RK    ED +E P  GLSLLTPG    R E G               
Sbjct: 179 PFKGYSGFPVATARK----EDKDELPVHGLSLLTPGIKNPREESG--SSGSKTSCSRGVS 232

Query: 839 XXGPNVQSNL----XXXXXXXXXXXXXCWSPELHRRFVSALQQLGGAQVATPKQIR 994
              PN+Q NL                 CWSPELHRRFV+ALQQLGG+Q ATPKQIR
Sbjct: 233 SSAPNLQPNLRTGPQPPQQQTARKQRRCWSPELHRRFVNALQQLGGSQAATPKQIR 288



 Score = 72.8 bits (177), Expect(2) = 7e-82
 Identities = 41/87 (47%), Positives = 55/87 (63%), Gaps = 5/87 (5%)
 Frame = +1

Query: 1072 KYRLHNRRSPTNKSADST----LTLGSVLKISGD-GESSSMNRSKSTSPEGPPQLTDALV 1236
            KYRLH RR PT  +A  +    + LGS+       G+SS  + S+S SP+GP QL  A  
Sbjct: 307  KYRLHTRRMPTTSAAPPSNQPVVVLGSLWMPQDQYGDSSKASSSQSASPQGPLQLGGAAG 366

Query: 1237 KTSTTFGDTMGDKQDERSKSYSWKNRV 1317
             TSTT GD+M D +DE+S+SY WK++V
Sbjct: 367  GTSTTGGDSMEDDEDEKSESYGWKSQV 393


>ref|XP_002521841.1| conserved hypothetical protein [Ricinus communis]
           gi|223538879|gb|EEF40477.1| conserved hypothetical
           protein [Ricinus communis]
          Length = 408

 Score =  231 bits (588), Expect(2) = 9e-74
 Identities = 135/301 (44%), Positives = 176/301 (58%), Gaps = 9/301 (2%)
 Frame = +2

Query: 119 PQELSLNIKPTFIPKTISEFLGEVSSIRNVSEKLLKLDNFIKRLEDEMRKIDAFKRELPL 298
           P ELSL+ +P+++PKTIS+ L EVS IRNVS+K+ KLD F+  L++EM+KIDAFKRELPL
Sbjct: 5   PPELSLDFRPSYVPKTISDILNEVSLIRNVSDKVSKLDGFVNGLQEEMKKIDAFKRELPL 64

Query: 299 SVILISDAIVALKEELSQCRKTQVEPVFEEFIPLKKIGDESENDE-----KARIEKESKD 463
            ++L++DAI+ LK EL QC     +P+ EEF+PLKK  D  ++D+       + EK++KD
Sbjct: 65  CMLLLNDAILFLKSELMQCAALDHQPILEEFMPLKKHSDRDDDDDGDQDGPIKKEKDTKD 124

Query: 464 KMNWMSSVQLWSTTGDQNTRNTDSNCSREPNSIVEIAKKSEQGTDVKMEDVYQSYKGRGE 643
           K NWMSSVQLW+T    +T N       + N  +E     +       +D + + K R  
Sbjct: 125 KKNWMSSVQLWNTDDHPSTHNI---LETKQNLQLESKTTKKGNYQYANDDTFSACKSRSA 181

Query: 644 ASAFVPFKGYSASPVAAMRKDAKVEDSEEFPAAGLSLLTPGTCKARVELGXXXXXXXXXX 823
           A AF+PFK Y   P  + ++D     ++E P  GLSLLTPG    R E            
Sbjct: 182 ARAFMPFKTY---PDLSRKEDK--HSTQELPVPGLSLLTPGIKNLREE--STSTGSRVSC 234

Query: 824 XXXXXXXGPNVQSNL----XXXXXXXXXXXXXCWSPELHRRFVSALQQLGGAQVATPKQI 991
                   PN QSNL                 CWSPELHRRFVSALQQLGG+Q ATPKQI
Sbjct: 235 SRAVSSSTPNPQSNLRNGPQSPQQQTARKQRRCWSPELHRRFVSALQQLGGSQAATPKQI 294

Query: 992 R 994
           R
Sbjct: 295 R 295



 Score = 74.7 bits (182), Expect(2) = 9e-74
 Identities = 43/95 (45%), Positives = 58/95 (61%), Gaps = 5/95 (5%)
 Frame = +1

Query: 1072 KYRLHNRRSPTNKSA---DSTLTLGSVLKISGD--GESSSMNRSKSTSPEGPPQLTDALV 1236
            KYRLH RR P+  +A    S + LG  L++S D  G+ S    S+S SP+GP QL     
Sbjct: 314  KYRLHTRRMPSATAAATNHSLVVLGGGLRMSQDQYGDDSKAAGSQSGSPQGPLQLAGNTG 373

Query: 1237 KTSTTFGDTMGDKQDERSKSYSWKNRVEAPGKVAV 1341
             TSTT GD+M D +D +S+ YSWK+++   GKV V
Sbjct: 374  ATSTTGGDSMEDDEDAKSEGYSWKSQIHRSGKVHV 408


>ref|XP_002330275.1| predicted protein [Populus trichocarpa] gi|222871310|gb|EEF08441.1|
           predicted protein [Populus trichocarpa]
          Length = 420

 Score =  208 bits (530), Expect(2) = 1e-65
 Identities = 134/292 (45%), Positives = 172/292 (58%), Gaps = 10/292 (3%)
 Frame = +2

Query: 149 TFIPKTISEFLGEVSSIRNVSEKLLKLDNFIKRLEDEMRKIDAFKRELPLSVILISDAIV 328
           TF+P+TI++FL EVS I + + K LK+D+F+  LE+E RKIDAFKRELPL ++L++DAI 
Sbjct: 30  TFLPETITDFLKEVSMIGDAAVKGLKVDSFLNDLEEEKRKIDAFKRELPLCMLLLNDAIQ 89

Query: 329 ALKEELSQC-RKTQVEPVFEEFIPLKK----IGDESENDEKARIEKESKDKMNWMSSVQL 493
            ++EEL QC      +PV EEFIPLKK     GD+ E+D   + EK+SKDK NWMSSVQL
Sbjct: 90  VVREELMQCGTSNNQQPVLEEFIPLKKKIDDHGDDRESDGLIK-EKDSKDKKNWMSSVQL 148

Query: 494 WSTTGDQNTRNTDSNCSREPNSIVEIAKKSEQGTDVKMEDVYQSYKGRGEASAFVPFKGY 673
           W T  D +  +TD     + +  +E +K +++      ED +Q+ KGR  A  F+PFK Y
Sbjct: 149 WIT--DDHHPSTDYLFDAKQSFKLE-SKTNKKANQYVNEDAFQACKGRTAARTFMPFKAY 205

Query: 674 SASPVAAMRKDAKVEDS--EEFPAAGLSLLTPGTCKARVELGXXXXXXXXXXXXXXXXXG 847
             S         K +DS  EE P   LSLLTPG    + E                    
Sbjct: 206 PGS-------SRKEDDSNREELPVPALSLLTPGIKSIKEE--SNSTGSRSSCSRSVSTTA 256

Query: 848 PNVQSNL---XXXXXXXXXXXXXCWSPELHRRFVSALQQLGGAQVATPKQIR 994
           PN +SNL                CWSPELHR+FV+ALQQLGGAQVATPKQIR
Sbjct: 257 PNSESNLRNGPQSQQQSSRKHRRCWSPELHRQFVNALQQLGGAQVATPKQIR 308



 Score = 70.1 bits (170), Expect(2) = 1e-65
 Identities = 40/92 (43%), Positives = 52/92 (56%), Gaps = 5/92 (5%)
 Frame = +1

Query: 1072 KYRLHNRRSPTNKSA----DSTLTLGSVLKISGD-GESSSMNRSKSTSPEGPPQLTDALV 1236
            KYRLH RR P   ++     S + LG +       G+SS  N SKS SP+GP QL     
Sbjct: 327  KYRLHTRRVPPATASAPANQSAIVLGGLWMAQDQYGDSSKANSSKSGSPQGPLQLAVNTG 386

Query: 1237 KTSTTFGDTMGDKQDERSKSYSWKNRVEAPGK 1332
             TSTT GD+M D +D +S+ YSWK+ +   GK
Sbjct: 387  GTSTTGGDSMEDDEDAKSEGYSWKSHIHRSGK 418


>ref|XP_004145354.1| PREDICTED: uncharacterized protein LOC101214647 [Cucumis sativus]
           gi|449472655|ref|XP_004153659.1| PREDICTED:
           uncharacterized protein LOC101222527 [Cucumis sativus]
           gi|449511920|ref|XP_004164089.1| PREDICTED:
           uncharacterized LOC101222527 [Cucumis sativus]
          Length = 389

 Score =  215 bits (548), Expect(2) = 2e-63
 Identities = 128/294 (43%), Positives = 173/294 (58%), Gaps = 2/294 (0%)
 Frame = +2

Query: 119 PQELSLNIKPTFIPKTISEFLGEVSSIRNVSEKLLKLDNFIKRLEDEMRKIDAFKRELPL 298
           P EL+L+ +P+F+PKTI++F  EVS I NVS+++ KL++FIK LEDE+RKIDAFKRELPL
Sbjct: 5   PSELTLDFRPSFVPKTITDFFKEVSMIGNVSDRVSKLNDFIKTLEDEVRKIDAFKRELPL 64

Query: 299 SVILISDAIVALKEELSQCRKTQVEPVFEEFIPLKKIGDESENDEKARIEKESKDKMNWM 478
            ++L+ DAI+A+K+E  QC   + +PV EEFIPLKK  +E + D+  +   + +D+ NWM
Sbjct: 65  CMVLLKDAILAVKDEKMQCAVPKTKPVLEEFIPLKKEQEEDDGDDSKK-GNDYRDQKNWM 123

Query: 479 SSVQLWSTTGDQNTRNTDSNCSREPNSIVEIAKKSEQGTDVKMEDVYQSYKGRGEASAFV 658
           SSVQLW++       N  SN   E        K++E+G  V  +   QS + +      V
Sbjct: 124 SSVQLWNSDD-----NHHSNYKLE-------TKRNEKGGPVVTQVSMQSCRTKNGERIQV 171

Query: 659 PFKGYSASPVAAMRKDAKVEDSEEFPAAGLSLLTPGTCKARVELG--XXXXXXXXXXXXX 832
           PFK   + P+ +    A+ ED EEFP  GLSL TPG      E                 
Sbjct: 172 PFK--PSYPIFSSAMVARKEDKEEFPIHGLSLCTPGIKSPMEESASTGSRSSGTRAVSSS 229

Query: 833 XXXXGPNVQSNLXXXXXXXXXXXXXCWSPELHRRFVSALQQLGGAQVATPKQIR 994
                 N+++ +             CWS ELHRRFVSALQQLGG+QVATPKQIR
Sbjct: 230 TLTASVNLRTGMQQQKQQCSRKQRRCWSKELHRRFVSALQQLGGSQVATPKQIR 283



 Score = 55.5 bits (132), Expect(2) = 2e-63
 Identities = 34/91 (37%), Positives = 51/91 (56%), Gaps = 5/91 (5%)
 Frame = +1

Query: 1072 KYRLHNRRSPTNK----SADSTLTLGSVLKISGD-GESSSMNRSKSTSPEGPPQLTDALV 1236
            K+RLH RR P +     +  S + LG +L       +SS    S+S SP+GP QL     
Sbjct: 302  KFRLHARRLPASAVPPANQSSVVVLGGLLVPQDPYADSSKACSSQSGSPQGPLQLAG--- 358

Query: 1237 KTSTTFGDTMGDKQDERSKSYSWKNRVEAPG 1329
                T GD+M +++D +S+SY WK+R++ PG
Sbjct: 359  ----TGGDSMEEEEDVKSESYCWKSRIQKPG 385