BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020573
         (1022 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002267492.1| PREDICTED: solute carrier family 35 member F...   374   e-101
ref|XP_003622936.1| Solute carrier family 35 member F5 [Medicago...   361   1e-97
ref|XP_002882510.1| membrane protein [Arabidopsis lyrata subsp. ...   360   2e-97
ref|NP_187364.1| EamA-like transporter [Arabidopsis thaliana] gi...   358   9e-97
ref|XP_002521105.1| conserved hypothetical protein [Ricinus comm...   355   8e-96

>ref|XP_002267492.1| PREDICTED: solute carrier family 35 member F5 [Vitis vinifera]
          Length = 435

 Score =  374 bits (961), Expect = e-101
 Identities = 206/318 (64%), Positives = 235/318 (73%), Gaps = 11/318 (3%)
 Frame = +2

Query: 98   MTSEVWKWCLGLTYXXXXXXXXXXXXXXXQSVVDAGVSPFLITYICNSLFVVYIPLVEVG 277
            M +EVW+W LGL Y               QSVVDAGVSPFLITYICNSLFV+YIPLVE+G
Sbjct: 1    MRTEVWRWVLGLIYIVAVASIWIAASYVVQSVVDAGVSPFLITYICNSLFVIYIPLVEIG 60

Query: 278  RYLEDTYGSLLFWRSGNNSSFQEVRESEEAILL-------RSDTKHQVADLKADEVE--R 430
            RYLED+YGSL FWRS  +   QE+ +SE+ +LL       R     Q   L+  E+   R
Sbjct: 61   RYLEDSYGSLCFWRSKKDIGLQEIGDSEQVVLLGESNLGARVGGSKQPVILEQGEISQHR 120

Query: 431  LECYSPEGLAMMR--TLESDQPGFFGNVENGLDGKGRWTRIRVAKVSLLICPFWFLAQLS 604
            +   S   L + R      DQ     + +  LD KGRWTR RVAK SLL+CPFWFLAQL+
Sbjct: 121  IGIVSELDLKVERFEAASLDQAIVRKDGDEQLDSKGRWTRTRVAKASLLVCPFWFLAQLT 180

Query: 605  FNLSLKYTTVTSNTILSSASSLFTFLVSLAFLGETFTLVKLISVLLCMGGTIIVSLGDSE 784
            FNLSL+YTTVTSNTILSSASSLFTFLV+L FLGE FT VKLISVLLCMGGTIIVSLGDSE
Sbjct: 181  FNLSLEYTTVTSNTILSSASSLFTFLVALVFLGEKFTWVKLISVLLCMGGTIIVSLGDSE 240

Query: 785  STLKAVASNPLLGDILALLSAAFYSVYITLIRKHIPDDDDENCGHVSMAQVLGFLGLSNL 964
            + L A+A+NPLLGDI AL+SAA Y+VYITLIRK+IPDDD ++ G VSMAQ LGFLGL NL
Sbjct: 241  TGLSAIATNPLLGDIFALVSAALYAVYITLIRKNIPDDDGKS-GQVSMAQFLGFLGLFNL 299

Query: 965  LIFLPVALVLNFTKLEPF 1018
            LIFLPVAL+LNFTKLEPF
Sbjct: 300  LIFLPVALILNFTKLEPF 317


>ref|XP_003622936.1| Solute carrier family 35 member F5 [Medicago truncatula]
            gi|355497951|gb|AES79154.1| Solute carrier family 35
            member F5 [Medicago truncatula]
          Length = 441

 Score =  361 bits (927), Expect = 1e-97
 Identities = 204/317 (64%), Positives = 232/317 (73%), Gaps = 7/317 (2%)
 Frame = +2

Query: 89   SKEMTSEVWKWCLGLTYXXXXXXXXXXXXXXXQSVVDAGVSPFLITYICNSLFVVYIPLV 268
            SK++ S+ WKW LGL Y               QSVVDAGVSPFL+TYICNSLFVV IP+V
Sbjct: 4    SKDLDSKAWKWGLGLIYIIAVAIIWIAASFVVQSVVDAGVSPFLVTYICNSLFVVLIPIV 63

Query: 269  EVGRYLEDTYGSLLFWRSGNNSSFQEVRESEEAILLRSD--TKHQVADLKADEVERL-EC 439
            E+GRYLED+YGSLLFW+S + S    + ESE+AILLR +  +   V  L  DEV+ + + 
Sbjct: 64   EIGRYLEDSYGSLLFWKS-DKSLKGRLGESEQAILLRDNEASGEVVESLVIDEVDGIVDR 122

Query: 440  YSPEGLAMMRTLESDQPGFFGNVEN----GLDGKGRWTRIRVAKVSLLICPFWFLAQLSF 607
                 L     +     G  G VEN    GLD KGRWTR RVAKVSLLICPFWF AQL+F
Sbjct: 123  NDGSELLPSDDVVGGLVGRVGLVENVDQKGLDEKGRWTRCRVAKVSLLICPFWFFAQLTF 182

Query: 608  NLSLKYTTVTSNTILSSASSLFTFLVSLAFLGETFTLVKLISVLLCMGGTIIVSLGDSES 787
            NLSLKYTTVTSNTILSSASSLFTFLVSLA LGE F  +KL SVLLCMGGTIIVSLGDS+S
Sbjct: 183  NLSLKYTTVTSNTILSSASSLFTFLVSLALLGEKFAWLKLFSVLLCMGGTIIVSLGDSQS 242

Query: 788  TLKAVASNPLLGDILALLSAAFYSVYITLIRKHIPDDDDENCGHVSMAQVLGFLGLSNLL 967
             L+ VASNPLLGDI AL SA  Y+VYITLIRK + DD+ +N G  SMAQ LGFLGL N+L
Sbjct: 243  GLRTVASNPLLGDIFALSSAGLYAVYITLIRKKLNDDEGKN-GEASMAQFLGFLGLFNVL 301

Query: 968  IFLPVALVLNFTKLEPF 1018
            +FLPVAL+LNFTK EPF
Sbjct: 302  LFLPVALILNFTKAEPF 318


>ref|XP_002882510.1| membrane protein [Arabidopsis lyrata subsp. lyrata]
            gi|297328350|gb|EFH58769.1| membrane protein [Arabidopsis
            lyrata subsp. lyrata]
          Length = 439

 Score =  360 bits (925), Expect = 2e-97
 Identities = 200/321 (62%), Positives = 231/321 (71%), Gaps = 13/321 (4%)
 Frame = +2

Query: 98   MTSEVWKWCLGLTYXXXXXXXXXXXXXXXQSVVDAGVSPFLITYICNSLFVVYIPLVEVG 277
            M++++W+W LGL Y               QSVV+AGVSPFLIT+ICNSLFVVY+PL E+G
Sbjct: 1    MSTKLWRWVLGLMYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIG 60

Query: 278  RYLEDTYGSLLFWRSGNNSSFQEVRESEEAILLRSDT---KHQVADLKADEVERLECYSP 448
            RYLED YGSLLFWRS   S   E+ ESE+A+LL  D    K    +  +  V R E  S 
Sbjct: 61   RYLEDAYGSLLFWRS-KRSHLMELVESEKAVLLGQDVSGVKSDATESSSGLVVREEEISE 119

Query: 449  EGLAMMRTLESDQPGFFGNVE----------NGLDGKGRWTRIRVAKVSLLICPFWFLAQ 598
             G  +   LE+ +    G+V            GLD KGRWTR+RVAKVSL+ICPFWFLAQ
Sbjct: 120  GGNGIESGLENVELEINGSVNVSDGASGVSNKGLDEKGRWTRMRVAKVSLVICPFWFLAQ 179

Query: 599  LSFNLSLKYTTVTSNTILSSASSLFTFLVSLAFLGETFTLVKLISVLLCMGGTIIVSLGD 778
            L+FN+SLKYTTVTSNTILSSASSLFTFLVSL FLGE FT +KL SVLLCM GTIIVS+GD
Sbjct: 180  LTFNVSLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIVSMGD 239

Query: 779  SESTLKAVASNPLLGDILALLSAAFYSVYITLIRKHIPDDDDENCGHVSMAQVLGFLGLS 958
            SES   AVA NPLLGDIL+L+SAA Y+VYITLIRK +PDDD+ N G VSMAQ LGFLGL 
Sbjct: 240  SESNSNAVAKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERN-GRVSMAQFLGFLGLF 298

Query: 959  NLLIFLPVALVLNFTKLEPFD 1021
            N  IFLP AL+LNFTK E F+
Sbjct: 299  NFFIFLPPALILNFTKRERFN 319


>ref|NP_187364.1| EamA-like transporter [Arabidopsis thaliana]
            gi|6642657|gb|AAF20238.1|AC012395_25 putative integral
            membrane protein [Arabidopsis thaliana]
            gi|30794019|gb|AAP40456.1| putative integral membrane
            protein [Arabidopsis thaliana] gi|53749190|gb|AAU90080.1|
            At3g07080 [Arabidopsis thaliana]
            gi|332640975|gb|AEE74496.1| EamA-like transporter
            [Arabidopsis thaliana]
          Length = 438

 Score =  358 bits (920), Expect = 9e-97
 Identities = 201/324 (62%), Positives = 233/324 (71%), Gaps = 16/324 (4%)
 Frame = +2

Query: 98   MTSEVWKWCLGLTYXXXXXXXXXXXXXXXQSVVDAGVSPFLITYICNSLFVVYIPLVEVG 277
            M++++W+W LGL Y               QSVV+AGVSPFLIT+ICNSLFVVY+PL E+G
Sbjct: 1    MSTKLWRWVLGLIYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIG 60

Query: 278  RYLEDTYGSLLFWRSGNNSSFQEVRESEEAILLRSDTKHQVADLKADEVE------RLEC 439
            RYLED YGSLLFWRS   S   E+ ESE+A+LL  D    V+ +K+D  E      R + 
Sbjct: 61   RYLEDAYGSLLFWRS-KRSHLMELVESEKAVLLGKD----VSGVKSDATESSGLVVREQE 115

Query: 440  YSPEGLAMMRTLESDQ----------PGFFGNVENGLDGKGRWTRIRVAKVSLLICPFWF 589
             S  G  +   LE+ +           G  G    GLD KGRWTR+RVAKVSL+ICPFWF
Sbjct: 116  ISEGGNGIESGLENIELEIDASVNVSDGASGVSNKGLDEKGRWTRMRVAKVSLVICPFWF 175

Query: 590  LAQLSFNLSLKYTTVTSNTILSSASSLFTFLVSLAFLGETFTLVKLISVLLCMGGTIIVS 769
            LAQL+FN+SLKYTTVTSNTILSSASSLFTFLVSL FLGE FT +KL SVLLCM GTIIVS
Sbjct: 176  LAQLTFNVSLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSGTIIVS 235

Query: 770  LGDSESTLKAVASNPLLGDILALLSAAFYSVYITLIRKHIPDDDDENCGHVSMAQVLGFL 949
            +GDSES   AVA NPLLGDIL+L+SAA Y+VYITLIRK +PDDD+ N G VSMAQ LGFL
Sbjct: 236  MGDSESKSNAVAKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERN-GRVSMAQFLGFL 294

Query: 950  GLSNLLIFLPVALVLNFTKLEPFD 1021
            GL N  IFLP AL+LNFTK E F+
Sbjct: 295  GLFNFFIFLPPALILNFTKRERFN 318


>ref|XP_002521105.1| conserved hypothetical protein [Ricinus communis]
            gi|223539674|gb|EEF41256.1| conserved hypothetical
            protein [Ricinus communis]
          Length = 430

 Score =  355 bits (912), Expect = 8e-96
 Identities = 201/322 (62%), Positives = 229/322 (71%), Gaps = 14/322 (4%)
 Frame = +2

Query: 98   MTSEVWKWCLGLTYXXXXXXXXXXXXXXXQSVVDAGVSPFLITYICNSLFVVYIPLVEVG 277
            M ++ W W +GL Y               QSVVDAGVSPFLITYICNSLFV+YIPLVE+G
Sbjct: 1    MENKAWGWIVGLIYIFAVAAIWIAASFVVQSVVDAGVSPFLITYICNSLFVIYIPLVEIG 60

Query: 278  RYLEDTYGSLLFWRSGNNSS-FQEVRESEEAILLRSDTKHQVADLKADE------VERLE 436
            RYLED YGSL FWR  +++S  QE+ ESE+AILL           KADE      +E + 
Sbjct: 61   RYLEDHYGSLFFWRKTSSTSPLQELGESEQAILLGETD----LGAKADELNPSIFIEEVN 116

Query: 437  CYSPEGL-------AMMRTLESDQPGFFGNVENGLDGKGRWTRIRVAKVSLLICPFWFLA 595
              SP G        +M  TL+   P   G    G+D KGRWTR RVAKVSLLICPFWF A
Sbjct: 117  S-SPHGNGVGAGVESMSSTLQRILP--VGETGKGVDTKGRWTRARVAKVSLLICPFWFFA 173

Query: 596  QLSFNLSLKYTTVTSNTILSSASSLFTFLVSLAFLGETFTLVKLISVLLCMGGTIIVSLG 775
            QL+FNLSLKYTTVTSNTILS+ASSLFTFLVSLAFLGE FT +KL SVLLCM GTIIVSLG
Sbjct: 174  QLTFNLSLKYTTVTSNTILSTASSLFTFLVSLAFLGEKFTWLKLASVLLCMAGTIIVSLG 233

Query: 776  DSESTLKAVASNPLLGDILALLSAAFYSVYITLIRKHIPDDDDENCGHVSMAQVLGFLGL 955
            DS++ + AVASNPLLGD  AL+SA  Y+ YITLIR   PDDD ++ GHVSMAQ LG+LGL
Sbjct: 234  DSKNGISAVASNPLLGDFFALISAGLYAAYITLIRLKFPDDDGKS-GHVSMAQFLGYLGL 292

Query: 956  SNLLIFLPVALVLNFTKLEPFD 1021
             NL +FLPV LVLNFT LEPF+
Sbjct: 293  FNLFLFLPVVLVLNFTMLEPFN 314