BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020548
         (815 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAS01523.1| putative beta-amyrin synthase [Centella asiatica]      466   e-129
gb|AFJ19235.1| mixed amyrin synthase [Catharanthus roseus]            463   e-128
gb|AEX99665.1| amyrin synthase [Catharanthus roseus]                  463   e-128
dbj|BAF63702.1| mixed amyrin synthase [Olea europaea]                 445   e-123
gb|AED99865.1| DS synthase [Panax notoginseng]                        441   e-121

>gb|AAS01523.1| putative beta-amyrin synthase [Centella asiatica]
          Length = 760

 Score =  466 bits (1200), Expect = e-129
 Identities = 212/271 (78%), Positives = 236/271 (87%)
 Frame = +1

Query: 1   FVGRQIWEFQPDAGTPEERQEVENARNVFRQNYINGVQHPCGDLFMRMQLIKENGIDVLS 180
           FVGRQIWE+ PDAGTPEER EVE  R  ++ N IN   HPCGD+ MR+QLIKE+G+D+LS
Sbjct: 20  FVGRQIWEYDPDAGTPEERLEVEKLRETYKYNLINNGIHPCGDMLMRLQLIKESGLDLLS 79

Query: 181 IPAARVAENEQVNYETATIAVKKAVRLNRAIQAHDGHWPAENAGPMFFTPPLIIALYISG 360
           IP  R+ E E+VNY+  T AVKKA+RLNRAIQAHDGHWPAENAGPMFFTPPLIIALYISG
Sbjct: 80  IPPVRLGEQEEVNYQVVTTAVKKALRLNRAIQAHDGHWPAENAGPMFFTPPLIIALYISG 139

Query: 361 AMDGVLTQEHKKEIIRFIYNHQNKDGGWGFYISGDSTMIGTALNYVALRLLGVGPNDGGG 540
           A+D  LT +HKKE+IRFIY HQNKDGGWGFYI G STMIG+AL+YVALRLLG GP+DG G
Sbjct: 140 AIDTHLTIQHKKEMIRFIYLHQNKDGGWGFYIEGHSTMIGSALSYVALRLLGEGPDDGDG 199

Query: 541 AVERGRKWILDHGSATSVPSWGKLFLSVLGVYEWEGCNPLPPEFWLYPTAFPYHPAKMWC 720
           AVER RKWILDHG A S+PSWGK +L+VLGVYEWEGCNPLPPEFWL+P A PYHPAKMWC
Sbjct: 200 AVERARKWILDHGGAASIPSWGKTYLAVLGVYEWEGCNPLPPEFWLFPEALPYHPAKMWC 259

Query: 721 YCRTTYMPMSYLYGRKYHGPITSLVIQLRQE 813
           YCRTTYMPMSYLYG+KYHGPIT LVI LR+E
Sbjct: 260 YCRTTYMPMSYLYGKKYHGPITDLVISLRKE 290


>gb|AFJ19235.1| mixed amyrin synthase [Catharanthus roseus]
          Length = 762

 Score =  463 bits (1191), Expect = e-128
 Identities = 207/271 (76%), Positives = 239/271 (88%)
 Frame = +1

Query: 1   FVGRQIWEFQPDAGTPEERQEVENARNVFRQNYINGVQHPCGDLFMRMQLIKENGIDVLS 180
           FVGRQIWE+ P+AGTP+ER+  E AR  FR N   GV +PC DLFMRMQLIKENGID++S
Sbjct: 20  FVGRQIWEYDPNAGTPQEREAFEKAREQFRNNRKKGVHNPCADLFMRMQLIKENGIDLMS 79

Query: 181 IPAARVAENEQVNYETATIAVKKAVRLNRAIQAHDGHWPAENAGPMFFTPPLIIALYISG 360
           IP  RV E E++ +E  TIAVKKA+RLNRAIQA DGHWPAENAGPMFFTPPL+IALYISG
Sbjct: 80  IPPVRVEEKEELTFEKTTIAVKKALRLNRAIQATDGHWPAENAGPMFFTPPLLIALYISG 139

Query: 361 AMDGVLTQEHKKEIIRFIYNHQNKDGGWGFYISGDSTMIGTALNYVALRLLGVGPNDGGG 540
           A++ +LT EHKKE++R+IYNHQN+DGGWGFYI G STMIG+AL+YVALRLLG GP+DG G
Sbjct: 140 AINTILTSEHKKELVRYIYNHQNEDGGWGFYIEGHSTMIGSALSYVALRLLGEGPDDGDG 199

Query: 541 AVERGRKWILDHGSATSVPSWGKLFLSVLGVYEWEGCNPLPPEFWLYPTAFPYHPAKMWC 720
           AV RGR+WILDHG AT +PSWGK +LSVLGVY+W+GCNPLPPEFWL+P+ FPYHPAKMWC
Sbjct: 200 AVGRGRQWILDHGGATGIPSWGKTYLSVLGVYDWDGCNPLPPEFWLFPSFFPYHPAKMWC 259

Query: 721 YCRTTYMPMSYLYGRKYHGPITSLVIQLRQE 813
           YCRTTYMPMSYLYG+KYHGP+T LV+QLRQE
Sbjct: 260 YCRTTYMPMSYLYGKKYHGPLTHLVMQLRQE 290


>gb|AEX99665.1| amyrin synthase [Catharanthus roseus]
          Length = 762

 Score =  463 bits (1191), Expect = e-128
 Identities = 207/271 (76%), Positives = 239/271 (88%)
 Frame = +1

Query: 1   FVGRQIWEFQPDAGTPEERQEVENARNVFRQNYINGVQHPCGDLFMRMQLIKENGIDVLS 180
           FVGRQIWE+ P+AGTP+ER+  E AR  FR N   GV +PC DLFMRMQLIKENGID++S
Sbjct: 20  FVGRQIWEYDPNAGTPQEREAFEKAREQFRNNRKKGVHNPCADLFMRMQLIKENGIDLMS 79

Query: 181 IPAARVAENEQVNYETATIAVKKAVRLNRAIQAHDGHWPAENAGPMFFTPPLIIALYISG 360
           IP  RV E E++ +E  TIAVKKA+RLNRAIQA DGHWPAENAGPMFFTPPL+IALYISG
Sbjct: 80  IPPVRVEEKEELTFEKTTIAVKKALRLNRAIQATDGHWPAENAGPMFFTPPLLIALYISG 139

Query: 361 AMDGVLTQEHKKEIIRFIYNHQNKDGGWGFYISGDSTMIGTALNYVALRLLGVGPNDGGG 540
           A++ +LT EHKKE++R+IYNHQN+DGGWGFYI G STMIG+AL+YVALRLLG GP+DG G
Sbjct: 140 AINTILTSEHKKELVRYIYNHQNEDGGWGFYIEGHSTMIGSALSYVALRLLGEGPDDGDG 199

Query: 541 AVERGRKWILDHGSATSVPSWGKLFLSVLGVYEWEGCNPLPPEFWLYPTAFPYHPAKMWC 720
           AV RGR+WILDHG AT +PSWGK +LSVLGVY+W+GCNPLPPEFWL+P+ FPYHPAKMWC
Sbjct: 200 AVGRGRQWILDHGGATGIPSWGKTYLSVLGVYDWDGCNPLPPEFWLFPSFFPYHPAKMWC 259

Query: 721 YCRTTYMPMSYLYGRKYHGPITSLVIQLRQE 813
           YCRTTYMPMSYLYG+KYHGP+T LV+QLRQE
Sbjct: 260 YCRTTYMPMSYLYGKKYHGPLTHLVMQLRQE 290


>dbj|BAF63702.1| mixed amyrin synthase [Olea europaea]
          Length = 762

 Score =  445 bits (1144), Expect = e-123
 Identities = 198/272 (72%), Positives = 234/272 (86%), Gaps = 1/272 (0%)
 Frame = +1

Query: 1   FVGRQIWEFQPDAGTPEERQEVENARNVFRQNY-INGVQHPCGDLFMRMQLIKENGIDVL 177
           F GRQIWE+ P+ GTPEER+  + AR  F++N  + GV HPCGDLFMR+QLIKE+GID++
Sbjct: 20  FAGRQIWEYDPNGGTPEEREAYDKAREEFQRNRKLKGV-HPCGDLFMRIQLIKESGIDLM 78

Query: 178 SIPAARVAENEQVNYETATIAVKKAVRLNRAIQAHDGHWPAENAGPMFFTPPLIIALYIS 357
           SIP  R+ E E+V YETAT AVKKA+ LNRA+QA DGHWPAENAGPMFFTPPLII LYIS
Sbjct: 79  SIPPVRLGEKEEVTYETATTAVKKALLLNRAVQASDGHWPAENAGPMFFTPPLIIVLYIS 138

Query: 358 GAMDGVLTQEHKKEIIRFIYNHQNKDGGWGFYISGDSTMIGTALNYVALRLLGVGPNDGG 537
           GA++ +LT EH+KE++R+IYNHQN DGGWGFYI G STMIG+AL+Y+ALRLLG GP+DG 
Sbjct: 139 GAINTILTSEHRKEMVRYIYNHQNDDGGWGFYIEGHSTMIGSALSYIALRLLGEGPDDGN 198

Query: 538 GAVERGRKWILDHGSATSVPSWGKLFLSVLGVYEWEGCNPLPPEFWLYPTAFPYHPAKMW 717
           G++ R RKWILDHG AT +PSWGK +LSVLGVY+W+GCNPLPPEFWL+P+  PYHPAKMW
Sbjct: 199 GSIARARKWILDHGGATGIPSWGKTYLSVLGVYDWDGCNPLPPEFWLFPSFLPYHPAKMW 258

Query: 718 CYCRTTYMPMSYLYGRKYHGPITSLVIQLRQE 813
           CYCRTTYMPMSYLYGRKYHGP+T LV+ LR E
Sbjct: 259 CYCRTTYMPMSYLYGRKYHGPLTDLVLSLRNE 290


>gb|AED99865.1| DS synthase [Panax notoginseng]
          Length = 769

 Score =  441 bits (1133), Expect = e-121
 Identities = 201/274 (73%), Positives = 232/274 (84%), Gaps = 3/274 (1%)
 Frame = +1

Query: 1   FVGRQIWEFQPDAGTPEERQEVENARNVFRQNYINGVQHPCGDLFMRMQLIKENGIDVLS 180
           FVGRQ WEFQPDAGTPEER+EVENAR  +  N      HPC D+ MR QLIKE+GID+LS
Sbjct: 20  FVGRQYWEFQPDAGTPEEREEVENARKDYVNNKKLHGXHPCSDMLMRRQLIKESGIDLLS 79

Query: 181 IPAARVAENEQVNYETATIAVKKAVRLNRAIQAHDGHWPAENAGPMFFTPPLIIALYISG 360
           IP  R+ ENEQVNY+  T AVKKA+RLNRAIQAHDGHWPAENAG + +TPPLIIALYISG
Sbjct: 80  IPPVRLDENEQVNYDAVTTAVKKALRLNRAIQAHDGHWPAENAGSLLYTPPLIIALYISG 139

Query: 361 AMDGVLTQEHKKEIIRFIYNHQNKDGGWGFYISGDSTMIGTALNYVALRLLGVG---PND 531
            +D +LT++HKKE+IRF+YNHQN+DGGWG YI G STMIG+ L++V LRLLG G    +D
Sbjct: 140 TIDTILTKQHKKELIRFVYNHQNEDGGWGSYIEGHSTMIGSVLSFVMLRLLGEGLAESDD 199

Query: 532 GGGAVERGRKWILDHGSATSVPSWGKLFLSVLGVYEWEGCNPLPPEFWLYPTAFPYHPAK 711
           G GAVERGRKWILDHG A S+PSWGK +L+VLGVYEWEGCNPLPPEFWL+P++FP+HPAK
Sbjct: 200 GNGAVERGRKWILDHGGAASIPSWGKTYLAVLGVYEWEGCNPLPPEFWLFPSSFPFHPAK 259

Query: 712 MWCYCRTTYMPMSYLYGRKYHGPITSLVIQLRQE 813
           MW YCR TYMPMSYLYG++YHGPIT LV+ LRQE
Sbjct: 260 MWIYCRCTYMPMSYLYGKRYHGPITDLVLSLRQE 293