BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020394
         (1514 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003634700.1| PREDICTED: uncharacterized protein LOC100241...   322   e-131
ref|XP_002316974.1| predicted protein [Populus trichocarpa] gi|2...   309   e-125
ref|XP_002319277.1| predicted protein [Populus trichocarpa] gi|2...   300   e-125
emb|CBI20510.3| unnamed protein product [Vitis vinifera]              322   e-122
ref|XP_004152911.1| PREDICTED: uncharacterized protein LOC101210...   293   e-117

>ref|XP_003634700.1| PREDICTED: uncharacterized protein LOC100241773 isoform 2 [Vitis
            vinifera]
          Length = 1215

 Score =  322 bits (824), Expect(2) = e-131
 Identities = 176/328 (53%), Positives = 218/328 (66%), Gaps = 2/328 (0%)
 Frame = -1

Query: 980  LHKFXXXXXXXXXXXXXXXXLEHLSVGPLSKLFKTSPLIGNEVKDNENGGAETGKSSTML 801
            LH+F                LE+LS GPLSKLFK SPL+ + ++  ENG    GK +  L
Sbjct: 856  LHRFGILGADGGPGEGLSKGLENLSAGPLSKLFKASPLLVDNLE--ENGSYRDGKDNGFL 913

Query: 800  HLGTPDDVDVSIELKNWLFALEGAEEMAEMWWFGDSKDSSRAQRCWHTTFRSLQVKAKGQ 621
            +LG PDDVDVSIELK+WLFALEGA+E AE WWF + ++  R +RCWHTTF+SLQVKAKG 
Sbjct: 914  NLGKPDDVDVSIELKDWLFALEGAQETAERWWFYNDENIGREERCWHTTFQSLQVKAKGS 973

Query: 620  GNLLNTARPNGRK--KHPIELITIIADGVKTLKPQPRKTIQQNSEPAKGFKQNAEAADGI 447
               L   +   ++  K+P+ELIT+  +G++ LKP   K I Q   P +G K+  E + GI
Sbjct: 974  PKRLLNGKGKSQETQKYPVELITVGIEGLQILKPNAAKGILQAGFPVEGIKETVETSGGI 1033

Query: 446  NFIFDMVICEDETDVATSKWEVENMKFSVKQPFEAIMTKDELQNLTLLCKSEVDSMGRIT 267
            N    +++ ED       KW VEN+KFSVKQP EAI+TKDELQ L  LCKSEVDSMGRI 
Sbjct: 1034 NCEVSILVSEDNAHDEIGKWMVENLKFSVKQPIEAIVTKDELQYLAFLCKSEVDSMGRIA 1093

Query: 266  AGVLRVLKLEGSVGQAAITQLSNLGSEGFDKMFSTYKPNSSRTEGSYVVPPSDVVGGSWN 87
            AG+LRVLKLEGSVGQAAI QLSNLG+EGFDK+FS    +      +    P++  G S +
Sbjct: 1094 AGILRVLKLEGSVGQAAIDQLSNLGTEGFDKIFSPEILSPHSYASNIGFTPANGNGQSPH 1153

Query: 86   PRLESAVASLEEAVLDSQATCAILTAKM 3
            P LES V SLEEAVLDSQA C  L A++
Sbjct: 1154 PSLESTVFSLEEAVLDSQAKCTALIAEL 1181



 Score =  176 bits (446), Expect(2) = e-131
 Identities = 88/147 (59%), Positives = 112/147 (76%), Gaps = 4/147 (2%)
 Frame = -2

Query: 1510 EKAPGDTAVSLFCKDIHLRFLESTSIDMLGMPLVHFVGEDLFVKVGHRTLGGAVAISTNL 1331
            EK P DTAVSL  KD+ L+FLES+S+D+  MPLV FVG+DLF+KV HRTLGGA+AIS+ L
Sbjct: 690  EKVPSDTAVSLAVKDLQLQFLESSSMDIHEMPLVQFVGDDLFIKVTHRTLGGAIAISSTL 749

Query: 1330 NWERVEVECADIERNFGSENGVVLPSVENGHLN-GNGYPQLRAVFWVQNRNNSQSN---D 1163
            +W  VE++C D E N   ENG  L S ENG L+ G+G PQLR VFWVQN+   +SN    
Sbjct: 750  HWGSVEIDCVDTEGNLLHENGTTLTSTENGLLSAGSGSPQLRPVFWVQNKWKHRSNGIAH 809

Query: 1162 TVPFLDITMVHVIPYSSEDMECHSLNV 1082
             +P LDI++VHVIPY+++D+ECHSL+V
Sbjct: 810  AIPLLDISVVHVIPYNAQDIECHSLSV 836


>ref|XP_002316974.1| predicted protein [Populus trichocarpa] gi|222860039|gb|EEE97586.1|
            predicted protein [Populus trichocarpa]
          Length = 809

 Score =  309 bits (791), Expect(2) = e-125
 Identities = 171/325 (52%), Positives = 213/325 (65%), Gaps = 3/325 (0%)
 Frame = -1

Query: 980  LHKFXXXXXXXXXXXXXXXXLEHLSVGPLSKLFKTSPLIGNEVKDNENGGAETGKSSTML 801
            LH+F                LE+LS GPLSKLFK SPLI     DN   G        +L
Sbjct: 455  LHRFGVLGPDGGPGEGLSKGLENLSTGPLSKLFKGSPLI-----DNLKEGIPVDGKDGVL 509

Query: 800  HLGTPDDVDVSIELKNWLFALEGAEEMAEMWWFGDSKDSSRAQRCWHTTFRSLQVKAKG- 624
            HLG PDDVDV IE K+WLFALEGA+EM + WWF + +D  R +RCWHT+F+SL VKAK  
Sbjct: 510  HLGIPDDVDVCIEFKDWLFALEGAQEMTDRWWFYNHEDVGREERCWHTSFQSLLVKAKSG 569

Query: 623  -QGNLLNTARPNGRKKHPIELITIIADGVKTLKPQPRKTIQQNSEPAKGFKQNAEAADGI 447
             +       +PNG+ K+P+EL+T+  +G++TLKPQ +K +   S PA G K+  E + G+
Sbjct: 570  PKKERNGKGKPNGKLKYPVELVTVGVEGLQTLKPQGQKGV---SMPANGIKEVVETSGGV 626

Query: 446  NFIFDMVICEDETDVATSKWEVENMKFSVKQPFEAIMTKDELQNLTLLCKSEVDSMGRIT 267
            N    MV  E+  D   + W VEN+KFSVKQP EA++TKDELQ+L LLCKSEVD+MGRI 
Sbjct: 627  NLEVCMVALEENIDDEMANWAVENLKFSVKQPIEAVVTKDELQHLALLCKSEVDAMGRIA 686

Query: 266  AGVLRVLKLEGSVGQAAITQLSNLGSEGFDKMFSTYKPNSSRTEGSYVVPPS-DVVGGSW 90
            AGVL++LKLEGS+GQAAI QLSNLGSEGFDK+F+  K     +  S    PS  ++  S 
Sbjct: 687  AGVLKLLKLEGSIGQAAIDQLSNLGSEGFDKIFTPDKFRKGTSPASTSFSPSPHIINESP 746

Query: 89   NPRLESAVASLEEAVLDSQATCAIL 15
               +ES VASLEEAVLDSQA  A L
Sbjct: 747  RTTVESTVASLEEAVLDSQAKLAAL 771



 Score =  169 bits (427), Expect(2) = e-125
 Identities = 86/147 (58%), Positives = 108/147 (73%), Gaps = 4/147 (2%)
 Frame = -2

Query: 1510 EKAPGDTAVSLFCKDIHLRFLESTSIDMLGMPLVHFVGEDLFVKVGHRTLGGAVAISTNL 1331
            +K P DTAVSL  K++ LRFLES++ D+ GMPLV F+GEDLF+KV HRTLGGA+AIS+++
Sbjct: 289  DKVPCDTAVSLAVKELRLRFLESSASDIEGMPLVQFIGEDLFIKVAHRTLGGAIAISSSI 348

Query: 1330 NWERVEVECADIERNFGSENGVVLPSVENGHL-NGNGYPQLRAVFWVQNRNNSQSND--- 1163
             W+ VEV+C + E +   ENG    SVENG L   N YP+LRAVFWV N +  Q+N    
Sbjct: 349  CWQSVEVDCVETEGSLTYENGTQTSSVENGCLVAANKYPELRAVFWVHNGHKYQANGITR 408

Query: 1162 TVPFLDITMVHVIPYSSEDMECHSLNV 1082
            T+PFLD +MVHVIP S  D ECHSL+V
Sbjct: 409  TIPFLDTSMVHVIPLSELDRECHSLSV 435


>ref|XP_002319277.1| predicted protein [Populus trichocarpa] gi|222857653|gb|EEE95200.1|
            predicted protein [Populus trichocarpa]
          Length = 810

 Score =  300 bits (767), Expect(2) = e-125
 Identities = 171/325 (52%), Positives = 216/325 (66%), Gaps = 3/325 (0%)
 Frame = -1

Query: 980  LHKFXXXXXXXXXXXXXXXXLEHLSVGPLSKLFKTSPLIGNEVKDNENGGAETGKSSTML 801
            LH+F                LE+LS GPLSKLFK SPLI N     E+G    GK   +L
Sbjct: 455  LHRFGILGPDGGPGEGLSKGLENLSTGPLSKLFKGSPLIDNL---KEDGSLIDGKDG-VL 510

Query: 800  HLGTPDDVDVSIELKNWLFALEGAEEMAEMWWFGDSKDSSRAQRCWHTTFRSLQVKAKGQ 621
            HL  PDDVDV IELK+WLFALEGA+EMA  W+F +++D  R +RCWH +F+SLQ+KAK  
Sbjct: 511  HLRLPDDVDVCIELKDWLFALEGAQEMAGGWFFYNNEDVGREERCWHASFQSLQLKAKSS 570

Query: 620  GNL-LN-TARPNGRKKHPIELITIIADGVKTLKPQPRKTIQQNSEPAKGFKQNAEAADGI 447
              + LN   +PNG+ K+P+EL+T+  +G++TLKPQ +K I   S PA G K+  E + GI
Sbjct: 571  PKIELNGKEKPNGKLKYPVELVTVGVEGLQTLKPQGQKGI---STPANGIKEVVETSGGI 627

Query: 446  NFIFDMVICEDETDVATSKWEVENMKFSVKQPFEAIMTKDELQNLTLLCKSEVDSMGRIT 267
            N    MV  E+  D   +KW VEN+KFSVKQP EA++TKDE Q+L LLCKSEVD+MGRI 
Sbjct: 628  NLEVRMVASEENIDDEMAKWAVENLKFSVKQPIEAVVTKDEFQHLALLCKSEVDAMGRIA 687

Query: 266  AGVLRVLKLEGSVGQAAITQLSNLGSEGFDKMFSTYKPNSSRTEGSYVVPPSD-VVGGSW 90
            AG LR+LK E S+GQ+AI QLSNLGSEGFDK+F+  + +   +  S    PS  +V  S 
Sbjct: 688  AGFLRLLKFERSIGQSAIDQLSNLGSEGFDKIFTPDRLSRGASPASIAFSPSSYLVNESP 747

Query: 89   NPRLESAVASLEEAVLDSQATCAIL 15
               +ES V SLEEA+LDSQA  A L
Sbjct: 748  QTTMESTVTSLEEALLDSQAKLAAL 772



 Score =  176 bits (445), Expect(2) = e-125
 Identities = 85/147 (57%), Positives = 110/147 (74%), Gaps = 4/147 (2%)
 Frame = -2

Query: 1510 EKAPGDTAVSLFCKDIHLRFLESTSIDMLGMPLVHFVGEDLFVKVGHRTLGGAVAISTNL 1331
            +K P DTAVSL  K++ LRFLES++ D+ GMPLV F+GEDL++KV HRTLGGA+ IS+++
Sbjct: 289  DKVPCDTAVSLAVKELRLRFLESSASDIEGMPLVQFIGEDLYIKVSHRTLGGAIVISSSV 348

Query: 1330 NWERVEVECADIERNFGSENGVVLPSVENGHL-NGNGYPQLRAVFWVQNRNNSQSND--- 1163
             W+ VEV+C + E +   ENG++  SVENG L   NGYPQLRAVFWV N    Q+N    
Sbjct: 349  YWQSVEVDCVETEGSLAHENGMLTSSVENGRLVTANGYPQLRAVFWVHNGQKYQANGIAC 408

Query: 1162 TVPFLDITMVHVIPYSSEDMECHSLNV 1082
            T+PFLD +MVH+IP S +D ECHSL+V
Sbjct: 409  TIPFLDTSMVHMIPLSEQDQECHSLSV 435


>emb|CBI20510.3| unnamed protein product [Vitis vinifera]
          Length = 1146

 Score =  322 bits (824), Expect(2) = e-122
 Identities = 176/328 (53%), Positives = 218/328 (66%), Gaps = 2/328 (0%)
 Frame = -1

Query: 980  LHKFXXXXXXXXXXXXXXXXLEHLSVGPLSKLFKTSPLIGNEVKDNENGGAETGKSSTML 801
            LH+F                LE+LS GPLSKLFK SPL+ + ++  ENG    GK +  L
Sbjct: 787  LHRFGILGADGGPGEGLSKGLENLSAGPLSKLFKASPLLVDNLE--ENGSYRDGKDNGFL 844

Query: 800  HLGTPDDVDVSIELKNWLFALEGAEEMAEMWWFGDSKDSSRAQRCWHTTFRSLQVKAKGQ 621
            +LG PDDVDVSIELK+WLFALEGA+E AE WWF + ++  R +RCWHTTF+SLQVKAKG 
Sbjct: 845  NLGKPDDVDVSIELKDWLFALEGAQETAERWWFYNDENIGREERCWHTTFQSLQVKAKGS 904

Query: 620  GNLLNTARPNGRK--KHPIELITIIADGVKTLKPQPRKTIQQNSEPAKGFKQNAEAADGI 447
               L   +   ++  K+P+ELIT+  +G++ LKP   K I Q   P +G K+  E + GI
Sbjct: 905  PKRLLNGKGKSQETQKYPVELITVGIEGLQILKPNAAKGILQAGFPVEGIKETVETSGGI 964

Query: 446  NFIFDMVICEDETDVATSKWEVENMKFSVKQPFEAIMTKDELQNLTLLCKSEVDSMGRIT 267
            N    +++ ED       KW VEN+KFSVKQP EAI+TKDELQ L  LCKSEVDSMGRI 
Sbjct: 965  NCEVSILVSEDNAHDEIGKWMVENLKFSVKQPIEAIVTKDELQYLAFLCKSEVDSMGRIA 1024

Query: 266  AGVLRVLKLEGSVGQAAITQLSNLGSEGFDKMFSTYKPNSSRTEGSYVVPPSDVVGGSWN 87
            AG+LRVLKLEGSVGQAAI QLSNLG+EGFDK+FS    +      +    P++  G S +
Sbjct: 1025 AGILRVLKLEGSVGQAAIDQLSNLGTEGFDKIFSPEILSPHSYASNIGFTPANGNGQSPH 1084

Query: 86   PRLESAVASLEEAVLDSQATCAILTAKM 3
            P LES V SLEEAVLDSQA C  L A++
Sbjct: 1085 PSLESTVFSLEEAVLDSQAKCTALIAEL 1112



 Score =  144 bits (362), Expect(2) = e-122
 Identities = 75/146 (51%), Positives = 97/146 (66%), Gaps = 3/146 (2%)
 Frame = -2

Query: 1510 EKAPGDTAVSLFCKDIHLRFLESTSIDMLGMPLVHFVGEDLFVKVGHRTLGGAVAISTNL 1331
            EK P DTAVSL  KD+ L+FLES+S+D+  MPLV FVG+DLF+KV HRTLGGA+AIS+ L
Sbjct: 644  EKVPSDTAVSLAVKDLQLQFLESSSMDIHEMPLVQFVGDDLFIKVTHRTLGGAIAISSTL 703

Query: 1330 NWERVEVECADIERNFGSENGVVLPSVENGHLNGNGYPQLRAVFWVQNRNNSQSN---DT 1160
            +W  VE++C D E N   EN                      VFWVQN+   +SN     
Sbjct: 704  HWGSVEIDCVDTEGNLLHEN----------------------VFWVQNKWKHRSNGIAHA 741

Query: 1159 VPFLDITMVHVIPYSSEDMECHSLNV 1082
            +P LDI++VHVIPY+++D+ECHSL+V
Sbjct: 742  IPLLDISVVHVIPYNAQDIECHSLSV 767


>ref|XP_004152911.1| PREDICTED: uncharacterized protein LOC101210396 [Cucumis sativus]
          Length = 1203

 Score =  293 bits (751), Expect(2) = e-117
 Identities = 158/329 (48%), Positives = 210/329 (63%), Gaps = 3/329 (0%)
 Frame = -1

Query: 980  LHKFXXXXXXXXXXXXXXXXLEHLSVGPLSKLFKTSPLIGNEVKDNENGGAETGKSSTML 801
            LH+F                LE+L  GPL KLFKTSPL+   ++ +       GK S++L
Sbjct: 849  LHRFGILGPDGGPGKGLMKGLENLRAGPLVKLFKTSPLLTGNLEGD-------GKESSLL 901

Query: 800  HLGTPDDVDVSIELKNWLFALEGAEEMAEMWWFGDSKDSSRAQRCWHTTFRSLQVKAKGQ 621
             LG PDDVDVSIELKNWLFALEGA+EMAE WWF +  ++ R +RCWHT+F+S +VKA+ +
Sbjct: 902  QLGKPDDVDVSIELKNWLFALEGAQEMAERWWFYNPNNAGREERCWHTSFQSFRVKAQSR 961

Query: 620  GN--LLNTARPNGRKKHPIELITIIADGVKTLKPQPRKTIQQNSEPAKGFKQNAEAADGI 447
                L       G ++ P+EL+ +  +G++TLKP  +K    N     G  +  E   GI
Sbjct: 962  RKEPLSGKGSSRGTQQFPVELVILSVEGLQTLKPHVQKNSHHNVSLINGVNETIEPLGGI 1021

Query: 446  NFIFDMVICEDETDVATSKWEVENMKFSVKQPFEAIMTKDELQNLTLLCKSEVDSMGRIT 267
            +    MV+ ED  DV  + W +EN+KFSVK P EA++TK+ELQ+L LL KSEVDSMGRI 
Sbjct: 1022 SLEARMVVSEDNVDVEMANWIMENLKFSVKHPIEAVVTKNELQHLALLFKSEVDSMGRIA 1081

Query: 266  AGVLRVLKLEGSVGQAAITQLSNLGSEGFDKMFSTYKPNSSRTEGSYVVPPSD-VVGGSW 90
            AG+LR+LKLEGS+GQA + QLSNLGSE  DK+F+  K +   +  S  V PS  ++G S 
Sbjct: 1082 AGILRLLKLEGSIGQATLDQLSNLGSESIDKIFTPEKLSRGSSMASLGVSPSAYLIGESP 1141

Query: 89   NPRLESAVASLEEAVLDSQATCAILTAKM 3
             P +ES V SLE+AVLDSQ+ C  L  ++
Sbjct: 1142 RPTIESTVTSLEQAVLDSQSKCTSLMTEL 1170



 Score =  157 bits (397), Expect(2) = e-117
 Identities = 77/144 (53%), Positives = 101/144 (70%), Gaps = 1/144 (0%)
 Frame = -2

Query: 1510 EKAPGDTAVSLFCKDIHLRFLESTSIDMLGMPLVHFVGEDLFVKVGHRTLGGAVAISTNL 1331
            +K P DTAVSL  +++ LRFLES+S  +  +PLV FVG D+F+KV HRTLGGAVAI++ +
Sbjct: 689  DKVPSDTAVSLLVRNLQLRFLESSSTIIEELPLVQFVGNDMFIKVSHRTLGGAVAITSTV 748

Query: 1330 NWERVEVECADIERNFGSENGVVLPSVENGHL-NGNGYPQLRAVFWVQNRNNSQSNDTVP 1154
             W+ VEV+C D E N   +NG +  S+ENG L  GN   QLRA+ WV N+ +       P
Sbjct: 749  RWDNVEVDCVDTEGNTAYDNGTMSTSIENGSLMKGNELSQLRAILWVHNKGD---RFPTP 805

Query: 1153 FLDITMVHVIPYSSEDMECHSLNV 1082
            FLD+++VHVIP +  DMECHSLNV
Sbjct: 806  FLDVSIVHVIPLNERDMECHSLNV 829