BLASTX nr result
ID: Bupleurum21_contig00020285
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00020285
(1176 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002521699.1| AMP dependent ligase, putative [Ricinus comm... 637 e-180
ref|XP_002521696.1| AMP dependent ligase, putative [Ricinus comm... 630 e-178
ref|XP_002307202.1| acyl:coa ligase acetate-coa synthetase-like ... 618 e-175
ref|XP_004163400.1| PREDICTED: LOW QUALITY PROTEIN: probable acy... 595 e-168
ref|XP_004134830.1| PREDICTED: probable acyl-activating enzyme 2... 595 e-168
>ref|XP_002521699.1| AMP dependent ligase, putative [Ricinus communis]
gi|223539090|gb|EEF40686.1| AMP dependent ligase,
putative [Ricinus communis]
Length = 544
Score = 637 bits (1643), Expect = e-180
Identities = 291/368 (79%), Positives = 328/368 (89%)
Frame = +1
Query: 1 RRPKTEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATTFLHGMGSMPVYLWTVPMFHC 180
RRPK EWDPIS+NYTSGTTSRPKGVVYSHRGAYLNSLAT FLHG+G+MPVYLWTVPMFHC
Sbjct: 177 RRPKNEWDPISVNYTSGTTSRPKGVVYSHRGAYLNSLATVFLHGIGAMPVYLWTVPMFHC 236
Query: 181 NGWCLTWGLAALGGTNVCLRRVCPKDIYDNIDLHKVTHMGGAPTVLNMIVNAQSCDKKTL 360
NGWCLTWG+AA GGTN+C+R+V PK I+D+I H VTHMGGAPTVLNMIVN+ D++TL
Sbjct: 237 NGWCLTWGVAAQGGTNICIRKVTPKAIFDSIGQHNVTHMGGAPTVLNMIVNSPVSDRRTL 296
Query: 361 PHKVDIMTGGSPPPPHILLKMEELGFRVSHLYGLTETYGPGTYCIWRPEWDSLPKEEQVK 540
PHKV+IMTGG+PPPP I+ KMEELGF VSHLYGLTETYGPGTYC W+PEWDSLP +E+ K
Sbjct: 297 PHKVEIMTGGAPPPPQIIFKMEELGFGVSHLYGLTETYGPGTYCAWKPEWDSLPPDERAK 356
Query: 541 LKARQGMHHFCLEDIDVKDPETMVNVPADGKTFGEIMFKGNTVMSGYFKDEKATQELLKV 720
LKARQG+HH LED+DV+DP T +VPADGKT GEI+F+GNTVMSGY KD KAT+E +
Sbjct: 357 LKARQGIHHLGLEDVDVRDPMTQESVPADGKTIGEIVFRGNTVMSGYLKDLKATEEAFQG 416
Query: 721 GWFRSGDLTVKHQDGYLEIKDRLKDVIISGDENISTVEVETVLYSHPAILEAAVVARPDN 900
GWFRSGDL VKH DGY+E+KDRLKD+IISG ENISTVEVETVLYSHPAI EAAVVARPD+
Sbjct: 417 GWFRSGDLAVKHPDGYIEVKDRLKDIIISGGENISTVEVETVLYSHPAIFEAAVVARPDD 476
Query: 901 HWGQTPCAFVKLKEGFQIDAEGIIKFCRDNLPHYMAPRTVVFEELPRTSTGKVQKFVLRE 1080
HWGQTPCAFVKLKEGF + + IIKFCRD LPHYMAPRTV+FE+LPRTSTGKVQKF+LR+
Sbjct: 477 HWGQTPCAFVKLKEGFVVSEQDIIKFCRDRLPHYMAPRTVIFEDLPRTSTGKVQKFILRQ 536
Query: 1081 KTKAMGSL 1104
K KA +L
Sbjct: 537 KAKATENL 544
>ref|XP_002521696.1| AMP dependent ligase, putative [Ricinus communis]
gi|223539087|gb|EEF40683.1| AMP dependent ligase,
putative [Ricinus communis]
Length = 480
Score = 630 bits (1626), Expect = e-178
Identities = 286/368 (77%), Positives = 326/368 (88%)
Frame = +1
Query: 1 RRPKTEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATTFLHGMGSMPVYLWTVPMFHC 180
RRPK+EWDPI++NYTSGTTSRPKGVV+SHRGAYLNSLAT LHG G+MPVYLWTVPMFH
Sbjct: 113 RRPKSEWDPITVNYTSGTTSRPKGVVFSHRGAYLNSLATVLLHGTGAMPVYLWTVPMFHS 172
Query: 181 NGWCLTWGLAALGGTNVCLRRVCPKDIYDNIDLHKVTHMGGAPTVLNMIVNAQSCDKKTL 360
NGWCLTWG+AA GGTN+C+R+V P I+D+I H VTHMGGAPTVLNMIVN+ D++TL
Sbjct: 173 NGWCLTWGMAAQGGTNICIRKVTPNAIFDSIGQHNVTHMGGAPTVLNMIVNSPVSDRRTL 232
Query: 361 PHKVDIMTGGSPPPPHILLKMEELGFRVSHLYGLTETYGPGTYCIWRPEWDSLPKEEQVK 540
PHKV+IMTGG+PPPP I+ KMEELGF VSHLYGLTETYGPGTYC W+PEWDSLP +E+ K
Sbjct: 233 PHKVEIMTGGAPPPPQIIFKMEELGFGVSHLYGLTETYGPGTYCAWKPEWDSLPPDERAK 292
Query: 541 LKARQGMHHFCLEDIDVKDPETMVNVPADGKTFGEIMFKGNTVMSGYFKDEKATQELLKV 720
+KARQG+HH LED+DV+DP T +VPADGKT GE++F+GNT+MSGY KD KAT+E +
Sbjct: 293 VKARQGIHHLGLEDVDVRDPMTQESVPADGKTIGEVVFRGNTIMSGYLKDLKATEEAFQG 352
Query: 721 GWFRSGDLTVKHQDGYLEIKDRLKDVIISGDENISTVEVETVLYSHPAILEAAVVARPDN 900
GWFRSGDL VKH DGY+E+KDRLKD+IISG ENISTVEVETVLYSHPAI EAAVVARPD+
Sbjct: 353 GWFRSGDLAVKHPDGYIEVKDRLKDIIISGGENISTVEVETVLYSHPAIFEAAVVARPDD 412
Query: 901 HWGQTPCAFVKLKEGFQIDAEGIIKFCRDNLPHYMAPRTVVFEELPRTSTGKVQKFVLRE 1080
HWGQTPCAFVKLKEGF + + IIKFCRD LPHYMAPRTV+FE+LPRTSTGKVQKF+LRE
Sbjct: 413 HWGQTPCAFVKLKEGFVVSEQDIIKFCRDRLPHYMAPRTVIFEDLPRTSTGKVQKFILRE 472
Query: 1081 KTKAMGSL 1104
K K MGSL
Sbjct: 473 KAKTMGSL 480
>ref|XP_002307202.1| acyl:coa ligase acetate-coa synthetase-like protein [Populus
trichocarpa] gi|222856651|gb|EEE94198.1| acyl:coa ligase
acetate-coa synthetase-like protein [Populus trichocarpa]
Length = 570
Score = 618 bits (1594), Expect = e-175
Identities = 280/368 (76%), Positives = 323/368 (87%)
Frame = +1
Query: 1 RRPKTEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATTFLHGMGSMPVYLWTVPMFHC 180
R+P++EWDPIS+NYTSGTTSRPKGVVYSHRGAYLN+LAT FLHG+G+ PVYLWTVPMFHC
Sbjct: 203 RQPESEWDPISVNYTSGTTSRPKGVVYSHRGAYLNTLATLFLHGIGTTPVYLWTVPMFHC 262
Query: 181 NGWCLTWGLAALGGTNVCLRRVCPKDIYDNIDLHKVTHMGGAPTVLNMIVNAQSCDKKTL 360
NGWCLTWG+AA GG NVCLR+V PKDI+D+ID HKVTHM GAPTVL+MIVN+ DKK L
Sbjct: 263 NGWCLTWGMAAQGGANVCLRKVSPKDIFDSIDQHKVTHMAGAPTVLSMIVNSAVSDKKPL 322
Query: 361 PHKVDIMTGGSPPPPHILLKMEELGFRVSHLYGLTETYGPGTYCIWRPEWDSLPKEEQVK 540
PHKV+IMTGG+PPPP I KME+LGF VSHLYGLTETYGPGTYC W+PEWDSLP E+ K
Sbjct: 323 PHKVEIMTGGAPPPPQIFFKMEKLGFGVSHLYGLTETYGPGTYCSWKPEWDSLPLNERSK 382
Query: 541 LKARQGMHHFCLEDIDVKDPETMVNVPADGKTFGEIMFKGNTVMSGYFKDEKATQELLKV 720
+KARQG+ H LED+DVKD TM +VPADGKT GEIM +GNTVMSGY KD KAT++ +
Sbjct: 383 MKARQGVQHLGLEDVDVKDTFTMESVPADGKTIGEIMLRGNTVMSGYLKDSKATEDAFRG 442
Query: 721 GWFRSGDLTVKHQDGYLEIKDRLKDVIISGDENISTVEVETVLYSHPAILEAAVVARPDN 900
GWFRSGDL VKH DGY+E+KDR KD++I+G EN+ T+EVETVLY+HPAILE AVV RPD+
Sbjct: 443 GWFRSGDLAVKHSDGYIEVKDRAKDIVITGGENVCTLEVETVLYNHPAILEVAVVGRPDD 502
Query: 901 HWGQTPCAFVKLKEGFQIDAEGIIKFCRDNLPHYMAPRTVVFEELPRTSTGKVQKFVLRE 1080
WGQTPCAFVKL+EGF +DA+ IIKFCRD LPHYMAP+TV+FE+LPR STGKVQKF+LRE
Sbjct: 503 LWGQTPCAFVKLREGFDVDAQDIIKFCRDRLPHYMAPKTVIFEDLPRNSTGKVQKFILRE 562
Query: 1081 KTKAMGSL 1104
K KA+GSL
Sbjct: 563 KAKALGSL 570
>ref|XP_004163400.1| PREDICTED: LOW QUALITY PROTEIN: probable acyl-activating enzyme
2-like [Cucumis sativus]
Length = 547
Score = 595 bits (1534), Expect = e-168
Identities = 269/370 (72%), Positives = 319/370 (86%), Gaps = 1/370 (0%)
Frame = +1
Query: 1 RRPKTEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATTFLHGMGSMPVYLWTVPMFHC 180
RRPK+EWDPISINYTSGTTS PKGVVYSHRGAYLNSLAT L GMGSMPVYLWT PMFHC
Sbjct: 178 RRPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHC 237
Query: 181 NGWCLTWGLAALGGTNVCLRRVCPKDIYDNIDLHKVTHMGGAPTVLNMIVNAQSCDKKTL 360
NGWCLTWG+AA GGTN+CLR+V PK I++ I LH THM APTVLNMI N+ D++ L
Sbjct: 238 NGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLL 297
Query: 361 PHKVDIMTGGSPPPPHILLKMEELGFRVSHLYGLTETYGPGTYCIWRPEWDSLPKEEQVK 540
P+KVD++TGGSPPPP +L KMEE+GFR+ HLYGLTETYGPGT C W+P WD LP +E+ +
Sbjct: 298 PNKVDVLTGGSPPPPQVLAKMEEMGFRICHLYGLTETYGPGTCCTWKPMWDDLPSDEKSR 357
Query: 541 LKARQGMHHFCLEDIDVKDPETMVNVPADGKTFGEIMFKGNTVMSGYFKDEKATQELLKV 720
++ARQG+HH L+++DV+DP+TM +VP DGKT GEIMF+GNTVMSGYFK++KAT+E K
Sbjct: 358 IRARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFKG 417
Query: 721 GWFRSGDLTVKHQDGYLEIKDRLKDVIISGDENISTVEVETVLYSHPAILEAAVVARPDN 900
GWF SGD VKH D Y+E+KDRLKDVIISG ENISTVEVET+L+SHP++LEAAVVARPD+
Sbjct: 418 GWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDD 477
Query: 901 HWGQTPCAFVKLKEGFQ-IDAEGIIKFCRDNLPHYMAPRTVVFEELPRTSTGKVQKFVLR 1077
HWG+TPCAFV LKEG + A+ +I +CRD LPHYMAPRT+VF++LP+TSTGKVQKF+LR
Sbjct: 478 HWGETPCAFVVLKEGCNTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILR 537
Query: 1078 EKTKAMGSLS 1107
E+ KAMGSLS
Sbjct: 538 ERAKAMGSLS 547
>ref|XP_004134830.1| PREDICTED: probable acyl-activating enzyme 2-like [Cucumis sativus]
Length = 547
Score = 595 bits (1533), Expect = e-168
Identities = 268/370 (72%), Positives = 319/370 (86%), Gaps = 1/370 (0%)
Frame = +1
Query: 1 RRPKTEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATTFLHGMGSMPVYLWTVPMFHC 180
RRPK+EWDPISINYTSGTTS PKGVVYSHRGAYLNSLAT L GMGSMPVYLWT PMFHC
Sbjct: 178 RRPKSEWDPISINYTSGTTSMPKGVVYSHRGAYLNSLATVLLGGMGSMPVYLWTCPMFHC 237
Query: 181 NGWCLTWGLAALGGTNVCLRRVCPKDIYDNIDLHKVTHMGGAPTVLNMIVNAQSCDKKTL 360
NGWCLTWG+AA GGTN+CLR+V PK I++ I LH THM APTVLNMI N+ D++ L
Sbjct: 238 NGWCLTWGVAAQGGTNLCLRKVSPKAIFEKIALHNATHMAAAPTVLNMIANSPVSDRRLL 297
Query: 361 PHKVDIMTGGSPPPPHILLKMEELGFRVSHLYGLTETYGPGTYCIWRPEWDSLPKEEQVK 540
P+KVD++TGGSPPPP +L +MEE+GFR+ HLYGLTETYGPGTYC W+P WD LP +E+ +
Sbjct: 298 PNKVDVLTGGSPPPPQVLAEMEEMGFRICHLYGLTETYGPGTYCTWKPMWDDLPSDEKSR 357
Query: 541 LKARQGMHHFCLEDIDVKDPETMVNVPADGKTFGEIMFKGNTVMSGYFKDEKATQELLKV 720
++ARQG+HH L+++DV+DP+TM +VP DGKT GEIMF+GNTVMSGYFK++KAT+E
Sbjct: 358 IRARQGIHHVGLQEVDVRDPKTMASVPTDGKTMGEIMFRGNTVMSGYFKNQKATEEAFMG 417
Query: 721 GWFRSGDLTVKHQDGYLEIKDRLKDVIISGDENISTVEVETVLYSHPAILEAAVVARPDN 900
GWF SGD VKH D Y+E+KDRLKDVIISG ENISTVEVET+L+SHP++LEAAVVARPD+
Sbjct: 418 GWFHSGDAAVKHPDHYIEVKDRLKDVIISGGENISTVEVETILFSHPSVLEAAVVARPDD 477
Query: 901 HWGQTPCAFVKLKEGFQ-IDAEGIIKFCRDNLPHYMAPRTVVFEELPRTSTGKVQKFVLR 1077
HWG+TPCAFV LKEG + A+ +I +CRD LPHYMAPRT+VF++LP+TSTGKVQKF+LR
Sbjct: 478 HWGETPCAFVVLKEGCNTVTAQQLIDYCRDRLPHYMAPRTIVFQDLPKTSTGKVQKFILR 537
Query: 1078 EKTKAMGSLS 1107
E+ KAMGSLS
Sbjct: 538 ERAKAMGSLS 547