BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020272
         (1658 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284101.1| PREDICTED: subtilisin-like protease-like iso...   578   0.0  
gb|ABQ58079.1| subtilisin-like protease [Nicotiana tabacum]           581   0.0  
gb|ABQ58080.1| subtilisin-like protease [Nicotiana tabacum]           579   0.0  
ref|XP_002533167.1| Xylem serine proteinase 1 precursor, putativ...   575   0.0  
ref|XP_004167070.1| PREDICTED: subtilisin-like protease-like [Cu...   565   0.0  

>ref|XP_002284101.1| PREDICTED: subtilisin-like protease-like isoform 1 [Vitis vinifera]
          Length = 767

 Score =  578 bits (1490), Expect(2) = 0.0
 Identities = 278/391 (71%), Positives = 325/391 (83%)
 Frame = -1

Query: 1658 LGGGTSDYYRDNIAIGAFAAMEKGILVSCSAGNAGPSAFSLSNVAPWITTVGAGTLDRDF 1479
            LGGG SDYYRD++A+GAF AMEKGILVSCSAGN+GPS +SLSNVAPWITTVGAGTLDRDF
Sbjct: 284  LGGGMSDYYRDSVAMGAFGAMEKGILVSCSAGNSGPSPYSLSNVAPWITTVGAGTLDRDF 343

Query: 1478 PAYASLGNGLNYSGVSLFKGDPLPEKMIPFVYXXXXXXXXXXNLCMPGTLIPEKVKGKLV 1299
            PA+ SLGNG NYSGVSL++GDPLP  ++PFVY          NLCM  TLIPEKV GK+V
Sbjct: 344  PAFVSLGNGKNYSGVSLYRGDPLPGTLLPFVYAGNASNAPNGNLCMTNTLIPEKVAGKMV 403

Query: 1298 LCDRGVNARXXXXXXXXXXXXXXXXXANAASNGDELVADAHLLPATAVTEKTGNLIKSYL 1119
            +CDRGVN R                 AN  +NG+ELVADAHLLPATAV +K+G+ IKSYL
Sbjct: 404  MCDRGVNPRVQKGSVVKAAGGIGMVLANTGTNGEELVADAHLLPATAVGQKSGDAIKSYL 463

Query: 1118 FSEANPTATIVFQGTKVGIEPSPVVAAFSSRGPNSITPEILKPDIIAPGVNILAGWAGSV 939
            FS+ + T TI+F+GTKVGI+PSPVVAAFSSRGPNSITP+ILKPD+IAPGVNILAGW+G+V
Sbjct: 464  FSDHDATVTILFEGTKVGIQPSPVVAAFSSRGPNSITPDILKPDLIAPGVNILAGWSGAV 523

Query: 938  GPTGLEEDSRHVEFNIISGTSMSCPHVSGLAALLKGAHADWSPSAIRSALMTTSYSTYKT 759
            GPTGL  D RHV+FNIISGTSMSCPH+SGLA LLK AH +WSP+AIRSALMTT+Y+ YK+
Sbjct: 524  GPTGLPTDKRHVDFNIISGTSMSCPHISGLAGLLKAAHPEWSPAAIRSALMTTAYTNYKS 583

Query: 758  GKPMQDIATGKPSTPFDHGAGHVDPVSALNPGLVYDLTTEDYVNFLCAIKYTASELKLVT 579
            G+ +QD+ATGKPST FDHGAGHVDPVSALNPGL+YDLT +DY+NFLCAI Y+A ++ ++ 
Sbjct: 584  GQKIQDVATGKPSTAFDHGAGHVDPVSALNPGLIYDLTVDDYLNFLCAINYSAPQISILA 643

Query: 578  RGNFSCDTSKQYSVNDLNYPSFAVSYQTGSG 486
            + NF+CDT K+YSV DLNYPSFAV  QT  G
Sbjct: 644  KRNFTCDTDKKYSVADLNYPSFAVPLQTPLG 674



 Score =  124 bits (312), Expect(2) = 0.0
 Identities = 57/76 (75%), Positives = 67/76 (88%)
 Frame = -2

Query: 391 NVGVPGTYKVSVISPGKSVKVTVEPESLSFSKANEKKSYSVSFTASSMPSNTNLFGRIEW 212
           NVG P TYKVS+ S  +SVK++VEP SLSFS+ NEKKS+ V+FTA+SMPSNTN+FGRIEW
Sbjct: 692 NVGSPSTYKVSIFSESESVKISVEPGSLSFSELNEKKSFKVTFTATSMPSNTNIFGRIEW 751

Query: 211 SDGKHIVGSPIAVSWT 164
           SDGKH+VGSPI VSWT
Sbjct: 752 SDGKHVVGSPIVVSWT 767


>gb|ABQ58079.1| subtilisin-like protease [Nicotiana tabacum]
          Length = 768

 Score =  581 bits (1497), Expect(2) = 0.0
 Identities = 285/386 (73%), Positives = 320/386 (82%)
 Frame = -1

Query: 1658 LGGGTSDYYRDNIAIGAFAAMEKGILVSCSAGNAGPSAFSLSNVAPWITTVGAGTLDRDF 1479
            LGGG SDYYRD++AIGAFAAMEKGILVSCSAGNAGPS +SLSNVAPWITTVGAGTLDRDF
Sbjct: 284  LGGGNSDYYRDSVAIGAFAAMEKGILVSCSAGNAGPSPYSLSNVAPWITTVGAGTLDRDF 343

Query: 1478 PAYASLGNGLNYSGVSLFKGDPLPEKMIPFVYXXXXXXXXXXNLCMPGTLIPEKVKGKLV 1299
            PAY SLGNG N+SGVSL+KGD    KM+PFVY          NLCM GTLIPEKVKGK+V
Sbjct: 344  PAYVSLGNGKNFSGVSLYKGDLSLSKMLPFVYAGNASNTTNGNLCMTGTLIPEKVKGKIV 403

Query: 1298 LCDRGVNARXXXXXXXXXXXXXXXXXANAASNGDELVADAHLLPATAVTEKTGNLIKSYL 1119
            LCDRG+N R                 AN A+NGDELVADAHLLPAT V + TG  IK YL
Sbjct: 404  LCDRGINPRVQKGSVVKEAGGVGMVLANTAANGDELVADAHLLPATTVGQTTGEAIKKYL 463

Query: 1118 FSEANPTATIVFQGTKVGIEPSPVVAAFSSRGPNSITPEILKPDIIAPGVNILAGWAGSV 939
             S+ NPTATI+F+GTKVGI+PSPVVAAFSSRGPNSIT EILKPDIIAPGVNILAGW G+V
Sbjct: 464  TSDPNPTATILFEGTKVGIKPSPVVAAFSSRGPNSITQEILKPDIIAPGVNILAGWTGAV 523

Query: 938  GPTGLEEDSRHVEFNIISGTSMSCPHVSGLAALLKGAHADWSPSAIRSALMTTSYSTYKT 759
            GPTGL ED+R V FNIISGTSMSCPHVSGLAALLKGAH DWSP+AIRSALMTT+Y+ YK 
Sbjct: 524  GPTGLAEDTRRVGFNIISGTSMSCPHVSGLAALLKGAHPDWSPAAIRSALMTTAYTVYKN 583

Query: 758  GKPMQDIATGKPSTPFDHGAGHVDPVSALNPGLVYDLTTEDYVNFLCAIKYTASELKLVT 579
            G  +QD++TGKPSTPFDHGAGHVDPV+ALNPGLVYDL  +DY+NFLCA+ YT+ ++  + 
Sbjct: 584  GGALQDVSTGKPSTPFDHGAGHVDPVAALNPGLVYDLRADDYLNFLCALNYTSIQINSIA 643

Query: 578  RGNFSCDTSKQYSVNDLNYPSFAVSY 501
            R N++C+TSK+YSV DLNYPSFAV +
Sbjct: 644  RRNYNCETSKKYSVTDLNYPSFAVVF 669



 Score =  119 bits (299), Expect(2) = 0.0
 Identities = 56/77 (72%), Positives = 68/77 (88%), Gaps = 1/77 (1%)
 Frame = -2

Query: 391 NVGVPGTYKVS-VISPGKSVKVTVEPESLSFSKANEKKSYSVSFTASSMPSNTNLFGRIE 215
           NVG  GTYKVS V SP  SVKV+VEPE+L F++ANE+KSY+V+FTA SMPS TN++GRIE
Sbjct: 692 NVGPAGTYKVSTVFSPSNSVKVSVEPETLVFTRANEQKSYTVTFTAPSMPSTTNVYGRIE 751

Query: 214 WSDGKHIVGSPIAVSWT 164
           WSDGKH+VGSP+A+SWT
Sbjct: 752 WSDGKHVVGSPVAISWT 768


>gb|ABQ58080.1| subtilisin-like protease [Nicotiana tabacum]
          Length = 768

 Score =  579 bits (1492), Expect(2) = 0.0
 Identities = 284/386 (73%), Positives = 318/386 (82%)
 Frame = -1

Query: 1658 LGGGTSDYYRDNIAIGAFAAMEKGILVSCSAGNAGPSAFSLSNVAPWITTVGAGTLDRDF 1479
            LGGG SDYYRD++AIGAFAAMEKGILVSCSAGNAGP  +SLSNVAPWITTVGAGTLDRDF
Sbjct: 284  LGGGNSDYYRDSVAIGAFAAMEKGILVSCSAGNAGPGPYSLSNVAPWITTVGAGTLDRDF 343

Query: 1478 PAYASLGNGLNYSGVSLFKGDPLPEKMIPFVYXXXXXXXXXXNLCMPGTLIPEKVKGKLV 1299
            PAY SLGNG N+SGVSL+KGD    KM+PFVY          NLCM GTLIPEKVKGK+V
Sbjct: 344  PAYVSLGNGKNFSGVSLYKGDLSLSKMLPFVYAGNASNTTNGNLCMTGTLIPEKVKGKIV 403

Query: 1298 LCDRGVNARXXXXXXXXXXXXXXXXXANAASNGDELVADAHLLPATAVTEKTGNLIKSYL 1119
            LCDRG+N R                 AN A+NGDELVADAHLLPAT V + TG  IK YL
Sbjct: 404  LCDRGINPRVQKGSVVKEAGGVGMVLANTAANGDELVADAHLLPATTVGQTTGEAIKKYL 463

Query: 1118 FSEANPTATIVFQGTKVGIEPSPVVAAFSSRGPNSITPEILKPDIIAPGVNILAGWAGSV 939
             S+ NPTATI+F+GTKVGI+PSPVVAAFSSRGPNSIT EILKPDIIAPGVNILAGW G V
Sbjct: 464  TSDPNPTATILFEGTKVGIKPSPVVAAFSSRGPNSITQEILKPDIIAPGVNILAGWTGGV 523

Query: 938  GPTGLEEDSRHVEFNIISGTSMSCPHVSGLAALLKGAHADWSPSAIRSALMTTSYSTYKT 759
            GPTGL ED+R V FNIISGTSMSCPHVSGLAALLKGAH DWSP+AIRSALMTT+Y+ YK 
Sbjct: 524  GPTGLAEDTRRVGFNIISGTSMSCPHVSGLAALLKGAHPDWSPAAIRSALMTTAYTVYKN 583

Query: 758  GKPMQDIATGKPSTPFDHGAGHVDPVSALNPGLVYDLTTEDYVNFLCAIKYTASELKLVT 579
            G  +QD++TGKPSTPFDHGAGHVDPV+ALNPGLVYDL  +DY+NFLCA+ YT+ ++  + 
Sbjct: 584  GGALQDVSTGKPSTPFDHGAGHVDPVAALNPGLVYDLRADDYLNFLCALNYTSIQINSIA 643

Query: 578  RGNFSCDTSKQYSVNDLNYPSFAVSY 501
            R N++C+TSK+YSV DLNYPSFAV +
Sbjct: 644  RRNYNCETSKKYSVTDLNYPSFAVVF 669



 Score =  112 bits (279), Expect(2) = 0.0
 Identities = 53/76 (69%), Positives = 64/76 (84%), Gaps = 1/76 (1%)
 Frame = -2

Query: 391 NVGVPGTYKVS-VISPGKSVKVTVEPESLSFSKANEKKSYSVSFTASSMPSNTNLFGRIE 215
           NVG  GTYKVS V S   SVKV+VEPE+L F++ NE+KSY+V+FTA S PS TN+FGRIE
Sbjct: 692 NVGPAGTYKVSTVFSSSNSVKVSVEPETLVFTRVNEQKSYTVTFTAPSTPSTTNVFGRIE 751

Query: 214 WSDGKHIVGSPIAVSW 167
           WSDGKH+VGSP+A+SW
Sbjct: 752 WSDGKHVVGSPVAISW 767


>ref|XP_002533167.1| Xylem serine proteinase 1 precursor, putative [Ricinus communis]
            gi|223527039|gb|EEF29226.1| Xylem serine proteinase 1
            precursor, putative [Ricinus communis]
          Length = 760

 Score =  575 bits (1483), Expect(2) = 0.0
 Identities = 279/391 (71%), Positives = 327/391 (83%)
 Frame = -1

Query: 1658 LGGGTSDYYRDNIAIGAFAAMEKGILVSCSAGNAGPSAFSLSNVAPWITTVGAGTLDRDF 1479
            LGGG SDY++D++AIGAFAAMEKGILVSCSAGNAGP+++SLSNVAPWITTVGAGTLDRDF
Sbjct: 283  LGGGMSDYFKDSVAIGAFAAMEKGILVSCSAGNAGPTSYSLSNVAPWITTVGAGTLDRDF 342

Query: 1478 PAYASLGNGLNYSGVSLFKGDPLPEKMIPFVYXXXXXXXXXXNLCMPGTLIPEKVKGKLV 1299
            PA+ SLGNG NYSGVSLFKG  LP K++PF+Y          NLCM  +LIPEKV GK+V
Sbjct: 343  PAFVSLGNGRNYSGVSLFKGSSLPGKLLPFIYAGNASNSTNGNLCMMDSLIPEKVAGKIV 402

Query: 1298 LCDRGVNARXXXXXXXXXXXXXXXXXANAASNGDELVADAHLLPATAVTEKTGNLIKSYL 1119
            LCDRGVNAR                 AN  +NG+ELVADAHLLPAT+V EK GN IKSYL
Sbjct: 403  LCDRGVNARVQKGAVVKEAGGLGMVLANTPANGEELVADAHLLPATSVGEKNGNAIKSYL 462

Query: 1118 FSEANPTATIVFQGTKVGIEPSPVVAAFSSRGPNSITPEILKPDIIAPGVNILAGWAGSV 939
             S+ NPT TI+F+GTKVGI+PSPVVAAFSSRGPNSITP++LKPD+IAPGVNILAGW+G+V
Sbjct: 463  SSDPNPTVTILFEGTKVGIQPSPVVAAFSSRGPNSITPQVLKPDMIAPGVNILAGWSGAV 522

Query: 938  GPTGLEEDSRHVEFNIISGTSMSCPHVSGLAALLKGAHADWSPSAIRSALMTTSYSTYKT 759
            GPTGL  D+R V+FNIISGTSMSCPHVSGLAALLK AH DW+P+AIRSALMTT+Y +YK 
Sbjct: 523  GPTGLSTDTRRVDFNIISGTSMSCPHVSGLAALLKAAHPDWTPAAIRSALMTTAYVSYKN 582

Query: 758  GKPMQDIATGKPSTPFDHGAGHVDPVSALNPGLVYDLTTEDYVNFLCAIKYTASELKLVT 579
            G+ +QD A+GK STPFDHGAGHVDPVSALNPGLVYDLT +DY++FLCA+ YTA+E+  + 
Sbjct: 583  GRNLQDSASGKDSTPFDHGAGHVDPVSALNPGLVYDLTADDYLSFLCALNYTAAEITSLA 642

Query: 578  RGNFSCDTSKQYSVNDLNYPSFAVSYQTGSG 486
            R  F+CD+SK+YS+NDLNYPSFAV++ +  G
Sbjct: 643  RKRFTCDSSKKYSLNDLNYPSFAVNFDSIGG 673



 Score =  115 bits (287), Expect(2) = 0.0
 Identities = 52/75 (69%), Positives = 62/75 (82%)
 Frame = -2

Query: 391 NVGVPGTYKVSVISPGKSVKVTVEPESLSFSKANEKKSYSVSFTASSMPSNTNLFGRIEW 212
           NVG  GTYK S+      VK++VEPE+LSF +ANEKKSY+V+FT SSMP+NTN F R+EW
Sbjct: 685 NVGTAGTYKASISGQALGVKISVEPETLSFIQANEKKSYTVTFTGSSMPTNTNAFARLEW 744

Query: 211 SDGKHIVGSPIAVSW 167
           SDGKH+VGSPIAVSW
Sbjct: 745 SDGKHVVGSPIAVSW 759


>ref|XP_004167070.1| PREDICTED: subtilisin-like protease-like [Cucumis sativus]
          Length = 761

 Score =  565 bits (1457), Expect(2) = 0.0
 Identities = 273/387 (70%), Positives = 320/387 (82%)
 Frame = -1

Query: 1658 LGGGTSDYYRDNIAIGAFAAMEKGILVSCSAGNAGPSAFSLSNVAPWITTVGAGTLDRDF 1479
            LGGG SDYY+D++A GAFAAMEKGILVSCSAGNAGPS FSLSN +PWITTVGAGTLDRDF
Sbjct: 276  LGGGVSDYYKDSVATGAFAAMEKGILVSCSAGNAGPSPFSLSNTSPWITTVGAGTLDRDF 335

Query: 1478 PAYASLGNGLNYSGVSLFKGDPLPEKMIPFVYXXXXXXXXXXNLCMPGTLIPEKVKGKLV 1299
            PAY SLG+  N+SGVSL++G  LP  ++PF+Y          NLCM GTLIPEKV GK+V
Sbjct: 336  PAYVSLGDAKNFSGVSLYRGKSLPGTLLPFIYAANASNSGNGNLCMTGTLIPEKVAGKVV 395

Query: 1298 LCDRGVNARXXXXXXXXXXXXXXXXXANAASNGDELVADAHLLPATAVTEKTGNLIKSYL 1119
             CDRGVN R                 AN A+NG+ELVAD+HLLPATAV +K+G+ I+ YL
Sbjct: 396  FCDRGVNPRVQKGAVVKAAGGIGMVLANTAANGEELVADSHLLPATAVGQKSGDTIRKYL 455

Query: 1118 FSEANPTATIVFQGTKVGIEPSPVVAAFSSRGPNSITPEILKPDIIAPGVNILAGWAGSV 939
             S+ +PT TI+F+GTK+GIEPSPVVAAFSSRGPNSITP++LKPDIIAPGVNILAGW+ SV
Sbjct: 456  VSDPSPTVTILFEGTKLGIEPSPVVAAFSSRGPNSITPQLLKPDIIAPGVNILAGWSKSV 515

Query: 938  GPTGLEEDSRHVEFNIISGTSMSCPHVSGLAALLKGAHADWSPSAIRSALMTTSYSTYKT 759
            GP+GL  D R V+FNIISGTSMSCPHVSGLAAL+KGAH DWSP+AIRSALMTT+Y+ YK 
Sbjct: 516  GPSGLAIDDRRVDFNIISGTSMSCPHVSGLAALIKGAHPDWSPAAIRSALMTTAYTAYKN 575

Query: 758  GKPMQDIATGKPSTPFDHGAGHVDPVSALNPGLVYDLTTEDYVNFLCAIKYTASELKLVT 579
            G+ +QDIATGKPSTPFDHGAGHVDPVSALNPGLVYDLT +DY+NFLCA+ YT S++  + 
Sbjct: 576  GQKIQDIATGKPSTPFDHGAGHVDPVSALNPGLVYDLTVDDYLNFLCALNYTPSQINSLA 635

Query: 578  RGNFSCDTSKQYSVNDLNYPSFAVSYQ 498
            R +F+CD+ K+YSVNDLNYPSFAV ++
Sbjct: 636  RKDFTCDSKKKYSVNDLNYPSFAVVFE 662



 Score =  112 bits (281), Expect(2) = 0.0
 Identities = 54/79 (68%), Positives = 66/79 (83%), Gaps = 3/79 (3%)
 Frame = -2

Query: 391 NVGVPGTYKVSVISPGKSVKVTVEPESLSFSKANEKKSYSVSF---TASSMPSNTNLFGR 221
           NVG PGTYKVS+ S  KSVK++VEPESLSF+ AN+KKSY+V+F   T+S+ P++   FGR
Sbjct: 683 NVGSPGTYKVSITSETKSVKISVEPESLSFTGANDKKSYTVTFTTTTSSAAPTSAEAFGR 742

Query: 220 IEWSDGKHIVGSPIAVSWT 164
           IEWSDGKH+VGSPIA SWT
Sbjct: 743 IEWSDGKHVVGSPIAFSWT 761