BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020179
         (1047 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002284945.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...   556   e-156
ref|XP_004147267.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...   525   e-147
ref|XP_003538048.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...   521   e-145
ref|XP_003539732.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like ...   519   e-145
ref|XP_002526365.1| sumo ligase, putative [Ricinus communis] gi|...   518   e-144

>ref|XP_002284945.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like [Vitis vinifera]
          Length = 876

 Score =  556 bits (1432), Expect = e-156
 Identities = 270/341 (79%), Positives = 306/341 (89%)
 Frame = -1

Query: 1026 MDLAASCKEQLAYFRIKELKDVLTQLGLSKQGKKQDLVERLLTILSDDRVSGMWAKKNAV 847
            MDL  SCK++LAYFRIKELKDVLTQLGLSKQGKKQDLV+R+L ILSD++VS MWAKKNAV
Sbjct: 1    MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAILSDEQVSRMWAKKNAV 60

Query: 846  GKEEVAKLIDDTYRKMKASGAATDLASKGQGLSDSSNAKSKEEPRLEDSYQMEKIRCVCG 667
            GKEEVAKL++DTYRKM+ SGA TDLASKGQ LSDSSN K KEE  LEDSY   KIRC CG
Sbjct: 61   GKEEVAKLVEDTYRKMQVSGA-TDLASKGQVLSDSSNVKFKEE--LEDSYNDMKIRCPCG 117

Query: 666  SSLKADSMIKCEDSRCNVWQHIGCVLIPEKVLEGVIPAPPDVFYCELCRLNRADPFWVTM 487
            S+L  ++M+KC+D +C VWQHIGCV+IPEK +EG IP  PD FYCE+CRL+RADPFWVT+
Sbjct: 118  SALPNETMLKCDDLKCQVWQHIGCVIIPEKTMEG-IPPTPDPFYCEICRLSRADPFWVTV 176

Query: 486  AHPLYPVKLSIANRPTDGTNPVQSIEKTFQLTRADKDLLAKPEYDVQAWCILLNDKVSFR 307
            AHPL PVKL+  + PTDGTNPVQS+EKTF LTRAD+D+++K EYDVQAWCILLNDKVSFR
Sbjct: 177  AHPLLPVKLTTTSIPTDGTNPVQSVEKTFHLTRADRDMVSKHEYDVQAWCILLNDKVSFR 236

Query: 306  MQWPQYADIQVNGVPVRAINRPGSQLLGANGRDDGPVITLCTRDGMNKISVTGCDTRVFC 127
            MQWPQYAD+QVNG+ VRAINRPGSQLLGANGRDDGPVIT CT+DG+NKIS+TGCD R+FC
Sbjct: 237  MQWPQYADLQVNGMAVRAINRPGSQLLGANGRDDGPVITPCTKDGINKISLTGCDARIFC 296

Query: 126  VGVRIVKRRTVQQILNLIPKESDGEMLEDALARVRRCVGGG 4
            +GVRIVKRRTVQQIL+LIPKESDGE  EDALARVRRC+GGG
Sbjct: 297  LGVRIVKRRTVQQILSLIPKESDGERFEDALARVRRCIGGG 337


>ref|XP_004147267.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like [Cucumis sativus]
            gi|449511458|ref|XP_004163961.1| PREDICTED: E3
            SUMO-protein ligase SIZ1-like [Cucumis sativus]
          Length = 869

 Score =  525 bits (1353), Expect = e-147
 Identities = 252/345 (73%), Positives = 302/345 (87%), Gaps = 4/345 (1%)
 Frame = -1

Query: 1026 MDLAASCKEQLAYFRIKELKDVLTQLGLSKQGKKQDLVERLLTILSDDRVSGMWAKKNAV 847
            MDL A+CK++LAYFRIKELKD+LTQLGLSKQGKKQDLV+R+L ILSD++VS MWAKKNAV
Sbjct: 1    MDLVANCKDKLAYFRIKELKDILTQLGLSKQGKKQDLVQRILDILSDEQVSKMWAKKNAV 60

Query: 846  GKEEVAKLIDDTYRKMKASGAATDLASKGQGLSDSSNAKSKEEPRLEDSYQME-KIRCVC 670
            GK++VAKL+DDTYRKM+ SG   DLA+KGQG+SDSSN + K E   +DS Q++ K+RC+C
Sbjct: 61   GKDQVAKLVDDTYRKMQVSGV--DLATKGQGVSDSSNVQVKGET--DDSLQLDTKVRCLC 116

Query: 669  GSSLKADSMIKCEDSRCNVWQHIGCVLIPEKVLEGVIPAPPDVFYCELCRLNRADPFWVT 490
            G+ L+ +SMIKCED RC VWQHI CV++PEK  EG  P  P+ FYCE+CRLNRADPFWV+
Sbjct: 117  GNGLQTESMIKCEDPRCQVWQHISCVIVPEKPTEGN-PPYPEHFYCEICRLNRADPFWVS 175

Query: 489  MAHPLYPVKLSIA---NRPTDGTNPVQSIEKTFQLTRADKDLLAKPEYDVQAWCILLNDK 319
            +AHPL+PVKL      N PTDGTNP+QS++++FQLTRADKDLL+K EYDVQAWC+LLNDK
Sbjct: 176  VAHPLFPVKLITTMSTNIPTDGTNPMQSVDRSFQLTRADKDLLSKQEYDVQAWCMLLNDK 235

Query: 318  VSFRMQWPQYADIQVNGVPVRAINRPGSQLLGANGRDDGPVITLCTRDGMNKISVTGCDT 139
            V FRMQWPQYAD+Q+NG+ VRAINRPGSQLLGANGRDDGP+IT CT+DGMNKI++TGCD 
Sbjct: 236  VPFRMQWPQYADLQINGLAVRAINRPGSQLLGANGRDDGPIITACTKDGMNKIALTGCDA 295

Query: 138  RVFCVGVRIVKRRTVQQILNLIPKESDGEMLEDALARVRRCVGGG 4
            R FC+GVRIVKRRTVQQIL++IPKESDGE  +DALAR+ RC+GGG
Sbjct: 296  RSFCLGVRIVKRRTVQQILSMIPKESDGERFQDALARICRCIGGG 340


>ref|XP_003538048.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like [Glycine max]
          Length = 879

 Score =  521 bits (1341), Expect = e-145
 Identities = 252/342 (73%), Positives = 297/342 (86%), Gaps = 1/342 (0%)
 Frame = -1

Query: 1026 MDLAASCKEQLAYFRIKELKDVLTQLGLSKQGKKQDLVERLLTILSDDRVSGMWAKKNAV 847
            MDL  S KE+L YFRIKELKDVLTQL LSKQGKKQDLV+R+L++LSD++VS MWAKKNA 
Sbjct: 1    MDLVPSVKEKLNYFRIKELKDVLTQLHLSKQGKKQDLVDRILSVLSDEQVSKMWAKKNAG 60

Query: 846  GKEEVAKLIDDTYRKMKASGAATDLASKGQGLSDSSNAKSKEEPRLEDSYQME-KIRCVC 670
            GKE+VAKL+DDTYRKM+ SGA TDLASKGQG SDSS+ K K E   +D++Q + KIRC+C
Sbjct: 61   GKEQVAKLVDDTYRKMQISGA-TDLASKGQGASDSSSVKVKSE--FDDAFQRDVKIRCLC 117

Query: 669  GSSLKADSMIKCEDSRCNVWQHIGCVLIPEKVLEGVIPAPPDVFYCELCRLNRADPFWVT 490
            GS L+ + ++KC+D RC+VWQHI CV+IPEK  EG IP  PD FYCELCRL RADPFWV+
Sbjct: 118  GSRLETEDLVKCDDPRCHVWQHISCVIIPEKPTEG-IPPVPDKFYCELCRLTRADPFWVS 176

Query: 489  MAHPLYPVKLSIANRPTDGTNPVQSIEKTFQLTRADKDLLAKPEYDVQAWCILLNDKVSF 310
            +AHPL+PVKL+  + PTDG NPVQS+E+TFQLTRAD DL++KPE+DV+AWC+LLNDKV F
Sbjct: 177  VAHPLHPVKLTTTSNPTDGNNPVQSVERTFQLTRADMDLVSKPEFDVEAWCMLLNDKVPF 236

Query: 309  RMQWPQYADIQVNGVPVRAINRPGSQLLGANGRDDGPVITLCTRDGMNKISVTGCDTRVF 130
            RMQWPQY D+QVNGVPVRA NRPGSQLLGANGRDDGP+IT  T+DG+NKIS+TGCD R+F
Sbjct: 237  RMQWPQYTDLQVNGVPVRATNRPGSQLLGANGRDDGPIITPYTKDGINKISLTGCDARIF 296

Query: 129  CVGVRIVKRRTVQQILNLIPKESDGEMLEDALARVRRCVGGG 4
            C+GVRIVKRR++QQILN IPKESDGE  E+ALARV RCVGGG
Sbjct: 297  CLGVRIVKRRSMQQILNSIPKESDGEKFEEALARVCRCVGGG 338


>ref|XP_003539732.1| PREDICTED: E3 SUMO-protein ligase SIZ1-like [Glycine max]
          Length = 880

 Score =  519 bits (1337), Expect = e-145
 Identities = 253/343 (73%), Positives = 300/343 (87%), Gaps = 2/343 (0%)
 Frame = -1

Query: 1026 MDLAASCKEQLAYFRIKELKDVLTQLGLSKQGKKQDLVERLLTILSDDRVSGMWAKKNAV 847
            MDL  S KE+L YFRIKELKDVLTQL LSKQGKKQDLV+R+L++LSD++VS +WAKKNA 
Sbjct: 1    MDLVPSVKEKLNYFRIKELKDVLTQLHLSKQGKKQDLVDRILSVLSDEQVSKIWAKKNAG 60

Query: 846  G-KEEVAKLIDDTYRKMKASGAATDLASKGQGLSDSSNAKSKEEPRLEDSYQME-KIRCV 673
            G KE+VAKL+DDTYRKM+ SGA TDLASKGQG SDSS+ K K E   +D++Q + KIRC+
Sbjct: 61   GGKEQVAKLVDDTYRKMQISGA-TDLASKGQGASDSSSVKVKSE--FDDAFQPDVKIRCL 117

Query: 672  CGSSLKADSMIKCEDSRCNVWQHIGCVLIPEKVLEGVIPAPPDVFYCELCRLNRADPFWV 493
            CGS L+ ++++KC+D+RC+VWQHI CV+IPEK  EG IP  PD FYCELCRL RADPFWV
Sbjct: 118  CGSRLETENLVKCDDARCHVWQHISCVIIPEKPTEG-IPLVPDKFYCELCRLTRADPFWV 176

Query: 492  TMAHPLYPVKLSIANRPTDGTNPVQSIEKTFQLTRADKDLLAKPEYDVQAWCILLNDKVS 313
            ++AHPL+PVKL+  + PTDG NPVQS+E+TFQLTRADKDL++KPE+DV+AWC+LLNDKV 
Sbjct: 177  SVAHPLHPVKLTTTSNPTDGNNPVQSVERTFQLTRADKDLVSKPEFDVEAWCMLLNDKVP 236

Query: 312  FRMQWPQYADIQVNGVPVRAINRPGSQLLGANGRDDGPVITLCTRDGMNKISVTGCDTRV 133
            FRMQWPQY D+QVNGVPVRA NRPGSQLLGANGRDDGP+IT  T+DG+NKIS+TGCD R+
Sbjct: 237  FRMQWPQYTDLQVNGVPVRATNRPGSQLLGANGRDDGPIITPYTKDGINKISLTGCDARI 296

Query: 132  FCVGVRIVKRRTVQQILNLIPKESDGEMLEDALARVRRCVGGG 4
            FC+GVRIVKRR++QQILN IPKESDGE  EDALARV RCVGGG
Sbjct: 297  FCLGVRIVKRRSMQQILNSIPKESDGEKFEDALARVCRCVGGG 339


>ref|XP_002526365.1| sumo ligase, putative [Ricinus communis] gi|223534324|gb|EEF36036.1|
            sumo ligase, putative [Ricinus communis]
          Length = 876

 Score =  518 bits (1333), Expect = e-144
 Identities = 256/343 (74%), Positives = 294/343 (85%), Gaps = 1/343 (0%)
 Frame = -1

Query: 1026 MDLAASCKEQLAYFRIKELKDVLTQLGLSKQGKKQDLVERLLTILSDDRVSGMWAKKNAV 847
            MDL  SCK++LAYFRIKELKDVLTQLGLSKQGKKQDLV+R+L +L+D++V    AKK+ V
Sbjct: 1    MDLVTSCKDKLAYFRIKELKDVLTQLGLSKQGKKQDLVDRILAVLTDEQVPKTSAKKSVV 60

Query: 846  GKEEVAKLIDDTYRKMKASGAATDLASKGQGLSDSSNAKSKEEPRLEDSYQME-KIRCVC 670
            GKEEVAKL+DD YRKM+ SGA TDLASKG+G+ +SS    K E  ++DS+  + K+RC C
Sbjct: 61   GKEEVAKLVDDIYRKMQVSGA-TDLASKGEGVLESSKPVIKGE--IDDSFHFDTKVRCPC 117

Query: 669  GSSLKADSMIKCEDSRCNVWQHIGCVLIPEKVLEGVIPAPPDVFYCELCRLNRADPFWVT 490
            GSSL+ +SMIKCED RC VWQHIGCV+IPEK +E  IP  PD+FYCE+CRL RADPFWV+
Sbjct: 118  GSSLETESMIKCEDPRCRVWQHIGCVIIPEKPMEA-IPQVPDLFYCEICRLCRADPFWVS 176

Query: 489  MAHPLYPVKLSIANRPTDGTNPVQSIEKTFQLTRADKDLLAKPEYDVQAWCILLNDKVSF 310
            +AHPLYPVKL+  N   DG+ PVQS EKTF LTRADKDLLAK EYDVQAWC+LLNDKV F
Sbjct: 177  VAHPLYPVKLT-TNIQADGSTPVQSAEKTFHLTRADKDLLAKQEYDVQAWCMLLNDKVPF 235

Query: 309  RMQWPQYADIQVNGVPVRAINRPGSQLLGANGRDDGPVITLCTRDGMNKISVTGCDTRVF 130
            RMQWPQYAD+QVNGVPVRAINRPGSQLLG NGRDDGP+IT CT+DG+NKIS+ GCD R+F
Sbjct: 236  RMQWPQYADLQVNGVPVRAINRPGSQLLGINGRDDGPIITPCTKDGINKISLNGCDARIF 295

Query: 129  CVGVRIVKRRTVQQILNLIPKESDGEMLEDALARVRRCVGGGA 1
            C+GVRIVKRRTVQQILN+IPKESDGE  EDALARV RCVGGGA
Sbjct: 296  CLGVRIVKRRTVQQILNMIPKESDGERFEDALARVCRCVGGGA 338