BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020160
         (1425 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272750.1| PREDICTED: multisubstrate pseudouridine synt...   663   0.0  
emb|CBI34194.3| unnamed protein product [Vitis vinifera]              650   0.0  
ref|XP_002512230.1| tRNA pseudouridine synthase d, putative [Ric...   648   0.0  
ref|XP_003520835.1| PREDICTED: multisubstrate pseudouridine synt...   632   e-179
ref|NP_187133.3| pseudouridine synthase [Arabidopsis thaliana] g...   630   e-178

>ref|XP_002272750.1| PREDICTED: multisubstrate pseudouridine synthase 7-like [Vitis
            vinifera]
          Length = 694

 Score =  663 bits (1711), Expect = 0.0
 Identities = 325/425 (76%), Positives = 373/425 (87%), Gaps = 1/425 (0%)
 Frame = +1

Query: 154  MNSVDESDVGITCFISQLPGFRGILKQRYADFIVNEVDLDGNVIHLTSLVAPPEIAEEKV 333
            M  ++ESDVGI+ +ISQLPGFRGILKQRY DFIVNEVD DGNV+HLTSL APPE  E+  
Sbjct: 1    MKHLEESDVGISFYISQLPGFRGILKQRYCDFIVNEVDSDGNVVHLTSLDAPPESMEKNE 60

Query: 334  VKVTDEPNKSYATEIESFRALAGDADANSLKSFIDDINSGSED-VAHITLSPSSDKSHRT 510
             K++D+ NK YA+EIE FR+L GD+D   LK  ID + SG ED V+ I LS SSDKSHRT
Sbjct: 61   TKISDQSNKGYASEIELFRSLVGDSDVECLKGLIDQVASGIEDGVSPIVLSSSSDKSHRT 120

Query: 511  AMHNFFKENLKFLVTDTVDGPEASSKCIRVRLNSGGNTGRGKNSKKRKERNDKSYDSRGS 690
            A+HNFFKENLKFLVTDT+DGP+ SSKCIRVRLNSGG+   G+NS+KRK+R DK +DSRGS
Sbjct: 121  AVHNFFKENLKFLVTDTIDGPDTSSKCIRVRLNSGGSHNNGRNSRKRKDRRDKPFDSRGS 180

Query: 691  KSWPENLGKFIKFHLYKENKDTQEALGVIGKMLGIQPRSFGFAGTKDKRAISTQRVTLYK 870
             +W E++GKF++FHLYKENKDTQEA+G+IGKMLGIQPRSFGFAGTKDKR++STQRVT++K
Sbjct: 181  DNWSEHVGKFLRFHLYKENKDTQEAIGLIGKMLGIQPRSFGFAGTKDKRSVSTQRVTVFK 240

Query: 871  VRASRLAALNSRLIGIKIGDFCHVDEGLLLGQLYGNKFTITLRGVTADSDNTIKASADAL 1050
              A+RLAALN RLIGIK+GDFCHV EGL+LGQL GN+FTITLRGV ADS+ TIKASADAL
Sbjct: 241  QHANRLAALNERLIGIKVGDFCHVREGLVLGQLLGNRFTITLRGVVADSEETIKASADAL 300

Query: 1051 GRHGFINYFGLQRFGSGSLPTHMIGAALFRGEWKSAVDMILDPREGERENIRQVREYYKE 1230
            GR GFINYFGLQRFGSGS+PTH+IGAAL RGEW++AV +ILDPREGER+ IR+ REYYKE
Sbjct: 301  GRLGFINYFGLQRFGSGSVPTHLIGAALLRGEWETAVSLILDPREGERDAIRKAREYYKE 360

Query: 1231 SDDIEGTLRQLPRYMVAERAILQGLKKCPGNYLLALKAIPRTLRMMYVHSYQSFLWNHAA 1410
            SDDIEGTLRQ PR++VAERAILQ LKK PGNYL ALKAIPRTLRMMYVHSYQS+LWNHAA
Sbjct: 361  SDDIEGTLRQFPRHLVAERAILQCLKKYPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAA 420

Query: 1411 STRVQ 1425
            S RVQ
Sbjct: 421  SMRVQ 425


>emb|CBI34194.3| unnamed protein product [Vitis vinifera]
          Length = 693

 Score =  650 bits (1676), Expect = 0.0
 Identities = 321/425 (75%), Positives = 370/425 (87%), Gaps = 1/425 (0%)
 Frame = +1

Query: 154  MNSVDESDVGITCFISQLPGFRGILKQRYADFIVNEVDLDGNVIHLTSLVAPPEIAEEKV 333
            M  ++ESDVGI+ +ISQLPGFRGILKQRY DFIVNEVD DGNV+HLTSL APPE  E+  
Sbjct: 1    MKHLEESDVGISFYISQLPGFRGILKQRYCDFIVNEVDSDGNVVHLTSLDAPPESMEKNE 60

Query: 334  VKVTDEPNKSYATEIESFRALAGDADANSLKSFIDDINSGSED-VAHITLSPSSDKSHRT 510
             K++D+ NK YA+EIE FR+L GD+D   LK  ID + SG ED V+ I LS SSDKSHRT
Sbjct: 61   TKISDQSNKGYASEIELFRSLVGDSDVECLKGLIDQVASGIEDGVSPIVLSSSSDKSHRT 120

Query: 511  AMHNFFKENLKFLVTDTVDGPEASSKCIRVRLNSGGNTGRGKNSKKRKERNDKSYDSRGS 690
            A+HNFFKENLKFLVTDT+DGP+ SSKCIRVRLNSGG+   G+NS+KRK+R DK +DSRGS
Sbjct: 121  AVHNFFKENLKFLVTDTIDGPDTSSKCIRVRLNSGGSHNNGRNSRKRKDRRDKPFDSRGS 180

Query: 691  KSWPENLGKFIKFHLYKENKDTQEALGVIGKMLGIQPRSFGFAGTKDKRAISTQRVTLYK 870
             +W E++GKF++FHLYKENKDTQEA+G+IGKMLGIQPRSFGFAGTKDKR++STQRVT++K
Sbjct: 181  DNWSEHVGKFLRFHLYKENKDTQEAIGLIGKMLGIQPRSFGFAGTKDKRSVSTQRVTVFK 240

Query: 871  VRASRLAALNSRLIGIKIGDFCHVDEGLLLGQLYGNKFTITLRGVTADSDNTIKASADAL 1050
              A+RLAALN RLIGIK+GDFCHV EGL+LGQL GN+FTITLRGV ADS+ TIKASADAL
Sbjct: 241  QHANRLAALNERLIGIKVGDFCHVREGLVLGQLLGNRFTITLRGVVADSEETIKASADAL 300

Query: 1051 GRHGFINYFGLQRFGSGSLPTHMIGAALFRGEWKSAVDMILDPREGERENIRQVREYYKE 1230
            GR GFINYFGLQRFGSGS+PTH+IGAAL RGEW++AV +ILDPREG+   + + REYYKE
Sbjct: 301  GRLGFINYFGLQRFGSGSVPTHLIGAALLRGEWETAVSLILDPREGD-ILLLKAREYYKE 359

Query: 1231 SDDIEGTLRQLPRYMVAERAILQGLKKCPGNYLLALKAIPRTLRMMYVHSYQSFLWNHAA 1410
            SDDIEGTLRQ PR++VAERAILQ LKK PGNYL ALKAIPRTLRMMYVHSYQS+LWNHAA
Sbjct: 360  SDDIEGTLRQFPRHLVAERAILQCLKKYPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAA 419

Query: 1411 STRVQ 1425
            S RVQ
Sbjct: 420  SMRVQ 424


>ref|XP_002512230.1| tRNA pseudouridine synthase d, putative [Ricinus communis]
            gi|223548191|gb|EEF49682.1| tRNA pseudouridine synthase
            d, putative [Ricinus communis]
          Length = 753

 Score =  648 bits (1672), Expect = 0.0
 Identities = 331/482 (68%), Positives = 394/482 (81%), Gaps = 14/482 (2%)
 Frame = +1

Query: 22   KFVAKLKPYNRQHNWALKPYKQSLNLNRSATSFCRINVEFSPET-MNSVDESDVGITCFI 198
            +++A LKPY          +  S + +   T+   +  + S +T M +++ESDVGI+C+I
Sbjct: 10   RYLAVLKPYYPPS------FSSSCSNSTYRTTTATLVADHSFKTLMTTIEESDVGISCYI 63

Query: 199  SQLPGFRGILKQRYADFIVNEVDLDGNVIHLTSLVAPPEI---AEEKVVKVTDEPNKSYA 369
            S++PGFRGILKQRY+DFIVNEVD +GNV+HLTSL  P EI   AEE+  ++T++  K+YA
Sbjct: 64   SKVPGFRGILKQRYSDFIVNEVDNNGNVVHLTSLHVPREIVEVAEEREEEITNQVGKNYA 123

Query: 370  TEIESFRALAGDADANSLKSFIDDINSGSE--DVAHITLSPSSDKSHRTAMHNFFKENLK 543
             EIESFR LAGDAD   L++FI+ + S     +++ I LSP SDK+HRTA HNFFKE LK
Sbjct: 124  AEIESFRELAGDADTQRLEAFINQVISEDNCINISPILLSPDSDKAHRTATHNFFKEKLK 183

Query: 544  FLVTDTVDGPEASSKCIRVRLNSGGNTGRG-KNSKKRKERNDKSYDSRGSKSWPENLGKF 720
            FLVTDTVDGP+ASSKCIRVRLNS GN   G +NSKKRK+RNDK +DSRGS  WPE+LGKF
Sbjct: 184  FLVTDTVDGPDASSKCIRVRLNSRGNINEGNRNSKKRKDRNDKPFDSRGSDHWPEHLGKF 243

Query: 721  IKFHLYKENKDTQEALGVIGKMLGIQPRSFGFAGTKDKRAISTQRVTLYKVRASRLAALN 900
            ++FHLYKENKDTQEA+G+IGKMLGIQPRSFGFAGTKDKR++STQRVT++K  ASRLAALN
Sbjct: 244  LRFHLYKENKDTQEAIGLIGKMLGIQPRSFGFAGTKDKRSVSTQRVTVFKQHASRLAALN 303

Query: 901  SRLIGIKIGDFCHVDEGLLLGQLYGNKFTITLRGVTADSDNTIKASADALGRHGFINYFG 1080
             RLIGIK+GDFC+V+EGLLLGQL GN+FT+TLRGV ADS++TIK SAD+LGRHGFINYFG
Sbjct: 304  DRLIGIKVGDFCYVNEGLLLGQLLGNRFTVTLRGVVADSEDTIKGSADSLGRHGFINYFG 363

Query: 1081 LQRFGSGSLPTHMIGAALFRGEWKSAVDMILDPREGERENI-------RQVREYYKESDD 1239
            LQRFGSGS+PTH+IGAAL RGEWK AV MILDPREG+   +       R  REYYKESDD
Sbjct: 364  LQRFGSGSVPTHLIGAALLRGEWKYAVSMILDPREGDILLLVICFMWMRMAREYYKESDD 423

Query: 1240 IEGTLRQLPRYMVAERAILQGLKKCPGNYLLALKAIPRTLRMMYVHSYQSFLWNHAASTR 1419
            I+GTLRQLPRY+ AERA+LQ LKKCPGNYL ALKAIPRTLRMMYVHSYQS+LWNHAAS R
Sbjct: 424  IDGTLRQLPRYLTAERAVLQCLKKCPGNYLQALKAIPRTLRMMYVHSYQSYLWNHAASMR 483

Query: 1420 VQ 1425
            VQ
Sbjct: 484  VQ 485


>ref|XP_003520835.1| PREDICTED: multisubstrate pseudouridine synthase 7-like [Glycine max]
          Length = 687

 Score =  632 bits (1629), Expect = e-179
 Identities = 311/425 (73%), Positives = 362/425 (85%), Gaps = 1/425 (0%)
 Frame = +1

Query: 154  MNSVDESDVGITCFISQLPGFRGILKQRYADFIVNEVDLDGNVIHLTSLVAPPEIAEEKV 333
            M + +E +VGI+CFIS LPGFRGILKQRY+DFIVNEVD DG V+ L+SL AP E  EE  
Sbjct: 3    MKTAEEHEVGISCFISDLPGFRGILKQRYSDFIVNEVDRDGTVVQLSSLDAPQEEPEENG 62

Query: 334  VKVTDEPNKSYATEIESFRALAGDADANSLKSFIDDINSGSED-VAHITLSPSSDKSHRT 510
               +D    SYA++IESF++LAGD+DA  L+ FI+ IN+G ED V+ I LSP SDKSHR 
Sbjct: 63   TNTSDNV-VSYASQIESFKSLAGDSDAVLLEEFINKINAGGEDGVSPIVLSPDSDKSHRK 121

Query: 511  AMHNFFKENLKFLVTDTVDGPEASSKCIRVRLNSGGNTGRGKNSKKRKERNDKSYDSRGS 690
            AMHNFFKEN KFLVTD VDGP+ASSKCIRVRLNS     +GKNSKKRKER DK +DSRGS
Sbjct: 122  AMHNFFKENFKFLVTDVVDGPDASSKCIRVRLNSAEPNNKGKNSKKRKERGDKPFDSRGS 181

Query: 691  KSWPENLGKFIKFHLYKENKDTQEALGVIGKMLGIQPRSFGFAGTKDKRAISTQRVTLYK 870
            ++WP+N+GKF++FHLYKENKDT EALGVIG ML +QP+SFGFAGTKDKRA++TQRVT+YK
Sbjct: 182  ENWPKNVGKFLRFHLYKENKDTHEALGVIGNMLSVQPKSFGFAGTKDKRAVTTQRVTVYK 241

Query: 871  VRASRLAALNSRLIGIKIGDFCHVDEGLLLGQLYGNKFTITLRGVTADSDNTIKASADAL 1050
             RASRLA+LN RL GIK+GDFC+V EGL LGQL GN+FTITLRG+ ADS++ IKASADAL
Sbjct: 242  QRASRLASLNKRLFGIKLGDFCYVKEGLCLGQLLGNRFTITLRGIVADSEDIIKASADAL 301

Query: 1051 GRHGFINYFGLQRFGSGSLPTHMIGAALFRGEWKSAVDMILDPREGERENIRQVREYYKE 1230
            GRHGFINYFGLQRFGSGSLPTH+IGAAL +GEWK AVDMILDPR+GE+  I + R+YYKE
Sbjct: 302  GRHGFINYFGLQRFGSGSLPTHLIGAALLQGEWKLAVDMILDPRDGEKSAIAKARKYYKE 361

Query: 1231 SDDIEGTLRQLPRYMVAERAILQGLKKCPGNYLLALKAIPRTLRMMYVHSYQSFLWNHAA 1410
            S D+ GTL+QLPRY+VAERA+LQ LK  PGNYL ALK+IPRTLRM+YVHSYQS+LWNHAA
Sbjct: 362  SSDVVGTLKQLPRYLVAERAVLQTLKTSPGNYLQALKSIPRTLRMLYVHSYQSYLWNHAA 421

Query: 1411 STRVQ 1425
            S RVQ
Sbjct: 422  SKRVQ 426


>ref|NP_187133.3| pseudouridine synthase [Arabidopsis thaliana]
            gi|52354299|gb|AAU44470.1| hypothetical protein AT3G04820
            [Arabidopsis thaliana] gi|61742699|gb|AAX55170.1|
            hypothetical protein At3g04820 [Arabidopsis thaliana]
            gi|332640619|gb|AEE74140.1| pseudouridine synthase
            [Arabidopsis thaliana]
          Length = 715

 Score =  630 bits (1626), Expect = e-178
 Identities = 312/455 (68%), Positives = 374/455 (82%), Gaps = 3/455 (0%)
 Frame = +1

Query: 70   LKPYKQSLNLNRSATSFCRINVEFSPETMNSVDESDVGITCFISQLPGFRGILKQRYADF 249
            LKPY       + AT      +  + E M +VDE+DVGI+CFISQLPGFRGILKQRYADF
Sbjct: 15   LKPYSSLSWTTKIAT------IATNFEGMKTVDEADVGISCFISQLPGFRGILKQRYADF 68

Query: 250  IVNEVDLDGNVIHLTSLVAPPEIA--EEKVVKVTDEPNKSYATEIESFRALAGDADANSL 423
            IVNEVD+DGNV+HLTSL  PPE+A  EE   K  D   ++Y + +ESF++++   DA  L
Sbjct: 69   IVNEVDMDGNVVHLTSLDLPPEMAVKEEDKAKSCDNVPQNYTSAVESFKSISSVTDAERL 128

Query: 424  KSFIDDINSGSE-DVAHITLSPSSDKSHRTAMHNFFKENLKFLVTDTVDGPEASSKCIRV 600
            +  I+ + SG++ +V  + L PSSDKS RTA+HNF KEN KFLVTDTVDGP+++SKCIRV
Sbjct: 129  EILINQVTSGTDGEVLPVVLDPSSDKSKRTAIHNFVKENFKFLVTDTVDGPDSTSKCIRV 188

Query: 601  RLNSGGNTGRGKNSKKRKERNDKSYDSRGSKSWPENLGKFIKFHLYKENKDTQEALGVIG 780
            R+NSG N  RG  SKKRKER DK +DSRGS+ WPE++GKF++FHLYKENKDTQEALG+IG
Sbjct: 189  RVNSGANNNRGNRSKKRKERGDKPFDSRGSEHWPEHVGKFLRFHLYKENKDTQEALGLIG 248

Query: 781  KMLGIQPRSFGFAGTKDKRAISTQRVTLYKVRASRLAALNSRLIGIKIGDFCHVDEGLLL 960
            KMLG+QP+SFGF+GTKDKR++STQRVT++K +AS+LAALN RL GIK+GDFC+V EGLLL
Sbjct: 249  KMLGVQPKSFGFSGTKDKRSVSTQRVTVFKQQASKLAALNKRLFGIKVGDFCNVKEGLLL 308

Query: 961  GQLYGNKFTITLRGVTADSDNTIKASADALGRHGFINYFGLQRFGSGSLPTHMIGAALFR 1140
            GQL GN+FTITLRGV ADS+ TIK SA++LG+ GFINYFGLQRFGSGS+PTH +GAAL +
Sbjct: 309  GQLMGNRFTITLRGVVADSEETIKQSAESLGKDGFINYFGLQRFGSGSVPTHHVGAALLK 368

Query: 1141 GEWKSAVDMILDPREGERENIRQVREYYKESDDIEGTLRQLPRYMVAERAILQGLKKCPG 1320
            GEWK AVDMILDPRE ER  +   REYYKE+ DI+GTLRQLPRY+VAERAILQ LKKCPG
Sbjct: 369  GEWKDAVDMILDPRETERHVVNDAREYYKETGDIDGTLRQLPRYLVAERAILQCLKKCPG 428

Query: 1321 NYLLALKAIPRTLRMMYVHSYQSFLWNHAASTRVQ 1425
            NYL ALK IPRTLRMMYVHSYQS+LWN+AAS RV+
Sbjct: 429  NYLQALKGIPRTLRMMYVHSYQSYLWNNAASLRVK 463