BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020122
         (1542 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002283468.1| PREDICTED: vacuolar amino acid transporter 1...   546   e-153
ref|XP_003633855.1| PREDICTED: vacuolar amino acid transporter 1...   535   e-149
ref|XP_002283458.2| PREDICTED: vacuolar amino acid transporter 1...   525   e-146
ref|XP_003633854.1| PREDICTED: vacuolar amino acid transporter 1...   517   e-144
ref|XP_002323861.1| amino acid transporter [Populus trichocarpa]...   498   e-138

>ref|XP_002283468.1| PREDICTED: vacuolar amino acid transporter 1 [Vitis vinifera]
            gi|296082904|emb|CBI22205.3| unnamed protein product
            [Vitis vinifera]
          Length = 428

 Score =  546 bits (1408), Expect = e-153
 Identities = 274/430 (63%), Positives = 337/430 (78%), Gaps = 7/430 (1%)
 Frame = +1

Query: 187  MDSEQNENGDASVTIPFLHKQQDKEDSETIYSAGR------SSFFNTCFNGLNALSGVGI 348
            M++EQ +    ++T+P LH  Q+    E I S GR      +SFF TCFNGLNALSGVGI
Sbjct: 1    MEAEQRDG--FTLTLPLLHDDQNHNKVEDIESNGRKQDVGSTSFFKTCFNGLNALSGVGI 58

Query: 349  LSTPYALSAGGWLSLPLMFIIATMAFYTALLIQKIMDADPSIRTYPDIGERAFGRKGRML 528
            LSTPYALS+GGWLSL L+ +IAT  FYT LL+++ MDA P+IRTYPD+GERAFG+ GR+L
Sbjct: 59   LSTPYALSSGGWLSLVLLLVIATATFYTGLLLRRCMDAHPNIRTYPDVGERAFGKMGRLL 118

Query: 529  VTSFMNLELYFVAIGFLILEGDNLHNILPD-GIQLNGVKIQGRQSFILIVALVILPTVLL 705
            V+ FM  ELY VA GFLILEGDNLHN+ P+ G+++ G+ I GRQSFI+IV LVILP+V +
Sbjct: 119  VSVFMYTELYLVATGFLILEGDNLHNLFPNMGLEIWGLMIDGRQSFIIIVGLVILPSVWV 178

Query: 706  KNLSILSYISATGVIACAIILGSIMWTGTFDGVGFHERGDFVHLKGIPIAASLYAFCYCG 885
             NL+ILSYISA+GV+AC IILGSI+WTG FDGVGF E+G  ++ +GIP A SLYAFCYC 
Sbjct: 179  NNLNILSYISASGVLACIIILGSILWTGAFDGVGFDEKGTSLNWQGIPTAVSLYAFCYCA 238

Query: 886  HPVFPTLYTSMKNRKNFSKVMFLCFFLCTLTYSSMAVFGYLMFGAEVQSEITLNLPKTKV 1065
            HPVFPTLYTSM+ +  FS V+ +CF  CT+TY++MA+ GYLMFG+ VQS+ITLNLP  K+
Sbjct: 239  HPVFPTLYTSMRKKHQFSNVLLVCFIFCTITYAAMAILGYLMFGSNVQSQITLNLPIEKL 298

Query: 1066 GSRIAIYTTLVTPIAKYALMVTPIVHSIENRFRSYYNKRSFSFLIRIALLISTIVVALAV 1245
             SRIAIYTTLV PI+KYALMVTPIV + EN    YY KR FS +IR  L+ STI+VAL V
Sbjct: 299  SSRIAIYTTLVNPISKYALMVTPIVDATENWLPYYYKKRPFSLIIRSTLVFSTIIVALTV 358

Query: 1246 PFFELLMSLVGAFLSMTASILLPCMCYLKISGAYQGLGIEVGIIGAILIFGVLIVIAGTY 1425
            PFF  LMSLVGAFLS+TASILLPC+CYLKISG Y   G+E+ II  +++ G+   I GTY
Sbjct: 359  PFFGSLMSLVGAFLSVTASILLPCLCYLKISGIYHKFGVELVIIIGVVLMGISAGIMGTY 418

Query: 1426 TSLVQIIAQM 1455
            TS+V+II Q+
Sbjct: 419  TSIVEIIGQL 428


>ref|XP_003633855.1| PREDICTED: vacuolar amino acid transporter 1-like [Vitis vinifera]
            gi|296082909|emb|CBI22210.3| unnamed protein product
            [Vitis vinifera]
          Length = 417

 Score =  535 bits (1378), Expect = e-149
 Identities = 265/407 (65%), Positives = 323/407 (79%), Gaps = 1/407 (0%)
 Frame = +1

Query: 238  LHKQQDKEDSETIYSAGRSSFFNTCFNGLNALSGVGILSTPYALSAGGWLSLPLMFIIAT 417
            L K +D E +      G +SFF TCFNGLNALSGVGILS PYALS+GGWLSL L+ +IAT
Sbjct: 11   LTKVEDIESNGGKQDVGSTSFFKTCFNGLNALSGVGILSIPYALSSGGWLSLVLLLVIAT 70

Query: 418  MAFYTALLIQKIMDADPSIRTYPDIGERAFGRKGRMLVTSFMNLELYFVAIGFLILEGDN 597
              FYT LL+++ MDA P+IRTYPD+GERAFG+KGR+LV+ FM  ELY VA GFLILEGDN
Sbjct: 71   ATFYTGLLLRRCMDAHPNIRTYPDVGERAFGKKGRLLVSVFMYTELYLVATGFLILEGDN 130

Query: 598  LHNILPD-GIQLNGVKIQGRQSFILIVALVILPTVLLKNLSILSYISATGVIACAIILGS 774
            LHN+ P+ G+++ G+ I GRQSFI+IV LVILP+V + NLSILSYISA+GV+AC IILGS
Sbjct: 131  LHNLFPNMGLEIWGLMIDGRQSFIIIVGLVILPSVWVNNLSILSYISASGVLACIIILGS 190

Query: 775  IMWTGTFDGVGFHERGDFVHLKGIPIAASLYAFCYCGHPVFPTLYTSMKNRKNFSKVMFL 954
            I+WTG FDGVGF E+G  ++ +GIP A SLYAFCYC HPVFPTLYTSM+ +  FS V+ +
Sbjct: 191  ILWTGAFDGVGFDEKGTSLNWQGIPTAVSLYAFCYCAHPVFPTLYTSMRKKHQFSNVLLV 250

Query: 955  CFFLCTLTYSSMAVFGYLMFGAEVQSEITLNLPKTKVGSRIAIYTTLVTPIAKYALMVTP 1134
            CF  CT+TY++MA+ GYLMFG+ VQS+ITLNLP  K+ SRIAIYTTLV PI+KYALMVTP
Sbjct: 251  CFIFCTITYAAMAILGYLMFGSNVQSQITLNLPIEKLSSRIAIYTTLVNPISKYALMVTP 310

Query: 1135 IVHSIENRFRSYYNKRSFSFLIRIALLISTIVVALAVPFFELLMSLVGAFLSMTASILLP 1314
            IV++ EN    YY KR FS +IR  L+ STI+VAL VPFF  LMSLVGA LS+ ASILLP
Sbjct: 311  IVNATENWLPYYYKKRPFSLIIRSTLVFSTIIVALTVPFFGSLMSLVGALLSVPASILLP 370

Query: 1315 CMCYLKISGAYQGLGIEVGIIGAILIFGVLIVIAGTYTSLVQIIAQM 1455
            C+CYLKISG Y   G+E+ II  +++ G+   I GTYTS+V+II Q+
Sbjct: 371  CLCYLKISGIYHKFGVELVIIIGVVLMGISAGIMGTYTSIVEIIGQL 417


>ref|XP_002283458.2| PREDICTED: vacuolar amino acid transporter 1-like [Vitis vinifera]
            gi|296082903|emb|CBI22204.3| unnamed protein product
            [Vitis vinifera]
          Length = 428

 Score =  525 bits (1351), Expect = e-146
 Identities = 265/419 (63%), Positives = 321/419 (76%), Gaps = 7/419 (1%)
 Frame = +1

Query: 220  SVTIPFLHKQQDKEDSETIYSAG------RSSFFNTCFNGLNALSGVGILSTPYALSAGG 381
            S+  P LH +    +    YS G       +SFF TCFNGLNALSG+GILS PYAL++GG
Sbjct: 10   SLAFPLLHDESQNVEDMEYYSTGCKLDLGSTSFFKTCFNGLNALSGIGILSVPYALASGG 69

Query: 382  WLSLPLMFIIATMAFYTALLIQKIMDADPSIRTYPDIGERAFGRKGRMLVTSFMNLELYF 561
            WLSL L+F+IA   FYT LL+Q+ MD D +IRTYPDIGE+AFG+KGR++V+ FM LELY 
Sbjct: 70   WLSLMLLFVIALATFYTGLLLQRCMDVDLNIRTYPDIGEQAFGKKGRLMVSIFMYLELYL 129

Query: 562  VAIGFLILEGDNLHNILPD-GIQLNGVKIQGRQSFILIVALVILPTVLLKNLSILSYISA 738
            VA GFLILEGDNLHN+ P  G ++ G  I GRQSFILI  LVILP+V   NL++LSYISA
Sbjct: 130  VATGFLILEGDNLHNLFPMVGFEIFGQVIDGRQSFILISGLVILPSVCFYNLNMLSYISA 189

Query: 739  TGVIACAIILGSIMWTGTFDGVGFHERGDFVHLKGIPIAASLYAFCYCGHPVFPTLYTSM 918
            +GV AC IILGSI+WTG FDGVGFH +G  ++ KGIP A SLYAFCYC HPVFPTLYTSM
Sbjct: 190  SGVFACIIILGSILWTGVFDGVGFHGKGTTLNWKGIPTAFSLYAFCYCAHPVFPTLYTSM 249

Query: 919  KNRKNFSKVMFLCFFLCTLTYSSMAVFGYLMFGAEVQSEITLNLPKTKVGSRIAIYTTLV 1098
            + +  FS V+ + F  CT+TY++MAV GYLMFG+EVQS+ITLNLP  K+ SR+AIYTTLV
Sbjct: 250  RKKNQFSTVLLVGFVFCTITYAAMAVLGYLMFGSEVQSQITLNLPIEKLSSRVAIYTTLV 309

Query: 1099 TPIAKYALMVTPIVHSIENRFRSYYNKRSFSFLIRIALLISTIVVALAVPFFELLMSLVG 1278
             PI+KYAL+V PIV++ EN F    N+R FS LIR AL+ STI+VAL VPFF  LMSLVG
Sbjct: 310  NPISKYALIVVPIVNATENWFPYCCNRRLFSLLIRTALVFSTIIVALTVPFFGSLMSLVG 369

Query: 1279 AFLSMTASILLPCMCYLKISGAYQGLGIEVGIIGAILIFGVLIVIAGTYTSLVQIIAQM 1455
            AFLS+T SILLPC+CYLKISG Y   GIE+ II  +++ G+   I GTYTS+V+II Q+
Sbjct: 370  AFLSVTGSILLPCLCYLKISGIYHKFGIELVIIIGVMLMGISAGIVGTYTSIVEIIGQL 428


>ref|XP_003633854.1| PREDICTED: vacuolar amino acid transporter 1-like [Vitis vinifera]
          Length = 429

 Score =  517 bits (1332), Expect = e-144
 Identities = 266/422 (63%), Positives = 324/422 (76%), Gaps = 10/422 (2%)
 Frame = +1

Query: 220  SVTIPFLHKQ-QDKEDSETIYSA-------GRSSFFNTCFNGLNALSGVGILSTPYALSA 375
            S+  P LH + Q+ ED E  YS+       G +SFF TCFNGLNAL G+GILS PYAL++
Sbjct: 10   SLAFPLLHDESQNVEDME--YSSTGCKLDLGSTSFFKTCFNGLNALLGIGILSVPYALAS 67

Query: 376  GGWLSLPLMFIIATMAFYTALLIQKIMDADPSIRTYPDIGERAFGRKGRMLVTSFMNLEL 555
            GGWLSL L+F+I    FYT LL+Q+ MD D +IRTYPDIGE+AFG+KGR++V+ FM LEL
Sbjct: 68   GGWLSLMLLFVITLATFYTGLLLQRCMDVDLNIRTYPDIGEQAFGKKGRLMVSIFMYLEL 127

Query: 556  YFVAIGFLILEGDNLHNILPD-GIQLNGVKIQGRQSFILIVALVILPTVLLKNLSILSYI 732
            Y VA GFLILEGDNLHN+ P  G ++ G  I GRQSFILI  LVILP+V   NL++LSYI
Sbjct: 128  YLVATGFLILEGDNLHNLFPMVGFEIFGQVIDGRQSFILISGLVILPSVCFYNLNMLSYI 187

Query: 733  SATGVIACAIILGSIMWTGTFDGVGFHERGDFVHLKGIPIAASLYAFCYCGHPVFPTLYT 912
            SA+GV AC IILGSI+WTG FDGVGFH +G  ++ KGIP A SLYAFCYC HPVFPTLYT
Sbjct: 188  SASGVFACIIILGSILWTGVFDGVGFHGKGTTLNWKGIPTAFSLYAFCYCAHPVFPTLYT 247

Query: 913  SMKNRKNFSKVMFL-CFFLCTLTYSSMAVFGYLMFGAEVQSEITLNLPKTKVGSRIAIYT 1089
            SM+ +  FS V+ L CF  CT+ Y++MAV GYLMFG+EVQS+ITLNLP  K+ SR+AIYT
Sbjct: 248  SMRKKNQFSTVILLVCFIFCTIAYAAMAVLGYLMFGSEVQSQITLNLPIEKLSSRVAIYT 307

Query: 1090 TLVTPIAKYALMVTPIVHSIENRFRSYYNKRSFSFLIRIALLISTIVVALAVPFFELLMS 1269
            TLV PI+KYALMV PIV++ EN F +Y N+RSFS LIR AL+ STI+VAL VPFF  LMS
Sbjct: 308  TLVNPISKYALMVAPIVNATENCFPNYCNRRSFSLLIRTALVFSTIIVALTVPFFGSLMS 367

Query: 1270 LVGAFLSMTASILLPCMCYLKISGAYQGLGIEVGIIGAILIFGVLIVIAGTYTSLVQIIA 1449
            +V AF ++T SILLPC+CYLKISG Y   GIE+ I+  +++ G    I GTYTS+V+II 
Sbjct: 368  MVVAFSTVTGSILLPCLCYLKISGIYHKFGIELVIMIGVMLMGTSAGIMGTYTSIVEIIG 427

Query: 1450 QM 1455
            Q+
Sbjct: 428  QL 429


>ref|XP_002323861.1| amino acid transporter [Populus trichocarpa]
            gi|222866863|gb|EEF03994.1| amino acid transporter
            [Populus trichocarpa]
          Length = 388

 Score =  498 bits (1282), Expect = e-138
 Identities = 251/388 (64%), Positives = 308/388 (79%), Gaps = 1/388 (0%)
 Frame = +1

Query: 286  GRSSFFNTCFNGLNALSGVGILSTPYALSAGGWLSLPLMFIIATMAFYTALLIQKIMDAD 465
            G +S F T F+GLNALSGVGILS PYALS+GGWLSL L+ +I+T AFYT LLIQ+ MD D
Sbjct: 1    GSTSTFKTVFHGLNALSGVGILSIPYALSSGGWLSLILLCVISTAAFYTGLLIQRCMDLD 60

Query: 466  PSIRTYPDIGERAFGRKGRMLVTSFMNLELYFVAIGFLILEGDNLHNILPD-GIQLNGVK 642
             +IRTYPDIGERAFG+KGR+LV+  M  ELY V+ GFLILEGDNL N+ P+ GI++ G +
Sbjct: 61   SNIRTYPDIGERAFGKKGRILVSVLMYTELYLVSTGFLILEGDNLQNLFPNMGIEVAGFE 120

Query: 643  IQGRQSFILIVALVILPTVLLKNLSILSYISATGVIACAIILGSIMWTGTFDGVGFHERG 822
            I GRQS ++IVAL+ILPTV L ++SILSYISA+GVIA AI+L SI WTG FDGVGF E+G
Sbjct: 121  IGGRQSSVIIVALIILPTVWLDDMSILSYISASGVIASAIVLISIFWTGAFDGVGFKEKG 180

Query: 823  DFVHLKGIPIAASLYAFCYCGHPVFPTLYTSMKNRKNFSKVMFLCFFLCTLTYSSMAVFG 1002
              V+  GIP A SLYAFCYC HPVFPTLYTSMKN+  FS VM LCF LC+L+Y+SMAV G
Sbjct: 181  TLVNWHGIPTAVSLYAFCYCAHPVFPTLYTSMKNKHQFSNVMILCFILCSLSYASMAVMG 240

Query: 1003 YLMFGAEVQSEITLNLPKTKVGSRIAIYTTLVTPIAKYALMVTPIVHSIENRFRSYYNKR 1182
            YLMFG  VQS+ITLNLP  K+ S++ IYTTLV+PI KYALM+ PIV++ ++       KR
Sbjct: 241  YLMFGPSVQSQITLNLPTEKLSSKLTIYTTLVSPITKYALMIIPIVNATKSWLPMNGKKR 300

Query: 1183 SFSFLIRIALLISTIVVALAVPFFELLMSLVGAFLSMTASILLPCMCYLKISGAYQGLGI 1362
             FS  I  AL+IS ++VAL++PFF  LMSLVGAFLSMTASI+LP +CY+KISG YQ  G 
Sbjct: 301  PFSLFIGTALVISNVIVALSLPFFGDLMSLVGAFLSMTASIILPSLCYMKISGTYQRFGF 360

Query: 1363 EVGIIGAILIFGVLIVIAGTYTSLVQII 1446
            E+ ++  +++ GV +VI GTYTSL++II
Sbjct: 361  EMVVLWTVVLLGVAVVILGTYTSLLEII 388