BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020088
         (500 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_974786.1| prenylcysteine methylesterase [Arabidopsis thal...   168   4e-40
ref|NP_197090.2| prenylcysteine methylesterase [Arabidopsis thal...   168   4e-40
ref|XP_002873746.1| predicted protein [Arabidopsis lyrata subsp....   164   5e-39
ref|XP_002533641.1| carboxylesterase, putative [Ricinus communis...   159   2e-37
ref|XP_002264962.1| PREDICTED: isoprenylcysteine alpha-carbonyl ...   155   3e-36

>ref|NP_974786.1| prenylcysteine methylesterase [Arabidopsis thaliana]
           gi|332004835|gb|AED92218.1| prenylcysteine
           methylesterase [Arabidopsis thaliana]
          Length = 299

 Score =  168 bits (426), Expect = 4e-40
 Identities = 90/163 (55%), Positives = 108/163 (66%)
 Frame = +2

Query: 11  SSPEKIDQLSDDTNNPDDQLIGSDENHQLRRRRKAKDDGKETPRRMTLLERRNSLTHEIG 190
           S+  +I+++  D ++    L+  +    LRRR   K    E PRR+    R+ S   +IG
Sbjct: 19  STVSEIEEVLPDEDSDRTTLLNGEP---LRRRVSGKSPVDEGPRRIF---RQQSFGRDIG 72

Query: 191 HAAAETYLITGLAFKLLAYLGVGYRWISKXXXXXXXXXXXXXXXIQVGYNYFFSSQVQRS 370
           HAAAETYLITGL+FKLL YLGVGYRW++K               +QV Y+YFFS QV+RS
Sbjct: 73  HAAAETYLITGLSFKLLRYLGVGYRWMTKLLALTCYAMLLMPGFLQVAYSYFFSKQVRRS 132

Query: 371 IVYGDFRRNRLDLYLPKNMDGLKPVVAFVTGGAWTIGYKAWGS 499
           IVYGD  RNRLDLYLP N DGLKPVV FVTGGAW IGYKAWGS
Sbjct: 133 IVYGDQPRNRLDLYLPSNNDGLKPVVVFVTGGAWIIGYKAWGS 175


>ref|NP_197090.2| prenylcysteine methylesterase [Arabidopsis thaliana]
           gi|75332056|sp|Q94AS5.1|ICME_ARATH RecName:
           Full=Isoprenylcysteine alpha-carbonyl methylesterase
           ICME; AltName: Full=Isoprenylcysteine methylesterase;
           AltName: Full=Prenylcysteine methylesterase;
           Short=AtPCME gi|15027945|gb|AAK76503.1| putative
           Carboxylesterase [Arabidopsis thaliana]
           gi|20259181|gb|AAM14306.1| putative carboxylesterase
           [Arabidopsis thaliana] gi|332004834|gb|AED92217.1|
           prenylcysteine methylesterase [Arabidopsis thaliana]
          Length = 427

 Score =  168 bits (426), Expect = 4e-40
 Identities = 90/163 (55%), Positives = 108/163 (66%)
 Frame = +2

Query: 11  SSPEKIDQLSDDTNNPDDQLIGSDENHQLRRRRKAKDDGKETPRRMTLLERRNSLTHEIG 190
           S+  +I+++  D ++    L+  +    LRRR   K    E PRR+    R+ S   +IG
Sbjct: 19  STVSEIEEVLPDEDSDRTTLLNGEP---LRRRVSGKSPVDEGPRRIF---RQQSFGRDIG 72

Query: 191 HAAAETYLITGLAFKLLAYLGVGYRWISKXXXXXXXXXXXXXXXIQVGYNYFFSSQVQRS 370
           HAAAETYLITGL+FKLL YLGVGYRW++K               +QV Y+YFFS QV+RS
Sbjct: 73  HAAAETYLITGLSFKLLRYLGVGYRWMTKLLALTCYAMLLMPGFLQVAYSYFFSKQVRRS 132

Query: 371 IVYGDFRRNRLDLYLPKNMDGLKPVVAFVTGGAWTIGYKAWGS 499
           IVYGD  RNRLDLYLP N DGLKPVV FVTGGAW IGYKAWGS
Sbjct: 133 IVYGDQPRNRLDLYLPSNNDGLKPVVVFVTGGAWIIGYKAWGS 175


>ref|XP_002873746.1| predicted protein [Arabidopsis lyrata subsp. lyrata]
           gi|297319583|gb|EFH50005.1| predicted protein
           [Arabidopsis lyrata subsp. lyrata]
          Length = 427

 Score =  164 bits (416), Expect = 5e-39
 Identities = 89/163 (54%), Positives = 107/163 (65%)
 Frame = +2

Query: 11  SSPEKIDQLSDDTNNPDDQLIGSDENHQLRRRRKAKDDGKETPRRMTLLERRNSLTHEIG 190
           S+  +I+++  D ++    L+  +    LRRR        E PRR+    R+ S   +IG
Sbjct: 19  STVSEIEEVIPDEDSDRTTLLNGEP---LRRRVSGNLRVDEGPRRIF---RQQSFGRDIG 72

Query: 191 HAAAETYLITGLAFKLLAYLGVGYRWISKXXXXXXXXXXXXXXXIQVGYNYFFSSQVQRS 370
           HAAAETYLITGL+FKLL YLGVGYRW++K               +QV Y+YFFS QV+RS
Sbjct: 73  HAAAETYLITGLSFKLLRYLGVGYRWMTKLLALTCYAMLLMPGFLQVAYSYFFSKQVRRS 132

Query: 371 IVYGDFRRNRLDLYLPKNMDGLKPVVAFVTGGAWTIGYKAWGS 499
           IVYGD  RNRLDLYLP N DGLKPVV FVTGGAW IGYKAWGS
Sbjct: 133 IVYGDQPRNRLDLYLPSNNDGLKPVVVFVTGGAWIIGYKAWGS 175


>ref|XP_002533641.1| carboxylesterase, putative [Ricinus communis]
           gi|223526470|gb|EEF28744.1| carboxylesterase, putative
           [Ricinus communis]
          Length = 429

 Score =  159 bits (403), Expect = 2e-37
 Identities = 86/173 (49%), Positives = 105/173 (60%), Gaps = 7/173 (4%)
 Frame = +2

Query: 2   ASPSSPEKIDQLSDDTNNPDDQLIGSDENHQLRRRRKAKDDGKETPRRMTLL-------E 160
           A+P SP+        +++P      + EN    +      + K+ PRR           E
Sbjct: 8   AAPISPDLHRSFPPSSSSPSS----NGENISNSKMNNGGGNTKQQPRRRVSAAGRPAKPE 63

Query: 161 RRNSLTHEIGHAAAETYLITGLAFKLLAYLGVGYRWISKXXXXXXXXXXXXXXXIQVGYN 340
           R+ S + +IGHAA ETYL+T L F LL YLGVGYRWI++               +QV Y 
Sbjct: 64  RQQSFSRDIGHAACETYLLTRLTFTLLRYLGVGYRWITRLAALAFYAFLLMPGFLQVAYY 123

Query: 341 YFFSSQVQRSIVYGDFRRNRLDLYLPKNMDGLKPVVAFVTGGAWTIGYKAWGS 499
           YFFSSQV+RSI+YGD  RNRLDLYLP+NMDG KPVVAFVTGGAW IGYKAWGS
Sbjct: 124 YFFSSQVRRSIIYGDQPRNRLDLYLPENMDGPKPVVAFVTGGAWIIGYKAWGS 176


>ref|XP_002264962.1| PREDICTED: isoprenylcysteine alpha-carbonyl methylesterase ICME
           [Vitis vinifera] gi|296087101|emb|CBI33475.3| unnamed
           protein product [Vitis vinifera]
          Length = 417

 Score =  155 bits (392), Expect = 3e-36
 Identities = 80/160 (50%), Positives = 102/160 (63%)
 Frame = +2

Query: 17  PEKIDQLSDDTNNPDDQLIGSDENHQLRRRRKAKDDGKETPRRMTLLERRNSLTHEIGHA 196
           P  +  +  DTN     ++        ++ R+ +   + +P     L RR S + +IGHA
Sbjct: 5   PATMPDVDMDTNPLLSGVLSHTNMGTTKKHRRRRSASRPSPAPSQPL-RRQSFSRDIGHA 63

Query: 197 AAETYLITGLAFKLLAYLGVGYRWISKXXXXXXXXXXXXXXXIQVGYNYFFSSQVQRSIV 376
           AAETYL+T L+FKLL YLGVGYRW+++               ++V Y YFFSSQV+RS+V
Sbjct: 64  AAETYLVTRLSFKLLRYLGVGYRWMTRLVALGCYAMLLMPGFLRVAYYYFFSSQVRRSVV 123

Query: 377 YGDFRRNRLDLYLPKNMDGLKPVVAFVTGGAWTIGYKAWG 496
           YGD  RNRLDLYLP+N DG KPVVAFVTGGAW IGYKAWG
Sbjct: 124 YGDQPRNRLDLYLPENNDGPKPVVAFVTGGAWIIGYKAWG 163