BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00020010
         (977 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel prot...   471   e-130
ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel prot...   471   e-130
ref|NP_001159287.1| uncharacterized protein LOC100304377 [Zea ma...   471   e-130
ref|XP_002454503.1| hypothetical protein SORBIDRAFT_04g032300 [S...   467   e-129
ref|XP_003570063.1| PREDICTED: uncharacterized mscS family prote...   464   e-128

>ref|XP_004170243.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
            sativus]
          Length = 923

 Score =  471 bits (1212), Expect = e-130
 Identities = 226/324 (69%), Positives = 271/324 (83%)
 Frame = +1

Query: 4    ITIDHLHRLNQKNISAWNMKRLMNIIRKGTLSTLDEQLQVPTDDDESSVQITSEYQAKVA 183
            ITIDHLH+L+ KN+SAWNMKRLMNI+R GTLSTLDEQ++    +DES+ +I SEY+AKVA
Sbjct: 554  ITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVA 613

Query: 184  AKKIFSNVAKPGSKYIYEEDLMRFMREDEASRTIRLFEGAAEGKGISRRALKNWVVNAFR 363
            AKKIF NVA+ GSKYIY EDLMRFM +DEAS+T+ LFEGA E + IS+ +LKNWVVNAFR
Sbjct: 614  AKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFR 673

Query: 364  ERRALALSLNDTKTAVNKLHQMXXXXXXXXXXXXXXXXXXXANTHFFVLISSQLLLVVFV 543
            ERRALAL+LNDTKTAVNKLH+M                   A + F + ++SQL+LV FV
Sbjct: 674  ERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFV 733

Query: 544  FGNTCKTTFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMNILTTIFLRYDNQKISYPNSI 723
            FGNTCKT FE+IIFLFVMHPFDVGDRCE+DGVQM+VEEMNILTTIFLRYDNQKI +PNS+
Sbjct: 734  FGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSV 793

Query: 724  LATKPISNFYRSPDMGDAIDFCIHISTPMDKIAMMKERITRYIENKSDHWYPAPLIVLRD 903
            LATK I NFYRSPDMGD I+FC+HISTP +KIA+M++RI  YIE K +HW PAP+IVL+D
Sbjct: 794  LATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKD 853

Query: 904  VDDMNRLKISIWLSHRMNFQDMGE 975
            V+++NR++I+IWL+HRMN QDMGE
Sbjct: 854  VEELNRMRIAIWLTHRMNHQDMGE 877


>ref|XP_004140046.1| PREDICTED: mechanosensitive ion channel protein 8-like [Cucumis
            sativus]
          Length = 923

 Score =  471 bits (1212), Expect = e-130
 Identities = 226/324 (69%), Positives = 271/324 (83%)
 Frame = +1

Query: 4    ITIDHLHRLNQKNISAWNMKRLMNIIRKGTLSTLDEQLQVPTDDDESSVQITSEYQAKVA 183
            ITIDHLH+L+ KN+SAWNMKRLMNI+R GTLSTLDEQ++    +DES+ +I SEY+AKVA
Sbjct: 554  ITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTLDEQIKDTAHEDESTTEIKSEYEAKVA 613

Query: 184  AKKIFSNVAKPGSKYIYEEDLMRFMREDEASRTIRLFEGAAEGKGISRRALKNWVVNAFR 363
            AKKIF NVA+ GSKYIY EDLMRFM +DEAS+T+ LFEGA E + IS+ +LKNWVVNAFR
Sbjct: 614  AKKIFLNVARNGSKYIYLEDLMRFMEKDEASKTMGLFEGACESRKISKSSLKNWVVNAFR 673

Query: 364  ERRALALSLNDTKTAVNKLHQMXXXXXXXXXXXXXXXXXXXANTHFFVLISSQLLLVVFV 543
            ERRALAL+LNDTKTAVNKLH+M                   A + F + ++SQL+LV FV
Sbjct: 674  ERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWLLILGIATSKFLLFVTSQLVLVAFV 733

Query: 544  FGNTCKTTFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMNILTTIFLRYDNQKISYPNSI 723
            FGNTCKT FE+IIFLFVMHPFDVGDRCE+DGVQM+VEEMNILTTIFLRYDNQKI +PNS+
Sbjct: 734  FGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSV 793

Query: 724  LATKPISNFYRSPDMGDAIDFCIHISTPMDKIAMMKERITRYIENKSDHWYPAPLIVLRD 903
            LATK I NFYRSPDMGD I+FC+HISTP +KIA+M++RI  YIE K +HW PAP+IVL+D
Sbjct: 794  LATKAIHNFYRSPDMGDGIEFCLHISTPPEKIAIMRQRIISYIEGKKEHWCPAPMIVLKD 853

Query: 904  VDDMNRLKISIWLSHRMNFQDMGE 975
            V+++NR++I+IWL+HRMN QDMGE
Sbjct: 854  VEELNRMRIAIWLTHRMNHQDMGE 877


>ref|NP_001159287.1| uncharacterized protein LOC100304377 [Zea mays]
            gi|223943213|gb|ACN25690.1| unknown [Zea mays]
            gi|413938170|gb|AFW72721.1| hypothetical protein
            ZEAMMB73_243815 [Zea mays]
          Length = 966

 Score =  471 bits (1211), Expect = e-130
 Identities = 228/324 (70%), Positives = 269/324 (83%), Gaps = 1/324 (0%)
 Frame = +1

Query: 4    ITIDHLHRLNQKNISAWNMKRLMNIIRKGTLSTLDEQLQVPTDD-DESSVQITSEYQAKV 180
            I+ID LH+LNQKNISAWNMKRLM I+R GTL+T+DEQ+Q  T + DES+ QI SEY+AK+
Sbjct: 592  ISIDALHKLNQKNISAWNMKRLMRIVRFGTLTTMDEQIQQATGEGDESATQIRSEYEAKI 651

Query: 181  AAKKIFSNVAKPGSKYIYEEDLMRFMREDEASRTIRLFEGAAEGKGISRRALKNWVVNAF 360
            AAKKIF NVAKPGSKYIY  DL+RFMR++EA + + LFEGA E   +S+R+LKNWVVNAF
Sbjct: 652  AAKKIFHNVAKPGSKYIYLSDLIRFMRQEEAVKAMNLFEGAQEHNRVSKRSLKNWVVNAF 711

Query: 361  RERRALALSLNDTKTAVNKLHQMXXXXXXXXXXXXXXXXXXXANTHFFVLISSQLLLVVF 540
            RER+ALAL+LNDTKTAVNKL+QM                   A THFFV +SSQLLL VF
Sbjct: 712  RERKALALTLNDTKTAVNKLNQMVNVVVGIIVFVLWLLILGIATTHFFVFLSSQLLLAVF 771

Query: 541  VFGNTCKTTFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMNILTTIFLRYDNQKISYPNS 720
            VFGNT KT FEAI+FLFVMHPFDVGDRCE++GVQ VVEEMNI+TT+FLRYDN KI YPNS
Sbjct: 772  VFGNTLKTVFEAIVFLFVMHPFDVGDRCEIEGVQAVVEEMNIMTTVFLRYDNLKIYYPNS 831

Query: 721  ILATKPISNFYRSPDMGDAIDFCIHISTPMDKIAMMKERITRYIENKSDHWYPAPLIVLR 900
            +LATKPI NFYRSPDMG+AIDF IH+STP++K+A+MKERI RYI+NK +HWYP  ++VLR
Sbjct: 832  VLATKPIMNFYRSPDMGEAIDFSIHVSTPVEKLALMKERILRYIDNKKEHWYPGAMVVLR 891

Query: 901  DVDDMNRLKISIWLSHRMNFQDMG 972
            DVDD N+LK+SIWL H +NFQDMG
Sbjct: 892  DVDDTNKLKVSIWLRHTLNFQDMG 915


>ref|XP_002454503.1| hypothetical protein SORBIDRAFT_04g032300 [Sorghum bicolor]
            gi|241934334|gb|EES07479.1| hypothetical protein
            SORBIDRAFT_04g032300 [Sorghum bicolor]
          Length = 1050

 Score =  467 bits (1202), Expect = e-129
 Identities = 225/324 (69%), Positives = 269/324 (83%), Gaps = 1/324 (0%)
 Frame = +1

Query: 4    ITIDHLHRLNQKNISAWNMKRLMNIIRKGTLSTLDEQLQVPTDD-DESSVQITSEYQAKV 180
            I+ID LH+LNQKNISAWNMKRLM I+R GTL+T+DEQ+Q  T   DES+ QI SEY+AK+
Sbjct: 676  ISIDALHKLNQKNISAWNMKRLMRIVRFGTLTTMDEQIQQATGQGDESATQIRSEYEAKI 735

Query: 181  AAKKIFSNVAKPGSKYIYEEDLMRFMREDEASRTIRLFEGAAEGKGISRRALKNWVVNAF 360
            AAKKIF NVAKPGSKYIY  D+MRFMR++EA + + LFEGA E   +S+R+LKNWVVNAF
Sbjct: 736  AAKKIFHNVAKPGSKYIYLSDMMRFMRQEEAVKAMDLFEGAQEHNRVSKRSLKNWVVNAF 795

Query: 361  RERRALALSLNDTKTAVNKLHQMXXXXXXXXXXXXXXXXXXXANTHFFVLISSQLLLVVF 540
            RER+ALAL+LNDTKTAVNKL+QM                   A THFFV +SSQLLL VF
Sbjct: 796  RERKALALTLNDTKTAVNKLNQMANVVVGIIVFALWLLILGIATTHFFVFLSSQLLLAVF 855

Query: 541  VFGNTCKTTFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMNILTTIFLRYDNQKISYPNS 720
            VFGNT KT FEAI+FLFVMHPFDVGDRCE++GVQMVVEEMNI+TT+FLRYDN KI YPNS
Sbjct: 856  VFGNTLKTVFEAIVFLFVMHPFDVGDRCEIEGVQMVVEEMNIMTTVFLRYDNLKIYYPNS 915

Query: 721  ILATKPISNFYRSPDMGDAIDFCIHISTPMDKIAMMKERITRYIENKSDHWYPAPLIVLR 900
            +LATKPI N+YRSPDMG+AIDF IH++TP++K+A+MKER+ RYI+NK +HWYP  ++VLR
Sbjct: 916  VLATKPIMNYYRSPDMGEAIDFSIHVATPVEKLALMKERLLRYIDNKKEHWYPGAMVVLR 975

Query: 901  DVDDMNRLKISIWLSHRMNFQDMG 972
            DVDD N+LK+SIWL H +N+QDMG
Sbjct: 976  DVDDTNKLKVSIWLRHTLNWQDMG 999


>ref|XP_003570063.1| PREDICTED: uncharacterized mscS family protein At1g78610-like
            [Brachypodium distachyon]
          Length = 959

 Score =  464 bits (1195), Expect = e-128
 Identities = 225/324 (69%), Positives = 268/324 (82%), Gaps = 1/324 (0%)
 Frame = +1

Query: 4    ITIDHLHRLNQKNISAWNMKRLMNIIRKGTLSTLDEQLQVPTDD-DESSVQITSEYQAKV 180
            ITID LHRLNQKN+SAWNMKRLM I+R GTL+T+DEQ+Q  T + DES+ QI SEY+A++
Sbjct: 585  ITIDKLHRLNQKNVSAWNMKRLMKIVRFGTLTTMDEQIQQATGEGDESATQIRSEYEAQI 644

Query: 181  AAKKIFSNVAKPGSKYIYEEDLMRFMREDEASRTIRLFEGAAEGKGISRRALKNWVVNAF 360
            AAKKIF+NVAKPGSKYIY  DLMRFMR++EA + + LFEGA E   +S+R+LKNWVV AF
Sbjct: 645  AAKKIFNNVAKPGSKYIYLADLMRFMRQEEAIKAMHLFEGAQEHCRVSKRSLKNWVVTAF 704

Query: 361  RERRALALSLNDTKTAVNKLHQMXXXXXXXXXXXXXXXXXXXANTHFFVLISSQLLLVVF 540
            RER+ALAL+LNDTKTAVNKL+QM                   A THFFV +SSQLL+ VF
Sbjct: 705  RERKALALTLNDTKTAVNKLNQMTNIIVGVIVFALWLLILGIATTHFFVFLSSQLLVAVF 764

Query: 541  VFGNTCKTTFEAIIFLFVMHPFDVGDRCEVDGVQMVVEEMNILTTIFLRYDNQKISYPNS 720
            VFGNT KT FEAIIFLFVMHPFDVGDRCE++ VQ+VVEEMNI+TT+FLRYDN KI YPNS
Sbjct: 765  VFGNTMKTIFEAIIFLFVMHPFDVGDRCEIEEVQLVVEEMNIMTTVFLRYDNLKIYYPNS 824

Query: 721  ILATKPISNFYRSPDMGDAIDFCIHISTPMDKIAMMKERITRYIENKSDHWYPAPLIVLR 900
            +LATKPI NFYRSPDMG+ IDF IH++TP++K+A+MKERI RYI+NK +HWYP  ++VLR
Sbjct: 825  VLATKPIMNFYRSPDMGEGIDFSIHVATPVEKLALMKERILRYIDNKKEHWYPGAMVVLR 884

Query: 901  DVDDMNRLKISIWLSHRMNFQDMG 972
            DVDD N+LK+SIWL H +NFQDMG
Sbjct: 885  DVDDTNKLKVSIWLRHTLNFQDMG 908