BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00019842
         (1333 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI22452.3| unnamed protein product [Vitis vinifera]              258   1e-90
ref|XP_002264518.1| PREDICTED: DNA polymerase kappa-like [Vitis ...   258   3e-66
ref|XP_003610613.1| DNA polymerase IV [Medicago truncatula] gi|3...   220   6e-55
dbj|BAD93992.1| hypothetical protein [Arabidopsis thaliana]           219   1e-54
ref|NP_175420.3| DNA polymerase kappa subunit [Arabidopsis thali...   219   1e-54

>emb|CBI22452.3| unnamed protein product [Vitis vinifera]
          Length = 704

 Score =  258 bits (658), Expect(2) = 1e-90
 Identities = 132/190 (69%), Positives = 154/190 (81%)
 Frame = -1

Query: 1333 FLCALFSHSSADFFLSVGLGLGGTDTPQAKSRKSISNERTFSATEDEAVLYRKIVELAEM 1154
            FLCALFS SSADFFLSVGLGLG TDTPQ + RKSISNERTFSAT+D A+LY+K+ EL+EM
Sbjct: 312  FLCALFSRSSADFFLSVGLGLGKTDTPQVRFRKSISNERTFSATDDTALLYQKLAELSEM 371

Query: 1153 LSVDMKKEGLSGRTLTLKLKTASFEVRTRAVTLQNYIYSSEDILSHASKLLKAELPISIR 974
            LS+DM+KEGLSGRTLTLKLKTASFEVR+RAVT+Q YI S EDIL+HA KLL AELP+S+R
Sbjct: 372  LSIDMQKEGLSGRTLTLKLKTASFEVRSRAVTMQKYICSREDILNHALKLLNAELPLSLR 431

Query: 973  LMGLRMSHFREDHDGFLADPTQKTLSSFFIPEDAKKRNTDANDSLGADVDGSLLANNEEA 794
            L+GLR+SHF ED DG L+DPTQKTL++F I  DA  +N   +  LG+D   S    + E 
Sbjct: 432  LIGLRISHFNEDKDGHLSDPTQKTLANFIISGDASGKNVCEHSLLGSDTSDSHFMVDPEN 491

Query: 793  GLPIDNHELC 764
             L IDNHE C
Sbjct: 492  DLSIDNHETC 501



 Score =  103 bits (257), Expect(2) = 1e-90
 Identities = 71/165 (43%), Positives = 90/165 (54%), Gaps = 16/165 (9%)
 Frame = -3

Query: 644  NGTEVKDNSLNNTRDPPKSYELDESQLFETKTLSRLVDKSCIN------QQSQMDLLSYD 483
            NG+  K N+ N     PKS+++  S L E  + S    + C +      +++  DL   D
Sbjct: 540  NGSVAKVNAPNVVGCAPKSHQIKVSHLVEADSFS--FSEQCESNRPDRARRTNNDL--DD 595

Query: 482  AGSSSNYKESSLWVDDYKCVLCGSEIPLIFAEERQEHSDFHLAERLQAEESDNYNDNRTL 303
               SSN KE  LWV+DYKC LCG E+P  F EERQEH DFHLAE+LQ EESDN + N  L
Sbjct: 596  GVPSSNQKEQFLWVNDYKCSLCGIELPPNFVEERQEHFDFHLAEKLQKEESDN-SRNLML 654

Query: 302  KHRVID---AASQGRGKKKQ-------KLSPSAGKHVPIDVFFSK 198
              R       ASQ R K+ +       K S    KH+PID+FF K
Sbjct: 655  NQRFSQRDHTASQRRHKRHKSSKKEGSKSSQKESKHLPIDMFFVK 699


>ref|XP_002264518.1| PREDICTED: DNA polymerase kappa-like [Vitis vinifera]
          Length = 661

 Score =  258 bits (658), Expect = 3e-66
 Identities = 132/190 (69%), Positives = 154/190 (81%)
 Frame = -1

Query: 1333 FLCALFSHSSADFFLSVGLGLGGTDTPQAKSRKSISNERTFSATEDEAVLYRKIVELAEM 1154
            FLCALFS SSADFFLSVGLGLG TDTPQ + RKSISNERTFSAT+D A+LY+K+ EL+EM
Sbjct: 312  FLCALFSRSSADFFLSVGLGLGKTDTPQVRFRKSISNERTFSATDDTALLYQKLAELSEM 371

Query: 1153 LSVDMKKEGLSGRTLTLKLKTASFEVRTRAVTLQNYIYSSEDILSHASKLLKAELPISIR 974
            LS+DM+KEGLSGRTLTLKLKTASFEVR+RAVT+Q YI S EDIL+HA KLL AELP+S+R
Sbjct: 372  LSIDMQKEGLSGRTLTLKLKTASFEVRSRAVTMQKYICSREDILNHALKLLNAELPLSLR 431

Query: 973  LMGLRMSHFREDHDGFLADPTQKTLSSFFIPEDAKKRNTDANDSLGADVDGSLLANNEEA 794
            L+GLR+SHF ED DG L+DPTQKTL++F I  DA  +N   +  LG+D   S    + E 
Sbjct: 432  LIGLRISHFNEDKDGHLSDPTQKTLANFIISGDASGKNVCEHSLLGSDTSDSHFMVDPEN 491

Query: 793  GLPIDNHELC 764
             L IDNHE C
Sbjct: 492  DLSIDNHETC 501



 Score = 95.1 bits (235), Expect = 4e-17
 Identities = 50/96 (52%), Positives = 59/96 (61%)
 Frame = -3

Query: 485 DAGSSSNYKESSLWVDDYKCVLCGSEIPLIFAEERQEHSDFHLAERLQAEESDNYNDNRT 306
           D   SSN KE  LWV+DYKC LCG E+P  F EERQEH DFHLAE+LQ EESDN  +   
Sbjct: 563 DGVPSSNQKEQFLWVNDYKCSLCGIELPPNFVEERQEHFDFHLAEKLQKEESDNSRNLTA 622

Query: 305 LKHRVIDAASQGRGKKKQKLSPSAGKHVPIDVFFSK 198
            + R      +   K+  K S    KH+PID+FF K
Sbjct: 623 SQRR--HKRHKSSKKEGSKSSQKESKHLPIDMFFVK 656


>ref|XP_003610613.1| DNA polymerase IV [Medicago truncatula] gi|355511948|gb|AES93571.1|
            DNA polymerase IV [Medicago truncatula]
          Length = 706

 Score =  220 bits (561), Expect = 6e-55
 Identities = 120/196 (61%), Positives = 145/196 (73%), Gaps = 1/196 (0%)
 Frame = -1

Query: 1333 FLCALFSHSSADFFLSVGLGLGGTDTPQAKSRKSISNERTFSATEDEAVLYRKIVELAEM 1154
            +L ALFS S+ADFF SVGLGLG TD+PQ + RKSISNERTFSA+EDEA+L++K+ ELAEM
Sbjct: 333  YLSALFSQSTADFFYSVGLGLGKTDSPQVRFRKSISNERTFSASEDEALLHKKLAELAEM 392

Query: 1153 LSVDMKKEGLSGRTLTLKLKTASFEVRTRAVTLQNYIYSSEDILSHASKLLKAELPISIR 974
            LS DM+KEGL GRTLTLKLKTASFEVR RAVTLQNYI SS+DIL HASKLLKAELP+S+R
Sbjct: 393  LSTDMQKEGLHGRTLTLKLKTASFEVRNRAVTLQNYINSSDDILKHASKLLKAELPVSVR 452

Query: 973  LMGLRMSHFREDHDGFLADPTQKTLSSFFIPEDAKKRNTDANDSLGADVDGSLLANNEEA 794
            L+GLR+S F     G   DPTQKT+++F    +A ++N+  +D    D        + E 
Sbjct: 453  LIGLRVSQFSGQKCGATPDPTQKTITNFITSGEANRKNSSFSDVTDHD-----FVTDTET 507

Query: 793  GLPID-NHELCCDLRD 749
             L +D  H    D RD
Sbjct: 508  DLSLDGRHTGQLDSRD 523



 Score = 66.6 bits (161), Expect = 1e-08
 Identities = 29/40 (72%), Positives = 32/40 (80%)
 Frame = -3

Query: 449 LWVDDYKCVLCGSEIPLIFAEERQEHSDFHLAERLQAEES 330
           LW+DDYKC LCG+E+P  F EER EHSDFH AE LQ EES
Sbjct: 604 LWLDDYKCSLCGAELPPSFVEERLEHSDFHFAELLQKEES 643


>dbj|BAD93992.1| hypothetical protein [Arabidopsis thaliana]
          Length = 402

 Score =  219 bits (558), Expect = 1e-54
 Identities = 116/189 (61%), Positives = 148/189 (78%), Gaps = 2/189 (1%)
 Frame = -1

Query: 1330 LCALFSHSSADFFLSVGLGLGGTDTPQAKSRKSISNERTFSATEDEAVLYRKIVELAEML 1151
            L ALFS SSADFFLSVGLGLGGT+TPQ +SRKSIS+ERTF+AT DE +LY K+ ELAEML
Sbjct: 42   LYALFSQSSADFFLSVGLGLGGTNTPQVRSRKSISSERTFAATGDERLLYSKLDELAEML 101

Query: 1150 SVDMKKEGLSGRTLTLKLKTASFEVRTRAVTLQNYIYSSEDILSHASKLLKAELPISIRL 971
            S DMKKEGL+ RTLTLKLKTASFE+R+RAV+LQ Y  SS+DIL HA+KLLKAELP+S+RL
Sbjct: 102  SHDMKKEGLTARTLTLKLKTASFEIRSRAVSLQRYTCSSDDILKHATKLLKAELPVSVRL 161

Query: 970  MGLRMSHFREDHDGFLADPTQKTLSSFFIPEDAKK--RNTDANDSLGADVDGSLLANNEE 797
            +GLRMS F E+     +DP+Q T++ F + +D+ +  ++ D NDS   D + + L+N+E 
Sbjct: 162  IGLRMSQFVEEIRN--SDPSQGTITKFIVQKDSSRQAQDLDDNDSFDLDANKNCLSNDES 219

Query: 796  AGLPIDNHE 770
              +   +HE
Sbjct: 220  GNVSFGSHE 228



 Score = 88.2 bits (217), Expect = 4e-15
 Identities = 43/88 (48%), Positives = 54/88 (61%), Gaps = 1/88 (1%)
 Frame = -3

Query: 458 ESSLWVDDYKCVLCGSEIPLIFAEERQEHSDFHLAERLQAEESDNYNDNRTLKHRVIDAA 279
           E   W+D YKCVLCG E+P  F EERQEHSDFHLA+RLQ EE+ + +     K R++   
Sbjct: 304 EPLYWIDGYKCVLCGIELPPSFVEERQEHSDFHLAQRLQNEETGSSSSTTPSKRRILGKE 363

Query: 278 SQGRGKKKQKL-SPSAGKHVPIDVFFSK 198
                 KKQK     + KH+PI  FF+K
Sbjct: 364 KVNSKPKKQKPDQKDSSKHIPIHAFFTK 391


>ref|NP_175420.3| DNA polymerase kappa subunit [Arabidopsis thaliana]
            gi|46394839|gb|AAS91582.1| DNA-directed polymerase kappa
            [Arabidopsis thaliana] gi|332194382|gb|AEE32503.1| DNA
            polymerase kappa subunit [Arabidopsis thaliana]
          Length = 671

 Score =  219 bits (558), Expect = 1e-54
 Identities = 116/189 (61%), Positives = 148/189 (78%), Gaps = 2/189 (1%)
 Frame = -1

Query: 1330 LCALFSHSSADFFLSVGLGLGGTDTPQAKSRKSISNERTFSATEDEAVLYRKIVELAEML 1151
            L ALFS SSADFFLSVGLGLGGT+TPQ +SRKSIS+ERTF+AT DE +LY K+ ELAEML
Sbjct: 311  LYALFSQSSADFFLSVGLGLGGTNTPQVRSRKSISSERTFAATGDERLLYSKLDELAEML 370

Query: 1150 SVDMKKEGLSGRTLTLKLKTASFEVRTRAVTLQNYIYSSEDILSHASKLLKAELPISIRL 971
            S DMKKEGL+ RTLTLKLKTASFE+R+RAV+LQ Y  SS+DIL HA+KLLKAELP+S+RL
Sbjct: 371  SHDMKKEGLTARTLTLKLKTASFEIRSRAVSLQRYTCSSDDILKHATKLLKAELPVSVRL 430

Query: 970  MGLRMSHFREDHDGFLADPTQKTLSSFFIPEDAKK--RNTDANDSLGADVDGSLLANNEE 797
            +GLRMS F E+     +DP+Q T++ F + +D+ +  ++ D NDS   D + + L+N+E 
Sbjct: 431  IGLRMSQFVEEIRN--SDPSQGTITKFIVQKDSSRQAQDLDDNDSFDLDANKNCLSNDES 488

Query: 796  AGLPIDNHE 770
              +   +HE
Sbjct: 489  GNVSFGSHE 497



 Score = 88.2 bits (217), Expect = 4e-15
 Identities = 43/88 (48%), Positives = 54/88 (61%), Gaps = 1/88 (1%)
 Frame = -3

Query: 458 ESSLWVDDYKCVLCGSEIPLIFAEERQEHSDFHLAERLQAEESDNYNDNRTLKHRVIDAA 279
           E   W+D YKCVLCG E+P  F EERQEHSDFHLA+RLQ EE+ + +     K R++   
Sbjct: 573 EPLYWIDGYKCVLCGIELPPSFVEERQEHSDFHLAQRLQNEETGSSSSTTPSKRRILGKE 632

Query: 278 SQGRGKKKQKL-SPSAGKHVPIDVFFSK 198
                 KKQK     + KH+PI  FF+K
Sbjct: 633 KVNSKPKKQKPDQKDSSKHIPIHAFFTK 660