BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00019443
         (1059 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002266054.1| PREDICTED: vinorine synthase-like [Vitis vin...   273   5e-71
ref|XP_002327870.1| predicted protein [Populus trichocarpa] gi|2...   262   1e-67
ref|XP_002305585.1| predicted protein [Populus trichocarpa] gi|2...   248   2e-63
ref|XP_002322397.1| predicted protein [Populus trichocarpa] gi|2...   247   4e-63
ref|XP_002329498.1| predicted protein [Populus trichocarpa] gi|2...   245   2e-62

>ref|XP_002266054.1| PREDICTED: vinorine synthase-like [Vitis vinifera]
          Length = 436

 Score =  273 bits (698), Expect = 5e-71
 Identities = 158/374 (42%), Positives = 217/374 (58%), Gaps = 24/374 (6%)
 Frame = +3

Query: 6    SHKLIKPSDPTPPHLKNYKLSFLDQIAAPIHVNIIFYY---------DQPLNIQHLEKSL 158
            S KL KPS PTP HL+ +KLS +DQ+   +     FYY         +     Q LEKSL
Sbjct: 7    SRKLRKPSSPTPSHLRTWKLSPIDQLVPQVKAAFAFYYPAHGTNSKVENVERCQRLEKSL 66

Query: 159  SQTLTIYYPLAGRFSKGDLFVDCNDQGVAFIEAKVLNLELDQLIHGKFEVGLLNNFVPWH 338
            S+TLT +YPLAG + K    V+CNDQG  ++EAKV + +L QL+  + E+  +   + + 
Sbjct: 67   SETLTQFYPLAGTYIKDSHSVECNDQGAEYLEAKV-DAQLTQLLSKRDEI--IEEVIHFA 123

Query: 339  INKVDQASTPLVSIQVNSFSCGGFAVCLRISHRIADGFTIASFLSAWSTASRVGIDGVVK 518
              K    ++PL++IQ   F CGG  + +RISH + DGFT + F+S W+TAS  GI+  + 
Sbjct: 124  SGKF---TSPLMTIQTTMFECGGLVIGVRISHTLVDGFTASKFISTWATASSEGINEAIL 180

Query: 519  PCFELPSHIPARILPTTQHSNSRKT--VTKIVTRRFVFDGAAIRTLK------------- 653
            PCF   S +PA+ LPT + S   K     K VT RFVFDGA I +LK             
Sbjct: 181  PCFNFASLLPAKDLPTLKPSPPPKIHGAEKFVTMRFVFDGANISSLKAKAKANSKTSTPG 240

Query: 654  ENIGCQATRVEVVTAVIWKAYINLSLAKHGNLKTSLLEHAVNLRGRTIPPMPSNTFANFI 833
             ++  Q +RVEVVTA+IW+A I +S  KHG L+TSL  H+ NLRG+ +P +P N   N  
Sbjct: 241  PSLKHQVSRVEVVTALIWRALIGVSQGKHGRLRTSLAVHSANLRGKIVPALPDNCCGNLY 300

Query: 834  ALALPRVSGNELCKMDLQSLVKLIQNAVKSTTNKSLHSEDFFTMVTSSFREVDYELNHDD 1013
             LA+ R   ++  KM+L SL  L+     +   ++L  ED F     SF EV+ EL  ++
Sbjct: 301  KLAVARFVVDD-SKMELPSLADLVGLVSGAIWTETLSQEDVFPTAIKSFNEVNEELGKEE 359

Query: 1014 VDYRMFTSWCRFPL 1055
            VD RMFTSWC+FPL
Sbjct: 360  VDVRMFTSWCKFPL 373


>ref|XP_002327870.1| predicted protein [Populus trichocarpa] gi|222837279|gb|EEE75658.1|
            predicted protein [Populus trichocarpa]
          Length = 438

 Score =  262 bits (669), Expect = 1e-67
 Identities = 145/374 (38%), Positives = 220/374 (58%), Gaps = 22/374 (5%)
 Frame = +3

Query: 3    LSHKLIKPSDPTPPHLKNYKLSFLDQIAAPIHVNIIFYYDQPLNIQ---------HLEKS 155
            +S K I PS  TPP L++ ++S LDQ+  P++V + ++Y    NIQ          LE+S
Sbjct: 6    VSRKFITPSSSTPPQLRSCEISALDQLLPPVYVGLTYFYTADENIQGAKHSERGKQLEES 65

Query: 156  LSQTLTIYYPLAGRFSKGDLFVDCNDQGVAFIEAKVLNLELDQLIHGKFEVGLLNNFVPW 335
            LS+ LT+YYP+AGR+ +  L +DCND+GV ++EA+V    L Q+++G+ +   LN F+P+
Sbjct: 66   LSKILTLYYPVAGRYDEEKLLIDCNDKGVEYLEAEVSG-RLSQILNGELQPEQLNQFLPY 124

Query: 336  HINKVDQASTPLVSIQVNSFSCGGFAVCLRISHRIADGFTIASFLSAWSTASRVGIDGVV 515
                V   ++PLVS+QV++F CGG AV LRISH+I D  T+ SF++ W+T SR+GID V 
Sbjct: 125  ---PVASPTSPLVSVQVSTFECGGLAVGLRISHKICDIATLTSFVNGWATTSRLGIDEVP 181

Query: 516  KPCFELPSHIPARILPTTQHSNSRKTVTKIVTRRFVFDGAAIRTLK----------ENIG 665
            +P F LPS  P R       +   K   ++VTR FVF  +A+  L+          E   
Sbjct: 182  RPSFALPSLFPTRETTQILKATPVKNGARVVTRTFVFSRSAVANLQVIARTRDDDLEGGK 241

Query: 666  CQATRVEVVTAVIWKAYINLSLAKHGNLKTSLLEHAVNLRGRTIPPMPSNTFANFIALAL 845
             Q +RV+VVTA++W+A I ++  KHG L+ S L H V +R R+  P+P N F N + +++
Sbjct: 242  GQPSRVQVVTAIVWRALIKVAKLKHGYLRPSALIHGVEMRKRSALPIPPNCFGNMLRVSM 301

Query: 846  PRVSGNELCKMDLQSLVKLIQNAVK---STTNKSLHSEDFFTMVTSSFREVDYELNHDDV 1016
             R   +E  KM L  LV  ++ A++   S    ++  +D       SF E + E +  ++
Sbjct: 302  ARFEADE-SKMGLPDLVIPVRQAIRDAVSDCRNAISYDDLLLGAAGSFSEWNEEASKGEI 360

Query: 1017 DYRMFTSWCRFPLY 1058
            D   FTSWC FP+Y
Sbjct: 361  DVYTFTSWCGFPIY 374


>ref|XP_002305585.1| predicted protein [Populus trichocarpa] gi|222848549|gb|EEE86096.1|
            predicted protein [Populus trichocarpa]
          Length = 433

 Score =  248 bits (632), Expect = 2e-63
 Identities = 155/367 (42%), Positives = 221/367 (60%), Gaps = 19/367 (5%)
 Frame = +3

Query: 3    LSHKLIKPSDPTPPHLKNYKLSFLDQIAAPIHVNIIFYYDQPL---NIQHLEKSLSQTLT 173
            +S KLIKPS  TPPHL+N  +SFLDQ+A  ++V  IFYY       NI+ LEKSLS+ LT
Sbjct: 6    ISRKLIKPSVQTPPHLQNLNISFLDQLAPSLNVPNIFYYTNNHHVNNIEDLEKSLSEILT 65

Query: 174  IYYPLAGRFSKGDLFVDCNDQGVAFIEAKVLNLELDQLIH--GKFEVGLLNNFVPWHINK 347
            ++YPLAGR+ + +L VDCND+GV F+EAKV  ++L Q+I       + LL++FVP     
Sbjct: 66   LFYPLAGRYIEDNLSVDCNDEGVEFLEAKVDGVDLTQIIQQDPNSNLDLLDHFVPC---V 122

Query: 348  VDQASTPLVSIQVNSFSCGGFAVCLRISHRIADGFTIASFLSAWSTASRV-GI-DGVVKP 521
             +  ++ L++IQ+N F CGG A+ L  SHRIAD  TI++F++AW+T  R  GI D V +P
Sbjct: 123  TESDTSRLLAIQINKFKCGGLAIGLLTSHRIADIPTISTFINAWATTFRERGISDQVRRP 182

Query: 522  CFELPSHIPARILPTTQHSNSRKTVTKIVTRRFVFDGAAIRTLKENIG--------CQAT 677
             F+ P   P R L       + K   KIVT+ FVF+  AI  LK  IG           +
Sbjct: 183  RFDCPFLFPQRDL-RLNFPPAPKVQPKIVTKVFVFNKEAIDKLKSKIGGGTDSGVKYHHS 241

Query: 678  RVEVVTAVIWKAYINLSLAKHGNLKTSLLEHAVNLRGRTIPPMPSNTFANFIALALPRVS 857
            R+EVVTA+IWKA I    A+HG L+ S +   + LRG+   P+P N   N     + R +
Sbjct: 242  RLEVVTALIWKALIGSVKAQHGQLRASSVRQIMTLRGKVSMPLPENACGNMYMPFISRFN 301

Query: 858  GN-ELCKMDLQSLVKLIQNAVK---STTNKSLHSEDFFTMVTSSFREVDYELNHDDVDYR 1025
             N E  +++L  +  L+++A +   S    +++S+D F+MVT   +E+  ELN  +VD  
Sbjct: 302  ANGESNRLELSDIASLLRDAKRKAISDCANAVNSDDVFSMVTKYHKELFEELNKGEVDSF 361

Query: 1026 MFTSWCR 1046
             F+SWCR
Sbjct: 362  KFSSWCR 368


>ref|XP_002322397.1| predicted protein [Populus trichocarpa] gi|222869393|gb|EEF06524.1|
            predicted protein [Populus trichocarpa]
          Length = 434

 Score =  247 bits (630), Expect = 4e-63
 Identities = 145/370 (39%), Positives = 211/370 (57%), Gaps = 18/370 (4%)
 Frame = +3

Query: 3    LSHKLIKPSDPTPPHLKNYKLSFLDQIAAPI-HVNIIFYY------DQPLNIQHLEKSLS 161
            LS KLI PS PTP HL+N K+S+LDQ  +P  ++  IFYY      +     + L+ SLS
Sbjct: 6    LSRKLITPSSPTPHHLQNLKISWLDQFLSPHNYLPFIFYYPSTGDENNEERSKQLQNSLS 65

Query: 162  QTLTIYYPLAGRFSKGDLFVDCNDQGVAFIEAKVLNLELDQLIHGKFEVGLLNNFVPWHI 341
            + LTI+YPLAGR+   +L +DCND+GV + EA+V       L  G+ E  L N+  P   
Sbjct: 66   EILTIFYPLAGRYIDNNLVIDCNDKGVEYTEAQVSGGLSLLLEGGELETELRNHLAP--- 122

Query: 342  NKVDQASTPLVSIQVNSFSCGGFAVCLRISHRIADGFTIASFLSAWSTASRVGIDGVVK- 518
            + V   ++PLV IQ N F  GG A+ L ++HR+AD +T+ +F+  W+TA ++G+D V+  
Sbjct: 123  HPVQPDNSPLVLIQFNMFESGGVAIGLCVTHRVADAYTVFTFVRTWATACKLGVDEVLHA 182

Query: 519  PCFELPSHIPARILPTTQHSNSRKTVTKIVT-RRFVFDGAAIRTLKENIGC------QAT 677
            P F+LPS  P+R   ++ +    +   KIV  +RFVF GAA+  LK  +        Q T
Sbjct: 183  PSFQLPSFFPSRDTISSPNRFIGRNHHKIVVMKRFVFTGAALSKLKAVVSASVNGSHQPT 242

Query: 678  RVEVVTAVIWKAYINLSLAKHGNLKTSLLEHAVNLRGRTIPPMPSNTFANFIALALPRVS 857
            RVEVVTAVIWK    ++ AKHG L+ SLL H  N+RG+   P+P N+  NFI +AL   +
Sbjct: 243  RVEVVTAVIWKTLTMVAQAKHGRLRPSLLSHTFNMRGKIAMPIPDNSCGNFINVALSHFT 302

Query: 858  GNELCKMDLQSLVKLIQNAVKSTTN---KSLHSEDFFTMVTSSFREVDYELNHDDVDYRM 1028
             ++  K+ L   V  + N +K+  +   +    ++ F M      E        ++D  M
Sbjct: 303  ADDETKVQLHDFVDRVYNGIKNMVSDCARVSSDDELFVMAEKIRIETIKAFTRSEMDLYM 362

Query: 1029 FTSWCRFPLY 1058
            F SWCR P+Y
Sbjct: 363  FNSWCRMPVY 372


>ref|XP_002329498.1| predicted protein [Populus trichocarpa] gi|222870178|gb|EEF07309.1|
            predicted protein [Populus trichocarpa]
          Length = 435

 Score =  245 bits (625), Expect = 2e-62
 Identities = 146/373 (39%), Positives = 217/373 (58%), Gaps = 22/373 (5%)
 Frame = +3

Query: 6    SHKLIKPSDPTPPHLKNYKLSFLDQIAAPIHVNIIFYYD---QPLNIQH------LEKSL 158
            S KLI PS PTPPHL+N +++  DQ+A   +V  IFYY    +   +++      L+KSL
Sbjct: 7    SRKLITPSSPTPPHLQNTRVTCFDQLAPFFYVPTIFYYPVEGEEYAVKNAEKSLLLQKSL 66

Query: 159  SQTLTIYYPLAGRFSKGDLFVDCNDQGVAFIEAKVLNLELDQLIHGKFEVGLLN-NFVPW 335
            S+ LT+Y+P AGR+++ +L ++CND+GV ++EAKV    L Q +       L+  NF P 
Sbjct: 67   SEILTLYHPFAGRYARDNLSIECNDKGVEYLEAKVYG-SLSQFLQRTSPNHLVQQNFHPH 125

Query: 336  HINKVDQASTPLVSIQVNSFSCGGFAVCLRISHRIADGFTIASFLSAWSTASRVGIDGVV 515
            +       S+PL ++Q N F CGG A+ + ++H+I DGFT+ + ++ W+TA R+G++ V 
Sbjct: 126  Y-------SSPL-AVQFNEFECGGVAIGISMTHKIGDGFTMFTLINNWATACRIGVNKVH 177

Query: 516  KPCFELPSHIPARILPTTQHSN-SRKTVTKIVTRRFVFDGAAIRTLKENIGC-------- 668
             P FEL    P R     Q+    R    KIVT++FVFDG AI  LK             
Sbjct: 178  PPSFELGIIFPPREASRVQNVVLGRAPTNKIVTKKFVFDGEAISNLKVAASASSSQFNRH 237

Query: 669  QATRVEVVTAVIWKAYINLSLAKHGNLKTSLLEHAVNLRGRTIPPMPSNTFANFIALALP 848
            Q TRV+VVTA+IW A+I +  A+ G  + S+L+  VNLRG+T   +P N+  NFI+ A+ 
Sbjct: 238  QLTRVKVVTALIWSAFIRVDQARDGRRRPSMLKVPVNLRGKTNIKIPENSCGNFISWAVT 297

Query: 849  RVSGNELCKMDLQSLVKLIQNAVKSTTN---KSLHSEDFFTMVTSSFREVDYELNHDDVD 1019
            +   N+  K+ L  LV  I +A++ T +   K+ + ED F MV   F++V   L   + D
Sbjct: 298  QYLPNDEIKIQLHELVSRIHDAIEKTVSNYEKASNGEDLFFMVNEDFQKVSQALKESEAD 357

Query: 1020 YRMFTSWCRFPLY 1058
              MF+ W RFPLY
Sbjct: 358  VYMFSCWSRFPLY 370