BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00019426
         (1017 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002280430.2| PREDICTED: phosphoinositide phospholipase C ...   298   e-111
ref|XP_002533650.1| 1-phosphatidylinositol-4,5-bisphosphate phos...   296   e-111
ref|NP_001234203.1| PI-phospholipase C PLC1 [Solanum lycopersicu...   291   e-109
ref|XP_003616636.1| Phosphoinositide phospholipase C [Medicago t...   288   e-109
ref|XP_002269949.1| PREDICTED: phosphoinositide phospholipase C ...   290   e-108

>ref|XP_002280430.2| PREDICTED: phosphoinositide phospholipase C 2-like [Vitis vinifera]
          Length = 594

 Score =  298 bits (764), Expect(2) = e-111
 Identities = 147/247 (59%), Positives = 173/247 (70%), Gaps = 7/247 (2%)
 Frame = +3

Query: 12  KKSSEEKVWGKEISDIRKKLEAVHEVNFLHNIL*YVLIFVYI*EVMCNQKELDGGEAYGR 191
           K  SE+K WGKEISD++ +LE                          ++ E + GE    
Sbjct: 276 KYPSEDKTWGKEISDLKDELEPF------------------------DKDEPEEGEDINE 311

Query: 192 SD-------LKHNVDPEYKHLIAFHARKRKGGFKDWLHDDPNKVTRISLREQNLETAVID 350
            D       L+ +  PEY+ LIA  A K+KGG K+W+  DP+KV R+SL EQ LE A+I 
Sbjct: 312 EDMEDWDHKLQQSAAPEYRRLIAIQAGKQKGGIKEWIRLDPDKVRRLSLSEQELENAIIT 371

Query: 351 HGADVIRFTRRNLLRIFPKGSRVDSSNYNPFIGWMHGAQMVAFNMQSHGRSLWLMQGLFR 530
           HG D+IRFT+RNLLR+FPK  RVDSSNYNP IGWMHGAQMVAFNMQ + R LWLMQG+FR
Sbjct: 372 HGQDIIRFTQRNLLRVFPKVMRVDSSNYNPLIGWMHGAQMVAFNMQGYDRPLWLMQGMFR 431

Query: 531 ANGGCGYVKKPDFLLKVGPNNEVFDPKRRLPVKQTLKLKIYMGEGWHLDFDDTHFDIYSP 710
           ANGGCGYVKKPD LLKVGP+++VFDPK  LPVK TLK+K+YMGEGWHLDF  THFDIYSP
Sbjct: 432 ANGGCGYVKKPDLLLKVGPHDKVFDPKANLPVKTTLKVKVYMGEGWHLDFHHTHFDIYSP 491

Query: 711 PDFYVKV 731
           PDFY KV
Sbjct: 492 PDFYTKV 498



 Score =  132 bits (332), Expect(2) = e-111
 Identities = 60/77 (77%), Positives = 69/77 (89%)
 Frame = +2

Query: 785  FYAMQVGIAGVPSDSIMKKTKTIQDNWIPTWNEEFEFPLTVPELALLRIEVHEYDLSDND 964
            FY  +VGIAGVP+DS+MKKTK ++D+W PTWNEEFEFPLT PELALLRIEVHEYD+S+ D
Sbjct: 494  FYT-KVGIAGVPADSLMKKTKAVEDDWTPTWNEEFEFPLTAPELALLRIEVHEYDMSEKD 552

Query: 965  DFAGQTCLPVPELKTGI 1015
            DF GQTCLPV EL+TGI
Sbjct: 553  DFGGQTCLPVSELRTGI 569


>ref|XP_002533650.1| 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase, putative
           [Ricinus communis] gi|223526463|gb|EEF28738.1|
           1-phosphatidylinositol-4,5-bisphosphate
           phosphodiesterase, putative [Ricinus communis]
          Length = 594

 Score =  296 bits (759), Expect(2) = e-111
 Identities = 143/242 (59%), Positives = 173/242 (71%)
 Frame = +3

Query: 6   SGKKSSEEKVWGKEISDIRKKLEAVHEVNFLHNIL*YVLIFVYI*EVMCNQKELDGGEAY 185
           SGK  S+E+ WGKEI D++     V + N L                  +  + +     
Sbjct: 274 SGKPGSDEETWGKEIPDLKGHFR-VDDKNELDE----------------DNNDDEDAAPD 316

Query: 186 GRSDLKHNVDPEYKHLIAFHARKRKGGFKDWLHDDPNKVTRISLREQNLETAVIDHGADV 365
           G +  + N+ PEYKHLIA HA K KGG ++ L  DP+KV R+SL EQ LE A   HG ++
Sbjct: 317 GDNKSQQNIAPEYKHLIAIHAGKPKGGIEECLKVDPDKVRRLSLSEQQLEKAAETHGKEI 376

Query: 366 IRFTRRNLLRIFPKGSRVDSSNYNPFIGWMHGAQMVAFNMQSHGRSLWLMQGLFRANGGC 545
           +RFT+RN+LR++PKG RVDSSNYNP IGWMHGAQMVAFNMQ HGRSLWLMQG+FRANGGC
Sbjct: 377 VRFTQRNILRVYPKGIRVDSSNYNPLIGWMHGAQMVAFNMQGHGRSLWLMQGMFRANGGC 436

Query: 546 GYVKKPDFLLKVGPNNEVFDPKRRLPVKQTLKLKIYMGEGWHLDFDDTHFDIYSPPDFYV 725
           GYVKKPDFLLK GP+ EVFDP+ +LPVK TLK+K+YMGEGW+ DFD THFD YSPPDFY 
Sbjct: 437 GYVKKPDFLLKSGPHGEVFDPRAKLPVKTTLKVKVYMGEGWYYDFDRTHFDAYSPPDFYA 496

Query: 726 KV 731
           +V
Sbjct: 497 RV 498



 Score =  134 bits (337), Expect(2) = e-111
 Identities = 61/77 (79%), Positives = 71/77 (92%)
 Frame = +2

Query: 785  FYAMQVGIAGVPSDSIMKKTKTIQDNWIPTWNEEFEFPLTVPELALLRIEVHEYDLSDND 964
            FYA +VGIAGVP+D+IMKKTKT++DNWIP WNEEFEFPLTVPELALLR+EVHEYD+S+ D
Sbjct: 494  FYA-RVGIAGVPADTIMKKTKTLEDNWIPVWNEEFEFPLTVPELALLRVEVHEYDMSEKD 552

Query: 965  DFAGQTCLPVPELKTGI 1015
            DF GQ+CLPV EL+ GI
Sbjct: 553  DFGGQSCLPVSELRKGI 569


>ref|NP_001234203.1| PI-phospholipase C PLC1 [Solanum lycopersicum]
           gi|158827652|gb|ABW81003.1| PI-phospholipase C PLC1
           [Solanum lycopersicum]
          Length = 601

 Score =  291 bits (746), Expect(2) = e-109
 Identities = 142/241 (58%), Positives = 167/241 (69%)
 Frame = +3

Query: 9   GKKSSEEKVWGKEISDIRKKLEAVHEVNFLHNIL*YVLIFVYI*EVMCNQKELDGGEAYG 188
           GKKSSEEK WG EISD+ +K+ A  E                     C   E D    + 
Sbjct: 276 GKKSSEEKAWGAEISDLSQKMMAFSENKDNGE---------------CQDDEADSH--HE 318

Query: 189 RSDLKHNVDPEYKHLIAFHARKRKGGFKDWLHDDPNKVTRISLREQNLETAVIDHGADVI 368
             +++ N+ PEYKHLIA  A K KG   +WL  DP KV R+SL E+ L    ++HG D++
Sbjct: 319 NPNIQQNIAPEYKHLIAIQAGKSKGPTSEWLTVDPIKVKRVSLNEEKLINVALNHGQDLV 378

Query: 369 RFTRRNLLRIFPKGSRVDSSNYNPFIGWMHGAQMVAFNMQSHGRSLWLMQGLFRANGGCG 548
           RFT+RNLLR++PKG RVDSSNYNP IGWMHG QMVAFN+Q HGR LWLMQG+FRANGGCG
Sbjct: 379 RFTQRNLLRVYPKGMRVDSSNYNPLIGWMHGTQMVAFNIQGHGRPLWLMQGMFRANGGCG 438

Query: 549 YVKKPDFLLKVGPNNEVFDPKRRLPVKQTLKLKIYMGEGWHLDFDDTHFDIYSPPDFYVK 728
           YVKKP+ LLK   NNEV DPKR L VK TLK+K+YMG+GWHLDF  THFD YSPPDFYVK
Sbjct: 439 YVKKPELLLKTDANNEVHDPKRLLSVKTTLKVKVYMGKGWHLDFKRTHFDAYSPPDFYVK 498

Query: 729 V 731
           +
Sbjct: 499 I 499



 Score =  131 bits (329), Expect(2) = e-109
 Identities = 57/76 (75%), Positives = 70/76 (92%)
 Frame = +2

Query: 788  YAMQVGIAGVPSDSIMKKTKTIQDNWIPTWNEEFEFPLTVPELALLRIEVHEYDLSDNDD 967
            + +++GIAGVP+DS +KKTK I+DNWIPTWN+EFEFPLTVPELALLR+EVHEYD+S+ DD
Sbjct: 495  FYVKIGIAGVPADSRVKKTKAIEDNWIPTWNDEFEFPLTVPELALLRVEVHEYDMSEIDD 554

Query: 968  FAGQTCLPVPELKTGI 1015
            F GQTC+PV EL+TGI
Sbjct: 555  FGGQTCIPVSELRTGI 570


>ref|XP_003616636.1| Phosphoinositide phospholipase C [Medicago truncatula]
           gi|355517971|gb|AES99594.1| Phosphoinositide
           phospholipase C [Medicago truncatula]
          Length = 589

 Score =  288 bits (736), Expect(2) = e-109
 Identities = 140/241 (58%), Positives = 168/241 (69%)
 Frame = +3

Query: 9   GKKSSEEKVWGKEISDIRKKLEAVHEVNFLHNIL*YVLIFVYI*EVMCNQKELDGGEAYG 188
           GK S +++ WG EI   +  L + ++ N L              E   +++EL   +   
Sbjct: 270 GKASGDDEAWGTEIPSFKLGLLSDYKGNSLDK------------EYFHDEEELSESDKS- 316

Query: 189 RSDLKHNVDPEYKHLIAFHARKRKGGFKDWLHDDPNKVTRISLREQNLETAVIDHGADVI 368
                HNV PEYK LIA HA K KGG   WL  DP K  RISL EQ LE A I HG +V+
Sbjct: 317 ----HHNVAPEYKGLIAIHAGKSKGGIDAWLKIDPEKARRISLSEQQLEKAAITHGKEVV 372

Query: 369 RFTRRNLLRIFPKGSRVDSSNYNPFIGWMHGAQMVAFNMQSHGRSLWLMQGLFRANGGCG 548
           RFT++N+LR+FPKG+R+DSSNYNP IGW+HGAQMVAFNMQ +GRSLWLM G+FRANGGCG
Sbjct: 373 RFTQKNILRVFPKGTRIDSSNYNPLIGWIHGAQMVAFNMQGYGRSLWLMHGMFRANGGCG 432

Query: 549 YVKKPDFLLKVGPNNEVFDPKRRLPVKQTLKLKIYMGEGWHLDFDDTHFDIYSPPDFYVK 728
           YVKKPDFLL  GP+NEVFDPK +LPVK TLK+ +YMGEGW+ DF  THFD YSPPDFY +
Sbjct: 433 YVKKPDFLLTTGPDNEVFDPKAKLPVKTTLKVTVYMGEGWYYDFKHTHFDQYSPPDFYAR 492

Query: 729 V 731
           V
Sbjct: 493 V 493



 Score =  135 bits (339), Expect(2) = e-109
 Identities = 62/77 (80%), Positives = 71/77 (92%)
 Frame = +2

Query: 785  FYAMQVGIAGVPSDSIMKKTKTIQDNWIPTWNEEFEFPLTVPELALLRIEVHEYDLSDND 964
            FYA +VGIAGVPSDSIMKKT+ I+DNW+PTWNE FEFPLTVPELALLRIEVHEYD+S+ D
Sbjct: 489  FYA-RVGIAGVPSDSIMKKTRAIEDNWLPTWNEVFEFPLTVPELALLRIEVHEYDMSEKD 547

Query: 965  DFAGQTCLPVPELKTGI 1015
            DF GQTCLPV EL++G+
Sbjct: 548  DFGGQTCLPVSELRSGV 564


>ref|XP_002269949.1| PREDICTED: phosphoinositide phospholipase C 2 isoform 1 [Vitis
           vinifera] gi|147828132|emb|CAN64084.1| hypothetical
           protein VITISV_006935 [Vitis vinifera]
          Length = 593

 Score =  290 bits (742), Expect(2) = e-108
 Identities = 138/241 (57%), Positives = 173/241 (71%)
 Frame = +3

Query: 9   GKKSSEEKVWGKEISDIRKKLEAVHEVNFLHNIL*YVLIFVYI*EVMCNQKELDGGEAYG 188
           GK+S++E+ WGKE+S+++    AV E N                E   ++++LD G+   
Sbjct: 274 GKESADEEAWGKEVSNLKPSTVAVDESNLEE-------------EENIDEEDLDEGDPKS 320

Query: 189 RSDLKHNVDPEYKHLIAFHARKRKGGFKDWLHDDPNKVTRISLREQNLETAVIDHGADVI 368
           +     N  PEYK LIA HA K KGG  +WL  DP+KV R+SL EQ LE AV+ HG +++
Sbjct: 321 QQ----NAAPEYKRLIAIHAGKVKGGLTEWLRVDPDKVRRLSLSEQELEKAVLTHGKEIV 376

Query: 369 RFTRRNLLRIFPKGSRVDSSNYNPFIGWMHGAQMVAFNMQSHGRSLWLMQGLFRANGGCG 548
           RFT+RNLLR++PKG R DSSNYNP IGWMHGAQMVAFNMQ +GRSLW+M G+F+ANGGCG
Sbjct: 377 RFTQRNLLRVYPKGIRFDSSNYNPVIGWMHGAQMVAFNMQGYGRSLWIMHGMFKANGGCG 436

Query: 549 YVKKPDFLLKVGPNNEVFDPKRRLPVKQTLKLKIYMGEGWHLDFDDTHFDIYSPPDFYVK 728
           YVKKPDFLL  G ++ VFDPK +LPVK TLK+ +YMGEGW+ DF  THFD YSPPDFY +
Sbjct: 437 YVKKPDFLLNNGSHDGVFDPKAKLPVKTTLKVTVYMGEGWYFDFRHTHFDAYSPPDFYAR 496

Query: 729 V 731
           V
Sbjct: 497 V 497



 Score =  130 bits (326), Expect(2) = e-108
 Identities = 59/77 (76%), Positives = 69/77 (89%)
 Frame = +2

Query: 785  FYAMQVGIAGVPSDSIMKKTKTIQDNWIPTWNEEFEFPLTVPELALLRIEVHEYDLSDND 964
            FYA +VGIAGVP+D+IMKKTKT++DNW P W+E FEFPLTVPELALLR+EVHEYD+S+ D
Sbjct: 493  FYA-RVGIAGVPADTIMKKTKTLEDNWTPNWDEHFEFPLTVPELALLRVEVHEYDMSEKD 551

Query: 965  DFAGQTCLPVPELKTGI 1015
            DF GQTCLPV EL+ GI
Sbjct: 552  DFGGQTCLPVSELRRGI 568