BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00019357
         (1361 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002279724.1| PREDICTED: importin subunit alpha-2 [Vitis v...   611   e-172
ref|XP_002525173.1| Importin alpha-2 subunit, putative [Ricinus ...   608   e-171
ref|XP_002323457.1| predicted protein [Populus trichocarpa] gi|2...   601   e-169
emb|CAN61727.1| hypothetical protein VITISV_032422 [Vitis vinifera]   587   e-165
ref|XP_004142168.1| PREDICTED: importin subunit alpha-2-like [Cu...   567   e-159

>ref|XP_002279724.1| PREDICTED: importin subunit alpha-2 [Vitis vinifera]
            gi|297741569|emb|CBI32701.3| unnamed protein product
            [Vitis vinifera]
          Length = 517

 Score =  611 bits (1575), Expect = e-172
 Identities = 314/415 (75%), Positives = 358/415 (86%)
 Frame = +2

Query: 116  MADESPASHKRDPIKSSVGHVAAQRRKQHAVSIGKERREALVRTKRLCRLGVSSDTNVPT 295
            MAD+  ASH+RDPIKSSVG+VAA RR+Q A+++GKERREALVRTKRLCR+G+S D++VP 
Sbjct: 1    MADDGLASHRRDPIKSSVGNVAANRRRQQAITVGKERREALVRTKRLCRVGISGDSDVPV 60

Query: 296  ESDMMIDEEQSILEAQTSTAVEELKLAVGYQGKGALQKKVAALRKLRHLLSRSEFPPIEA 475
            + DMMIDEEQSILE QTS+AVEELK  V YQGKGA+QK+V+ALR LR LLSRSEFPPIEA
Sbjct: 61   DGDMMIDEEQSILETQTSSAVEELKSTVIYQGKGAMQKRVSALRDLRRLLSRSEFPPIEA 120

Query: 476  AIRSGAISLLVQCLSFGSLDEQLLEAAWCLTNIAAGKPEETQXXXXXXXXXVAHIGEKSS 655
            A+++GA+S+LVQCLSFGS DEQLLEAAWCLTNIAAGKPEET+         +AH+GEKSS
Sbjct: 121  ALKAGAVSVLVQCLSFGSPDEQLLEAAWCLTNIAAGKPEETKALVPALPLLIAHLGEKSS 180

Query: 656  LPVAEQCAWALGNVAGEGVELRNVLLVQGALPPLARMMLPDKGSAVRTAAWALSNLIKGP 835
             PVAEQCAWALGNVAGEG ELRN+LL QGALPPLARMM+P+KGS VRTAAWALSNLIKGP
Sbjct: 181  PPVAEQCAWALGNVAGEGEELRNILLSQGALPPLARMMVPNKGSTVRTAAWALSNLIKGP 240

Query: 836  EPKAATELIRINGVLDAILRHLRKSDEELATEXXXXXXXXXXXXXXXTSILVKSDVLQLL 1015
            +PKAA+ELI+I+GVLDAILRHLRK DEELATE               TSILVKS++LQLL
Sbjct: 241  DPKAASELIQIDGVLDAILRHLRKGDEELATEVAWVVVYLSALSDTATSILVKSNLLQLL 300

Query: 1016 AERLTTSNSLQLLIPVLRSLGNLVAGDAHTTNAVLVPGHDITDNVVAALVRCLKSEHRVL 1195
             ERL TSNSLQLLIPVLRSLGNL AG +H T  +LV GH++TDN +AALV+CL SEHRVL
Sbjct: 301  VERLATSNSLQLLIPVLRSLGNLGAGASHAT-CILVVGHEMTDNFIAALVKCLGSEHRVL 359

Query: 1196 KKEASWVLSNIAAGSVEHKQLIYSSEAVSLMLHLFTTAPFDIRKEVAYVLGNLCV 1360
            KKEA+WVLSNIAAGS+EHK+LIYSSEAV L+L L +TAPFDIRKEVAY LGNLCV
Sbjct: 360  KKEAAWVLSNIAAGSIEHKKLIYSSEAVPLLLRLLSTAPFDIRKEVAYALGNLCV 414


>ref|XP_002525173.1| Importin alpha-2 subunit, putative [Ricinus communis]
            gi|223535470|gb|EEF37139.1| Importin alpha-2 subunit,
            putative [Ricinus communis]
          Length = 517

 Score =  608 bits (1567), Expect = e-171
 Identities = 314/415 (75%), Positives = 361/415 (86%)
 Frame = +2

Query: 116  MADESPASHKRDPIKSSVGHVAAQRRKQHAVSIGKERREALVRTKRLCRLGVSSDTNVPT 295
            MA+E   S KR+PIKSSVG+VAAQ+RKQ+AV++GKERRE+LVR KRLCR+G S D+    
Sbjct: 1    MAEEGLTSLKREPIKSSVGNVAAQKRKQNAVAVGKERRESLVRAKRLCRVGTSGDS---- 56

Query: 296  ESDMMIDEEQSILEAQTSTAVEELKLAVGYQGKGALQKKVAALRKLRHLLSRSEFPPIEA 475
            +S+M+IDEEQSIL+AQTS+AVEELK AV +QGKGA+QKKV ALR+LR LLSRSEFPPIEA
Sbjct: 57   DSEMIIDEEQSILDAQTSSAVEELKSAVAFQGKGAMQKKVGALRELRRLLSRSEFPPIEA 116

Query: 476  AIRSGAISLLVQCLSFGSLDEQLLEAAWCLTNIAAGKPEETQXXXXXXXXXVAHIGEKSS 655
            AI++GAI LLVQCLSFGS DEQLLEAAWCLTNIAAGKPEET+         +AH+GE+SS
Sbjct: 117  AIKAGAIILLVQCLSFGSPDEQLLEAAWCLTNIAAGKPEETKALLPALPLLIAHLGERSS 176

Query: 656  LPVAEQCAWALGNVAGEGVELRNVLLVQGALPPLARMMLPDKGSAVRTAAWALSNLIKGP 835
             PVAEQCAWALGNVAGEG +LRNVLL QGALPPLARMMLP++GS VRTAAWALSNLIKGP
Sbjct: 177  SPVAEQCAWALGNVAGEGEDLRNVLLSQGALPPLARMMLPNRGSTVRTAAWALSNLIKGP 236

Query: 836  EPKAATELIRINGVLDAILRHLRKSDEELATEXXXXXXXXXXXXXXXTSILVKSDVLQLL 1015
            +PKAATELIR++GVLDAI+RHLRK+DEELATE               TS+LVKSDVLQLL
Sbjct: 237  DPKAATELIRVDGVLDAIVRHLRKADEELATEVAWAVVYLSALSNVATSMLVKSDVLQLL 296

Query: 1016 AERLTTSNSLQLLIPVLRSLGNLVAGDAHTTNAVLVPGHDITDNVVAALVRCLKSEHRVL 1195
             +RL TSNSLQLLIPVLRSLGNL+AGD+HT +AVL+PG +ITD+++A LV+CLKSEHRVL
Sbjct: 297  IQRLATSNSLQLLIPVLRSLGNLIAGDSHTISAVLLPGIEITDSIIAVLVKCLKSEHRVL 356

Query: 1196 KKEASWVLSNIAAGSVEHKQLIYSSEAVSLMLHLFTTAPFDIRKEVAYVLGNLCV 1360
            KKEA+WVLSNIAAGSVEHKQLIY SEAV L+L L +TAPFD+RKEVAYVLGNLCV
Sbjct: 357  KKEAAWVLSNIAAGSVEHKQLIYCSEAVPLLLRLLSTAPFDLRKEVAYVLGNLCV 411


>ref|XP_002323457.1| predicted protein [Populus trichocarpa] gi|222868087|gb|EEF05218.1|
            predicted protein [Populus trichocarpa]
          Length = 522

 Score =  601 bits (1550), Expect = e-169
 Identities = 310/416 (74%), Positives = 357/416 (85%), Gaps = 1/416 (0%)
 Frame = +2

Query: 116  MADESPASHKRDPIKSSVGHVAAQRRKQHAVSIGKERREALVRTKRLCRLGVS-SDTNVP 292
            MA++    H+RDPIKSSVG+VAA RR+Q+AV++GKERRE+LVR KRLCR+G S  D+++ 
Sbjct: 1    MAEDGLNPHRRDPIKSSVGNVAAHRRRQNAVAVGKERRESLVRAKRLCRVGPSCDDSDIS 60

Query: 293  TESDMMIDEEQSILEAQTSTAVEELKLAVGYQGKGALQKKVAALRKLRHLLSRSEFPPIE 472
             E+DM+IDEEQSILEAQTS+AVEELK AV +QGKGA+QK V ALR++R LLS+SEFPP+E
Sbjct: 61   IENDMIIDEEQSILEAQTSSAVEELKSAVSFQGKGAMQKLVGALREMRRLLSKSEFPPVE 120

Query: 473  AAIRSGAISLLVQCLSFGSLDEQLLEAAWCLTNIAAGKPEETQXXXXXXXXXVAHIGEKS 652
            AAI++GAI LLVQCLSFGS DEQLLEAAWCLTNIAAGKPEET+         +AH+GEKS
Sbjct: 121  AAIKAGAIPLLVQCLSFGSPDEQLLEAAWCLTNIAAGKPEETKALLPALPLLIAHLGEKS 180

Query: 653  SLPVAEQCAWALGNVAGEGVELRNVLLVQGALPPLARMMLPDKGSAVRTAAWALSNLIKG 832
            SLPVAEQCAWALGNVAGEG ELR+VLL QGALPPLARMMLP+KGS VRTAAWALSNLIKG
Sbjct: 181  SLPVAEQCAWALGNVAGEGEELRSVLLCQGALPPLARMMLPNKGSTVRTAAWALSNLIKG 240

Query: 833  PEPKAATELIRINGVLDAILRHLRKSDEELATEXXXXXXXXXXXXXXXTSILVKSDVLQL 1012
            P+ KAATELIR++GVLDAILRHLRK+DEELATE               TS+LVKSD LQL
Sbjct: 241  PDHKAATELIRVDGVLDAILRHLRKADEELATEVAWVVVYLSALSNVATSMLVKSDALQL 300

Query: 1013 LAERLTTSNSLQLLIPVLRSLGNLVAGDAHTTNAVLVPGHDITDNVVAALVRCLKSEHRV 1192
            L +RL +SNSLQLLIPVLRSLGN++AGD+HT  AVL+PG +ITDNV+  LV+CLKSEHRV
Sbjct: 301  LVQRLASSNSLQLLIPVLRSLGNVIAGDSHTIVAVLLPGCEITDNVIEVLVKCLKSEHRV 360

Query: 1193 LKKEASWVLSNIAAGSVEHKQLIYSSEAVSLMLHLFTTAPFDIRKEVAYVLGNLCV 1360
            LKKEA+WVLSNIAAGS+EHKQLIY SEA  L+L L +TAPFDIRKEVAYVLGNLCV
Sbjct: 361  LKKEAAWVLSNIAAGSIEHKQLIYYSEAAPLLLRLLSTAPFDIRKEVAYVLGNLCV 416


>emb|CAN61727.1| hypothetical protein VITISV_032422 [Vitis vinifera]
          Length = 530

 Score =  587 bits (1512), Expect = e-165
 Identities = 311/432 (71%), Positives = 353/432 (81%), Gaps = 17/432 (3%)
 Frame = +2

Query: 116  MADESPASHKRDPIKSS-----------------VGHVAAQRRKQHAVSIGKERREALVR 244
            MAD+  ASH+RDPIKSS                  G+VAA RR+Q A+++GKERREALVR
Sbjct: 1    MADDGLASHRRDPIKSSGIFTFILTIVSLSLPSPFGNVAANRRRQQAITVGKERREALVR 60

Query: 245  TKRLCRLGVSSDTNVPTESDMMIDEEQSILEAQTSTAVEELKLAVGYQGKGALQKKVAAL 424
            TKRLCR+G+S D++VP + DMMIDEEQSILE QTS+AVEELK  V YQGKGA+QK+V+AL
Sbjct: 61   TKRLCRVGISGDSDVPVDGDMMIDEEQSILETQTSSAVEELKSTVIYQGKGAMQKRVSAL 120

Query: 425  RKLRHLLSRSEFPPIEAAIRSGAISLLVQCLSFGSLDEQLLEAAWCLTNIAAGKPEETQX 604
            R LR LLSRSEFPPIEAA+++GA+S+LVQCLSFGS DEQLLEAAWCLTNIAAGKPEET+ 
Sbjct: 121  RDLRRLLSRSEFPPIEAALKAGAVSVLVQCLSFGSPDEQLLEAAWCLTNIAAGKPEETKA 180

Query: 605  XXXXXXXXVAHIGEKSSLPVAEQCAWALGNVAGEGVELRNVLLVQGALPPLARMMLPDKG 784
                    +AH+GEKSS PVAEQCAWALGNVAGEG ELRN+LL QGALPPLARMM+P+KG
Sbjct: 181  LLPALPLLIAHLGEKSSPPVAEQCAWALGNVAGEGEELRNILLSQGALPPLARMMVPNKG 240

Query: 785  SAVRTAAWALSNLIKGPEPKAATELIRINGVLDAILRHLRKSDEELATEXXXXXXXXXXX 964
            S VRTAAWALSNLIKGP+PKAA+ELI+I+GVLDAILRHLRK DEELATE           
Sbjct: 241  STVRTAAWALSNLIKGPDPKAASELIQIDGVLDAILRHLRKGDEELATEVAWVVVYLSAL 300

Query: 965  XXXXTSILVKSDVLQLLAERLTTSNSLQLLIPVLRSLGNLVAGDAHTTNAVLVPGHDITD 1144
                TSILVKS++LQLL ERL TSNSLQLLIPVLRSLGNL AG +H T   LV GH++T 
Sbjct: 301  SDTATSILVKSBLLQLLVERLATSNSLQLLIPVLRSLGNLGAGASHAT-CXLVVGHEMT- 358

Query: 1145 NVVAALVRCLKSEHRVLKKEASWVLSNIAAGSVEHKQLIYSSEAVSLMLHLFTTAPFDIR 1324
               AALV+CL SEHRVLKKEA+WVLSNIAAGS+EHK+LIYSSEAV L+L L +TAPFDIR
Sbjct: 359  ---AALVKCLGSEHRVLKKEAAWVLSNIAAGSIEHKKLIYSSEAVPLLLRLLSTAPFDIR 415

Query: 1325 KEVAYVLGNLCV 1360
            KEVAY LGNLCV
Sbjct: 416  KEVAYALGNLCV 427


>ref|XP_004142168.1| PREDICTED: importin subunit alpha-2-like [Cucumis sativus]
          Length = 518

 Score =  567 bits (1461), Expect = e-159
 Identities = 287/415 (69%), Positives = 346/415 (83%)
 Frame = +2

Query: 116  MADESPASHKRDPIKSSVGHVAAQRRKQHAVSIGKERREALVRTKRLCRLGVSSDTNVPT 295
            MAD S  S +RD IKSSVG VAA RR+QHA+++GKERR+ LVR KR CR+G+        
Sbjct: 1    MADSSLPSPRRDSIKSSVGSVAANRRRQHAIAVGKERRDLLVRAKRFCRIGIGDGDGDGV 60

Query: 296  ESDMMIDEEQSILEAQTSTAVEELKLAVGYQGKGALQKKVAALRKLRHLLSRSEFPPIEA 475
            +++M++DEE SILE QTS+AV+ELK AV YQGKGA+QK++ ALR+LR LLSRSEFPP+E 
Sbjct: 61   DNEMIMDEELSILEVQTSSAVDELKSAVAYQGKGAMQKRIHALRELRRLLSRSEFPPVET 120

Query: 476  AIRSGAISLLVQCLSFGSLDEQLLEAAWCLTNIAAGKPEETQXXXXXXXXXVAHIGEKSS 655
            A+++GA+SLLVQCLSFGS DEQLLEAAWCLTNI AGKPEET+         +AH+GE+SS
Sbjct: 121  ALKAGAVSLLVQCLSFGSPDEQLLEAAWCLTNIGAGKPEETKSLLPAIPLLIAHLGERSS 180

Query: 656  LPVAEQCAWALGNVAGEGVELRNVLLVQGALPPLARMMLPDKGSAVRTAAWALSNLIKGP 835
            L VAEQCAWALGNVAGE  ELRN+LL QGAL PLARM+LP+KGS+V+TAAWALSNLIKGP
Sbjct: 181  LLVAEQCAWALGNVAGEEKELRNILLSQGALLPLARMLLPNKGSSVKTAAWALSNLIKGP 240

Query: 836  EPKAATELIRINGVLDAILRHLRKSDEELATEXXXXXXXXXXXXXXXTSILVKSDVLQLL 1015
            + +AATELIRI+GVLDAI+RHLRK+D+ELATE                SILVKS+V+QLL
Sbjct: 241  DSRAATELIRIDGVLDAIIRHLRKADDELATEVAWVIVYLSALSDVAISILVKSEVVQLL 300

Query: 1016 AERLTTSNSLQLLIPVLRSLGNLVAGDAHTTNAVLVPGHDITDNVVAALVRCLKSEHRVL 1195
             ERL+TSNSLQLLIPVLRSLGNLVA D+HT +A+L+PG +IT +V+  L++CLKSEHRVL
Sbjct: 301  VERLSTSNSLQLLIPVLRSLGNLVAVDSHTISAILIPGSEITGSVLEVLIKCLKSEHRVL 360

Query: 1196 KKEASWVLSNIAAGSVEHKQLIYSSEAVSLMLHLFTTAPFDIRKEVAYVLGNLCV 1360
            KKEASWVLSNIAAGS+EHKQLIY+S+ V L++ L ++APFD+RKEVAYVLGNLCV
Sbjct: 361  KKEASWVLSNIAAGSMEHKQLIYTSDVVPLLIRLLSSAPFDVRKEVAYVLGNLCV 415