BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00019298
         (1395 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002272045.1| PREDICTED: diacylglycerol kinase 1-like [Vit...   421   e-115
emb|CAN71651.1| hypothetical protein VITISV_017443 [Vitis vinifera]   419   e-115
ref|XP_003630377.1| Diacylglycerol kinase eta [Medicago truncatu...   378   e-102
ref|XP_003525142.1| PREDICTED: diacylglycerol kinase 1-like [Gly...   374   e-101
ref|XP_002306287.1| predicted protein [Populus trichocarpa] gi|2...   349   1e-93

>ref|XP_002272045.1| PREDICTED: diacylglycerol kinase 1-like [Vitis vinifera]
          Length = 714

 Score =  421 bits (1082), Expect = e-115
 Identities = 216/368 (58%), Positives = 251/368 (68%), Gaps = 15/368 (4%)
 Frame = -3

Query: 1060 MIDL-NILYRLLITSDSYAPIV-GWLLTASVGFLVVIYAFLKWQRNTSLNWVXXXXXXXX 887
            MIDL N L RLL T D+Y P V GWL+T S G L +IYAFLKWQR TSLNW+        
Sbjct: 1    MIDLSNSLLRLLTTPDAYGPSVFGWLITGSFGILAIIYAFLKWQRRTSLNWIKAAAKAKK 60

Query: 886  XXXXXXKVPLSRHTWVEDFSHGKQPSTCCVCLTSVVSSHNTGIKAMTYFPVQRCSVCGVS 707
                   VPLS H+W E+F++  QP TC VCLTS+VS    G KA    PV RCSVCGV+
Sbjct: 61   KVWKRLNVPLSHHSWTEEFAYDVQPCTCSVCLTSLVSPQTLGAKATPQNPVHRCSVCGVA 120

Query: 706  AHYNCYQFATKDCKCVAQAGFSHVLHHWSERWTTTDENSEMSAFCSYCDESCGVPFLDAS 527
            AH++C +FA KDCKCVAQAGF HV H WSERW   D+N EMSAFC YCDE CGVP LDA 
Sbjct: 121  AHFHCSKFAAKDCKCVAQAGFGHVQHQWSERWIDMDDNHEMSAFCFYCDEPCGVPLLDA- 179

Query: 526  PTWHCLWCQRLIHVKCHGKLSEESGNVCDMGSLRRVILSPLCVKEVEDEKNRGGMLNSIT 347
            PTWHCLWCQRLIHVKCH K+SEESG+VCD+G+LRR+ILSP+CVKEV++E  R  ML+SIT
Sbjct: 180  PTWHCLWCQRLIHVKCHAKMSEESGDVCDLGALRRLILSPICVKEVDEEITRSRMLSSIT 239

Query: 346  EEFIASSVRGQIRRRRHRNKHGSGRSINSKLQDNSAANTALQYVLNGIA----------- 200
             + IASSV G+IRRRRHRNKHGS RS+N KLQ+ S ANTALQYVLNG+A           
Sbjct: 240  GDIIASSVCGEIRRRRHRNKHGSVRSVNGKLQNTSPANTALQYVLNGLASLKQSSNQNKD 299

Query: 199  --XXXXXXXXXXXGTQNDILHKNQEIVSQGRFRRYEXXXXXXXXXXXLVFINTKSGARNG 26
                          TQN    K + I   G+ ++Y            LVFINTKSGA+ G
Sbjct: 300  PILKRGGRVLSGKDTQNGWQKKGRSI-PYGQVKKYTLVDLPQDARPLLVFINTKSGAQYG 358

Query: 25   PALKRRLN 2
            P+L+RRLN
Sbjct: 359  PSLRRRLN 366


>emb|CAN71651.1| hypothetical protein VITISV_017443 [Vitis vinifera]
          Length = 376

 Score =  419 bits (1078), Expect = e-115
 Identities = 214/371 (57%), Positives = 253/371 (68%), Gaps = 18/371 (4%)
 Frame = -3

Query: 1060 MIDL-NILYRLLITSDSYAPIV-GWLLTASVGFLVVIYAFLKWQRNTSLNWVXXXXXXXX 887
            MIDL N L RLL T D+Y P V GWL+T S G L +IYAFLKWQR TSLNW+        
Sbjct: 1    MIDLSNSLLRLLTTPDAYGPSVFGWLITGSFGILAIIYAFLKWQRRTSLNWIKAAAKAKK 60

Query: 886  XXXXXXKVPLSRHTWVEDFSHGKQPSTCCVCLTSVVSSHNTGIKAMTYFPVQRCSVCGVS 707
                   VPLS H+W E+F++  QP TC VCLTS+VS    G KA    PV RCSVCGV+
Sbjct: 61   KVWKRLNVPLSHHSWTEEFAYDVQPCTCSVCLTSLVSPQTLGAKATPQNPVHRCSVCGVA 120

Query: 706  AHYNCYQFATKDCKCVAQAGFSHVLHHWSERWTTTDENSEMSAFCSYCDESCGVPFLDAS 527
            AH++C +FA KDCKCVAQAGF HV H WSERW   D+N EMSAFC YCDE CGVP LDA 
Sbjct: 121  AHFHCSKFAAKDCKCVAQAGFGHVQHQWSERWIDMDDNHEMSAFCFYCDEPCGVPLLDA- 179

Query: 526  PTWHCLWCQRLIHVKCHGKLSEESGNVCDMGSLRRVILSPLCVKEVEDEKNRGGMLNSIT 347
            PTWHCLWCQRLIHVKCH K+SEESG+VCD+G+LRR+ILSP+CVKEV++E  R  ML+SIT
Sbjct: 180  PTWHCLWCQRLIHVKCHAKMSEESGDVCDLGALRRLILSPICVKEVDEEITRSRMLSSIT 239

Query: 346  EEFIASSVRGQIRRRRHRNKHGSGRSINSKLQDNSAANTALQYVLNGIAXXXXXXXXXXX 167
             + IASSV G+IRRRRHRNKHGS RS+N KLQ+ S ANTALQYVLNG+A           
Sbjct: 240  GDIIASSVCGEIRRRRHRNKHGSVRSVNGKLQNTSPANTALQYVLNGLA----SLKQSSN 295

Query: 166  GTQNDILHKNQEIVS----------------QGRFRRYEXXXXXXXXXXXLVFINTKSGA 35
              ++ IL +   ++S                 G+ ++Y            LVFINTKSGA
Sbjct: 296  QNKDPILKRGGRVLSGKDTQNGWQKKGRSXPYGQVKKYTLVDLPQDARPLLVFINTKSGA 355

Query: 34   RNGPALKRRLN 2
            + GP+L+RRLN
Sbjct: 356  QYGPSLRRRLN 366


>ref|XP_003630377.1| Diacylglycerol kinase eta [Medicago truncatula]
            gi|355524399|gb|AET04853.1| Diacylglycerol kinase eta
            [Medicago truncatula]
          Length = 729

 Score =  378 bits (971), Expect = e-102
 Identities = 191/359 (53%), Positives = 235/359 (65%), Gaps = 3/359 (0%)
 Frame = -3

Query: 1069 SFTMIDLNILYRLLITSD--SYAPIVGWLLTASVGFLVVIYAFLKWQRNTSLNWVXXXXX 896
            SF M DL I+    +TS   S A I GWL+T S G + VIYA LKWQR  SLNW+     
Sbjct: 2    SFVMFDLGIVILRFLTSPDASIASIFGWLITGSFGLVAVIYAVLKWQRRASLNWIKAAAR 61

Query: 895  XXXXXXXXXKVPLSRHTWVEDFSHGKQPSTCCVCLTSVVSSHNTGIKAMTYFPVQRCSVC 716
                     KVPLS H WVEDF++G+QPSTCC CLTS+  S N G  A    P+ RCSVC
Sbjct: 62   EKKKVWKKFKVPLSDHLWVEDFTYGEQPSTCCFCLTSLWPSQNIGATASPRTPLHRCSVC 121

Query: 715  GVSAHYNCYQFATKDCKCVAQAGFSHVLHHWSERWTTTDENSEMSAFCSYCDESCGVPFL 536
            GV+AH+ C  +A KDCKCVAQAGFSH+ HHWSERW   D++ EMSAFC YCDE+CGVPF+
Sbjct: 122  GVAAHFICSPYAAKDCKCVAQAGFSHIRHHWSERWVNVDDHHEMSAFCFYCDEACGVPFV 181

Query: 535  DASPTWHCLWCQRLIHVKCHGKLSEESGNVCDMGSLRRVILSPLCVKEVEDEKNRGGMLN 356
             +SPTWHC WCQRLIHVKCH  L+ +SG+ CD+GSLR +ILSPLCVKEV DE  +GG L+
Sbjct: 182  KSSPTWHCRWCQRLIHVKCHNNLTRDSGDFCDLGSLRPIILSPLCVKEV-DENQKGGKLS 240

Query: 355  SITEEFIASSVRGQIRRRRHRNKHGSGRSINSKLQDNSAAN-TALQYVLNGIAXXXXXXX 179
            SI    I SSVRGQIR+RR+ NK+G G   N K + ++ A+ T L+YVLNG+        
Sbjct: 241  SI----ITSSVRGQIRKRRNHNKNGGGCHTNGKSRGSAVADPTLLEYVLNGLKRKKYNDG 296

Query: 178  XXXXGTQNDILHKNQEIVSQGRFRRYEXXXXXXXXXXXLVFINTKSGARNGPALKRRLN 2
                 T N  +  +   V+  + ++Y            LVFINT+SG + GP+L RRLN
Sbjct: 297  KLFDPTNNGRVSGDGLNVTPSQIKKYTLVDLPKDARPLLVFINTRSGGQLGPSLHRRLN 355


>ref|XP_003525142.1| PREDICTED: diacylglycerol kinase 1-like [Glycine max]
          Length = 704

 Score =  374 bits (960), Expect = e-101
 Identities = 191/359 (53%), Positives = 232/359 (64%), Gaps = 3/359 (0%)
 Frame = -3

Query: 1069 SFTMIDLNILYRLLITSD--SYAPIVGWLLTASVGFLVVIYAFLKWQRNTSLNWVXXXXX 896
            S TMIDL I +  L+TS   S A I GWL+T S G + VIYA LKWQR +SLNW+     
Sbjct: 2    SLTMIDLGISFLRLVTSPDASSASIFGWLITGSFGLMAVIYAVLKWQRRSSLNWIKAAAR 61

Query: 895  XXXXXXXXXKVPLSRHTWVEDFSHGKQPSTCCVCLTSVVSSHNTGIKAMTYFPVQRCSVC 716
                     KVPLS H WVEDF++ +QPSTCC CLTS+  S N G  A    P+ RCSVC
Sbjct: 62   EKKKVWKKFKVPLSEHLWVEDFTYREQPSTCCFCLTSLWPSQNLGTTASPRTPLHRCSVC 121

Query: 715  GVSAHYNCYQFATKDCKCVAQAGFSHVLHHWSERWTTTDENSEMSAFCSYCDESCGVPFL 536
            GV+AH+ C QFA KDCKCVAQAGF H+ HHWSERW   DEN EMSAFC YCDE CGVPF+
Sbjct: 122  GVAAHFLCSQFAAKDCKCVAQAGFGHIRHHWSERWVDVDENHEMSAFCFYCDEPCGVPFV 181

Query: 535  DASPTWHCLWCQRLIHVKCHGKLSEESGNVCDMGSLRRVILSPLCVKEVEDEKNRGGMLN 356
             ASPTW C WCQRLIHVKCH KL+ +SG+ CD+G LRR+ILSPLCVK+V+++K +GG L+
Sbjct: 182  KASPTWDCRWCQRLIHVKCHNKLTRDSGDFCDLGPLRRIILSPLCVKQVDEDK-QGGRLS 240

Query: 355  SITEEFIASSVRGQIRRRRHRNKHGSGRSINSKLQDNSAAN-TALQYVLNGIAXXXXXXX 179
            SI    I SSV GQIR+RR+RNK   G + N K   +S  + T L+YVLNG+        
Sbjct: 241  SI----ITSSVNGQIRKRRNRNKSLGGYNANGKSDGSSITDATLLEYVLNGLHWNKFGDE 296

Query: 178  XXXXGTQNDILHKNQEIVSQGRFRRYEXXXXXXXXXXXLVFINTKSGARNGPALKRRLN 2
                   N  +  N    +  + ++Y            LVFIN +SG + GP+L RRLN
Sbjct: 297  KLFDLVNNGRVLGNGLTATPNQIKKYTLVGLPQDASPLLVFINARSGGQLGPSLHRRLN 355


>ref|XP_002306287.1| predicted protein [Populus trichocarpa] gi|222855736|gb|EEE93283.1|
            predicted protein [Populus trichocarpa]
          Length = 712

 Score =  349 bits (895), Expect = 1e-93
 Identities = 181/368 (49%), Positives = 225/368 (61%), Gaps = 15/368 (4%)
 Frame = -3

Query: 1060 MIDLNI-LYRLLITSDSY--APIVGWLLTASVGFLVVIYAFLKWQRNTSLNWVXXXXXXX 890
            M+ L I L R+L +S  Y     + WL+TA++GFLV++Y  + W +  S  WV       
Sbjct: 1    MMGLGISLVRVLASSYDYYSGSFIAWLITAALGFLVIVYRLINWHKRASQKWVKAAAKAK 60

Query: 889  XXXXXXXKVPLSRHTWVEDFSHG-KQPSTCCVCLTSVVSSHNTGIKAMTYFPVQRCSVCG 713
                    VP S H W+ED ++G KQPS CCVCLTS+V  +N    ++    V RC VCG
Sbjct: 61   KQVWKRLNVPRSSHLWIEDLAYGQKQPSACCVCLTSLVLPYNGCSASLRSDFVHRCVVCG 120

Query: 712  VSAHYNCYQFATKDCKCVAQAGFSHVLHHWSERWTTTDENSEMSAFCSYCDESCGVPFLD 533
             +AH+NC +FA KDCKC+AQAG SHV HHWSERW   DEN EMS FCSYCDE CGV F+D
Sbjct: 121  AAAHFNCSEFAVKDCKCIAQAGSSHVRHHWSERWFNMDENPEMSVFCSYCDEPCGVTFID 180

Query: 532  ASPTWHCLWCQRLIHVKCHGKLSEESGNVCDMGSLRRVILSPLCVKEVEDEKNRGGMLNS 353
            ASPTWHCLWCQRLIHV+C  K+S+ESG+VCD+G LRR+ILSPLCVKEV+     G  L+ 
Sbjct: 181  ASPTWHCLWCQRLIHVRCQAKMSKESGDVCDLGPLRRIILSPLCVKEVDG----GNSLSL 236

Query: 352  ITEEFIASSVRGQIRRR-RHRNKHGSGRSINSKLQDNSAANTALQYVLNGIA-------- 200
            ITEE IASSVRGQ+RR+ R R KHG  R +N KLQ  + AN    +VLNG A        
Sbjct: 237  ITEEIIASSVRGQMRRKQRSRGKHGDDRPVNGKLQGATTANRNSDFVLNGFAGLNRSSSE 296

Query: 199  --XXXXXXXXXXXGTQNDILHKNQEIVSQGRFRRYEXXXXXXXXXXXLVFINTKSGARNG 26
                          T N ++H         + ++Y            LVFIN+KSG + G
Sbjct: 297  TDFHCLKKNGRIKYTLNGLMHDKGGTAICEQVKKYALADLAQDARPLLVFINSKSGGQLG 356

Query: 25   PALKRRLN 2
            P L+R LN
Sbjct: 357  PILRRTLN 364