BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00019223
         (1468 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002524579.1| ubiquitin-protein ligase, putative [Ricinus ...   473   e-131
ref|XP_003528813.1| PREDICTED: F-box/LRR-repeat protein 12-like ...   447   e-123
ref|NP_566312.1| F-box/LRR-repeat protein 12 [Arabidopsis thalia...   435   e-119
ref|XP_002882535.1| F-box family protein [Arabidopsis lyrata sub...   426   e-117
ref|XP_002323784.1| predicted protein [Populus trichocarpa] gi|2...   422   e-115

>ref|XP_002524579.1| ubiquitin-protein ligase, putative [Ricinus communis]
            gi|223536132|gb|EEF37787.1| ubiquitin-protein ligase,
            putative [Ricinus communis]
          Length = 389

 Score =  473 bits (1217), Expect = e-131
 Identities = 228/382 (59%), Positives = 281/382 (73%)
 Frame = +1

Query: 262  DDTTSIMHLPDDCLYIIFKRLESGFDRKSFGLTCRRWLHIENTSRXXXXXXXXXXXXXXS 441
            D  TSIMHL DDCL IIF+ L+   DR+SFGLTCRR L I+N +R              +
Sbjct: 7    DGPTSIMHLSDDCLSIIFQWLDCNSDRESFGLTCRRLLDIQNINRRSLQFQCSFTIFNLT 66

Query: 442  PLSRTSTTIGPFQLSRLLNRFQQLELLSLSGCIDLPDSGLTQLQYYGSKLQRLHLDCCFG 621
             L + S  I  F + RLL RFQ L  LSLSGC DLPDS L  LQ+YGS+L  LHLDCCFG
Sbjct: 67   SLPQRSLFINSFHIHRLLTRFQHLHFLSLSGCTDLPDSALIPLQFYGSRLHSLHLDCCFG 126

Query: 622  ITDDGLSMVALGCHSLTVISLYRCNVSSIGIETLAKSCLALEEVNLSYCALITDTGIRAL 801
            +TD+GLS++  GC  LTVISLYRCN++ IG+ETLA  C AL+++NLSYC L++D G+R++
Sbjct: 127  LTDNGLSLITSGCPYLTVISLYRCNITDIGLETLANGCSALKQINLSYCPLVSDCGLRSI 186

Query: 802  SQNCRQLRAVRISSCRNITGNGFHGCPQTLVYLEADSCKLVPEGITEIVSGGGLEYLNIS 981
            SQ C QL+AV+IS CR I+G GF GC  TL Y++A+SC L P+G+  IVSGGGLEYLN+S
Sbjct: 187  SQACCQLQAVKISCCREISGVGFTGCSPTLAYIDAESCNLDPKGVMGIVSGGGLEYLNVS 246

Query: 982  NLYWAIPGNGLTTIGAGLCKGLKILDFRLCRTVSEESIIAIARGCPLLQEWNLALCHEVK 1161
             + W+I G+GL  IG+G    LKIL+ R+CRTV +ES  AIA+GCPLLQEWNLALCH V+
Sbjct: 247  GISWSIKGDGLAAIGSGFAARLKILNLRMCRTVGDESATAIAKGCPLLQEWNLALCHGVQ 306

Query: 1162 LPGWESIAVSCHNLERLHVNRCRYLCDQGLQALREGCRKLSVLYFTRCRQISDPAIMLFK 1341
            + GWESI   C+ LE+LHVNRCR LCD+GLQALREGC+ LSVLY  +  ++S  AI LFK
Sbjct: 307  ISGWESIGFGCNRLEKLHVNRCRNLCDRGLQALREGCKMLSVLYLNKSCRVSSNAIELFK 366

Query: 1342 CLRGDVEIKEEEVMCIAPGWAF 1407
              RG+VEI+EEEVMCI P   F
Sbjct: 367  LYRGNVEIREEEVMCIGPARTF 388


>ref|XP_003528813.1| PREDICTED: F-box/LRR-repeat protein 12-like [Glycine max]
          Length = 388

 Score =  447 bits (1150), Expect = e-123
 Identities = 219/389 (56%), Positives = 281/389 (72%), Gaps = 1/389 (0%)
 Frame = +1

Query: 244  MDTVARDDTTSIMHLPDDCLYIIFKRLESGFDRKSFGLTCRRWLHIENTSRXXXXXXXXX 423
            M  ++R+D TSIMHLPDDCL IIF  L+S  DR SFGLTCRRWLH+++ +R         
Sbjct: 1    MGDLSRNDCTSIMHLPDDCLVIIFHGLDSRIDRDSFGLTCRRWLHVQDFNRQSLQFECSS 60

Query: 424  XXXXXSPLSRTSTTIGPFQLSRLLNRFQQLELLSLSGCIDLPDSGLTQLQYYGSKLQRLH 603
                    S     I  F L RLL RFQ L+ LSLS C +L DSGLT+L  YGS LQ+L+
Sbjct: 61   TALRPLSSSTKGFDIHTFHLHRLLRRFQHLKSLSLSNCSELSDSGLTRLLSYGSNLQKLN 120

Query: 604  LDCCFGITDDGLSMVALGCHSLTVISLYRC-NVSSIGIETLAKSCLALEEVNLSYCALIT 780
            LDCC  +TD GLS+VA GC SL  ISLYRC  ++  G++TLA +CL+++ VNLSYC+ I+
Sbjct: 121  LDCCLKVTDYGLSLVASGCPSLMSISLYRCPGITDKGLDTLASACLSMKYVNLSYCSQIS 180

Query: 781  DTGIRALSQNCRQLRAVRISSCRNITGNGFHGCPQTLVYLEADSCKLVPEGITEIVSGGG 960
            D G++A++  CRQL+A+ IS C  ++G GF GC +TL Y+EA+SCKL  EG+  IVSGGG
Sbjct: 181  DNGLKAITHWCRQLQAINISHCEGLSGVGFEGCSKTLAYVEAESCKLKQEGVMGIVSGGG 240

Query: 961  LEYLNISNLYWAIPGNGLTTIGAGLCKGLKILDFRLCRTVSEESIIAIARGCPLLQEWNL 1140
            +EYL++S L W++ G+ L  IG   C  LKIL+FRLCRTVS+ SI+AIA+GCPLL+EWNL
Sbjct: 241  IEYLDVSCLSWSVLGDPLPGIGFASC--LKILNFRLCRTVSDTSIVAIAKGCPLLEEWNL 298

Query: 1141 ALCHEVKLPGWESIAVSCHNLERLHVNRCRYLCDQGLQALREGCRKLSVLYFTRCRQISD 1320
            ALCHEV+ PGW ++ + C NL+RLHVNRCR LCD GLQALREGC+ LS+LY   C +++ 
Sbjct: 299  ALCHEVREPGWRTVGLYCRNLKRLHVNRCRNLCDNGLQALREGCKNLSILYLNGCVRLTS 358

Query: 1321 PAIMLFKCLRGDVEIKEEEVMCIAPGWAF 1407
             A+ LFKC R +V IK+ E+MCI P W F
Sbjct: 359  VALELFKCQRANVCIKDIEIMCIKPYWEF 387


>ref|NP_566312.1| F-box/LRR-repeat protein 12 [Arabidopsis thaliana]
            gi|30680350|ref|NP_850534.1| F-box/LRR-repeat protein 12
            [Arabidopsis thaliana]
            gi|75207381|sp|Q9SRR1.1|FBL12_ARATH RecName:
            Full=F-box/LRR-repeat protein 12
            gi|6041850|gb|AAF02159.1|AC009853_19 unknown protein
            [Arabidopsis thaliana] gi|26452863|dbj|BAC43510.1|
            unknown protein [Arabidopsis thaliana]
            gi|30793809|gb|AAP40357.1| putative F-box protein family,
            AtFBL12 [Arabidopsis thaliana]
            gi|332641038|gb|AEE74559.1| F-box/LRR-repeat protein 12
            [Arabidopsis thaliana] gi|332641039|gb|AEE74560.1|
            F-box/LRR-repeat protein 12 [Arabidopsis thaliana]
          Length = 395

 Score =  435 bits (1119), Expect = e-119
 Identities = 215/389 (55%), Positives = 280/389 (71%), Gaps = 3/389 (0%)
 Frame = +1

Query: 244  MDTVARDDT---TSIMHLPDDCLYIIFKRLESGFDRKSFGLTCRRWLHIENTSRXXXXXX 414
            M+ V+  D    TSI+HLPDDCL  IF+RL+S  D  SFGLTC RWL+I+N SR      
Sbjct: 1    MEDVSESDNNVETSIIHLPDDCLSFIFQRLDSVADHDSFGLTCHRWLNIQNISRRSLQFQ 60

Query: 415  XXXXXXXXSPLSRTSTTIGPFQLSRLLNRFQQLELLSLSGCIDLPDSGLTQLQYYGSKLQ 594
                    S LS+T+  +    L RLL RFQ LE LSLSGC  L DS L  L+Y G++L 
Sbjct: 61   CSFSVLNPSSLSQTNPDVSSHHLHRLLTRFQWLEHLSLSGCTVLNDSSLDSLRYPGARLH 120

Query: 595  RLHLDCCFGITDDGLSMVALGCHSLTVISLYRCNVSSIGIETLAKSCLALEEVNLSYCAL 774
             L+LDCCFGI+DDG+S +A  C +L+V+SLYRCN+S IG+ETLA++ L+L+ VNLSYC L
Sbjct: 121  TLYLDCCFGISDDGISTIASFCPNLSVVSLYRCNISDIGLETLARASLSLKCVNLSYCPL 180

Query: 775  ITDTGIRALSQNCRQLRAVRISSCRNITGNGFHGCPQTLVYLEADSCKLVPEGITEIVSG 954
            ++D GI+ALSQ C QL +V+IS+C++ITG GF GC  TL Y++ADSC+L P+GIT I+SG
Sbjct: 181  VSDFGIKALSQACLQLESVKISNCKSITGVGFSGCSPTLGYVDADSCQLEPKGITGIISG 240

Query: 955  GGLEYLNISNLYWAIPGNGLTTIGAGLCKGLKILDFRLCRTVSEESIIAIARGCPLLQEW 1134
            GG+E+LNIS +   I  +GL  IG+G+   L+IL+ R+CRTV +ESI AIA+GCPLLQEW
Sbjct: 241  GGIEFLNISGVSCYIRKDGLVPIGSGIASKLRILNLRMCRTVGDESIEAIAKGCPLLQEW 300

Query: 1135 NLALCHEVKLPGWESIAVSCHNLERLHVNRCRYLCDQGLQALREGCRKLSVLYFTRCRQI 1314
            NLALCHEVK+ GWE++   C NL++LHVNRCR LCDQGL ALR GC  L +LY     ++
Sbjct: 301  NLALCHEVKISGWEAVGKWCRNLKKLHVNRCRNLCDQGLLALRCGCMNLQILYMNGNARL 360

Query: 1315 SDPAIMLFKCLRGDVEIKEEEVMCIAPGW 1401
            +  AI +F+  R D+ ++ EE+M I P W
Sbjct: 361  TPTAIEMFRLHRADITLRTEEMMVIGPDW 389


>ref|XP_002882535.1| F-box family protein [Arabidopsis lyrata subsp. lyrata]
            gi|297328375|gb|EFH58794.1| F-box family protein
            [Arabidopsis lyrata subsp. lyrata]
          Length = 394

 Score =  426 bits (1095), Expect = e-117
 Identities = 207/388 (53%), Positives = 279/388 (71%), Gaps = 2/388 (0%)
 Frame = +1

Query: 244  MDTVARDDT--TSIMHLPDDCLYIIFKRLESGFDRKSFGLTCRRWLHIENTSRXXXXXXX 417
            M+ V+  D   TSI+HLPDDCL  IF+RL++  D  SFGLTC RWL+I+N SR       
Sbjct: 1    MEDVSESDNVETSIIHLPDDCLSFIFQRLDNVADHDSFGLTCHRWLNIQNISRRSLQFQC 60

Query: 418  XXXXXXXSPLSRTSTTIGPFQLSRLLNRFQQLELLSLSGCIDLPDSGLTQLQYYGSKLQR 597
                   + LS+T+  +  + L RLL RFQ LE LSLSGC  L DS L  L+Y G++L  
Sbjct: 61   SFTVLNPASLSQTNPDVNSYHLHRLLTRFQWLEHLSLSGCTVLNDSSLASLRYPGARLHS 120

Query: 598  LHLDCCFGITDDGLSMVALGCHSLTVISLYRCNVSSIGIETLAKSCLALEEVNLSYCALI 777
            L+LDCCFGI+DDG+S +A  C +L V+SLYRCN+S IG+ETLA++ L+L+ VNLSYC L+
Sbjct: 121  LYLDCCFGISDDGISTIASFCPNLRVVSLYRCNISDIGLETLARASLSLKCVNLSYCPLV 180

Query: 778  TDTGIRALSQNCRQLRAVRISSCRNITGNGFHGCPQTLVYLEADSCKLVPEGITEIVSGG 957
            +D GI+ALSQ C QL +V++S+C++ITG GF+GC  TL Y++A+SC+L P+GI  I+SGG
Sbjct: 181  SDLGIKALSQACLQLESVKVSNCKSITGVGFNGCSPTLGYVDAESCQLEPKGIMGIISGG 240

Query: 958  GLEYLNISNLYWAIPGNGLTTIGAGLCKGLKILDFRLCRTVSEESIIAIARGCPLLQEWN 1137
            G+E+LNIS +   I  +GL  IG+G+   L++L+ R+CRTV + SI AIA+GCPLLQEWN
Sbjct: 241  GIEFLNISGVSCYIRKDGLVPIGSGIASKLRMLNLRMCRTVGDASIEAIAKGCPLLQEWN 300

Query: 1138 LALCHEVKLPGWESIAVSCHNLERLHVNRCRYLCDQGLQALREGCRKLSVLYFTRCRQIS 1317
            LALCHEVK+ GW+++   C NL++LHVNRCR LCDQGL ALR GC  L +LY     +++
Sbjct: 301  LALCHEVKVSGWKAVGKWCRNLKKLHVNRCRNLCDQGLLALRCGCMNLQILYMNGNARLT 360

Query: 1318 DPAIMLFKCLRGDVEIKEEEVMCIAPGW 1401
              AI +F+  R D+ ++ EE+M I P W
Sbjct: 361  PTAIEMFRLHRADITLRTEEMMVIGPDW 388


>ref|XP_002323784.1| predicted protein [Populus trichocarpa] gi|222866786|gb|EEF03917.1|
            predicted protein [Populus trichocarpa]
          Length = 374

 Score =  422 bits (1085), Expect = e-115
 Identities = 208/351 (59%), Positives = 253/351 (72%)
 Frame = +1

Query: 343  KSFGLTCRRWLHIENTSRXXXXXXXXXXXXXXSPLSRTSTTIGPFQLSRLLNRFQQLELL 522
            +SFGLTC RWL+I+NT R              S LS+    +  + L RLL RFQ L  L
Sbjct: 22   ESFGLTCHRWLNIQNTHRRSLQFHSSLALPNVSSLSQRGLVVSAYHLHRLLTRFQHLHYL 81

Query: 523  SLSGCIDLPDSGLTQLQYYGSKLQRLHLDCCFGITDDGLSMVALGCHSLTVISLYRCNVS 702
            SLSGC +LPDS LT LQ Y SKL  L+LDCCFGITD+GLS+VA GC SL  ISLYRCN++
Sbjct: 82   SLSGCSELPDSCLTFLQSYPSKLLHLNLDCCFGITDNGLSLVAAGCSSLEAISLYRCNIT 141

Query: 703  SIGIETLAKSCLALEEVNLSYCALITDTGIRALSQNCRQLRAVRISSCRNITGNGFHGCP 882
              G+ETLA  C AL+ +NLSYC+L++D G+RALSQ+C  L AV+IS C  + G GF GC 
Sbjct: 142  DAGLETLANGCSALKHINLSYCSLVSDGGLRALSQSCCHLEAVKISHCSGVNGTGFKGCS 201

Query: 883  QTLVYLEADSCKLVPEGITEIVSGGGLEYLNISNLYWAIPGNGLTTIGAGLCKGLKILDF 1062
             TL +++ADSC L PEGI  IVSGGGLEYLN+S + W   G+ L  IGAG    LKIL+ 
Sbjct: 202  PTLTHIDADSCNLDPEGIMGIVSGGGLEYLNVSRVNWWRSGDTLAVIGAGFATRLKILNL 261

Query: 1063 RLCRTVSEESIIAIARGCPLLQEWNLALCHEVKLPGWESIAVSCHNLERLHVNRCRYLCD 1242
             LCRTV +ESI AIARGCPLLQEWN+ALCH V++ GW+SI ++C+ LE+LHVNRCR LCD
Sbjct: 262  WLCRTVGDESIAAIARGCPLLQEWNVALCHGVRIAGWQSIGINCNKLEKLHVNRCRNLCD 321

Query: 1243 QGLQALREGCRKLSVLYFTRCRQISDPAIMLFKCLRGDVEIKEEEVMCIAP 1395
             GLQALREGC++L VLY  R  ++S  AI LFK  RG+VEI +EE+M I P
Sbjct: 322  LGLQALREGCKRLLVLYIGRPWKLSATAIELFKLCRGNVEISKEEIMHIGP 372