BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00019204
         (2017 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002270470.1| PREDICTED: ubiquitin carboxyl-terminal hydro...   626   e-177
ref|XP_003632213.1| PREDICTED: ubiquitin carboxyl-terminal hydro...   605   e-170
ref|XP_002515876.1| Ubiquitin carboxyl-terminal hydrolase, putat...   586   e-165
emb|CBI24406.3| unnamed protein product [Vitis vinifera]              559   e-157
ref|XP_004140175.1| PREDICTED: ubiquitin carboxyl-terminal hydro...   555   e-155

>ref|XP_002270470.1| PREDICTED: ubiquitin carboxyl-terminal hydrolase 8-like [Vitis
            vinifera]
          Length = 961

 Score =  626 bits (1615), Expect = e-177
 Identities = 348/601 (57%), Positives = 433/601 (72%), Gaps = 21/601 (3%)
 Frame = +2

Query: 275  QLYLVQHSWWNEAHESVFSRDVTGVSYLTTASLSKIEDFWEI--DDFTSSEIVMEMKRDD 448
            ++Y V + W NEAH +    +  G+ Y  TAS S      EI   DF  S+IV+ ++R++
Sbjct: 97   KVYFVPYRWLNEAHGTSLGEN-EGILY--TASSSTCVGSMEIMSSDF-DSQIVLNLRREE 152

Query: 449  DL---QSSEEGVSC-EYALLPDWMFLRALKWHND---VHKD-GTIVTAKGTLVDLISLKM 604
            D    +++EEGVS  EYALL + M+LRALKWHND     KD G+I+ A+    D+  L++
Sbjct: 153  DNGKNEAAEEGVSGQEYALLSESMWLRALKWHNDSNAAEKDVGSILAAEVYTQDVFPLQI 212

Query: 605  RCFLG-KMNFLVAKISQKDNEVGMFKRACDIFCVEGDQFRIRDFSGQITQFTVNDRNIPA 781
            R  +    N L+ KISQKDN + +++RA  IF VE +   I DFSGQ T   +NDR   A
Sbjct: 213  RLSVSWGTNSLLVKISQKDNPIEIYERASTIFRVESELLHIWDFSGQTTLLLMNDRTKSA 272

Query: 782  PVL---QNEGILLELQLFGLSHITTERHRVKDEMLVDHFNDEESFWTGSPKMNGSTG--D 946
                   +E ILLELQ++GL+     R   KD+ +V     E SF +GS KMNGSTG  D
Sbjct: 273  NDYLSRSDEQILLELQVYGLTDSMKGRGGKKDDQMVGQTKMEGSFVSGSLKMNGSTGNFD 332

Query: 947  SSNIAVDYQLSASLSKETCAMGLTGLLNVGNTCFMNSAIQCLVHSTKFVDYFLGDFQRDL 1126
            S       +L     +E   +GLTGL N+GNTCFMNSAIQCLVH+ K VDYFLGD+++++
Sbjct: 333  SYFTLPHTKLFGRGYREAGFLGLTGLHNIGNTCFMNSAIQCLVHTPKLVDYFLGDYRKEI 392

Query: 1127 NIENPLGMKGKLALAFGDLFRKLWTPGATPVAPIMFKSVLSDFAPQFSGYNQHDSQEFLA 1306
            N ENPLGM G+LALAFGDL RKLW PGATPVAP MFK  LS FAPQFSGYNQHDSQEFLA
Sbjct: 393  NYENPLGMNGELALAFGDLLRKLWAPGATPVAPRMFKLKLSSFAPQFSGYNQHDSQEFLA 452

Query: 1307 FLLDGLHEDLNRVRCKPYIEVKDADGVPDNELADEHWRNHLARNDSIIVDVCQGQFRSKL 1486
            FLLDGLHEDLNRV+CKPYIE  D DG PD E+ADE+WRNHLARNDSIIV++CQGQFRS L
Sbjct: 453  FLLDGLHEDLNRVKCKPYIEAGDPDGRPDEEVADEYWRNHLARNDSIIVNLCQGQFRSML 512

Query: 1487 VCPICKKVSIAFDPFMYLSLPLPSTLMRTMTITVLSTDGSSMPIPVTVTVPKFGRCEDLV 1666
            VCP+CKKVS+ FDPFMYLSLPLPST MRTMT+TVLSTDG+++P P TVTVPK GR +DL+
Sbjct: 513  VCPVCKKVSVTFDPFMYLSLPLPSTTMRTMTVTVLSTDGTTLPYPCTVTVPKCGRLKDLI 572

Query: 1667 QAVSSSCSLRDDEILLLAEVFNNCIIQFLDKPADSIDLLRDSDQLVAYRLPKDNN--DSS 1840
            QA+S +CSLR+DE LL+AE++NNCII++L++P+DS+ L+RD D+LVAYRL +D+   D+S
Sbjct: 573  QALSIACSLRNDERLLVAEIYNNCIIRYLEEPSDSLALIRDGDRLVAYRLSEDSKDCDTS 632

Query: 1841 HLVVFMHQHV---DITMSKYLTTWKKFGIPLLARMSDVSKGSEIRKQFLKLLNPFLLSAE 2011
             LVVFMH+ V   DI     L   K FGIPL+ RMS+ S G +I KQFLKLL+PF +  E
Sbjct: 633  SLVVFMHERVEKHDINGKAELCR-KMFGIPLVVRMSECSNGCDIHKQFLKLLDPFFMPTE 691

Query: 2012 D 2014
            D
Sbjct: 692  D 692


>ref|XP_003632213.1| PREDICTED: ubiquitin carboxyl-terminal hydrolase 8-like [Vitis
            vinifera]
          Length = 882

 Score =  605 bits (1559), Expect = e-170
 Identities = 324/603 (53%), Positives = 416/603 (68%), Gaps = 19/603 (3%)
 Frame = +2

Query: 266  DNSQLYLVQHSWWNEAHESVFS--RDVTGVSYLTTASLSKIEDFWEIDDFTSSEIVMEMK 439
            D+ ++Y V + WW EA +S  +      GV Y      S +     I++  +S++V  ++
Sbjct: 17   DDVRVYFVPYRWWKEAQDSNLADGNAKRGVLYSARPGSSYVGPMKIINNIFNSDLVFNLR 76

Query: 440  RDDDLQSSEE----GVSC-EYALLPDWMFLRALKWHNDVH---KDG-TIVTAKGTLVDLI 592
            R++D   S E    GVS  +YAL+P  ++L+ALKWH D     KDG T    +  + D+ 
Sbjct: 77   REEDSGHSNENGEVGVSGRDYALVPGDLWLQALKWHTDSRVATKDGRTFSVMEDDMADVY 136

Query: 593  SLKMRC-FLGKMNFLVAKISQKDNEVGMFKRACDIFCVEGDQFRIRDFSGQITQFTVNDR 769
             L +R   L + N L  KI++KDN V  F+RAC IF VE +   I DFSGQ T F +ND+
Sbjct: 137  PLLLRLSILRETNSLGVKITKKDNAVEFFRRACKIFSVESELLHIWDFSGQTTLFFINDK 196

Query: 770  NIPAPVLQNEG---ILLELQLFGLSHITTERHRVKDEMLVDHFNDEESFWTGSPKMNGST 940
            N      Q +    ILLELQ++GLS     R   KDEM   H N   S    S  MNGS 
Sbjct: 197  NKLPKDCQRQSDQEILLELQVYGLSDSLRCREGKKDEMAAQHSNLAGSSCGASLMMNGSL 256

Query: 941  G--DSSNIAVDYQLSASLSKETCAMGLTGLLNVGNTCFMNSAIQCLVHSTKFVDYFLGDF 1114
               +S+++  +  +    S E  ++GLTGL N+GNTCFMNS++QCL H+ K VDYFLGD+
Sbjct: 257  ACMNSNSVHTNSPVFYGSSGEAGSLGLTGLQNLGNTCFMNSSLQCLAHTPKLVDYFLGDY 316

Query: 1115 QRDLNIENPLGMKGKLALAFGDLFRKLWTPGATPVAPIMFKSVLSDFAPQFSGYNQHDSQ 1294
             R++N EN LGM G++ALAFGDL RKLW PGATPVAP MFKS L+ FAPQFSG+NQHDSQ
Sbjct: 317  SREINHENALGMDGEIALAFGDLIRKLWAPGATPVAPRMFKSKLARFAPQFSGFNQHDSQ 376

Query: 1295 EFLAFLLDGLHEDLNRVRCKPYIEVKDADGVPDNELADEHWRNHLARNDSIIVDVCQGQF 1474
            E LAFLLDGLHEDLNRV+CKPYIE KD +G PD E+ADE+WRNHLARNDSIIVDV QGQ+
Sbjct: 377  ELLAFLLDGLHEDLNRVKCKPYIEAKDGEGWPDEEVADEYWRNHLARNDSIIVDVSQGQY 436

Query: 1475 RSKLVCPICKKVSIAFDPFMYLSLPLPSTLMRTMTITVLSTDGSSMPIPVTVTVPKFGRC 1654
            RS LVCP+CKKVSI FDPFMYLSLPLPST MRTMT+TV+S DG + P P T+TVPK G+C
Sbjct: 437  RSTLVCPVCKKVSITFDPFMYLSLPLPSTTMRTMTLTVVSADGITQPSPCTITVPKNGKC 496

Query: 1655 EDLVQAVSSSCSLRDDEILLLAEVFNNCIIQFLDKPADSIDLLRDSDQLVAYRLPKDNND 1834
            EDL+QA+SSSC L+DDE LL+AE++NN II++L+ P DS+ L+RD D+LVAYRLPK  N+
Sbjct: 497  EDLIQALSSSCYLKDDETLLVAEIYNNRIIRYLEDPTDSLSLIRDGDRLVAYRLPKVTNE 556

Query: 1835 SSHLVVFMHQHVD--ITMSKYLTTWKKFGIPLLARMSDVSKGSEIRKQFLKLLNPFLLSA 2008
               LVVFMHQ +D      K  ++WK FGIPL+AR+ +   GS++   +LKL+NPF +++
Sbjct: 557  -DRLVVFMHQRIDEQYIHGKLSSSWKAFGIPLVARICNSVNGSDVYNLYLKLINPFQITS 615

Query: 2009 EDV 2017
            E +
Sbjct: 616  EGI 618


>ref|XP_002515876.1| Ubiquitin carboxyl-terminal hydrolase, putative [Ricinus communis]
            gi|223545031|gb|EEF46545.1| Ubiquitin carboxyl-terminal
            hydrolase, putative [Ricinus communis]
          Length = 889

 Score =  586 bits (1511), Expect = e-165
 Identities = 311/607 (51%), Positives = 423/607 (69%), Gaps = 20/607 (3%)
 Frame = +2

Query: 254  ETPTDNSQLYLVQHSWWNEAHESVFSRDVTG---VSYLTTASLSKIEDFWEIDDFTSSEI 424
            ++  ++ +LY V + WW +A +S  S +  G   V Y+ T+  S       I++  +S++
Sbjct: 19   DSDNNDQRLYFVPYRWWKDAQDSTISSESDGKRGVLYMGTSGSSYAGPMKLINNIFNSDL 78

Query: 425  VMEMKRDDD-LQSSEEGV----SCEYALLPDWMFLRALKWHNDVH----KDGTIVTAKGT 577
            V+ ++R+++ LQ+ E G       +YAL+   M+L+ALKWH+D         T   A+  
Sbjct: 79   VLNLRREEESLQNGENGEVGVSGRDYALVSGEMWLKALKWHSDAKVASKSSKTFSAAEDD 138

Query: 578  LVDLISLKMRC-FLGKMNFLVAKISQKDNEVGMFKRACDIFCVEGDQFRIRDFSGQITQF 754
            + D+  L++R   L ++N L  KIS+K+N   +F+RAC IF V+ +  RI DFSGQ T F
Sbjct: 139  MSDVYPLQLRLSVLREVNSLGVKISKKENATELFRRACKIFSVDSEMLRIWDFSGQTTLF 198

Query: 755  TVNDRNIPAPVLQ---NEGILLELQLFGLSHITTERHRVKDEMLVDHFNDEESFWTGSPK 925
             VND++      Q   ++ I+LELQ++GLS     R   KD+ +  + N   S  +G+  
Sbjct: 199  FVNDKSKSPKESQRQLDQEIVLELQVYGLSDNMKCRDGKKDDAVAQNCNGTHS--SGTSL 256

Query: 926  MNGSTGDSSNIAVDYQLSASLSK--ETCAMGLTGLLNVGNTCFMNSAIQCLVHSTKFVDY 1099
            MNGS  ++S        SA   K  E  ++GLTGL N+GNTCFMNSAIQCL H+ K VDY
Sbjct: 257  MNGSVSNTSTSFFRCDSSALFGKSGEAGSLGLTGLQNLGNTCFMNSAIQCLAHTPKLVDY 316

Query: 1100 FLGDFQRDLNIENPLGMKGKLALAFGDLFRKLWTPGATPVAPIMFKSVLSDFAPQFSGYN 1279
            FLGD+ R++N +NPLGM G++ALAFGDL RKLW PGATPVAP  FKS L+ FAPQFSG+N
Sbjct: 317  FLGDYGREINHDNPLGMDGEIALAFGDLLRKLWAPGATPVAPRTFKSKLARFAPQFSGFN 376

Query: 1280 QHDSQEFLAFLLDGLHEDLNRVRCKPYIEVKDADGVPDNELADEHWRNHLARNDSIIVDV 1459
            QHDSQE LAFLLDGLHEDLNRV+ KPY+E KD +G  D E+ADE+W+NH+ARNDSIIVD+
Sbjct: 377  QHDSQELLAFLLDGLHEDLNRVKSKPYVEAKDGEGRLDEEVADEYWQNHVARNDSIIVDI 436

Query: 1460 CQGQFRSKLVCPICKKVSIAFDPFMYLSLPLPSTLMRTMTITVLSTDGSSMPIPVTVTVP 1639
            CQGQ++S LVCPICKKVS+ FDPFMYLSLPLPST +RTMT+TV+STDGSS P   T++VP
Sbjct: 437  CQGQYKSTLVCPICKKVSVTFDPFMYLSLPLPSTNIRTMTLTVVSTDGSSQPSSFTISVP 496

Query: 1640 KFGRCEDLVQAVSSSCSLRDDEILLLAEVFNNCIIQFLDKPADSIDLLRDSDQLVAYRLP 1819
            K+G+CEDL QA+S +CSL  DE+LL+AEV+NN II++L++P+DS+ L+RD D+LVAYRL 
Sbjct: 497  KYGKCEDLTQALSKACSLGIDEMLLVAEVYNNRIIRYLEEPSDSLSLIRDGDRLVAYRLK 556

Query: 1820 KDNNDSSHLVVFMHQHVD--ITMSKYLTTWKKFGIPLLARMSDVSKGSEIRKQFLKLLNP 1993
            K+  D+  LVVFMHQH++      K  ++WK FGIPL+  +  +  GS+I   +LKLL+P
Sbjct: 557  KE-LDNIPLVVFMHQHMEEQYVHGKQTSSWKAFGIPLVGPVHSLVNGSDIHNLYLKLLSP 615

Query: 1994 FLLSAED 2014
            F + A+D
Sbjct: 616  FHIPADD 622


>emb|CBI24406.3| unnamed protein product [Vitis vinifera]
          Length = 921

 Score =  559 bits (1441), Expect = e-157
 Identities = 301/551 (54%), Positives = 377/551 (68%), Gaps = 15/551 (2%)
 Frame = +2

Query: 266  DNSQLYLVQHSWWNEAHESVFS--RDVTGVSYLTTASLSKIEDFWEIDDFTSSEIVMEMK 439
            D+ ++Y V + WW EA +S  +      GV Y      S +     I++  +S++V  ++
Sbjct: 17   DDVRVYFVPYRWWKEAQDSNLADGNAKRGVLYSARPGSSYVGPMKIINNIFNSDLVFNLR 76

Query: 440  RDDDLQSSEE----GVSC-EYALLPDWMFLRALKWHNDVH---KDG-TIVTAKGTLVDLI 592
            R++D   S E    GVS  +YAL+P  ++L+ALKWH D     KDG T    +  + D+ 
Sbjct: 77   REEDSGHSNENGEVGVSGRDYALVPGDLWLQALKWHTDSRVATKDGRTFSVMEDDMADVY 136

Query: 593  SLKMRC-FLGKMNFLVAKISQKDNEVGMFKRACDIFCVEGDQFRIRDFSGQITQFTVNDR 769
             L +R   L + N L  KI++KDN V  F+RAC IF VE +   I DFSGQ T F +ND+
Sbjct: 137  PLLLRLSILRETNSLGVKITKKDNAVEFFRRACKIFSVESELLHIWDFSGQTTLFFINDK 196

Query: 770  NIPAPVLQNEG---ILLELQLFGLSHITTERHRVKDEMLVDHFNDEESFWTGSPKMNGST 940
            N      Q +    ILLELQ++GLS     R   KDEM   H N       GS    GS+
Sbjct: 197  NKLPKDCQRQSDQEILLELQVYGLSDSLRCREGKKDEMAAQHSN-----LAGSSFFYGSS 251

Query: 941  GDSSNIAVDYQLSASLSKETCAMGLTGLLNVGNTCFMNSAIQCLVHSTKFVDYFLGDFQR 1120
            G++ ++                 GLTGL N+GNTCFMNS++QCL H+ K VDYFLGD+ R
Sbjct: 252  GEAGSL-----------------GLTGLQNLGNTCFMNSSLQCLAHTPKLVDYFLGDYSR 294

Query: 1121 DLNIENPLGMKGKLALAFGDLFRKLWTPGATPVAPIMFKSVLSDFAPQFSGYNQHDSQEF 1300
            ++N EN LGM G++ALAFGDL RKLW PGATPVAP MFKS L+ FAPQFSG+NQHDSQE 
Sbjct: 295  EINHENALGMDGEIALAFGDLIRKLWAPGATPVAPRMFKSKLARFAPQFSGFNQHDSQEL 354

Query: 1301 LAFLLDGLHEDLNRVRCKPYIEVKDADGVPDNELADEHWRNHLARNDSIIVDVCQGQFRS 1480
            LAFLLDGLHEDLNRV+CKPYIE KD +G PD E+ADE+WRNHLARNDSIIVDV QGQ+RS
Sbjct: 355  LAFLLDGLHEDLNRVKCKPYIEAKDGEGWPDEEVADEYWRNHLARNDSIIVDVSQGQYRS 414

Query: 1481 KLVCPICKKVSIAFDPFMYLSLPLPSTLMRTMTITVLSTDGSSMPIPVTVTVPKFGRCED 1660
             LVCP+CKKVSI FDPFMYLSLPLPST MRTMT+TV+S DG + P P T+TVPK G+CED
Sbjct: 415  TLVCPVCKKVSITFDPFMYLSLPLPSTTMRTMTLTVVSADGITQPSPCTITVPKNGKCED 474

Query: 1661 LVQAVSSSCSLRDDEILLLAEVFNNCIIQFLDKPADSIDLLRDSDQLVAYRLPKDNNDSS 1840
            L+QA+SSSC L+DDE LL+AE++NN II++L+ P DS+ L+RD D+LVAYRLPK  N+  
Sbjct: 475  LIQALSSSCYLKDDETLLVAEIYNNRIIRYLEDPTDSLSLIRDGDRLVAYRLPKVTNE-D 533

Query: 1841 HLVVFMHQHVD 1873
             LVVFMHQ +D
Sbjct: 534  RLVVFMHQRID 544


>ref|XP_004140175.1| PREDICTED: ubiquitin carboxyl-terminal hydrolase 8-like [Cucumis
            sativus]
          Length = 877

 Score =  555 bits (1430), Expect = e-155
 Identities = 304/608 (50%), Positives = 417/608 (68%), Gaps = 17/608 (2%)
 Frame = +2

Query: 242  ALPFETPTDNSQLYLVQHSWWNEAHESVF--SRDVTGVSYLTTASLSKIEDFWEIDDFTS 415
            +L   + TD  ++Y V + WW +A +S+   +    G S+L +   S       I+   S
Sbjct: 12   SLNLHSDTDQ-RVYFVPYRWWKDAQDSLTGDADGKRGHSFLASPGSSYAGPMKIINSIFS 70

Query: 416  SEIVMEMKRDDDLQSSEE----GVSC-EYALLPDWMFLRALKWHND----VHKDGTIVTA 568
            S++V  ++R++D   + E    G+S  +YAL+   M++ ALKWH+D        G+   A
Sbjct: 71   SDLVFNLRREEDSSQNSENGEVGISGRDYALVSGEMWVEALKWHSDSKSSTRNGGSFSVA 130

Query: 569  KGTLVDLISLKMRCFLGK-MNFLVAKISQKDNEVGMFKRACDIFCVEGDQFRIRDFSGQ- 742
            +  + D+  L++R  + +  N L  +IS+KDN V +F+RAC +F VE +  RI DFSG  
Sbjct: 131  EDNIADVYPLQLRLSIQREANLLGVRISKKDNVVELFRRACKLFNVESELLRIWDFSGHT 190

Query: 743  ITQFTVNDRNIPAPVLQNEGILLELQLFGLSHITTERHRVKDEMLVDHFNDEESFWTGSP 922
            I+ F+ +++   +    ++ +LLELQ++GLS     +   KDE+      +  +    S 
Sbjct: 191  ISLFSNSNKQRQS----DQEVLLELQVYGLSDFIKCKEGKKDELANSILGNSSAT---SL 243

Query: 923  KMNGS--TGDSSNIAVDYQLSASLSKETCAMGLTGLLNVGNTCFMNSAIQCLVHSTKFVD 1096
             MNG+  TG +S   V+  + +  S+E  ++GLTGL N+GNTCFMNSA+QCLVH+ K VD
Sbjct: 244  MMNGASGTGSASCFRVNSSVFSGSSREAGSLGLTGLQNLGNTCFMNSALQCLVHTPKLVD 303

Query: 1097 YFLGDFQRDLNIENPLGMKGKLALAFGDLFRKLWTPGATPVAPIMFKSVLSDFAPQFSGY 1276
            YFLGD+ R++N +NPLGM G++ALAFGD+ RKLW PGA+PVAP  FKS L+ FAPQFSG 
Sbjct: 304  YFLGDYGREINHDNPLGMNGEIALAFGDMLRKLWAPGASPVAPRTFKSKLARFAPQFSGC 363

Query: 1277 NQHDSQEFLAFLLDGLHEDLNRVRCKPYIEVKDADGVPDNELADEHWRNHLARNDSIIVD 1456
            NQHDSQE LAFLLDGLHEDLNRV+ KPY+E KD DG  DNE+ADE+W+NHLARNDSIIVD
Sbjct: 364  NQHDSQELLAFLLDGLHEDLNRVKKKPYVEAKDGDGRSDNEVADEYWQNHLARNDSIIVD 423

Query: 1457 VCQGQFRSKLVCPICKKVSIAFDPFMYLSLPLPSTLMRTMTITVLSTDGSSMPIPVTVTV 1636
            VCQGQ++S LVCPICKKVS  FDPFMYLSLPLPST MRTMT+TV+STDGS+ P P T+TV
Sbjct: 424  VCQGQYKSTLVCPICKKVSTTFDPFMYLSLPLPSTTMRTMTLTVVSTDGST-PAPYTITV 482

Query: 1637 PKFGRCEDLVQAVSSSCSLRDDEILLLAEVFNNCIIQFLDKPADSIDLLRDSDQLVAYRL 1816
            PK G+ EDL++A+S +CSL+ DE LL+AEV+NN II++L++PADS+ L+RD D+LVAYRL
Sbjct: 483  PKSGKWEDLIKALSIACSLKADETLLVAEVYNNRIIRYLEEPADSLSLIRDGDRLVAYRL 542

Query: 1817 PKDNNDSSHLVVFMHQHVD--ITMSKYLTTWKKFGIPLLARMSDVSKGSEIRKQFLKLLN 1990
             KD  +   L VFMHQ ++      K  T+ K FG PL+A++     GS+I+  + KLL+
Sbjct: 543  AKD-EEQVPLAVFMHQRIEEQYINGKRTTSLKAFGFPLVAKLPGGFNGSDIKDIYKKLLS 601

Query: 1991 PFLLSAED 2014
            P+ +SA D
Sbjct: 602  PYQVSAPD 609