BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018978
         (952 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago tr...   175   1e-41
ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thalian...   171   3e-40
gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidops...   171   3e-40
ref|XP_003523464.1| PREDICTED: biotin--protein ligase-like [Glyc...   170   5e-40
ref|XP_003526610.1| PREDICTED: biotin--protein ligase-like [Glyc...   169   7e-40

>ref|XP_003601098.1| Holocarboxylase synthetase hcs2 [Medicago truncatula]
           gi|355490146|gb|AES71349.1| Holocarboxylase synthetase
           hcs2 [Medicago truncatula]
          Length = 361

 Score =  175 bits (444), Expect = 1e-41
 Identities = 87/129 (67%), Positives = 104/129 (80%)
 Frame = -1

Query: 640 FPCSPGIGLNVDNVEPTTSLNAVLRNLTSGSYRIKREDILSAXXXXXXXXXXXFMDQGFQ 461
           F  S GIGLNV+N +PTTSLN VLR L+ G+Y+ +RED+L+A           F++QGFQ
Sbjct: 228 FNISAGIGLNVNNEKPTTSLNTVLRELSVGAYQFQREDVLAAFFNKFEKFFDLFLNQGFQ 287

Query: 460 PLEELYYKTWLHSEQRVIVEEKDDDKDQVIQSVVTVQGLTSSGYLLAVGDDNQMCELHPD 281
            LEELYYKTWLHS QRVIV+EK+++K  V++  VT+QGLTSSGYLLAVGDDNQMCELHPD
Sbjct: 288 TLEELYYKTWLHSGQRVIVQEKNEEK--VVEHAVTIQGLTSSGYLLAVGDDNQMCELHPD 345

Query: 280 GNSFDFFKG 254
           GNSFDFFKG
Sbjct: 346 GNSFDFFKG 354



 Score = 83.6 bits (205), Expect = 6e-14
 Identities = 35/46 (76%), Positives = 41/46 (89%)
 Frame = -1

Query: 946 VCDKNGFPYLNVRIKWPNDLYLDDLKVGGILCTSTYRSKKFNVSVG 809
           +CDKNG P ++V+IKWPNDLYL+  KVGGILCTSTYRSKKFN+S G
Sbjct: 188 ICDKNGLPCVDVKIKWPNDLYLNGFKVGGILCTSTYRSKKFNISAG 233


>ref|NP_565605.1| holocarboxylase synthase 1 [Arabidopsis thaliana]
           gi|30682866|ref|NP_850067.1| holocarboxylase synthase 1
           [Arabidopsis thaliana] gi|1688072|gb|AAC49706.1| biotin
           holocarboxylase synthetase [Arabidopsis thaliana]
           gi|20197509|gb|AAD31371.2| biotin holocarboxylase
           synthetase [Arabidopsis thaliana]
           gi|89000993|gb|ABD59086.1| At2g25710 [Arabidopsis
           thaliana] gi|330252646|gb|AEC07740.1| holocarboxylase
           synthase 1 [Arabidopsis thaliana]
           gi|330252647|gb|AEC07741.1| holocarboxylase synthase 1
           [Arabidopsis thaliana]
          Length = 367

 Score =  171 bits (432), Expect = 3e-40
 Identities = 88/129 (68%), Positives = 101/129 (78%)
 Frame = -1

Query: 640 FPCSPGIGLNVDNVEPTTSLNAVLRNLTSGSYRIKREDILSAXXXXXXXXXXXFMDQGFQ 461
           F  S G+GLNVDN +PTT LNAVL+ +   S  +KRE+IL A           FMDQGF+
Sbjct: 235 FNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILGAFFHKFEKFFDLFMDQGFK 294

Query: 460 PLEELYYKTWLHSEQRVIVEEKDDDKDQVIQSVVTVQGLTSSGYLLAVGDDNQMCELHPD 281
            LEELYY+TWLHSEQRVIVE+K +  DQV+Q+VVT+QGLTSSGYLLAVGDDNQM ELHPD
Sbjct: 295 SLEELYYRTWLHSEQRVIVEDKVE--DQVVQNVVTIQGLTSSGYLLAVGDDNQMYELHPD 352

Query: 280 GNSFDFFKG 254
           GNSFDFFKG
Sbjct: 353 GNSFDFFKG 361



 Score = 87.8 bits (216), Expect = 3e-15
 Identities = 38/46 (82%), Positives = 43/46 (93%)
 Frame = -1

Query: 946 VCDKNGFPYLNVRIKWPNDLYLDDLKVGGILCTSTYRSKKFNVSVG 809
           VCDK G PY++V+IKWPNDLY++ LKVGGILCTSTYRSKKFNVSVG
Sbjct: 195 VCDKKGLPYIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVG 240


>gb|AAL93108.1|AF414937_1 holocarboxylase synthetase 1 [Arabidopsis thaliana]
          Length = 367

 Score =  171 bits (432), Expect = 3e-40
 Identities = 88/129 (68%), Positives = 101/129 (78%)
 Frame = -1

Query: 640 FPCSPGIGLNVDNVEPTTSLNAVLRNLTSGSYRIKREDILSAXXXXXXXXXXXFMDQGFQ 461
           F  S G+GLNVDN +PTT LNAVL+ +   S  +KRE+IL A           FMDQGF+
Sbjct: 235 FNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEILGAFFHKFEKFFDLFMDQGFK 294

Query: 460 PLEELYYKTWLHSEQRVIVEEKDDDKDQVIQSVVTVQGLTSSGYLLAVGDDNQMCELHPD 281
            LEELYY+TWLHSEQRVIVE+K +  DQV+Q+VVT+QGLTSSGYLLAVGDDNQM ELHPD
Sbjct: 295 SLEELYYRTWLHSEQRVIVEDKVE--DQVVQNVVTIQGLTSSGYLLAVGDDNQMYELHPD 352

Query: 280 GNSFDFFKG 254
           GNSFDFFKG
Sbjct: 353 GNSFDFFKG 361



 Score = 87.8 bits (216), Expect = 3e-15
 Identities = 38/46 (82%), Positives = 43/46 (93%)
 Frame = -1

Query: 946 VCDKNGFPYLNVRIKWPNDLYLDDLKVGGILCTSTYRSKKFNVSVG 809
           VCDK G PY++V+IKWPNDLY++ LKVGGILCTSTYRSKKFNVSVG
Sbjct: 195 VCDKKGLPYIDVKIKWPNDLYVNGLKVGGILCTSTYRSKKFNVSVG 240


>ref|XP_003523464.1| PREDICTED: biotin--protein ligase-like [Glycine max]
          Length = 361

 Score =  170 bits (430), Expect = 5e-40
 Identities = 84/129 (65%), Positives = 103/129 (79%)
 Frame = -1

Query: 640 FPCSPGIGLNVDNVEPTTSLNAVLRNLTSGSYRIKREDILSAXXXXXXXXXXXFMDQGFQ 461
           F  S GIGLNV+N +PTTSLN +L+   SG+Y+ +RE++L+A           F++QGFQ
Sbjct: 228 FNVSAGIGLNVNNKKPTTSLNTILKGFYSGAYQFQREEVLAAFFNKFEIFYDLFLNQGFQ 287

Query: 460 PLEELYYKTWLHSEQRVIVEEKDDDKDQVIQSVVTVQGLTSSGYLLAVGDDNQMCELHPD 281
            LEELY KTWLHS QRV+V+EK++DK  VI+ VVT+QGLTSSGYLLAVGDD+QMCELHPD
Sbjct: 288 TLEELYIKTWLHSGQRVVVQEKNEDK--VIEHVVTIQGLTSSGYLLAVGDDHQMCELHPD 345

Query: 280 GNSFDFFKG 254
           GNSFDFFKG
Sbjct: 346 GNSFDFFKG 354



 Score = 82.4 bits (202), Expect = 1e-13
 Identities = 35/46 (76%), Positives = 41/46 (89%)
 Frame = -1

Query: 946 VCDKNGFPYLNVRIKWPNDLYLDDLKVGGILCTSTYRSKKFNVSVG 809
           +CDKNG P ++V+IKWPNDLYL+  KVGGILCTSTY+SKKFNVS G
Sbjct: 188 ICDKNGLPSIDVKIKWPNDLYLNGSKVGGILCTSTYKSKKFNVSAG 233


>ref|XP_003526610.1| PREDICTED: biotin--protein ligase-like [Glycine max]
          Length = 361

 Score =  169 bits (429), Expect = 7e-40
 Identities = 84/129 (65%), Positives = 101/129 (78%)
 Frame = -1

Query: 640 FPCSPGIGLNVDNVEPTTSLNAVLRNLTSGSYRIKREDILSAXXXXXXXXXXXFMDQGFQ 461
           F  S GIGLNV+N  PTTSLN +L+   +G+Y+ +RED+L+A           F++QGFQ
Sbjct: 228 FNVSAGIGLNVNNKNPTTSLNTILKGFYTGAYQFQREDVLAAFFNKFEKFYDLFVNQGFQ 287

Query: 460 PLEELYYKTWLHSEQRVIVEEKDDDKDQVIQSVVTVQGLTSSGYLLAVGDDNQMCELHPD 281
            LEELY KTWLHS QRV+V+EK++DK  VI+ VVT+QGLTSSGYLLAVGDDN MCELHPD
Sbjct: 288 TLEELYIKTWLHSGQRVVVQEKNEDK--VIEHVVTIQGLTSSGYLLAVGDDNHMCELHPD 345

Query: 280 GNSFDFFKG 254
           GNSFDFFKG
Sbjct: 346 GNSFDFFKG 354



 Score = 84.0 bits (206), Expect = 5e-14
 Identities = 36/46 (78%), Positives = 41/46 (89%)
 Frame = -1

Query: 946 VCDKNGFPYLNVRIKWPNDLYLDDLKVGGILCTSTYRSKKFNVSVG 809
           +CDKNG P ++VRIKWPNDLYL+  KVGGILCTSTY+SKKFNVS G
Sbjct: 188 ICDKNGLPSIDVRIKWPNDLYLNGCKVGGILCTSTYKSKKFNVSAG 233