BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018827
         (1477 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002277239.1| PREDICTED: probable polygalacturonase [Vitis...   776   0.0  
ref|XP_002516244.1| polygalacturonase, putative [Ricinus communi...   773   0.0  
ref|XP_002326133.1| predicted protein [Populus trichocarpa] gi|2...   773   0.0  
ref|XP_003551102.1| PREDICTED: probable polygalacturonase-like i...   772   0.0  
ref|XP_003542367.1| PREDICTED: probable polygalacturonase-like [...   770   0.0  

>ref|XP_002277239.1| PREDICTED: probable polygalacturonase [Vitis vinifera]
            gi|297738532|emb|CBI27777.3| unnamed protein product
            [Vitis vinifera]
          Length = 493

 Score =  776 bits (2004), Expect = 0.0
 Identities = 365/460 (79%), Positives = 414/460 (90%)
 Frame = +3

Query: 93   FLHRRLDFRRYFPASVTSHTTLFTLLCILAVSSLFIWYRSFVVQLSILRLTGPVTRPIPE 272
            F H R D +R+ PA ++SH TLFT+L I A +S+F+W R+ V  L   R      RP+P 
Sbjct: 12   FNHHRPDLKRWIPAFLSSHRTLFTILWIAAFASVFVWQRNIVEGLLTFRRAP--ARPLPR 69

Query: 273  LRPVVFNLTEFGGIGDGVTLNTKAFERAVIAISKFAKKGGAQLNVPAGNWLTAPFNLTSH 452
            LRP V+NLT+FGG+GD VT+NT+AFERA+ AISK  KKGG QLNVPAGNWLTAPFNLTSH
Sbjct: 70   LRPAVYNLTDFGGVGDSVTVNTEAFERAISAISKLGKKGGGQLNVPAGNWLTAPFNLTSH 129

Query: 453  MTLFLAQDAVILGVDDEKYWSLLPPLPSYGHGREHRGARYGSLIHGQHLKDVVITGHNGT 632
            MTLFL QDAVILG+ DEKYW L+PPLPSYG+GREHRGARYGSLIHGQ+LKDVVITGHNGT
Sbjct: 130  MTLFLDQDAVILGIQDEKYWPLMPPLPSYGYGREHRGARYGSLIHGQNLKDVVITGHNGT 189

Query: 633  INGQGRSWWKKYRQKSLNHTRGPLVQIMWSSDILISNITLCDSPFWTLHPYDCKNVTIRH 812
            INGQG++WWKKYRQK LNHTRGPLVQIMWSSDI+ISNITL DSPFWTLHPYDCKNVTI++
Sbjct: 190  INGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVISNITLRDSPFWTLHPYDCKNVTIKN 249

Query: 813  MTILAPISGAPNTDGIDPDSCENMIIEDCYISVGDDAIAIKSGWDQYGISYGRPSKNILI 992
            +TILAPI  APNTDGIDPDSCE+M+IEDCYISVGDD IAIKSGWDQYG++YGRPS NILI
Sbjct: 250  VTILAPIFEAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGVAYGRPSVNILI 309

Query: 993  RNLVVRSMVSAGISIGSEMSGGVSNVTVENVLVWNSRRAVRIKTAIGRGGYVRHITYRNL 1172
            RNLV+RSMVSAG+SIGSEMSGGVSNVTVEN+LVWNSRRAVRIKT+ GRGGYV+HI YRNL
Sbjct: 310  RNLVIRSMVSAGVSIGSEMSGGVSNVTVENLLVWNSRRAVRIKTSPGRGGYVQHINYRNL 369

Query: 1173 TFENVRVGIVIKTDYNEHPDKGFNPKAIPVLQDISYSSIHGEGVRVPVRIEGSQEIPVRN 1352
            TF+NVRVGIVIKTDYNEHPD GF+PKA+P+L+DIS++ +HG+GVRVPVRI GS+EIPV+ 
Sbjct: 370  TFDNVRVGIVIKTDYNEHPDDGFDPKALPILEDISFTGVHGQGVRVPVRIHGSEEIPVKK 429

Query: 1353 VTFHDMSVGLTYKKKHIFQCAYVQGRVIGTIFPAPCENLD 1472
            VTF DMSVG+TYKKKHIFQCAYV+GRVIGT+FPAPCENLD
Sbjct: 430  VTFRDMSVGITYKKKHIFQCAYVEGRVIGTVFPAPCENLD 469


>ref|XP_002516244.1| polygalacturonase, putative [Ricinus communis]
            gi|223544730|gb|EEF46246.1| polygalacturonase, putative
            [Ricinus communis]
          Length = 494

 Score =  773 bits (1997), Expect = 0.0
 Identities = 365/461 (79%), Positives = 416/461 (90%)
 Frame = +3

Query: 90   RFLHRRLDFRRYFPASVTSHTTLFTLLCILAVSSLFIWYRSFVVQLSILRLTGPVTRPIP 269
            R  ++RL+ +R+ P   +SH TLFT+L I A +S+F+W R+ V     +       RP+P
Sbjct: 10   RLHYQRLELKRWVPTFFSSHKTLFTVLWIAAFASVFVWQRNVVGDGFAVFFKASPMRPMP 69

Query: 270  ELRPVVFNLTEFGGIGDGVTLNTKAFERAVIAISKFAKKGGAQLNVPAGNWLTAPFNLTS 449
            +LRPV FNLT+FGG+GDGVTLNT+AFERAV+AISK  KKGG QLNVP G W+TAPFNLTS
Sbjct: 70   KLRPVAFNLTDFGGVGDGVTLNTEAFERAVLAISKLGKKGGGQLNVPPGRWVTAPFNLTS 129

Query: 450  HMTLFLAQDAVILGVDDEKYWSLLPPLPSYGHGREHRGARYGSLIHGQHLKDVVITGHNG 629
            HMTLFLA+D++ILG++DEKYW L+PPLPSYG+GREH G RYGSLIHGQ+LKDVVITGHNG
Sbjct: 130  HMTLFLAEDSIILGIEDEKYWPLMPPLPSYGYGREHPGPRYGSLIHGQNLKDVVITGHNG 189

Query: 630  TINGQGRSWWKKYRQKSLNHTRGPLVQIMWSSDILISNITLCDSPFWTLHPYDCKNVTIR 809
            TINGQG++WWKKYRQK LNHTRGPLVQIMWSSDILI+NITL DSPFWTLHPYDCKNVTIR
Sbjct: 190  TINGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDILITNITLRDSPFWTLHPYDCKNVTIR 249

Query: 810  HMTILAPISGAPNTDGIDPDSCENMIIEDCYISVGDDAIAIKSGWDQYGISYGRPSKNIL 989
            ++TILAP+  APNTDGIDPDSCE+M+IEDCYISVGDD IAIKSGWDQYGI+Y RPS NIL
Sbjct: 250  NVTILAPVLEAPNTDGIDPDSCEDMVIEDCYISVGDDGIAIKSGWDQYGIAYRRPSTNIL 309

Query: 990  IRNLVVRSMVSAGISIGSEMSGGVSNVTVENVLVWNSRRAVRIKTAIGRGGYVRHITYRN 1169
            IRNLVVRSMVSAGISIGSEMSGGVSN+TVEN+LVW+SRRAVRIKTA GRGGYVRHITYRN
Sbjct: 310  IRNLVVRSMVSAGISIGSEMSGGVSNITVENLLVWSSRRAVRIKTAPGRGGYVRHITYRN 369

Query: 1170 LTFENVRVGIVIKTDYNEHPDKGFNPKAIPVLQDISYSSIHGEGVRVPVRIEGSQEIPVR 1349
            LTF+NVRVGIVIKTDYNEHPD+G++PKA+PVL+DIS++ +HG+GVRVPVRI GS+EIPVR
Sbjct: 370  LTFDNVRVGIVIKTDYNEHPDEGYDPKAVPVLRDISFTGVHGQGVRVPVRIHGSEEIPVR 429

Query: 1350 NVTFHDMSVGLTYKKKHIFQCAYVQGRVIGTIFPAPCENLD 1472
            NVTF DMSVGLTYKKKHIFQCA+VQGRVIGTIFPAPCENLD
Sbjct: 430  NVTFQDMSVGLTYKKKHIFQCAFVQGRVIGTIFPAPCENLD 470


>ref|XP_002326133.1| predicted protein [Populus trichocarpa] gi|222833326|gb|EEE71803.1|
            predicted protein [Populus trichocarpa]
          Length = 495

 Score =  773 bits (1996), Expect = 0.0
 Identities = 373/472 (79%), Positives = 423/472 (89%), Gaps = 2/472 (0%)
 Frame = +3

Query: 63   MVESV--PPSPRFLHRRLDFRRYFPASVTSHTTLFTLLCILAVSSLFIWYRSFVVQLSIL 236
            MVE++  PPS RF ++RL+ +R  PA ++SH TLF LL I A +S+F+W R+ V     +
Sbjct: 1    MVETIAPPPSSRFNYKRLELKRCLPAFLSSHKTLFALLWIAAFASVFLWKRNPVGAGLAV 60

Query: 237  RLTGPVTRPIPELRPVVFNLTEFGGIGDGVTLNTKAFERAVIAISKFAKKGGAQLNVPAG 416
               GP+ RP+P+LRPV FNLT+FG +GDG T+NT+AFERAV AISK +K+GG QLNVP G
Sbjct: 61   FWRGPL-RPMPQLRPVAFNLTDFGAVGDGATVNTEAFERAVSAISKLSKRGGGQLNVPPG 119

Query: 417  NWLTAPFNLTSHMTLFLAQDAVILGVDDEKYWSLLPPLPSYGHGREHRGARYGSLIHGQH 596
             WLTAPFNLTSHMTLFLA+ AVILG+ DEKYW L+P LPSYG+GREH G RYGSLIHGQ+
Sbjct: 120  RWLTAPFNLTSHMTLFLAEGAVILGIQDEKYWPLMPALPSYGYGREHPGPRYGSLIHGQN 179

Query: 597  LKDVVITGHNGTINGQGRSWWKKYRQKSLNHTRGPLVQIMWSSDILISNITLCDSPFWTL 776
            L+DVVITGHNGTI+GQG++WWKKYRQK LNHTRGPLVQIMWSSDI+ +NITL DSPFWTL
Sbjct: 180  LRDVVITGHNGTIDGQGQTWWKKYRQKLLNHTRGPLVQIMWSSDIVFTNITLRDSPFWTL 239

Query: 777  HPYDCKNVTIRHMTILAPISGAPNTDGIDPDSCENMIIEDCYISVGDDAIAIKSGWDQYG 956
            HPYDCKNVTIR++TILAPI  APNTDGIDPDSCE+M+IEDCYISVGDDAIAIKSGWDQYG
Sbjct: 240  HPYDCKNVTIRNVTILAPIFEAPNTDGIDPDSCEDMVIEDCYISVGDDAIAIKSGWDQYG 299

Query: 957  ISYGRPSKNILIRNLVVRSMVSAGISIGSEMSGGVSNVTVENVLVWNSRRAVRIKTAIGR 1136
            I+YGRPS NILIRNLVVRSMVSAGISIGSEMSGGVSNVTVENVLVW+SRRAVRIKTA GR
Sbjct: 300  IAYGRPSTNILIRNLVVRSMVSAGISIGSEMSGGVSNVTVENVLVWSSRRAVRIKTAPGR 359

Query: 1137 GGYVRHITYRNLTFENVRVGIVIKTDYNEHPDKGFNPKAIPVLQDISYSSIHGEGVRVPV 1316
            GGYVRHITYRNLTF+NVRVGIVIKTDYNEHPD+G++PKA+P+L DIS++ IHG+GVRVPV
Sbjct: 360  GGYVRHITYRNLTFDNVRVGIVIKTDYNEHPDEGYDPKAVPILGDISFTGIHGQGVRVPV 419

Query: 1317 RIEGSQEIPVRNVTFHDMSVGLTYKKKHIFQCAYVQGRVIGTIFPAPCENLD 1472
            RI GSQEIPVRNVTF DMSVGLTYKKKHIFQCA+VQGRVIGT+FPAPCENLD
Sbjct: 420  RIHGSQEIPVRNVTFWDMSVGLTYKKKHIFQCAFVQGRVIGTVFPAPCENLD 471


>ref|XP_003551102.1| PREDICTED: probable polygalacturonase-like isoform 1 [Glycine max]
          Length = 492

 Score =  772 bits (1994), Expect = 0.0
 Identities = 367/460 (79%), Positives = 414/460 (90%)
 Frame = +3

Query: 93   FLHRRLDFRRYFPASVTSHTTLFTLLCILAVSSLFIWYRSFVVQLSILRLTGPVTRPIPE 272
            F H+RLD RR+ PA  TSH TL TLL I A +S+F+W R+ V     L   G   RP+P 
Sbjct: 11   FHHQRLDLRRWVPAFFTSHKTLLTLLWIAAFASVFLWQRNIVG--GFLVYGGIPGRPMPM 68

Query: 273  LRPVVFNLTEFGGIGDGVTLNTKAFERAVIAISKFAKKGGAQLNVPAGNWLTAPFNLTSH 452
            LRP+ FNLT+FGG+GDGVTLNT+AFERAV A+SKF KKGGAQLNVP G WLTAPFNLTSH
Sbjct: 69   LRPMAFNLTDFGGVGDGVTLNTEAFERAVSAVSKFGKKGGAQLNVPPGRWLTAPFNLTSH 128

Query: 453  MTLFLAQDAVILGVDDEKYWSLLPPLPSYGHGREHRGARYGSLIHGQHLKDVVITGHNGT 632
            MTLFLA+DAVILG+DDEKYW L+PPLPSYG+GREH G RYGSLIHGQHLKDVVITGHNGT
Sbjct: 129  MTLFLAEDAVILGIDDEKYWPLMPPLPSYGYGREHPGPRYGSLIHGQHLKDVVITGHNGT 188

Query: 633  INGQGRSWWKKYRQKSLNHTRGPLVQIMWSSDILISNITLCDSPFWTLHPYDCKNVTIRH 812
            INGQG+SWWKKYRQK LNHTRGPLVQIM+SSDI+I+NITL DSPFWT+HPYDCKN+TI+ 
Sbjct: 189  INGQGQSWWKKYRQKRLNHTRGPLVQIMFSSDIVITNITLRDSPFWTIHPYDCKNITIKG 248

Query: 813  MTILAPISGAPNTDGIDPDSCENMIIEDCYISVGDDAIAIKSGWDQYGISYGRPSKNILI 992
            +TILAP+ GAPNTDGIDPDSCE+M+IEDCYISVGDDAIA+KSGWDQYGI YGRPS NI+I
Sbjct: 249  VTILAPVFGAPNTDGIDPDSCEDMLIEDCYISVGDDAIAVKSGWDQYGIDYGRPSMNIMI 308

Query: 993  RNLVVRSMVSAGISIGSEMSGGVSNVTVENVLVWNSRRAVRIKTAIGRGGYVRHITYRNL 1172
            RNLVVRSMVSAGISIGSEMSGGVSNVTVEN+L+W+SRR VRIKTA GRG YVR ITYRN+
Sbjct: 309  RNLVVRSMVSAGISIGSEMSGGVSNVTVENLLIWDSRRGVRIKTAPGRGAYVRQITYRNI 368

Query: 1173 TFENVRVGIVIKTDYNEHPDKGFNPKAIPVLQDISYSSIHGEGVRVPVRIEGSQEIPVRN 1352
            TFENVRVGIV+KTDYNEHPD G++P A+P+L+DIS++++HG+GVRVPVRI GS+EIPVRN
Sbjct: 369  TFENVRVGIVMKTDYNEHPDDGYDPLALPILRDISFTTVHGQGVRVPVRIHGSEEIPVRN 428

Query: 1353 VTFHDMSVGLTYKKKHIFQCAYVQGRVIGTIFPAPCENLD 1472
            VTF DMSVGLTYKKKHIFQCA+VQGRVIGTI+PAPCENLD
Sbjct: 429  VTFKDMSVGLTYKKKHIFQCAFVQGRVIGTIYPAPCENLD 468


>ref|XP_003542367.1| PREDICTED: probable polygalacturonase-like [Glycine max]
          Length = 491

 Score =  770 bits (1989), Expect = 0.0
 Identities = 365/461 (79%), Positives = 414/461 (89%)
 Frame = +3

Query: 90   RFLHRRLDFRRYFPASVTSHTTLFTLLCILAVSSLFIWYRSFVVQLSILRLTGPVTRPIP 269
            RF H+RLD RR+ PA  TSH TL T L I A +S+F+W R+  +    L   G   RP+P
Sbjct: 9    RFHHQRLDLRRWVPAFFTSHKTLLTFLWIAAFASVFLWQRN--IAGGFLVYGGIPARPMP 66

Query: 270  ELRPVVFNLTEFGGIGDGVTLNTKAFERAVIAISKFAKKGGAQLNVPAGNWLTAPFNLTS 449
             LRP+ FNLT+FGG+GDGVTLNT+AF+RAV A+SKF KKGGAQLNVP G WLTAPFNLTS
Sbjct: 67   MLRPMAFNLTDFGGVGDGVTLNTEAFKRAVSAVSKFGKKGGAQLNVPPGRWLTAPFNLTS 126

Query: 450  HMTLFLAQDAVILGVDDEKYWSLLPPLPSYGHGREHRGARYGSLIHGQHLKDVVITGHNG 629
            HMTLFLA+DAVILG+DDEKYW L+PPLPSYG+GREH G RYGSLIHGQHLKDVVITGHNG
Sbjct: 127  HMTLFLAEDAVILGIDDEKYWPLMPPLPSYGYGREHPGPRYGSLIHGQHLKDVVITGHNG 186

Query: 630  TINGQGRSWWKKYRQKSLNHTRGPLVQIMWSSDILISNITLCDSPFWTLHPYDCKNVTIR 809
            TINGQG++WWKKYRQK LNHTRGPLVQIM+SSDI+I+NITL DSPFWTLHPYDCKN+TI+
Sbjct: 187  TINGQGQTWWKKYRQKRLNHTRGPLVQIMFSSDIVITNITLRDSPFWTLHPYDCKNITIK 246

Query: 810  HMTILAPISGAPNTDGIDPDSCENMIIEDCYISVGDDAIAIKSGWDQYGISYGRPSKNIL 989
             +TILAP+ GAPNTDGIDPDSCE+M+IEDCYISVGDDAIAIKSGWDQYGI+YGRPS NI+
Sbjct: 247  GVTILAPVFGAPNTDGIDPDSCEDMLIEDCYISVGDDAIAIKSGWDQYGIAYGRPSMNIM 306

Query: 990  IRNLVVRSMVSAGISIGSEMSGGVSNVTVENVLVWNSRRAVRIKTAIGRGGYVRHITYRN 1169
            IRNLVVRSMVSAGISIGSEMSGGVSNV VEN+L+W+SRR VRIKTA GRG YVR ITYRN
Sbjct: 307  IRNLVVRSMVSAGISIGSEMSGGVSNVMVENILIWDSRRGVRIKTARGRGAYVRQITYRN 366

Query: 1170 LTFENVRVGIVIKTDYNEHPDKGFNPKAIPVLQDISYSSIHGEGVRVPVRIEGSQEIPVR 1349
            +TFENVRVGIV+KTDYNEHPD G++P A+P+L+DIS++++HG+GVRVPVRI GS+EIPVR
Sbjct: 367  ITFENVRVGIVMKTDYNEHPDDGYDPMALPILRDISFTTVHGQGVRVPVRIHGSEEIPVR 426

Query: 1350 NVTFHDMSVGLTYKKKHIFQCAYVQGRVIGTIFPAPCENLD 1472
            NVTF DMSVGLTYKKKHIFQCA+VQGRVIGTI+PAPCENLD
Sbjct: 427  NVTFQDMSVGLTYKKKHIFQCAFVQGRVIGTIYPAPCENLD 467