BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018659
         (1829 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAF01687.1| hypothetical protein [Arabidopsis thaliana]           300   7e-79
dbj|BAA97290.1| non-LTR retroelement reverse transcriptase-like ...   300   7e-79
gb|AAF98181.1|AC000107_4 F17F8.5 [Arabidopsis thaliana]               296   9e-78
gb|AAC28221.1| similar to reverse transcriptases (PFam: rvt.hmm,...   295   3e-77
gb|AAG50806.1|AC079281_8 unknown protein [Arabidopsis thaliana]       291   5e-76

>dbj|BAF01687.1| hypothetical protein [Arabidopsis thaliana]
          Length = 1072

 Score =  300 bits (769), Expect = 7e-79
 Identities = 181/593 (30%), Positives = 286/593 (48%), Gaps = 16/593 (2%)
 Frame = -3

Query: 1827 ISWIRHCLSSTMISVKINGSLEGYFKAKVGLRQGDPLSPYLFVIAMEAFSAIINKATDLH 1648
            I+WI  C+++   ++ +NG+  G+F++  GLRQGDPLSPYLFV+AME FS ++    D  
Sbjct: 480  INWIHQCITTPSFTISVNGATGGFFRSTKGLRQGDPLSPYLFVLAMEVFSKLLYSRYDSG 539

Query: 1647 QFRFHKDATDPKVSHLFFADDVMLFCRGDADSINVLLNATDQFAKYSGLRPNPSKSSCYF 1468
               +H  A D  +SHL FADDVM+F  G + S++ +    D FA +SGL+ N  KS  + 
Sbjct: 540  YIHYHPKAGDLSISHLMFADDVMIFFDGGSSSMHGICETLDDFADWSGLKVNKDKSQLFQ 599

Query: 1467 ANVPLCIVQRVLKRC-RFSWGSLPVKFLGLPLLSSSPTDRDCEPLITRICNRIQSWTARF 1291
            A + L   +R+      F  G+ P+++LGLPL+       D  PL+ ++  R++SW ++ 
Sbjct: 600  AGLDLS--ERITSAAYGFPAGTFPIRYLGLPLMCRKLRIADYGPLLEKLSARLRSWVSKA 657

Query: 1290 LSFAGRIQLIKSILYSIQEFWAMYLFLPVKVLKTLQSIFARFLWSGKRDGKCNYKVAWQE 1111
            LSFAGR QLI S+++ +  FW     LP   +K ++S+ ++FLW+G  DG+ + KV+W +
Sbjct: 658  LSFAGRTQLISSVIFGLINFWMSTFLLPKGCIKKIESLCSKFLWAGSIDGRKSSKVSWVD 717

Query: 1110 CTKPICCGGLGIKDLRLWNKAAVQYQLWRVVSPRSSSLWTSWFRGCFLKNKAFWTMKKPS 931
            C  P   GGLG +    WNK  +   +W V+  R +SLW  W R   L + +FW +    
Sbjct: 718  CCLPKSEGGLGFRSFGEWNKTLLLRLIW-VLFDRDTSLWAQWQRHHRLGHASFWQVNALQ 776

Query: 930  SCSWCISKILNARYEAMSHVNYKIGKGDNTLLWHDPWLNGRPVINVLXXXXXXXXXXXSH 751
            +  W    +LN R  A   +  K+G G     W D W +  P+I  L             
Sbjct: 777  TDPWTWKMLLNLRPLAEKFIKAKVGNGGTVSFWFDCWTSLGPLIKYLGDVGSRPLRIPFS 836

Query: 750  ALVSSIIKDGSWSVGPSNHALAIE--FRHLLS---GVRLHSNDTVLW--EDKPSSQVSIS 592
            A V+  I    W + P + +L  +    HL S      L  +D+  W  +D      S +
Sbjct: 837  AKVADAIDGSGWRL-PLSRSLTADSILSHLASLPPPSPLMVSDSYSWCVDDVDCQGFSAA 895

Query: 591  FIYNSGRPLHTCVPWSGFIWFPGAVPRFSFCCWXXXXXXXXXXXXXLSYGLLDEAICGLC 412
              +   RP      W+  +WF GAVP+ +F  W             +S+GL+  A C LC
Sbjct: 896  KTWEVLRPRRPVKRWARSVWFKGAVPKHAFNFWTAQLNRLPTRQRLVSWGLVSSAECCLC 955

Query: 411  CQYDEDERHLFFSCSFASRI-----ISECXXXXXXXXXXXXXHCDTLSAGGFEFQYIRLY 247
                E   HL   C F+S++     +  C                  S         ++ 
Sbjct: 956  SFDTETRDHLLLLCDFSSQVWRMVFLRLCPRQRLLCTWAELLSWTRQSTAAAPSLLRKVV 1015

Query: 246  VTTAIYYIWQQRNCRLWNPSQALTVDATIQLIKKTVRQIVFG---CNRFRKLL 97
                +Y +W+QRN  L + S  ++     +L+ + +R ++       R+R+LL
Sbjct: 1016 AQLVVYNLWRQRNLVL-HSSLRVSCSVVFRLVDRELRNVILSRRHKRRWRELL 1067


>dbj|BAA97290.1| non-LTR retroelement reverse transcriptase-like [Arabidopsis
            thaliana]
          Length = 1072

 Score =  300 bits (769), Expect = 7e-79
 Identities = 181/593 (30%), Positives = 286/593 (48%), Gaps = 16/593 (2%)
 Frame = -3

Query: 1827 ISWIRHCLSSTMISVKINGSLEGYFKAKVGLRQGDPLSPYLFVIAMEAFSAIINKATDLH 1648
            I+WI  C+++   ++ +NG+  G+F++  GLRQGDPLSPYLFV+AME FS ++    D  
Sbjct: 480  INWIHQCITTPSFTISVNGATGGFFRSTKGLRQGDPLSPYLFVLAMEVFSKLLYSRYDSG 539

Query: 1647 QFRFHKDATDPKVSHLFFADDVMLFCRGDADSINVLLNATDQFAKYSGLRPNPSKSSCYF 1468
               +H  A D  +SHL FADDVM+F  G + S++ +    D FA +SGL+ N  KS  + 
Sbjct: 540  YIHYHPKAGDLSISHLMFADDVMIFFDGGSSSMHGICETLDDFADWSGLKVNKDKSQLFQ 599

Query: 1467 ANVPLCIVQRVLKRC-RFSWGSLPVKFLGLPLLSSSPTDRDCEPLITRICNRIQSWTARF 1291
            A + L   +R+      F  G+ P+++LGLPL+       D  PL+ ++  R++SW ++ 
Sbjct: 600  AGLDLS--ERITSAAYGFPAGTFPIRYLGLPLMCRKLRIADYGPLLEKLSARLRSWVSKA 657

Query: 1290 LSFAGRIQLIKSILYSIQEFWAMYLFLPVKVLKTLQSIFARFLWSGKRDGKCNYKVAWQE 1111
            LSFAGR QLI S+++ +  FW     LP   +K ++S+ ++FLW+G  DG+ + KV+W +
Sbjct: 658  LSFAGRTQLISSVIFGLINFWMSTFLLPKGCIKKIESLCSKFLWAGSIDGRKSSKVSWVD 717

Query: 1110 CTKPICCGGLGIKDLRLWNKAAVQYQLWRVVSPRSSSLWTSWFRGCFLKNKAFWTMKKPS 931
            C  P   GGLG +    WNK  +   +W V+  R +SLW  W R   L + +FW +    
Sbjct: 718  CCLPKSEGGLGFRSFGEWNKTLLLRLIW-VLFDRDTSLWAQWQRHHRLGHASFWQVNALQ 776

Query: 930  SCSWCISKILNARYEAMSHVNYKIGKGDNTLLWHDPWLNGRPVINVLXXXXXXXXXXXSH 751
            +  W    +LN R  A   +  K+G G     W D W +  P+I  L             
Sbjct: 777  TDPWTWKMLLNLRPLAEKFIKAKVGNGGTVSFWFDCWTSLGPLIKYLGDVGSRPLRIPFS 836

Query: 750  ALVSSIIKDGSWSVGPSNHALAIE--FRHLLS---GVRLHSNDTVLW--EDKPSSQVSIS 592
            A V+  I    W + P + +L  +    HL S      L  +D+  W  +D      S +
Sbjct: 837  AKVADAIDGSGWRL-PLSRSLTADSILSHLASLPPPSPLMVSDSYSWCVDDVDCQGFSAA 895

Query: 591  FIYNSGRPLHTCVPWSGFIWFPGAVPRFSFCCWXXXXXXXXXXXXXLSYGLLDEAICGLC 412
              +   RP      W+  +WF GAVP+ +F  W             +S+GL+  A C LC
Sbjct: 896  KTWEVLRPRRPVKRWAKSVWFKGAVPKHAFNFWTAQLNRLPTRQRLVSWGLVSSAECCLC 955

Query: 411  CQYDEDERHLFFSCSFASRI-----ISECXXXXXXXXXXXXXHCDTLSAGGFEFQYIRLY 247
                E   HL   C F+S++     +  C                  S         ++ 
Sbjct: 956  SFDTETRDHLLLLCDFSSQVWRMVFLRLCPRQRLLCTWAELLSWTRQSTAAAPSLLRKVV 1015

Query: 246  VTTAIYYIWQQRNCRLWNPSQALTVDATIQLIKKTVRQIVFG---CNRFRKLL 97
                +Y +W+QRN  L + S  ++     +L+ + +R ++       R+R+LL
Sbjct: 1016 AQLVVYNLWRQRNLVL-HSSLRVSCSVVFRLVDRELRNVILSRRHKRRWRELL 1067


>gb|AAF98181.1|AC000107_4 F17F8.5 [Arabidopsis thaliana]
          Length = 872

 Score =  296 bits (759), Expect = 9e-78
 Identities = 192/586 (32%), Positives = 288/586 (49%), Gaps = 20/586 (3%)
 Frame = -3

Query: 1827 ISWIRHCLSSTMISVKINGSLEGYFKAKVGLRQGDPLSPYLFVIAMEAFSAIINKATDLH 1648
            I WI  C+++   SV++NG L GYF++K GLRQG  LSPYLFVI M+  S +++KA  + 
Sbjct: 273  IHWINLCITTASFSVQVNGDLVGYFQSKRGLRQGCSLSPYLFVICMDVLSKMLDKAAGVR 332

Query: 1647 QFRFHKDATDPKVSHLFFADDVMLFCRGDADSINVLLNATDQFAKYSGLRPNPSKSSCYF 1468
            +F FH       ++HL FADD+M+   G   SI  +L   D+F K SGLR +  KS+ Y 
Sbjct: 333  KFGFHPKCQRLGLTHLSFADDLMVLSDGKTRSIEGILEVFDEFCKRSGLRISLEKSTLYM 392

Query: 1467 ANVPLCIVQRVLKRCRFSWGSLPVKFLGLPLLSSSPTDRDCEPLITRICNRIQSWTARFL 1288
            A V   I Q +  +  F  G LPV++LGLPL++   T  D  PL+ +I  RI +WT RF 
Sbjct: 393  AGVSPIIKQEIAAKFLFDVGQLPVRYLGLPLVTKRLTSADYSPLLEQIKKRIATWTFRFF 452

Query: 1287 SFAGRIQLIKSILYSIQEFWAMYLFLPVKVLKTLQSIFARFLWSGKRDGKCNYKVAWQEC 1108
            SFAGR  LIKS+L+SI  FW     LP + ++ +  + + FLWSG        K++W   
Sbjct: 453  SFAGRFNLIKSVLWSICNFWLAAFRLPRQCIREIDKLCSSFLWSGSEMSSHKAKISWDIV 512

Query: 1107 TKPICCGGLGIKDLRLWNKAAVQYQLWRVVSPRSSSLWTSWFRGCFLKNKAFWTMKKPSS 928
             KP   GGLG+++L+  N  +    +WR++S  S+SLWT W     ++ K+ W++K+ +S
Sbjct: 513  CKPKAEGGLGLRNLKEANDVSCLKLVWRIIS-NSNSLWTKWVAEYLIRKKSIWSLKQSTS 571

Query: 927  C-SWCISKILNARYEAMSHVNYKIGKGDNTLLWHDPWLNGRPVINVLXXXXXXXXXXXSH 751
              SW   KIL  R  A S    ++G G++   W+D W     +I+ +             
Sbjct: 572  MGSWIWRKILKIRDVAKSFSRVEVGNGESASFWYDHWSAHGRLIDTVGDKGTIDLGIPRE 631

Query: 750  ALVSSIIKDGSW---SVGPSNHALAIEFRHLLSGVRLH---SNDTVLWEDKP---SSQVS 598
            A V+      +W   S      +L  E   +++  R+H   + DTVLW  K        S
Sbjct: 632  ASVAD-----AWTRRSRRRHRTSLLNEIEEMMAYQRIHHSDAEDTVLWRGKNDVFKPHFS 686

Query: 597  ISFIYNSGRPLHTCVPWSGFIWFPGAVPRFSFCCWXXXXXXXXXXXXXLSYGLLDEAI-- 424
                ++  +   + V W   +WF  A P+++ C W             L +         
Sbjct: 687  TRDTWHLIKATSSTVSWHKGVWFRHATPKYALCTWLAIHNRLPTGDRMLKWNSSGSVSGN 746

Query: 423  CGLCCQYDEDERHLFFSCSFASRIISECXXXXXXXXXXXXXHCDTLSAGGFEFQ-----Y 259
            C LC    +   HLFFSCS+AS + +                   L+     FQ     +
Sbjct: 747  CVLCTNNSKTLEHLFFSCSYASTVWA-ALAKGIWKTRYSTRWSHLLTHISTHFQDRVEGF 805

Query: 258  IRLYVTTA-IYYIWQQRNCRLWN--PSQALTVDATIQLIKKTVRQI 130
            +  Y+  A IY++W++RN R  +  P+   TV   I   K+T  QI
Sbjct: 806  LTRYIFQATIYHVWRERNGRRHDAAPNTPATVIGWID--KQTRNQI 849


>gb|AAC28221.1| similar to reverse transcriptases (PFam: rvt.hmm, score: 60.13)
            [Arabidopsis thaliana]
          Length = 1164

 Score =  295 bits (755), Expect = 3e-77
 Identities = 165/495 (33%), Positives = 249/495 (50%), Gaps = 7/495 (1%)
 Frame = -3

Query: 1821 WIRHCLSSTMISVKINGSLEGYFKAKVGLRQGDPLSPYLFVIAMEAFSAIINKATDLHQF 1642
            WI  CLS+   SV +NG   G+F +  GLRQGDP+SPYLFV+AME FS ++         
Sbjct: 519  WILECLSTASFSVILNGHSAGHFWSSKGLRQGDPMSPYLFVLAMEVFSGLLQSRYTSGYI 578

Query: 1641 RFHKDATDPKVSHLFFADDVMLFCRGDADSINVLLNATDQFAKYSGLRPNPSKSSCYFAN 1462
             +H   +  ++SHL FADDVM+F  G + S++ ++ + + FA +SGL  N +K+  Y A 
Sbjct: 579  AYHPKTSQLEISHLMFADDVMIFFDGKSSSLHGIVESLEDFAGWSGLLMNTNKTQLYHAG 638

Query: 1461 VPLCIVQRVLKRCRFSWGSLPVKFLGLPLLSSSPTDRDCEPLITRICNRIQSWTARFLSF 1282
            +       +     F  GSLPV++LGLPL+S   T  +  PLI +I  R  SW  R LSF
Sbjct: 639  LSQSESDSMASY-GFKLGSLPVRYLGLPLMSRKLTIAEYAPLIEKITARFNSWVVRLLSF 697

Query: 1281 AGRIQLIKSILYSIQEFWAMYLFLPVKVLKTLQSIFARFLWSGKRDGKCNYKVAWQECTK 1102
            AGR+QL+ S++  I  FW     LP+  +K ++S+ +RFLWS + D K   KVAW +   
Sbjct: 698  AGRVQLLASVISGIVNFWISSFILPLGCIKKIESLCSRFLWSSRIDKKGIAKVAWSQVCL 757

Query: 1101 PICCGGLGIKDLRLWNKAAVQYQLWRVVSPRSSSLWTSWFRGCFL-KNKAFWTMKKPSSC 925
            P   GG+G++   + N+      +W + S  S SLW +W +   L K+ +FW   +    
Sbjct: 758  PKAEGGIGLRRFAVSNRTLYLRMIWLLFS-NSGSLWVAWHKQHSLGKSTSFWNQPEKPHD 816

Query: 924  SWCISKILNARYEAMSHVNYKIGKGDNTLLWHDPWLNGRPVINVLXXXXXXXXXXXSHAL 745
            SW    +L  R  A   +   +G G +   W D W    P+I  L            +A 
Sbjct: 817  SWNWKCLLRLRVVAERFIRCNVGNGRDASFWFDNWTPFGPLIKFLGNEGPRDLRVHLNAK 876

Query: 744  VSSIIKDGSWSVGPSNHALAIEFRHLLSGVRLHSN----DTVLW--EDKPSSQVSISFIY 583
            +S +     WS+       A+     L+ + + S+    D+  W  ++K     S +  +
Sbjct: 877  ISDVCTSEGWSIADPRSDQALSLHTHLTNISMPSDAQDLDSYDWVVDNKVCQGFSAAATW 936

Query: 582  NSGRPLHTCVPWSGFIWFPGAVPRFSFCCWXXXXXXXXXXXXXLSYGLLDEAICGLCCQY 403
            ++ RP    VPW+  +WF GA P+ +F  W              S+G+  +  CGLC  +
Sbjct: 937  SALRPSSAPVPWARAVWFKGATPKHAFHLWTAHLDRLPTKVRLASWGMQIDTTCGLCSLH 996

Query: 402  DEDERHLFFSCSFAS 358
             E   HLF SC FA+
Sbjct: 997  PETRDHLFLSCDFAN 1011


>gb|AAG50806.1|AC079281_8 unknown protein [Arabidopsis thaliana]
          Length = 1213

 Score =  291 bits (744), Expect = 5e-76
 Identities = 186/591 (31%), Positives = 279/591 (47%), Gaps = 12/591 (2%)
 Frame = -3

Query: 1827 ISWIRHCLSSTMISVKINGSLEGYFKAKVGLRQGDPLSPYLFVIAMEAFSAIINKATDLH 1648
            I+WI  C+S+   +V ING   G+FK+  GLRQGDPLSPYLFV+AMEAFS +++   +  
Sbjct: 620  INWISQCISTPTFTVSINGGNGGFFKSTKGLRQGDPLSPYLFVLAMEAFSNLLHSRYESG 679

Query: 1647 QFRFHKDATDPKVSHLFFADDVMLFCRGDADSINVLLNATDQFAKYSGLRPNPSKSSCYF 1468
               +H  A++  +SHL FADDVM+F  G + S++ +    D FA +SGL+ N  KS  Y 
Sbjct: 680  LIHYHPKASNLSISHLMFADDVMIFFDGGSFSLHGICETLDDFASWSGLKVNKDKSHLYL 739

Query: 1467 ANVPLCIVQRVLKRCRFSWGSLPVKFLGLPLLSSSPTDRDCEPLITRICNRIQSWTARFL 1288
            A +   +         F  G+LP+++LGLPL++      + EPL+ +I  R +SW  + L
Sbjct: 740  AGLNQ-LESNANAAYGFPIGTLPIRYLGLPLMNRKLRIAEYEPLLEKITARFRSWVNKCL 798

Query: 1287 SFAGRIQLIKSILYSIQEFWAMYLFLPVKVLKTLQSIFARFLWSGKRDGKCNYKVAWQEC 1108
            SFAGRIQLI S+++    FW     LP   +K ++S+ +RFLWSG  +     KV+W   
Sbjct: 799  SFAGRIQLISSVIFGSINFWMSTFLLPKGCIKRIESLCSRFLWSGNIEQAKGIKVSWAAL 858

Query: 1107 TKPICCGGLGIKDLRLWNKAAVQYQLWRVVSPRSSSLWTSWFRGCFLKNKAFWTMKKPSS 928
              P   GGLG++ L  WNK      +WR+   +  SLW  W     L   +FW ++   S
Sbjct: 859  CLPKSEGGLGLRRLLEWNKTLSMRLIWRLFVAK-DSLWADWQHLHHLSRGSFWAVEGGQS 917

Query: 927  CSWCISKILNARYEAMSHVNYKIGKGDNTLLWHDPWLNGRPVINVLXXXXXXXXXXXSHA 748
             SW   ++L+ R  A   +  K+G G     W+D W +  P+  ++             A
Sbjct: 918  DSWTWKRLLSLRPLAHQFLVCKVGNGLKADYWYDNWTSLGPLFRIIGDIGPSSLRVPLLA 977

Query: 747  LVSSIIKDGSWSVGPSNHALAIEFRHLLSGVRLHSN-----DTVLWEDKP--SSQVSISF 589
             V+S   +  W +  S  A A      L  V + S      D   W          S + 
Sbjct: 978  KVASAFSEDGWRLPVSRSAPAKGIHDHLCTVPVPSTAQEDVDRYEWSVNGFLCQGFSAAK 1037

Query: 588  IYNSGRPLHTCVPWSGFIWFPGAVPRFSFCCWXXXXXXXXXXXXXLSYGLLDEAICGLCC 409
             + + RP  T   W+  IWF GAVP+++F  W              S+G +    C LC 
Sbjct: 1038 TWEAIRPKATVKSWASSIWFKGAVPKYAFNMWVSHLNRLLTRQRLASWGHIQSDACVLCS 1097

Query: 408  QYDEDERHLFFSCSFASRIISECXXXXXXXXXXXXXHCDTLS----AGGFEFQYIRLYVT 241
               E   HL   C F++++                   + LS    +       +R  V+
Sbjct: 1098 FASESRDHLLLICEFSAQVWRLVFRRICPRQRLFSSWSELLSWVRQSSPEAPPLLRKIVS 1157

Query: 240  -TAIYYIWQQRNCRLWNPSQALTVDATIQLIKKTVRQIVFGCNRFRKLLKK 91
               +Y +W+QRN  L N S  L      +L+ + +R I+    R RK  +K
Sbjct: 1158 QVVVYNLWRQRNNLLHN-SLRLAPAVIFKLVDREIRNII-SSRRLRKRWRK 1206