BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018543
         (1196 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002303465.1| predicted protein [Populus trichocarpa] gi|2...   283   e-145
ref|XP_002518706.1| triacylglycerol lipase, putative [Ricinus co...   271   e-140
gb|ACJ85535.1| unknown [Medicago truncatula]                          266   e-137
ref|XP_003607601.1| Feruloyl esterase A [Medicago truncatula] gi...   262   e-135
ref|NP_181797.1| alpha/beta-hydrolase domain-containing protein ...   244   e-128

>ref|XP_002303465.1| predicted protein [Populus trichocarpa] gi|222840897|gb|EEE78444.1|
            predicted protein [Populus trichocarpa]
          Length = 411

 Score =  283 bits (723), Expect(2) = e-145
 Identities = 135/190 (71%), Positives = 160/190 (84%)
 Frame = +1

Query: 625  KLSCRAQLMNKMKELLEIYKSENVSITFTGHSLGASLSVVSAFDLAENGVTNIPISAFIF 804
            KLS RAQL+  +KEL E YK +++SI FTGHSLGASLS++SAFDL ENG+T+IP+SAF+F
Sbjct: 205  KLSARAQLLAHIKELRERYKDDDLSIIFTGHSLGASLSILSAFDLVENGITDIPVSAFVF 264

Query: 805  GSPQVGNKVFNNKLKSFTNVKILHIRNKIDLIPLYPSLLLGYVNSGIELLIDNRKSPSLK 984
            GSPQVGNK FN +   + N+K+LHI+NKID+IP YP  L+GYV +GIE  ID RKSPSLK
Sbjct: 265  GSPQVGNKEFNERFNKYPNLKVLHIKNKIDVIPHYPGRLMGYVYTGIEFEIDTRKSPSLK 324

Query: 985  DSNNPSDWHNLQAMLHTVDGWNGDKGVFDLKVKRSLALVNKSSEVLKDEYLIPGSWWVEK 1164
            DS NPSDWHNLQAMLH V GWNG++  F+LKVKRSLALVNKSSE LKDE L+PG WWVEK
Sbjct: 325  DSKNPSDWHNLQAMLHIVAGWNGEEQEFELKVKRSLALVNKSSEFLKDECLVPGIWWVEK 384

Query: 1165 NKGMVIDENG 1194
            NKGMV +E+G
Sbjct: 385  NKGMVRNEDG 394



 Score =  260 bits (665), Expect(2) = e-145
 Identities = 122/186 (65%), Positives = 150/186 (80%), Gaps = 1/186 (0%)
 Frame = +3

Query: 30  EPSWLELQGTKDWQGLLNPLDLSLRHLILRCGDFCQATYDAFNNDQNSKYCGSSRYGKKD 209
           E +W E+ G+++W  LL+PLDLSLR LILRCGDFCQATYDAFNNDQNS+YCG+SRYGK++
Sbjct: 6   EATWPEILGSRNWDNLLDPLDLSLRKLILRCGDFCQATYDAFNNDQNSRYCGTSRYGKRN 65

Query: 210 FFQKVMLTNASQYQVSCFLYATAKVTTGQAFLLHSRSREAWDRESNWIGYIATTTDEYSA 389
           FF KVML N   YQVS FLYATA+V+  +AFLLHS SR++WDRE+NWIGYIA T+DE + 
Sbjct: 66  FFHKVMLDNPENYQVSSFLYATARVSLPEAFLLHSLSRDSWDRETNWIGYIAVTSDEQTK 125

Query: 390 TLGRREIYVAWRGTSRDYEWINVFDPKPASAKPLLNPKNQQLTSGTASSSSDEDDD-LPK 566
           TLGRREIY+A+RGT+R+YEW+++   K  SAKPLL           +SSSSD+DDD +PK
Sbjct: 126 TLGRREIYIAFRGTTRNYEWVDILGAKLKSAKPLLRGATSTTHDQESSSSSDDDDDKVPK 185

Query: 567 VMQGWL 584
           VM GWL
Sbjct: 186 VMLGWL 191


>ref|XP_002518706.1| triacylglycerol lipase, putative [Ricinus communis]
            gi|223542087|gb|EEF43631.1| triacylglycerol lipase,
            putative [Ricinus communis]
          Length = 417

 Score =  271 bits (692), Expect(2) = e-140
 Identities = 129/190 (67%), Positives = 155/190 (81%)
 Frame = +1

Query: 625  KLSCRAQLMNKMKELLEIYKSENVSITFTGHSLGASLSVVSAFDLAENGVTNIPISAFIF 804
            KLS R QL++K+ EL+E YK +N+SI  TGHSLGASLS+++AFDL ENGV +IP++A +F
Sbjct: 211  KLSLRKQLLSKLNELIEQYKDDNLSILLTGHSLGASLSILAAFDLVENGVADIPVAALVF 270

Query: 805  GSPQVGNKVFNNKLKSFTNVKILHIRNKIDLIPLYPSLLLGYVNSGIELLIDNRKSPSLK 984
            GSPQVGNK FN++ K  +N+K+LHI+N+ID IP YP  LLGY  +G EL ID RKSPSLK
Sbjct: 271  GSPQVGNKAFNDRFKKHSNLKVLHIKNQIDAIPHYPGRLLGYEYTGTELEIDTRKSPSLK 330

Query: 985  DSNNPSDWHNLQAMLHTVDGWNGDKGVFDLKVKRSLALVNKSSEVLKDEYLIPGSWWVEK 1164
             S NPSDWHNLQAMLH V GWNGDK  F+LKVKRSLALVNKS   LKDE L+PGSWWVEK
Sbjct: 331  GSKNPSDWHNLQAMLHIVAGWNGDKEPFELKVKRSLALVNKSCAFLKDECLVPGSWWVEK 390

Query: 1165 NKGMVIDENG 1194
            N+GMV  ++G
Sbjct: 391  NRGMVRGDDG 400



 Score =  254 bits (648), Expect(2) = e-140
 Identities = 120/195 (61%), Positives = 151/195 (77%), Gaps = 5/195 (2%)
 Frame = +3

Query: 15  TPTEKEPSWLELQGTKDWQGLLNPLDLSLRHLILRCGDFCQATYDAFNNDQNSKYCGSSR 194
           T  + + +W EL G  +W+ LL+PLDL+LR LILRCGD CQATYD+FNNDQNSKYCG+SR
Sbjct: 3   TINQPQATWSELLGQNNWENLLDPLDLNLRKLILRCGDLCQATYDSFNNDQNSKYCGASR 62

Query: 195 YGKKDFFQKVMLTNASQYQVSCFLYATAKVTTGQAFLLHSRSREAWDRESNWIGYIATTT 374
           YGK +FFQKVM   A  YQVS FLY TA+V+  +AF+LHS+SRE+WDRESNWIGYIA TT
Sbjct: 63  YGKHNFFQKVMFEEAENYQVSSFLYGTARVSLPEAFILHSQSRESWDRESNWIGYIAVTT 122

Query: 375 DEYSATLGRREIYVAWRGTSRDYEWINVFDPKPASAKPLL----NPKNQQLTSGTASSSS 542
           DEYS   GRR+IY+A RGT+R+YEW+N+   K  SA  LL    N  +Q++ +  +SS S
Sbjct: 123 DEYSKATGRRDIYIALRGTTRNYEWVNILGAKLQSANHLLQGSSNNLDQEMKNSASSSDS 182

Query: 543 DEDDD-LPKVMQGWL 584
           D+D++ +PKVMQGWL
Sbjct: 183 DDDNEKVPKVMQGWL 197


>gb|ACJ85535.1| unknown [Medicago truncatula]
          Length = 435

 Score =  266 bits (680), Expect(2) = e-137
 Identities = 127/185 (68%), Positives = 149/185 (80%)
 Frame = +1

Query: 625  KLSCRAQLMNKMKELLEIYKSENVSITFTGHSLGASLSVVSAFDLAENGVTNIPISAFIF 804
            K S R Q++N +K LL  YK+EN S+   GHSLGASLS+VSAFDL ENGVT+IP++AF+F
Sbjct: 236  KTSVRTQVLNIVKSLLNKYKNENPSVVLVGHSLGASLSIVSAFDLVENGVTDIPVTAFVF 295

Query: 805  GSPQVGNKVFNNKLKSFTNVKILHIRNKIDLIPLYPSLLLGYVNSGIELLIDNRKSPSLK 984
            GSPQVGNK FN++ K F N+K+LH+RN IDLIP YP  LLGY  +G EL+ID RKS SLK
Sbjct: 296  GSPQVGNKAFNDRFKKFQNLKVLHVRNVIDLIPHYPGKLLGYEYTGTELVIDTRKSTSLK 355

Query: 985  DSNNPSDWHNLQAMLHTVDGWNGDKGVFDLKVKRSLALVNKSSEVLKDEYLIPGSWWVEK 1164
            DS NPSDWHNLQAMLH V GWNG  G F++KVKRSLALVNKS + LK+E  +PGSWWV K
Sbjct: 356  DSKNPSDWHNLQAMLHIVAGWNGSDGEFEVKVKRSLALVNKSCDYLKEECHVPGSWWVAK 415

Query: 1165 NKGMV 1179
            NKGMV
Sbjct: 416  NKGMV 420



 Score =  249 bits (635), Expect(2) = e-137
 Identities = 119/202 (58%), Positives = 145/202 (71%), Gaps = 9/202 (4%)
 Frame = +3

Query: 6   ATSTPTEKEPSWLELQGTKDWQGLLNPLDLSLRHLILRCGDFCQATYDAFNNDQNSKYCG 185
           +T   T  + +W +L G  +W+ LLNPLDL+LR+LILRCGDF Q TYD+FNNDQNS YCG
Sbjct: 21  STQPTTINQETWNQLLGNNNWETLLNPLDLNLRNLILRCGDFIQTTYDSFNNDQNSIYCG 80

Query: 186 SSRYGKKDFFQKVMLTNASQYQVSCFLYATAKVTTGQAFLLHSRSREAWDRESNWIGYIA 365
           SSRYGK  FF KVML N + Y V  FLYATA+V+  +AF+LHS SRE+WDRESNWIGYIA
Sbjct: 81  SSRYGKTSFFNKVMLENPTHYTVVSFLYATARVSVPEAFILHSLSRESWDRESNWIGYIA 140

Query: 366 TTTDEYSATLGRREIYVAWRGTSRDYEWINVFDPKPASAKPLLN---------PKNQQLT 518
            ++DE S  LGRREIYV WRGT+RD EWINVF   P SA  LL+         P N    
Sbjct: 141 VSSDERSRELGRREIYVVWRGTTRDLEWINVFGAAPESASGLLSAKSLREFNLPNNNNKD 200

Query: 519 SGTASSSSDEDDDLPKVMQGWL 584
            G++SS  ++D  +PK+M+GWL
Sbjct: 201 DGSSSSDDEDDKSVPKIMKGWL 222


>ref|XP_003607601.1| Feruloyl esterase A [Medicago truncatula] gi|355508656|gb|AES89798.1|
            Feruloyl esterase A [Medicago truncatula]
          Length = 442

 Score =  262 bits (669), Expect(2) = e-135
 Identities = 126/189 (66%), Positives = 149/189 (78%)
 Frame = +1

Query: 625  KLSCRAQLMNKMKELLEIYKSENVSITFTGHSLGASLSVVSAFDLAENGVTNIPISAFIF 804
            K S R Q++N +K LL  YK+EN S+   GHSLGASLS+VSAFDL ENGVT+IP++AF+F
Sbjct: 236  KTSVRTQVLNIVKSLLNKYKNENPSVVLVGHSLGASLSIVSAFDLVENGVTDIPVTAFVF 295

Query: 805  GSPQVGNKVFNNKLKSFTNVKILHIRNKIDLIPLYPSLLLGYVNSGIELLIDNRKSPSLK 984
            GSPQVGNK FN++ K F N+K+LH+RN IDLIP YP  LLGY  +G EL+ID RKS SLK
Sbjct: 296  GSPQVGNKAFNDRFKKFQNLKVLHVRNVIDLIPHYPGKLLGYEYTGTELVIDTRKSTSLK 355

Query: 985  DSNNPSDWHNLQAMLHTVDGWNGDKGVFDLKVKRSLALVNKSSEVLKDEYLIPGSWWVEK 1164
            DS NPSDWHNLQAMLH V GWNG  G F++KVKRSLALVNKS + LK+E  +P SWWV  
Sbjct: 356  DSKNPSDWHNLQAMLHIVAGWNGSDGEFEVKVKRSLALVNKSCDYLKEECHVPASWWVAT 415

Query: 1165 NKGMVIDEN 1191
            NKGMV  E+
Sbjct: 416  NKGMVRRED 424



 Score =  249 bits (635), Expect(2) = e-135
 Identities = 119/202 (58%), Positives = 145/202 (71%), Gaps = 9/202 (4%)
 Frame = +3

Query: 6   ATSTPTEKEPSWLELQGTKDWQGLLNPLDLSLRHLILRCGDFCQATYDAFNNDQNSKYCG 185
           +T   T  + +W +L G  +W+ LLNPLDL+LR+LILRCGDF Q TYD+FNNDQNS YCG
Sbjct: 21  STQPTTINQETWNQLLGNNNWETLLNPLDLNLRNLILRCGDFIQTTYDSFNNDQNSIYCG 80

Query: 186 SSRYGKKDFFQKVMLTNASQYQVSCFLYATAKVTTGQAFLLHSRSREAWDRESNWIGYIA 365
           SSRYGK  FF KVML N + Y V  FLYATA+V+  +AF+LHS SRE+WDRESNWIGYIA
Sbjct: 81  SSRYGKTSFFNKVMLENPTHYTVVSFLYATARVSVPEAFILHSLSRESWDRESNWIGYIA 140

Query: 366 TTTDEYSATLGRREIYVAWRGTSRDYEWINVFDPKPASAKPLLN---------PKNQQLT 518
            ++DE S  LGRREIYV WRGT+RD EWINVF   P SA  LL+         P N    
Sbjct: 141 VSSDERSRELGRREIYVVWRGTTRDLEWINVFGAAPESASGLLSAKSLREFNLPNNNNKD 200

Query: 519 SGTASSSSDEDDDLPKVMQGWL 584
            G++SS  ++D  +PK+M+GWL
Sbjct: 201 DGSSSSDDEDDKSVPKIMKGWL 222


>ref|NP_181797.1| alpha/beta-hydrolase domain-containing protein [Arabidopsis
           thaliana] gi|75265946|sp|Q9SJI7.1|PLA20_ARATH RecName:
           Full=Phospholipase A1-IIdelta gi|4512683|gb|AAD21737.1|
           putative lipase [Arabidopsis thaliana]
           gi|15293129|gb|AAK93675.1| putative lipase [Arabidopsis
           thaliana] gi|19310713|gb|AAL85087.1| putative lipase
           [Arabidopsis thaliana] gi|20198071|gb|AAM15382.1|
           putative lipase [Arabidopsis thaliana]
           gi|330255062|gb|AEC10156.1| alpha/beta-hydrolase
           domain-containing protein [Arabidopsis thaliana]
          Length = 412

 Score =  244 bits (622), Expect(2) = e-128
 Identities = 122/198 (61%), Positives = 144/198 (72%), Gaps = 4/198 (2%)
 Frame = +3

Query: 3   MATSTPTEKEPSWLELQGTKDWQGLLNPLDLSLRHLILRCGDFCQATYDAFNNDQNSKYC 182
           MAT+T      SW EL G+K+W  +L+PLD SLR LILRCGDFCQATYDAF NDQNSKYC
Sbjct: 1   MATTTT-----SWEELLGSKNWDTILDPLDQSLRELILRCGDFCQATYDAFVNDQNSKYC 55

Query: 183 GSSRYGKKDFFQKVMLTNASQYQVSCFLYATAKVTTGQAFLLHSRSREAWDRESNWIGYI 362
           G+SRYGK  FF KVML NAS Y+V  FLYATA+V+  +  LL S+SR++WDRESNW GYI
Sbjct: 56  GASRYGKSSFFDKVMLENASDYEVVNFLYATARVSLPEGLLLQSQSRDSWDRESNWFGYI 115

Query: 363 ATTTDEYSATLGRREIYVAWRGTSRDYEWINVFDPKPASAKPLLNPKNQQ----LTSGTA 530
           A T+DE S  LGRREIY+A RGTSR+YEW+NV   +P SA PLL+   Q     +  GT 
Sbjct: 116 AVTSDERSKALGRREIYIALRGTSRNYEWVNVLGARPTSADPLLHGPEQDGSGGVVEGTT 175

Query: 531 SSSSDEDDDLPKVMQGWL 584
             S  ED++  KVM GWL
Sbjct: 176 FDSDSEDEEGCKVMLGWL 193



 Score =  243 bits (619), Expect(2) = e-128
 Identities = 118/191 (61%), Positives = 148/191 (77%), Gaps = 1/191 (0%)
 Frame = +1

Query: 625  KLSCRAQLMNKMKELLEIYKSENVSITFTGHSLGASLSVVSAFDLAENGVTN-IPISAFI 801
            KLS R+QL+ K+KELL  YK E  SI  TGHSLGA+ +V++A+D+AENG ++ +P++A +
Sbjct: 207  KLSLRSQLLAKIKELLLKYKDEKPSIVLTGHSLGATEAVLAAYDIAENGSSDDVPVTAIV 266

Query: 802  FGSPQVGNKVFNNKLKSFTNVKILHIRNKIDLIPLYPSLLLGYVNSGIELLIDNRKSPSL 981
            FG PQVGNK F +++ S  N+KILH+RN IDL+  YP  LLGYV+ GI  +ID +KSP L
Sbjct: 267  FGCPQVGNKEFRDEVMSHKNLKILHVRNTIDLLTRYPGGLLGYVDIGINFVIDTKKSPFL 326

Query: 982  KDSNNPSDWHNLQAMLHTVDGWNGDKGVFDLKVKRSLALVNKSSEVLKDEYLIPGSWWVE 1161
             DS NP DWHNLQAMLH V GWNG KG F L VKRS+ALVNKS E LK E L+PGSWWVE
Sbjct: 327  SDSRNPGDWHNLQAMLHVVAGWNGKKGEFKLMVKRSIALVNKSCEFLKAECLVPGSWWVE 386

Query: 1162 KNKGMVIDENG 1194
            KNKG++ +E+G
Sbjct: 387  KNKGLIKNEDG 397