BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00018537
         (538 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002285664.1| PREDICTED: trans-2,3-enoyl-CoA reductase [Vi...   219   3e-55
ref|XP_003518596.1| PREDICTED: trans-2,3-enoyl-CoA reductase-lik...   200   8e-50
ref|XP_003619177.1| 3-oxo-5-alpha-steroid 4-dehydrogenase [Medic...   199   2e-49
gb|ACU19102.1| unknown [Glycine max]                                  198   5e-49
ref|XP_002275776.1| PREDICTED: trans-2,3-enoyl-CoA reductase [Vi...   192   6e-48

>ref|XP_002285664.1| PREDICTED: trans-2,3-enoyl-CoA reductase [Vitis vinifera]
          Length = 259

 Score =  219 bits (557), Expect = 3e-55
 Identities = 104/161 (64%), Positives = 125/161 (77%)
 Frame = +2

Query: 2   ASVASLALLPDQGFRFTLVTSSLAIHFFKRLFEVTFIHKYSGSMPKDVAITISTSYLLSA 181
           A VAS AL PD+ FRF L+TS+L+IHFFKR+FEV F+HKYSG M  D AI IS SY +S 
Sbjct: 71  AGVASFALFPDESFRFLLLTSALSIHFFKRIFEVLFVHKYSGGMALDAAIVISLSYFIST 130

Query: 182 ISMIYSQYLTNEFTEPLIDLKYAGMAIFSVGIIGNFYHHYLLSKLRKDGKKEYIIPRGGL 361
            +MIY+Q+L+ +F EPL+DLKY GMA+F VGI+GN YHH  LSKLR  G++EY IP+GGL
Sbjct: 131 ATMIYAQHLSRDFPEPLMDLKYPGMALFLVGILGNLYHHCQLSKLRSKGEREYKIPKGGL 190

Query: 362 FNLVICPHYLFEILGFIGIACISQNVYALSYTVGVIGYLTG 484
           FNLV+CPHYLFEILGFIG + ISQ VYA +   G   YL G
Sbjct: 191 FNLVVCPHYLFEILGFIGFSFISQTVYAFALATGTALYLMG 231


>ref|XP_003518596.1| PREDICTED: trans-2,3-enoyl-CoA reductase-like [Glycine max]
          Length = 266

 Score =  200 bits (508), Expect(2) = 8e-50
 Identities = 98/161 (60%), Positives = 119/161 (73%)
 Frame = +2

Query: 2   ASVASLALLPDQGFRFTLVTSSLAIHFFKRLFEVTFIHKYSGSMPKDVAITISTSYLLSA 181
           A +AS  + P QG R TL+ S++ +HFFKR+FEV FIHKYSG+M  D AI I+ SY LS 
Sbjct: 78  AGLASFWIFPHQGLRSTLLQSAVTLHFFKRVFEVVFIHKYSGAMLLDSAIPITLSYFLST 137

Query: 182 ISMIYSQYLTNEFTEPLIDLKYAGMAIFSVGIIGNFYHHYLLSKLRKDGKKEYIIPRGGL 361
            +MIY+Q+LT    EP IDL Y G+ +F VGIIGNFYHHYLLS LR  G+KEY IP+GG+
Sbjct: 138 ATMIYAQHLTQGLPEPPIDLLYPGIVLFVVGIIGNFYHHYLLSNLRGKGEKEYKIPKGGM 197

Query: 362 FNLVICPHYLFEILGFIGIACISQNVYALSYTVGVIGYLTG 484
           F LVICPHYLFEI+ F G + ISQ +YA S+TVG   YL G
Sbjct: 198 FELVICPHYLFEIIEFYGFSFISQTLYAFSFTVGTTLYLLG 238



 Score = 22.3 bits (46), Expect(2) = 8e-50
 Identities = 7/11 (63%), Positives = 11/11 (100%)
 Frame = +1

Query: 481 RDVKALIPYIF 513
           +DVKA+IP++F
Sbjct: 256 KDVKAIIPFVF 266


>ref|XP_003619177.1| 3-oxo-5-alpha-steroid 4-dehydrogenase [Medicago truncatula]
           gi|355494192|gb|AES75395.1| 3-oxo-5-alpha-steroid
           4-dehydrogenase [Medicago truncatula]
          Length = 270

 Score =  199 bits (506), Expect = 2e-49
 Identities = 92/161 (57%), Positives = 119/161 (73%)
 Frame = +2

Query: 2   ASVASLALLPDQGFRFTLVTSSLAIHFFKRLFEVTFIHKYSGSMPKDVAITISTSYLLSA 181
           A  AS  + PD GFR  ++  ++  HFFKR+FEV F+HKYSGSM  + AI I+ SY LS+
Sbjct: 82  AGAASFLVFPDDGFRSLVLQGAVTFHFFKRVFEVLFVHKYSGSMALETAIPITLSYFLSS 141

Query: 182 ISMIYSQYLTNEFTEPLIDLKYAGMAIFSVGIIGNFYHHYLLSKLRKDGKKEYIIPRGGL 361
            ++IY+Q+LT+   EP IDL Y G+A+F VG+ GNFYHHYLLSKLR  G+KEY IP+GGL
Sbjct: 142 ATLIYAQHLTSNLPEPSIDLLYPGIALFLVGVTGNFYHHYLLSKLRGKGEKEYKIPKGGL 201

Query: 362 FNLVICPHYLFEILGFIGIACISQNVYALSYTVGVIGYLTG 484
           F+ VICPHYLFEI+GF G + ISQ +Y+ S+ +G   YL G
Sbjct: 202 FDFVICPHYLFEIIGFYGFSFISQTLYSFSFAIGTTFYLLG 242


>gb|ACU19102.1| unknown [Glycine max]
          Length = 266

 Score =  198 bits (503), Expect = 5e-49
 Identities = 95/161 (59%), Positives = 119/161 (73%)
 Frame = +2

Query: 2   ASVASLALLPDQGFRFTLVTSSLAIHFFKRLFEVTFIHKYSGSMPKDVAITISTSYLLSA 181
           A +AS  + P QG RFT++ S++ +H+FKR+FEV FIHKYSG M  + AI I+ SY LSA
Sbjct: 78  AGLASFWVFPHQGLRFTILQSAVTLHYFKRVFEVLFIHKYSGGMTLESAIPITLSYFLSA 137

Query: 182 ISMIYSQYLTNEFTEPLIDLKYAGMAIFSVGIIGNFYHHYLLSKLRKDGKKEYIIPRGGL 361
           ++M+YSQ+LT  F EP I+L Y G+ +F VGIIGNFYHHYLLSKLR  G+KEY IP+GG 
Sbjct: 138 VTMVYSQHLTKGFPEPPINLFYPGIVLFLVGIIGNFYHHYLLSKLRGKGEKEYKIPKGGF 197

Query: 362 FNLVICPHYLFEILGFIGIACISQNVYALSYTVGVIGYLTG 484
           F LVICPHY FEI  F GI  ISQ +Y+ ++ VG   YL G
Sbjct: 198 FELVICPHYFFEITVFYGIFFISQTLYSFAFAVGTTMYLVG 238


>ref|XP_002275776.1| PREDICTED: trans-2,3-enoyl-CoA reductase [Vitis vinifera]
          Length = 258

 Score =  192 bits (487), Expect(2) = 6e-48
 Identities = 94/158 (59%), Positives = 114/158 (72%)
 Frame = +2

Query: 11  ASLALLPDQGFRFTLVTSSLAIHFFKRLFEVTFIHKYSGSMPKDVAITISTSYLLSAISM 190
           AS  + P+  FR  LV S+L IHFFKR+ E+ F+HKYSG M  D  I IS SY LS  +M
Sbjct: 73  ASFWIFPENNFRSLLVASALTIHFFKRVLEILFVHKYSGGMVLDSGILISLSYTLSTATM 132

Query: 191 IYSQYLTNEFTEPLIDLKYAGMAIFSVGIIGNFYHHYLLSKLRKDGKKEYIIPRGGLFNL 370
           IY Q+L     EP IDLKY G+ +F VGI GNFYHH LLS+LR+  +K Y IPRGGLF+L
Sbjct: 133 IYIQHLVQGSMEPSIDLKYPGILLFLVGIYGNFYHHLLLSRLRRKDEKGYKIPRGGLFHL 192

Query: 371 VICPHYLFEILGFIGIACISQNVYALSYTVGVIGYLTG 484
           VICPHYLFEI+GFIG++ ISQ +YA S+T+G   YL G
Sbjct: 193 VICPHYLFEIIGFIGVSFISQTLYASSFTLGTTVYLIG 230



 Score = 24.3 bits (51), Expect(2) = 6e-48
 Identities = 9/11 (81%), Positives = 11/11 (100%)
 Frame = +1

Query: 481 RDVKALIPYIF 513
           R+VKAL+PYIF
Sbjct: 248 REVKALVPYIF 258