BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00017700
         (705 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAN69116.1| hypothetical protein VITISV_031843 [Vitis vinifera]   190   3e-66
ref|XP_002284795.1| PREDICTED: F-box protein SKIP23-like [Vitis ...   188   1e-65
ref|XP_002510618.1| ubiquitin-protein ligase, putative [Ricinus ...   174   2e-59
ref|XP_002300674.1| predicted protein [Populus trichocarpa] gi|2...   161   4e-57
gb|ADN34130.1| ubiquitin-protein ligase [Cucumis melo subsp. melo]    136   6e-44

>emb|CAN69116.1| hypothetical protein VITISV_031843 [Vitis vinifera]
          Length = 389

 Score =  190 bits (482), Expect(2) = 3e-66
 Identities = 87/145 (60%), Positives = 112/145 (77%)
 Frame = +1

Query: 4   DGSDYIVVAIINQTGDIAYCKKGDVSWKIIEDAHGYCEDVVYVDGMFVAVNKYGEIAMCD 183
           +GSD+I V I+NQTGD+A C+K D SW  +EDA  Y EDV++  G+F AVNKYG IA+CD
Sbjct: 173 NGSDFIAVVILNQTGDLALCRKSDDSWTFLEDAQSYSEDVIFHRGLFYAVNKYGSIAVCD 232

Query: 184 VNGDSPKVSFINTPHQVGGDMQYLVTCRNDLLLVTRYLELEFNGDANQLDIIYRTTEFQV 363
           V+G SP+VS I  P Q GGD+QYLV    +LLLVTRYL+L ++ + +Q  +IYRTT FQV
Sbjct: 233 VSGSSPRVSIIEMPQQFGGDLQYLVESGEELLLVTRYLDLTYDVEXDQTSLIYRTTRFQV 292

Query: 364 CRLNSKEVKWEILKSLGDKALFLGE 438
           CRL+SK  +WE++ SLGD+ALFLGE
Sbjct: 293 CRLDSKAPRWEVVSSLGDRALFLGE 317



 Score = 88.2 bits (217), Expect(2) = 3e-66
 Identities = 40/64 (62%), Positives = 48/64 (75%)
 Frame = +2

Query: 512 ALSASDFPECKGNRIYFTDDYSEWNYDGVHGDHDLGVYNLEDGSFEDLPCYSSNSNSTRS 691
           +LS+ DFP CKGN IY+TDDYSE NYDG+ G+ DLG++NLEDGS E LPC    S+    
Sbjct: 322 SLSSVDFPGCKGNCIYYTDDYSEDNYDGIGGEQDLGIFNLEDGSIEPLPC----SHFRIR 377

Query: 692 WPPP 703
           WPPP
Sbjct: 378 WPPP 381


>ref|XP_002284795.1| PREDICTED: F-box protein SKIP23-like [Vitis vinifera]
          Length = 389

 Score =  188 bits (477), Expect(2) = 1e-65
 Identities = 86/145 (59%), Positives = 111/145 (76%)
 Frame = +1

Query: 4   DGSDYIVVAIINQTGDIAYCKKGDVSWKIIEDAHGYCEDVVYVDGMFVAVNKYGEIAMCD 183
           +GSD+I V I+NQTGD+A C+K D SW  +EDA  Y EDV++  G+F AVNKYG IA+CD
Sbjct: 173 NGSDFIAVVILNQTGDLALCRKSDDSWTFLEDAQSYAEDVIFHRGLFYAVNKYGSIAVCD 232

Query: 184 VNGDSPKVSFINTPHQVGGDMQYLVTCRNDLLLVTRYLELEFNGDANQLDIIYRTTEFQV 363
           V+G SP+VS I  P Q GGD+QYLV    +LLLVTRYL+L ++ + +Q  +IYRTT FQV
Sbjct: 233 VSGSSPRVSIIEMPQQFGGDLQYLVESGEELLLVTRYLDLTYDVEPDQTSLIYRTTRFQV 292

Query: 364 CRLNSKEVKWEILKSLGDKALFLGE 438
           CRL+ K  +WE++ SLGD+ALFLGE
Sbjct: 293 CRLDLKAPRWEVVSSLGDRALFLGE 317



 Score = 88.2 bits (217), Expect(2) = 1e-65
 Identities = 40/64 (62%), Positives = 48/64 (75%)
 Frame = +2

Query: 512 ALSASDFPECKGNRIYFTDDYSEWNYDGVHGDHDLGVYNLEDGSFEDLPCYSSNSNSTRS 691
           +LS+ DFP CKGN IY+TDDYSE NYDG+ G+ DLG++NLEDGS E LPC    S+    
Sbjct: 322 SLSSVDFPGCKGNCIYYTDDYSEDNYDGIGGEQDLGIFNLEDGSIEPLPC----SHFRIR 377

Query: 692 WPPP 703
           WPPP
Sbjct: 378 WPPP 381


>ref|XP_002510618.1| ubiquitin-protein ligase, putative [Ricinus communis]
           gi|223551319|gb|EEF52805.1| ubiquitin-protein ligase,
           putative [Ricinus communis]
          Length = 398

 Score =  174 bits (440), Expect(2) = 2e-59
 Identities = 78/145 (53%), Positives = 112/145 (77%), Gaps = 2/145 (1%)
 Frame = +1

Query: 10  SDYIVVAIINQTGDIAYCKKGDVSWKIIEDAHGYCEDVVYVDGMFVAVNKYGEIAMCDVN 189
           ++++V+AI+NQT D+AYC+ GD SW ++E+A  +CEDV++V+GMF AVNK G+I +CD++
Sbjct: 178 NNFVVIAILNQTDDLAYCRNGDKSWSLVENARSFCEDVIFVNGMFYAVNKAGQIVICDIS 237

Query: 190 GDSPKVSFINTPHQVGGDMQYLVTCRNDLLLVTRYLELEFNGDANQLD--IIYRTTEFQV 363
            DSP+VSF+ TP Q GGDMQYLV   ++LLLVTRYL+LE+  D   ++  +IYRT  F+V
Sbjct: 238 SDSPRVSFMETPRQAGGDMQYLVNSGDELLLVTRYLDLEYEFDHPDMEPHLIYRTIRFEV 297

Query: 364 CRLNSKEVKWEILKSLGDKALFLGE 438
            RL+    +W  + +LGDKALF+GE
Sbjct: 298 FRLDWNGPQWLRMSTLGDKALFIGE 322



 Score = 82.0 bits (201), Expect(2) = 2e-59
 Identities = 36/64 (56%), Positives = 43/64 (67%)
 Frame = +2

Query: 512 ALSASDFPECKGNRIYFTDDYSEWNYDGVHGDHDLGVYNLEDGSFEDLPCYSSNSNSTRS 691
           +LSA+DF  C GN IY+TDDY + NYDG  GDHDLG++ L D + E LPCY  N  S   
Sbjct: 327 SLSATDFSGCMGNCIYYTDDYLDANYDGHFGDHDLGIFKLCDETIEPLPCYPRNFTSPLQ 386

Query: 692 WPPP 703
           W PP
Sbjct: 387 WSPP 390


>ref|XP_002300674.1| predicted protein [Populus trichocarpa] gi|222842400|gb|EEE79947.1|
           predicted protein [Populus trichocarpa]
          Length = 393

 Score =  161 bits (408), Expect(2) = 4e-57
 Identities = 76/144 (52%), Positives = 103/144 (71%)
 Frame = +1

Query: 7   GSDYIVVAIINQTGDIAYCKKGDVSWKIIEDAHGYCEDVVYVDGMFVAVNKYGEIAMCDV 186
           G  ++ +AI+  TGD+AYCK GD  W I+E++  + EDV+Y +G+F AVNK G+I +CDV
Sbjct: 178 GDFFVAMAILCSTGDLAYCKNGDECWNIVENSRSFSEDVIYFNGLFYAVNKAGQIVVCDV 237

Query: 187 NGDSPKVSFINTPHQVGGDMQYLVTCRNDLLLVTRYLELEFNGDANQLDIIYRTTEFQVC 366
           NG+SPKVSFI TP Q+GGDMQYLV+  + LLLVTRYL+L+   D      IY+T  F+V 
Sbjct: 238 NGNSPKVSFIETPRQIGGDMQYLVSSGDGLLLVTRYLDLDVEFDI----FIYKTARFEVF 293

Query: 367 RLNSKEVKWEILKSLGDKALFLGE 438
           +L+    +WE + SLGD  LF+GE
Sbjct: 294 KLDLNGPRWERVTSLGDMMLFIGE 317



 Score = 86.3 bits (212), Expect(2) = 4e-57
 Identities = 39/64 (60%), Positives = 44/64 (68%)
 Frame = +2

Query: 512 ALSASDFPECKGNRIYFTDDYSEWNYDGVHGDHDLGVYNLEDGSFEDLPCYSSNSNSTRS 691
           ALSAS    C G+ IY+TDDYS  NYDG  G+HDLG++ L DGS E LPCY  NS S   
Sbjct: 322 ALSASGLSGCMGDCIYYTDDYSANNYDGHIGEHDLGIFKLSDGSIEPLPCYPRNSYSRLQ 381

Query: 692 WPPP 703
           WPPP
Sbjct: 382 WPPP 385


>gb|ADN34130.1| ubiquitin-protein ligase [Cucumis melo subsp. melo]
          Length = 392

 Score =  136 bits (342), Expect(2) = 6e-44
 Identities = 61/143 (42%), Positives = 99/143 (69%), Gaps = 1/143 (0%)
 Frame = +1

Query: 10  SDYIVVAIINQTGDIAYCKKGDVSWKIIEDAHGYCEDVVYVDGMFVAVNKYGEIAMCDVN 189
           +D++ VAI+N +GD+A+C+ G  SW  ++DA   CEDV+Y DG+F AV+KYG +++ D+ 
Sbjct: 173 NDFLAVAILNHSGDLAFCRSGGGSWTFVDDAPSDCEDVIYTDGVFYAVDKYGVVSLMDLR 232

Query: 190 GDSPKVSFINTPHQVGGDMQYLVTCRNDLLLVTRYLELEFNG-DANQLDIIYRTTEFQVC 366
           G   +VS + T  Q+ GD+QYLV    +LLLV+RYL++  +G D   + ++YRT  F+V 
Sbjct: 233 GSRSQVSLVATERQLAGDIQYLVRLGQELLLVSRYLDIVNDGMDDELISVMYRTVRFEVF 292

Query: 367 RLNSKEVKWEILKSLGDKALFLG 435
           R+  +  +WE +++L + ALF+G
Sbjct: 293 RMEWEGPRWEKVENLNEMALFVG 315



 Score = 67.8 bits (164), Expect(2) = 6e-44
 Identities = 33/64 (51%), Positives = 42/64 (65%)
 Frame = +2

Query: 512 ALSASDFPECKGNRIYFTDDYSEWNYDGVHGDHDLGVYNLEDGSFEDLPCYSSNSNSTRS 691
           A SA+DF E  GN IY+TDDYS+ +Y G   + D+G++ L D SFE LP YS  S+S R 
Sbjct: 321 AFSAADFGEISGNCIYYTDDYSDSDYQGQGEEPDMGIFRLCDESFEPLPYYSGGSHSRRR 380

Query: 692 WPPP 703
             PP
Sbjct: 381 LLPP 384