BLASTX nr result
ID: Bupleurum21_contig00017647
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00017647
(1998 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002300226.1| predicted protein [Populus trichocarpa] gi|2... 220 3e-72
emb|CBI32383.3| unnamed protein product [Vitis vinifera] 165 2e-56
ref|XP_002282435.1| PREDICTED: filament-like plant protein 7-lik... 147 2e-43
ref|XP_002307274.1| predicted protein [Populus trichocarpa] gi|2... 129 5e-36
ref|XP_002313860.1| predicted protein [Populus trichocarpa] gi|2... 92 2e-34
>ref|XP_002300226.1| predicted protein [Populus trichocarpa] gi|222847484|gb|EEE85031.1|
predicted protein [Populus trichocarpa]
Length = 893
Score = 220 bits (560), Expect(2) = 3e-72
Identities = 147/405 (36%), Positives = 209/405 (51%), Gaps = 15/405 (3%)
Frame = +2
Query: 68 NSSESYGQNSSFLIGRLTIVEEENKILRELLIKKDDEIQSSMTLFSQTASKLSQVEAQLK 247
N E+ +N FLI +L EEENK LRE++ +K+ E+QSS +FS+TAS+LSQVEAQL
Sbjct: 312 NFPETPVKNVDFLIDQLLGKEEENKALREMMSRKNAELQSSRIMFSRTASRLSQVEAQLV 371
Query: 248 YLLKDPKLVKMVMCXXXXXXXXXXXXXLAYDDEQVXXXXXXXXXXXXXXXXNASECKLIG 427
L D + +++ + + SECK I
Sbjct: 372 ELSGDQRSLELAKHSPSSREIHSPTAG-SDTGSLANALIAELEHFRDRKLKSPSECKDIE 430
Query: 428 VSDISLMDDFVEMEKLAIVAVNSPPGSSHVSSDNTLSNRFTSNSREHDLETNGKELVPVE 607
V D+SLMDDFVEMEKLAIV+ +P S+ +S + +H + K+ + +
Sbjct: 431 VLDMSLMDDFVEMEKLAIVSTQTP--SAGGNSSFSAGKELVPIELDHSGYRDKKQEIHSK 488
Query: 608 PLVESSDVCWLKDVLKVILNQMRISNKNLDELLEDIKVAFSNSNHTSLKNSEQSGL---- 775
WL+ VL I Q RIS ++L ELLEDIK+A NH + + + L
Sbjct: 489 QDSTDKSFDWLQVVLNAIFTQQRISKRSLTELLEDIKIALGYINHPNASEPDAAALSRHP 548
Query: 776 --LPVSGYITWKTPTSSPLMKCRKKMSDIGILVEDRECSKNELNTVKDQDED-------- 925
+ GYITWK+P S + + S I +++ ++ N Q+E+
Sbjct: 549 LECDIGGYITWKSPNESSIANSLNETSSIDTPMKETSKQHDQSNLSNLQEENGRLENELD 608
Query: 926 -LQLMLKEANDKNDELINQLREKEKRIGSLQTXXXXXXXXXXXXXXXXXNQKLINEDLNM 1102
++ L+ A DK + L+ +LRE E+R+ LQ NQK INEDL+
Sbjct: 609 NMEARLQSATDKTETLMVKLRESEQRVERLQAEVEILKESKGMVEDQIENQKSINEDLDT 668
Query: 1103 QLTVSNLELNKLYEKLSYLEVELEERSRCCEEFEATCLELGLQLE 1237
QLTV+ +LN++++K S LEVELE+RS CCEE EATCLEL LQLE
Sbjct: 669 QLTVTKAKLNEVFQKFSSLEVELEDRSNCCEELEATCLELQLQLE 713
Score = 80.5 bits (197), Expect(2) = 3e-72
Identities = 53/140 (37%), Positives = 76/140 (54%), Gaps = 4/140 (2%)
Frame = +1
Query: 1342 MDQDKKLLQTDWEMNAASAKLEECQETIFNLGKQLKALSSRKEAS--DSVKVVSNPNTIE 1515
++++ K Q WE+ AAS KL ECQETI NLGKQLKAL+S +EA+ D V + T
Sbjct: 724 INKEGKHPQDGWEIKAASVKLAECQETILNLGKQLKALASPREAALFDKVFTTTGATTTA 783
Query: 1516 SKSK-LNNRSSLRD-ILDERNAEPINLESPXXXXXXXXXXXXXXXNNYNSQNLMISPKDH 1689
+ + LN R SLRD +L E ++ I L SP N S N ++ +
Sbjct: 784 TNIRNLNKRFSLRDQMLAEDRSKEIILRSPTEDAQKSSLDHSDNGNGLISPNALVCAPEA 843
Query: 1690 FLGRKHEALTPLVGTMAIIP 1749
++G +H+A V +AI+P
Sbjct: 844 YIGPEHKAGDAAVEALAIVP 863
>emb|CBI32383.3| unnamed protein product [Vitis vinifera]
Length = 632
Score = 165 bits (417), Expect(2) = 2e-56
Identities = 126/384 (32%), Positives = 172/384 (44%), Gaps = 9/384 (2%)
Frame = +2
Query: 113 RLTIVEEENKILRELLIKKDDEIQSSMTLFSQTASKLSQVEAQLKYLLKDPKLVKMVMCX 292
RL VEEENK L+E+L KK++E+ S L ++T S+ Q EAQL K K + +V
Sbjct: 254 RLCEVEEENKTLKEILAKKNNELHSPRLLCARTPSRFGQPEAQLGESPKSQKTMDLV--- 310
Query: 293 XXXXXXXXXXXXLAYDDEQVXXXXXXXXXXXXXXXXNASECKLIGVSDISLMDDFVEMEK 472
S +SLMDDFVEMEK
Sbjct: 311 ----------------------------------------------SYMSLMDDFVEMEK 324
Query: 473 LAIVAVNSPPGSSHVSSDNTLSNRFTSNSREHDLETNGKELVPVEPLVESSDVCWLKDVL 652
LAIV+ ++ SH S SR+ WL+DVL
Sbjct: 325 LAIVSADTHFQGSHDGS--------IGKSRD-----------------------WLQDVL 353
Query: 653 KVILNQMRISNKNLDELLEDIKVAFSNSNHTSLKNSEQSGLL---------PVSGYITWK 805
KV+L Q +S ++L ELL+DIK+A N S+ ++++ P+SGYITWK
Sbjct: 354 KVMLEQNCVSKRSLHELLDDIKIALGFVNDPSVVEADKAASSRHLGEPDSQPISGYITWK 413
Query: 806 TPTSSPLMKCRKKMSDIGILVEDRECSKNELNTVKDQDEDLQLMLKEANDKNDELINQLR 985
+K DL+ L+ A D + L+NQL
Sbjct: 414 DEIKRHFESAKK---------------------------DLEAKLQSATDNSQALMNQLE 446
Query: 986 EKEKRIGSLQTXXXXXXXXXXXXXXXXXNQKLINEDLNMQLTVSNLELNKLYEKLSYLEV 1165
+ E+ IGSL+T NQKLINE+LN QLTV+ ++N++ +K S LEV
Sbjct: 447 KSEQSIGSLRTELETLKDSKGLIEDQIENQKLINEELNTQLTVAKAKINEVLQKFSALEV 506
Query: 1166 ELEERSRCCEEFEATCLELGLQLE 1237
E E++S C+E EATCLEL LQLE
Sbjct: 507 EFEDKSNSCQELEATCLELQLQLE 530
Score = 82.8 bits (203), Expect(2) = 2e-56
Identities = 46/94 (48%), Positives = 64/94 (68%), Gaps = 1/94 (1%)
Frame = +1
Query: 1321 KNLPKKEMDQDKKLLQTDWEMNAASAKLEECQETIFNLGKQLKALSSRKEASDSVKVVSN 1500
K + + + DQ+ K LQT WE+ AAS KL ECQETI NLGKQLKAL+S ++ + KV S
Sbjct: 535 KEVSEADQDQEGKQLQTGWEITAASVKLAECQETILNLGKQLKALASPRDRAIFDKVYST 594
Query: 1501 PNTIESKSKLNNRSSLRD-ILDERNAEPINLESP 1599
+T + KL++RSSLRD +L + +A+ +SP
Sbjct: 595 TSTATNDKKLSHRSSLRDRMLADDDADTEVFKSP 628
>ref|XP_002282435.1| PREDICTED: filament-like plant protein 7-like [Vitis vinifera]
Length = 1098
Score = 104 bits (260), Expect(2) = 2e-43
Identities = 55/125 (44%), Positives = 77/125 (61%)
Frame = +2
Query: 863 LVEDRECSKNELNTVKDQDEDLQLMLKEANDKNDELINQLREKEKRIGSLQTXXXXXXXX 1042
L E+ K+EL ++ +DL+ L+ A D + L+NQL + E+ IGSL+T
Sbjct: 790 LQEENRGLKDELRKIESAKKDLEAKLQSATDNSQALMNQLEKSEQSIGSLRTELETLKDS 849
Query: 1043 XXXXXXXXXNQKLINEDLNMQLTVSNLELNKLYEKLSYLEVELEERSRCCEEFEATCLEL 1222
NQKLINE+LN QLTV+ ++N++ +K S LEVE E++S C+E EATCLEL
Sbjct: 850 KGLIEDQIENQKLINEELNTQLTVAKAKINEVLQKFSALEVEFEDKSNSCQELEATCLEL 909
Query: 1223 GLQLE 1237
LQLE
Sbjct: 910 QLQLE 914
Score = 99.8 bits (247), Expect(2) = 2e-43
Identities = 58/149 (38%), Positives = 88/149 (59%), Gaps = 6/149 (4%)
Frame = +1
Query: 1321 KNLPKKEMDQDKKLLQTDWEMNAASAKLEECQETIFNLGKQLKALSSRKEASDSVKVVSN 1500
K + + + DQ+ K LQT WE+ AAS KL ECQETI NLGKQLKAL+S ++ + KV S
Sbjct: 919 KEVSEADQDQEGKQLQTGWEITAASVKLAECQETILNLGKQLKALASPRDRAIFDKVYST 978
Query: 1501 PNTIESKSKLNNRSSLRD-ILDERNAEPINLESP-----XXXXXXXXXXXXXXXNNYNSQ 1662
+T + KL++RSSLRD +L + +A+ +SP N++++
Sbjct: 979 TSTATNDKKLSHRSSLRDRMLADDDADTEVFKSPKIKEIISTAHIPSTLGSNNSNSFDAP 1038
Query: 1663 NLMISPKDHFLGRKHEALTPLVGTMAIIP 1749
++ + D + KH A+TP VG++AI+P
Sbjct: 1039 DIHVEAPDAYHDSKHRAVTPAVGSLAIVP 1067
Score = 147 bits (372), Expect = 8e-33
Identities = 121/347 (34%), Positives = 167/347 (48%), Gaps = 36/347 (10%)
Frame = +2
Query: 5 TETMRRSMSNTTGQSTART--VENSSESYGQNSSFLIGRLTIVEEENKILRELLIKKDDE 178
TE R+ ++ TG AR VE SSE + SFLI RL VEEENK L+E+L KK++E
Sbjct: 293 TEMRRKKLNPMTGGLIARDGLVEKSSEIPSKKMSFLIERLCEVEEENKTLKEILAKKNNE 352
Query: 179 IQSSMTLFSQTASKLSQVEAQLKYLLKDPKLVKMVMCXXXXXXXXXXXXXLAYDDEQVXX 358
+ S L ++T S+ Q EAQL K K + +V C D+ +
Sbjct: 353 LHSPRLLCARTPSRFGQPEAQLGESPKSQKTMDLVSCSPISNGHSLPSGFDIGSDDGISS 412
Query: 359 XXXXXXXXXXXXXX-------NASECKLIGVSDISLMDDFVEMEKLAIVAVNSPPGSSHV 517
N SECK I VSD+SLMDDFVEMEKLAIV+ ++ SHV
Sbjct: 413 SGSWANALISELEQFRHAKPKNPSECKTI-VSDMSLMDDFVEMEKLAIVSADTHFQGSHV 471
Query: 518 SSD--NTLSNRFTSNSREHDLETNGKELVPVEPLVESSDVC----------------WLK 643
S+ N +N S ++ GKELVPV SS WL+
Sbjct: 472 PSNTRNASANTLEKESGGFLSDSTGKELVPVAQDYSSSTDTKWETQSKDGSIGKSRDWLQ 531
Query: 644 DVLKVILNQMRISNKNLDELLEDIKVAFSNSNHTSLKNSEQSG---------LLPVSGYI 796
DVLKV+L Q +S ++L ELL+DIK+A N S+ ++++ P+SGYI
Sbjct: 532 DVLKVMLEQNCVSKRSLHELLDDIKIALGFVNDPSVVEADKAASSRHLGEPDSQPISGYI 591
Query: 797 TWKTPTSSPLMKCRKKMSDIGILVEDRECSKNELNTVKDQDEDLQLM 937
TWK+ P+ K S I VE +N+ + K + ++L+
Sbjct: 592 TWKS-MEFPMAGSLHKGSVIDTSVEGASHQQNQSDLSKSICKIIELI 637
>ref|XP_002307274.1| predicted protein [Populus trichocarpa] gi|222856723|gb|EEE94270.1|
predicted protein [Populus trichocarpa]
Length = 947
Score = 108 bits (270), Expect(2) = 5e-36
Identities = 57/134 (42%), Positives = 81/134 (60%)
Frame = +2
Query: 836 RKKMSDIGILVEDRECSKNELNTVKDQDEDLQLMLKEANDKNDELINQLREKEKRIGSLQ 1015
+K +SD + ++ + EL + DL+ L+ A+DK++ L+NQL+E EK I SLQ
Sbjct: 624 KKDVSDQSTIRDENRKIREELTNIDSAKRDLEARLQLASDKSEPLMNQLKESEKTIESLQ 683
Query: 1016 TXXXXXXXXXXXXXXXXXNQKLINEDLNMQLTVSNLELNKLYEKLSYLEVELEERSRCCE 1195
T N KL+ ED++ +LT + +ELNK ++KLS LE+ELE R CCE
Sbjct: 684 TDLETLRGSKAMFESQIENHKLMKEDVDTELTEAKVELNKAHQKLSTLEMELENRKSCCE 743
Query: 1196 EFEATCLELGLQLE 1237
E EATCLEL +QLE
Sbjct: 744 ELEATCLELQIQLE 757
Score = 71.2 bits (173), Expect(2) = 5e-36
Identities = 38/65 (58%), Positives = 47/65 (72%), Gaps = 3/65 (4%)
Frame = +1
Query: 1327 LPKKEMDQDKKLLQTDWEMNAASAKLEECQETIFNLGKQLKALSSRKEASDSVKVVS--- 1497
+P E+ QD+ L+TDWE+ AAS KL ECQETI NLGKQLKAL+S EA+ KV+S
Sbjct: 764 IPNSEVHQDESQLRTDWEITAASEKLAECQETILNLGKQLKALASPSEAALFDKVISTST 823
Query: 1498 NPNTI 1512
+ NTI
Sbjct: 824 DTNTI 828
Score = 129 bits (325), Expect = 2e-27
Identities = 100/299 (33%), Positives = 145/299 (48%), Gaps = 30/299 (10%)
Frame = +2
Query: 17 RRSMSNTTGQSTARTVENSSESYGQNSSFLIGRLTIVEEENKILRELLIKKDDEIQSSMT 196
RRS + G V NS+ES + +FL +L +EEENK L+E L KK +E+Q S T
Sbjct: 179 RRSNCSPIGLVVDSAVGNSAESPSKKINFLTEQLCAMEEENKTLKEALDKKTNELQVSRT 238
Query: 197 LFSQTASKLSQVEAQLKYLLKDPKLVKMVMCXXXXXXXXXXXXXLAYDDEQVXXXXXXXX 376
++++TASKLSQVE+ L K ++ D++V
Sbjct: 239 MYARTASKLSQVESLFDELPKGQITLERSRSVRMPQELSLASMSEIGSDDKVSSAESWAS 298
Query: 377 XXXXXXXX-------NASECKLIGVSDISLMDDFVEMEKLAIVAVNSPPGSSHVSSDNTL 535
+ + IGVSDISLMDDF EME+LAIV+V+ S H SSDN
Sbjct: 299 ALISEMEHFKQGKQKGSPTNRTIGVSDISLMDDFAEMERLAIVSVDKQLESPHASSDN-- 356
Query: 536 SNRFTSNSREHDLETNGKELVPV--------EPLVESSDVC--WLKDVLKVILNQMRISN 685
+ G+E++PV +++S D WL D+LKV+L Q R++
Sbjct: 357 ------------VNAIGQEIIPVSESRSGVSNQVIKSKDKASGWLHDILKVVLEQNRVTQ 404
Query: 686 KNLDELLEDIKVAFSNSNH--------TSLKNSEQSGLLP--VSGYITWK---TPTSSP 823
+ E+LED+++A +N NH T ++ +GL V GYI+WK + T SP
Sbjct: 405 RKPCEILEDVRIALANINHASPAEYVDTRQSSTHSNGLNSPHVGGYISWKPMYSVTDSP 463
>ref|XP_002313860.1| predicted protein [Populus trichocarpa] gi|222850268|gb|EEE87815.1|
predicted protein [Populus trichocarpa]
Length = 284
Score = 92.4 bits (228), Expect(2) = 2e-34
Identities = 48/104 (46%), Positives = 64/104 (61%)
Frame = +2
Query: 926 LQLMLKEANDKNDELINQLREKEKRIGSLQTXXXXXXXXXXXXXXXXXNQKLINEDLNMQ 1105
++ L A DK++ L+ +LRE E+ + LQ NQK INEDL+ Q
Sbjct: 1 MEARLHSATDKSEALMMKLRESEQSVERLQAEVEILKESKGMIEDQIENQKSINEDLDTQ 60
Query: 1106 LTVSNLELNKLYEKLSYLEVELEERSRCCEEFEATCLELGLQLE 1237
LTV+ +LN+++ K S LEVE E++S CCEE EATCLEL LQLE
Sbjct: 61 LTVTKAKLNEVFHKFSSLEVEFEDKSNCCEELEATCLELQLQLE 104
Score = 82.4 bits (202), Expect(2) = 2e-34
Identities = 51/140 (36%), Positives = 75/140 (53%), Gaps = 4/140 (2%)
Frame = +1
Query: 1342 MDQDKKLLQTDWEMNAASAKLEECQETIFNLGKQLKALSSRKEASDSVKVVSNPNTIESK 1521
++++ K Q WE+ AAS KL ECQETI NLGKQLKAL+S +EA+ KV + +
Sbjct: 115 INEEGKQPQDGWEIKAASVKLAECQETILNLGKQLKALASPREAALFDKVFTTTGATAAA 174
Query: 1522 S---KLNNRSSLRD-ILDERNAEPINLESPXXXXXXXXXXXXXXXNNYNSQNLMISPKDH 1689
+ +N R SLRD ++ E ++ I L SP N S N ++ +
Sbjct: 175 TNIKNMNRRFSLRDQMIAEDRSKAIILRSPTEDAQKSSLNHTDNGNELISPNALVCASEA 234
Query: 1690 FLGRKHEALTPLVGTMAIIP 1749
+ G KH++ VG +AI+P
Sbjct: 235 YFGPKHKSGNAAVGALAIVP 254