BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00017502
         (1197 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CBI24343.3| unnamed protein product [Vitis vinifera]              525   e-147
ref|XP_002272310.1| PREDICTED: protein kinase 2B, chloroplastic-...   525   e-147
ref|XP_003543286.1| PREDICTED: protein kinase 2A, chloroplastic-...   480   e-133
ref|XP_004149666.1| PREDICTED: protein kinase 2B, chloroplastic-...   476   e-132
ref|XP_002313478.1| predicted protein [Populus trichocarpa] gi|2...   473   e-131

>emb|CBI24343.3| unnamed protein product [Vitis vinifera]
          Length = 422

 Score =  525 bits (1352), Expect = e-147
 Identities = 263/381 (69%), Positives = 315/381 (82%), Gaps = 4/381 (1%)
 Frame = +2

Query: 26   MGTCLGKSAKCAHASSNQFN---ETTSPTSDKQQNSFSPQKKSVSLSFNKTVGASEGEIT 196
            MG C  + AKCAHASS  F+   + T P S   ++S S  + +   + NK + +S+ +I+
Sbjct: 1    MGNCCRRPAKCAHASSTHFSVQSDRTKPPSKANKDSTSSSQHTPFGTLNKILVSSKSDIS 60

Query: 197  VSNNLRAFSYHDLKNAAKNFRSDSLLGEGGFGWVFKAWLDENSFGPSKPGAGISVAIKKL 376
             SN+++ FS++DLKNA+KNFRS+SLLGEGGFG VFK WLDEN+  P+KPG G+ VAIKKL
Sbjct: 61   ASNSVKDFSFNDLKNASKNFRSESLLGEGGFGCVFKGWLDENTLAPTKPGTGMVVAIKKL 120

Query: 377  KTGSCQGHREWLTEVNYLGQLHHQNLVKLIGYCFEADNRLLVYEFMSKGSLENHLFRKGV 556
            KT S QGH+EWL EVNYLGQLHH+NLVKLIGYC E++NRLLVYEFMSKGSLENHLF+KGV
Sbjct: 121  KTESFQGHKEWLAEVNYLGQLHHENLVKLIGYCSESENRLLVYEFMSKGSLENHLFKKGV 180

Query: 557  EPMPWPTRIRIATDVARGLSFLHNLDANVIYRDLKASNILLDSDFNAKLSDFGLARDGPT 736
            +P+ W TR+ IA DVA+G+SFLH+LDANVIYRDLKASNILLDSDF AKLSDFGLARDGPT
Sbjct: 181  QPITWATRMSIAIDVAQGISFLHSLDANVIYRDLKASNILLDSDFKAKLSDFGLARDGPT 240

Query: 737  GDKTHVSTRVVGTKGYAAPEYVATGRLTPKSDVYSFGVVLLELLSGRRALGDERAGGVDE 916
            GD THVSTRVVGT+GYAAPEYVATG LTPKSDVYSFGVVLLELLSGRRA+ DE+AGGV E
Sbjct: 241  GDNTHVSTRVVGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGRRAMDDEKAGGV-E 299

Query: 917  ETLVDWAKPLLGE-KRLLRIMDSRMGGQYSKKEAQAVASLAYLCLNFDPKIRPSMLEVVG 1093
            ETLVDWAKP L + +R+LRIMD+R+GGQYSKK AQA ASLA  CL+ DPK RP M +V+ 
Sbjct: 300  ETLVDWAKPFLSDNRRVLRIMDTRLGGQYSKKGAQAAASLALQCLHTDPKNRPLMTDVLA 359

Query: 1094 KLEQIPLAKNVSRTSQVKQEH 1156
             LE++P +K++ RT   + E+
Sbjct: 360  ALERLPTSKDIPRTPSPRVEY 380


>ref|XP_002272310.1| PREDICTED: protein kinase 2B, chloroplastic-like [Vitis vinifera]
          Length = 403

 Score =  525 bits (1352), Expect = e-147
 Identities = 263/381 (69%), Positives = 315/381 (82%), Gaps = 4/381 (1%)
 Frame = +2

Query: 26   MGTCLGKSAKCAHASSNQFN---ETTSPTSDKQQNSFSPQKKSVSLSFNKTVGASEGEIT 196
            MG C  + AKCAHASS  F+   + T P S   ++S S  + +   + NK + +S+ +I+
Sbjct: 1    MGNCCRRPAKCAHASSTHFSVQSDRTKPPSKANKDSTSSSQHTPFGTLNKILVSSKSDIS 60

Query: 197  VSNNLRAFSYHDLKNAAKNFRSDSLLGEGGFGWVFKAWLDENSFGPSKPGAGISVAIKKL 376
             SN+++ FS++DLKNA+KNFRS+SLLGEGGFG VFK WLDEN+  P+KPG G+ VAIKKL
Sbjct: 61   ASNSVKDFSFNDLKNASKNFRSESLLGEGGFGCVFKGWLDENTLAPTKPGTGMVVAIKKL 120

Query: 377  KTGSCQGHREWLTEVNYLGQLHHQNLVKLIGYCFEADNRLLVYEFMSKGSLENHLFRKGV 556
            KT S QGH+EWL EVNYLGQLHH+NLVKLIGYC E++NRLLVYEFMSKGSLENHLF+KGV
Sbjct: 121  KTESFQGHKEWLAEVNYLGQLHHENLVKLIGYCSESENRLLVYEFMSKGSLENHLFKKGV 180

Query: 557  EPMPWPTRIRIATDVARGLSFLHNLDANVIYRDLKASNILLDSDFNAKLSDFGLARDGPT 736
            +P+ W TR+ IA DVA+G+SFLH+LDANVIYRDLKASNILLDSDF AKLSDFGLARDGPT
Sbjct: 181  QPITWATRMSIAIDVAQGISFLHSLDANVIYRDLKASNILLDSDFKAKLSDFGLARDGPT 240

Query: 737  GDKTHVSTRVVGTKGYAAPEYVATGRLTPKSDVYSFGVVLLELLSGRRALGDERAGGVDE 916
            GD THVSTRVVGT+GYAAPEYVATG LTPKSDVYSFGVVLLELLSGRRA+ DE+AGGV E
Sbjct: 241  GDNTHVSTRVVGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSGRRAMDDEKAGGV-E 299

Query: 917  ETLVDWAKPLLGE-KRLLRIMDSRMGGQYSKKEAQAVASLAYLCLNFDPKIRPSMLEVVG 1093
            ETLVDWAKP L + +R+LRIMD+R+GGQYSKK AQA ASLA  CL+ DPK RP M +V+ 
Sbjct: 300  ETLVDWAKPFLSDNRRVLRIMDTRLGGQYSKKGAQAAASLALQCLHTDPKNRPLMTDVLA 359

Query: 1094 KLEQIPLAKNVSRTSQVKQEH 1156
             LE++P +K++ RT   + E+
Sbjct: 360  ALERLPTSKDIPRTPSPRVEY 380


>ref|XP_003543286.1| PREDICTED: protein kinase 2A, chloroplastic-like [Glycine max]
          Length = 382

 Score =  480 bits (1235), Expect = e-133
 Identities = 243/371 (65%), Positives = 288/371 (77%), Gaps = 1/371 (0%)
 Frame = +2

Query: 26   MGTCLGKSAKCAHASSNQFNETTSPTSDKQQNSFSPQKKSVSLSFNKTVGASEGEITVSN 205
            MG    K    AH SS+ F+ +  P S  +Q S S +++S   +    V  S     +S+
Sbjct: 1    MGNACAKGKPVAHVSSSNFSGSKKPASKPKQYSNSSEQRSAPTTSELNVPKS-----ISS 55

Query: 206  NLRAFSYHDLKNAAKNFRSDSLLGEGGFGWVFKAWLDENSFGPSKPGAGISVAIKKLKTG 385
            NL++FS +DLK A KNFR ++L+GEGGFG VFK W+DEN++GP+KPG GI VAIK LK  
Sbjct: 56   NLKSFSLNDLKEATKNFRRENLIGEGGFGRVFKGWIDENTYGPTKPGTGIVVAIKNLKPE 115

Query: 386  SCQGHREWLTEVNYLGQLHHQNLVKLIGYCFEADNRLLVYEFMSKGSLENHLFRKGVEPM 565
            S QGH+EWL EVNYLG L H+NLVKLIGYC E  NRLLVYEFM KGSLENHLFRKGV+PM
Sbjct: 116  SFQGHKEWLQEVNYLGMLQHENLVKLIGYCLEGKNRLLVYEFMQKGSLENHLFRKGVQPM 175

Query: 566  PWPTRIRIATDVARGLSFLHNLDANVIYRDLKASNILLDSDFNAKLSDFGLARDGPTGDK 745
             W TR+ IA  VARGL+FLH+LD NVI+RDLKASNILLDSDFNAKLSDFGLARDGPTGD 
Sbjct: 176  AWVTRVNIAIGVARGLTFLHSLDQNVIFRDLKASNILLDSDFNAKLSDFGLARDGPTGDN 235

Query: 746  THVSTRVVGTKGYAAPEYVATGRLTPKSDVYSFGVVLLELLSGRRALGDERAGGVDEETL 925
            THVSTRV+GT+GYAAPEYVATG LTP+SDVYSFGVVLLELL+GRRA+ D+   G  EETL
Sbjct: 236  THVSTRVIGTQGYAAPEYVATGHLTPRSDVYSFGVVLLELLTGRRAVEDD-GPGFSEETL 294

Query: 926  VDWAKPLLGE-KRLLRIMDSRMGGQYSKKEAQAVASLAYLCLNFDPKIRPSMLEVVGKLE 1102
            VDWAKP L + +R+LRIMD+R+GGQYSKK AQA A+LA  CLN DPK RP M+EV+  LE
Sbjct: 295  VDWAKPFLNDNRRVLRIMDTRLGGQYSKKGAQAAAALALQCLNTDPKFRPPMVEVLAALE 354

Query: 1103 QIPLAKNVSRT 1135
             +  + + +RT
Sbjct: 355  ALNSSNSFTRT 365


>ref|XP_004149666.1| PREDICTED: protein kinase 2B, chloroplastic-like [Cucumis sativus]
            gi|449515432|ref|XP_004164753.1| PREDICTED: protein
            kinase 2B, chloroplastic-like [Cucumis sativus]
          Length = 403

 Score =  476 bits (1226), Expect = e-132
 Identities = 248/388 (63%), Positives = 299/388 (77%), Gaps = 18/388 (4%)
 Frame = +2

Query: 26   MGTCLGKSAKCAHASSNQFNE-----------------TTSPTSDKQQNSFSPQKKSVSL 154
            MG C  K AK AHASS+  +                  T +  S     S +   K+V+ 
Sbjct: 1    MGICCAKPAKLAHASSSIISGELSLQLFRFFVLLSAIGTDNKNSKSNVESINCNSKTVNF 60

Query: 155  SFNKTVGASEGEITVSNNLRAFSYHDLKNAAKNFRSDSLLGEGGFGWVFKAWLDENSFGP 334
            + +  V   + +++ ++ L++FS+ DLKNA KNFRS+SLLGEGGFG VFK W+DE+S+ P
Sbjct: 61   TSSAPVIKPKFDVSGASALKSFSFIDLKNATKNFRSESLLGEGGFGCVFKGWIDEHSYLP 120

Query: 335  SKPGAGISVAIKKLKTGSCQGHREWLTEVNYLGQLHHQNLVKLIGYCFEADNRLLVYEFM 514
            +KPG GI VA+KKLK  S QG++EWL EVNYLGQL H+NLV+LIGYC E+DNRLLVYE+M
Sbjct: 121  TKPGTGIVVAVKKLKRESLQGYKEWLAEVNYLGQLRHENLVRLIGYCSESDNRLLVYEYM 180

Query: 515  SKGSLENHLFRKGVEPMPWPTRIRIATDVARGLSFLHNLDANVIYRDLKASNILLDSDFN 694
             KGSLENHLFRKGV P+ W  R+ IA DVARGL+FLH+ + NVIYRDLKASNILLDS+FN
Sbjct: 181  PKGSLENHLFRKGVTPISWRVRMDIAVDVARGLAFLHSSEPNVIYRDLKASNILLDSEFN 240

Query: 695  AKLSDFGLARDGPTGDKTHVSTRVVGTKGYAAPEYVATGRLTPKSDVYSFGVVLLELLSG 874
            AKLSDFGLAR+GPTGDKTHVSTRV+GT+GYAAPEYVATG LTPKSDVYSFGVVLLELLSG
Sbjct: 241  AKLSDFGLAREGPTGDKTHVSTRVMGTRGYAAPEYVATGHLTPKSDVYSFGVVLLELLSG 300

Query: 875  RRALGDERAGGVDEETLVDWAKPLLGE-KRLLRIMDSRMGGQYSKKEAQAVASLAYLCLN 1051
            +RAL  E+ G V EETLVDW KPLL + KR+LRIMD+RMGGQYS+KEAQA ASLA  CL+
Sbjct: 301  KRALDHEKVGRV-EETLVDWGKPLLSDGKRMLRIMDTRMGGQYSRKEAQAAASLALNCLH 359

Query: 1052 FDPKIRPSMLEVVGKLEQIPLAKNVSRT 1135
             DPK RPSM EV+ +LE++  AK++  T
Sbjct: 360  TDPKNRPSMAEVLDELERLHTAKDILGT 387


>ref|XP_002313478.1| predicted protein [Populus trichocarpa] gi|222849886|gb|EEE87433.1|
            predicted protein [Populus trichocarpa]
          Length = 340

 Score =  473 bits (1218), Expect = e-131
 Identities = 233/328 (71%), Positives = 282/328 (85%), Gaps = 3/328 (0%)
 Frame = +2

Query: 188  EITVSNNLRAFSYHDLKNAAKNFRSDSLLGEGGFGWVFKAWLDENSFGPSKPGAGISVAI 367
            +++  +NL++FS+ DLKNA KNFRS++LLGEGGFG VFK W+D N+F P+KPG+G+ VA+
Sbjct: 1    DVSAHSNLKSFSFTDLKNATKNFRSETLLGEGGFGCVFKGWIDLNTFAPTKPGSGVIVAV 60

Query: 368  KKLKTGSCQGHREWLTEVNYLGQLHHQNLVKLIGYCFEADNRLLVYEFMSKGSLENHLFR 547
            KKLK  SCQGH+EWLTEV YLGQLHH+NLVKLIGYC E+DNRLLVYEFM KGSLE HLFR
Sbjct: 61   KKLKPESCQGHKEWLTEVTYLGQLHHENLVKLIGYCSESDNRLLVYEFMPKGSLEQHLFR 120

Query: 548  KGVEPMPWPTRIRIATDVARGLSFLHNLDANVIYRDLKASNILLDSDFNAKLSDFGLARD 727
            KGV+P+ W  R+ IA DVARGLSFLH LDANVIYRDLKASN+LLDSD+NAKLSDFGLARD
Sbjct: 121  KGVQPITWTMRMNIAIDVARGLSFLHGLDANVIYRDLKASNVLLDSDYNAKLSDFGLARD 180

Query: 728  GPTGDKTHVSTRVVGTKGYAAPEYVAT-GRLTPKSDVYSFGVVLLELLSGRRALGDERAG 904
            GPTGD THVST+V+GT+GYAAPEYVAT G LTPKSDVYS+GVVLLELLSGRRA+ +ER G
Sbjct: 181  GPTGDNTHVSTKVLGTRGYAAPEYVATAGHLTPKSDVYSYGVVLLELLSGRRAMDEER-G 239

Query: 905  GVDEETLVDWAKP-LLGEKRLLRIMDSRMGGQYSKKEAQAVASLAYLCLNFDPKIRPSML 1081
            G D+ETLVDWAKP L+  +R+LRIMD+R+GGQY KK AQA A+LA  CL+ DPK RP M+
Sbjct: 240  GFDDETLVDWAKPFLIDSRRVLRIMDTRLGGQYPKKAAQAAAALALQCLHTDPKNRPPMI 299

Query: 1082 EVVGKLEQIPLAKNVSRTSQ-VKQEHIG 1162
            +V+  LE++  +K++ RT++ VK ++ G
Sbjct: 300  DVLTTLEKLITSKDIPRTARPVKLDNHG 327