BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00016995
         (1856 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002265193.1| PREDICTED: cleavage stimulation factor subun...   637   e-180
ref|XP_002303484.1| predicted protein [Populus trichocarpa] gi|2...   629   e-178
ref|XP_002519397.1| plant RNA cleavage stimulation factor, putat...   609   e-172
ref|XP_004144686.1| PREDICTED: cleavage stimulation factor subun...   606   e-171
ref|XP_003555072.1| PREDICTED: cleavage stimulation factor subun...   591   e-166

>ref|XP_002265193.1| PREDICTED: cleavage stimulation factor subunit 3 [Vitis vinifera]
            gi|297736046|emb|CBI24084.3| unnamed protein product
            [Vitis vinifera]
          Length = 769

 Score =  637 bits (1643), Expect = e-180
 Identities = 335/543 (61%), Positives = 384/543 (70%), Gaps = 11/543 (2%)
 Frame = -3

Query: 1842 KKKYVDVIDWNMLAVPPSGANKEELQWVAWKRFLAFEKENPQRVDSTSANKRVVVTYDQC 1663
            +KKYVD IDWNMLAVPP+G +KEE+QW+AWK+FLAFEK NPQR+DS S+NKR++ TY+QC
Sbjct: 218  QKKYVDEIDWNMLAVPPTGTSKEEMQWMAWKKFLAFEKGNPQRIDSNSSNKRILYTYEQC 277

Query: 1662 LMYLYHYPDIWYDYATWHAKNGAVDSAIKVFHRALKALPDSXXXXXXXXXXXESRGAIQS 1483
            LMYLYHYPDIWYDYATWHA+NG++D+AIKVF RA KALPDS           ESRGAIQ 
Sbjct: 278  LMYLYHYPDIWYDYATWHARNGSIDAAIKVFQRASKALPDSDMLRYAYAELEESRGAIQP 337

Query: 1482 AKKIYEGLLKDAANTTALSHIQFIRFLRRTEGVEAARRYFSAVRNSPGCPYQVYVAYAMV 1303
            AKKIYE LL D  N TAL HIQFIRFLRRTEGVEAAR+YF   R SP C Y V+VAYAM+
Sbjct: 338  AKKIYESLLGDGVNATALVHIQFIRFLRRTEGVEAARKYFLDARKSPNCTYHVFVAYAMM 397

Query: 1302 AFCLDKDAKVAHNVFELGLKNYMREPEYILEYANFLSRLNDDRNIRALFERALSSLPPEK 1123
            AFCLDKD KVAHNVFE GLK +M EP YILEYA+FLSRLNDDRNIRALFERALSSLPP++
Sbjct: 398  AFCLDKDPKVAHNVFEAGLKRFMHEPGYILEYADFLSRLNDDRNIRALFERALSSLPPDE 457

Query: 1122 SVEVWKKFTQFEQTYGDLASMLKVEQRRKEALSKTGEEGASALESSLQDVVSRYSFMDLW 943
            SVEVWK+FTQFEQTYGDLASMLKVEQRRKEALS+TGE+G +ALESSLQDVVSRYSFMDLW
Sbjct: 458  SVEVWKRFTQFEQTYGDLASMLKVEQRRKEALSRTGEDGTTALESSLQDVVSRYSFMDLW 517

Query: 942  PCSSNDLDNLVRQEWLXXXXXXXXXXXSLANGAVLIDKGSSGLATDSNPSTKIIYPDTSR 763
            PCSS DLD+L RQEWL           ++  G    +K +SG  T+SNP+TK+ YPDTS+
Sbjct: 518  PCSSRDLDHLARQEWLAKNINKKVEKSAILKGVGSTEKSASGFTTNSNPATKVFYPDTSQ 577

Query: 762  MVVYDPRQKPG----PSPPEXXXXXXXXXXXXXXXVGAANSP----NDIXXXXXXXXXXX 607
            MVVYDPRQKPG    PS                     ++ P    ++I           
Sbjct: 578  MVVYDPRQKPGTGALPSTTAPVLPSISGTLSNPSVPMVSSRPANPLDEILKSTPPALVAF 637

Query: 606  XXXXXAVEGPSPDVDFVLSICMQSNIPVKQVGKSGTTSKHMQGVAAPXXXXXXXXXXSHL 427
                 AVEGPSPDVD VLSIC+QSN+   Q G     S  +     P          SH 
Sbjct: 638  IANLPAVEGPSPDVDVVLSICLQSNVSTGQTG----LSTQLAAGPVPSTSDLSGSSKSHP 693

Query: 426  VPNNSSYKP--NRQXXXXXXXXXXXXXXXTSVQSQPLPKDVFKLRQMRKAR-ATGSQTGS 256
            VP+ SS+KP  +RQ                + QS PLP+DVFK+RQ+RKAR  T SQTGS
Sbjct: 694  VPSGSSFKPMRDRQPGKRKDLDRQEDDETATAQSLPLPRDVFKIRQIRKARGGTTSQTGS 753

Query: 255  ASY 247
            ASY
Sbjct: 754  ASY 756


>ref|XP_002303484.1| predicted protein [Populus trichocarpa] gi|222840916|gb|EEE78463.1|
            predicted protein [Populus trichocarpa]
          Length = 769

 Score =  629 bits (1622), Expect = e-178
 Identities = 328/538 (60%), Positives = 391/538 (72%), Gaps = 6/538 (1%)
 Frame = -3

Query: 1842 KKKYVDVIDWNMLAVPPSGANKEELQWVAWKRFLAFEKENPQRVDSTSANKRVVVTYDQC 1663
            +KKYVD ID+NMLAVPP+G+ KEE QW+AWKRFL FEK NPQR+DS S+NKR++ TY+QC
Sbjct: 221  QKKYVDEIDYNMLAVPPTGSFKEEQQWMAWKRFLTFEKGNPQRIDSVSSNKRIIFTYEQC 280

Query: 1662 LMYLYHYPDIWYDYATWHAKNGAVDSAIKVFHRALKALPDSXXXXXXXXXXXESRGAIQS 1483
            LMYLYHY D+WYDYATWHAK+G++DSAIKVF RALKALPDS           ESRGAIQ 
Sbjct: 281  LMYLYHYQDVWYDYATWHAKSGSIDSAIKVFQRALKALPDSDTLKYAYAELEESRGAIQP 340

Query: 1482 AKKIYEGLLKDAANTTALSHIQFIRFLRRTEGVEAARRYFSAVRNSPGCPYQVYVAYAMV 1303
            A+KIYE LL D  N TAL+HIQFIRFLRR EGVEAAR+YF   R SP C Y VYVAYA++
Sbjct: 341  ARKIYESLLGDGVNATALAHIQFIRFLRRNEGVEAARKYFLDARKSPDCSYHVYVAYALI 400

Query: 1302 AFCLDKDAKVAHNVFELGLKNYMREPEYILEYANFLSRLNDDRNIRALFERALSSLPPEK 1123
            AFCLDKD+K+AHN+FE GLK +M EP YILEYA+FLSRLND+RNIRALFERALSSLPPE+
Sbjct: 401  AFCLDKDSKIAHNIFEAGLKRFMHEPVYILEYADFLSRLNDERNIRALFERALSSLPPEE 460

Query: 1122 SVEVWKKFTQFEQTYGDLASMLKVEQRRKEALSKTGEEGASALESSLQDVVSRYSFMDLW 943
            SVEVWK++ QFEQTYGDLASMLKVEQRRKEALS+TGE+GASALESSLQDVVSRYSFMDLW
Sbjct: 461  SVEVWKRYIQFEQTYGDLASMLKVEQRRKEALSRTGEDGASALESSLQDVVSRYSFMDLW 520

Query: 942  PCSSNDLDNLVRQEWLXXXXXXXXXXXSLANGAVLIDKGSSGLATDSNPSTKIIYPDTSR 763
            PCSS DLD+L RQEWL           +++NG   +DK  +GLA++SN S K+IYPDTS+
Sbjct: 521  PCSSKDLDHLARQEWLAKNINKKAEKSAVSNGPATLDKIPAGLASNSNVSGKVIYPDTSQ 580

Query: 762  MVVYDPRQK--PGPSPPEXXXXXXXXXXXXXXXVG-AANSPNDIXXXXXXXXXXXXXXXX 592
             V+YDPRQK   G  P                 +G A N  +++                
Sbjct: 581  TVIYDPRQKLEAGIPPSTTASGFKAASNPLSNPIGLAPNVFDEVLKATPPALISFLANLP 640

Query: 591  AVEGPSPDVDFVLSICMQSNIPVKQVGKSGTTSKHMQGVAAPXXXXXXXXXXSHLVPNNS 412
             VEGP+P+VD VLSIC+QS++PV + GKSGTT   M  ++ P          S  VP+ S
Sbjct: 641  VVEGPAPNVDIVLSICLQSDVPVGKTGKSGTTQTPM--LSGPATSDLSGSSRSRPVPSGS 698

Query: 411  SYKP-NRQXXXXXXXXXXXXXXXTSVQSQPLPKDVFKLRQMRKAR--ATGSQTGSASY 247
            S+K  +RQ                +VQSQPLP+DVF++RQ++K+R  AT SQTGS SY
Sbjct: 699  SFKTRDRQSGKRKDRDRQEEDETATVQSQPLPRDVFRIRQIQKSRAAATTSQTGSVSY 756


>ref|XP_002519397.1| plant RNA cleavage stimulation factor, putative [Ricinus communis]
            gi|223541464|gb|EEF43014.1| plant RNA cleavage
            stimulation factor, putative [Ricinus communis]
          Length = 767

 Score =  609 bits (1571), Expect = e-172
 Identities = 321/542 (59%), Positives = 377/542 (69%), Gaps = 10/542 (1%)
 Frame = -3

Query: 1842 KKKYVDVIDWNMLAVPPSGANKEELQWVAWKRFLAFEKENPQRVDSTSANKRVVVTYDQC 1663
            +KKYVD IDWN+LAVPP+G+ KEELQW+AWKRFLAFEK NPQR+DS S+NKR++ TY+QC
Sbjct: 219  RKKYVDDIDWNLLAVPPTGSYKEELQWMAWKRFLAFEKGNPQRIDSVSSNKRIIFTYEQC 278

Query: 1662 LMYLYHYPDIWYDYATWHAKNGAVDSAIKVFHRALKALPDSXXXXXXXXXXXESRGAIQS 1483
            LMYLYHYPDIWYDYATWHAK G++D+AIKVF RALKALPDS           ESRGAIQ 
Sbjct: 279  LMYLYHYPDIWYDYATWHAKGGSIDAAIKVFQRALKALPDSEMLKYAYAELEESRGAIQP 338

Query: 1482 AKKIYEGLLKDAANTTALSHIQFIRFLRRTEGVEAARRYFSAVRNSPGCPYQVYVAYAMV 1303
            AKKIYE LL D  N TAL+HIQFIRFLRR EGVEAAR+YF   R SP C Y VYVAYA++
Sbjct: 339  AKKIYETLLGDGVNATALAHIQFIRFLRRNEGVEAARKYFLDARKSPNCTYHVYVAYALM 398

Query: 1302 AFCLDKDAKVAHNVFELGLKNYMREPEYILEYANFLSRLNDDRNIRALFERALSSLPPEK 1123
            AFCLDKD K+AHNVFE GLK +M EP YILEYA+FLSRLNDD+NIRALFERALSSLPPE+
Sbjct: 399  AFCLDKDPKMAHNVFEAGLKRFMHEPVYILEYADFLSRLNDDKNIRALFERALSSLPPEE 458

Query: 1122 SVEVWKKFTQFEQTYGDLASMLKVEQRRKEALSKTGEEGASALESSLQDVVSRYSFMDLW 943
            SVEVWK+FTQFEQTYGDLASMLKVEQRRKEALS+TGE+GASALE SLQDV SRYSFMDLW
Sbjct: 459  SVEVWKRFTQFEQTYGDLASMLKVEQRRKEALSRTGEDGASALEGSLQDVASRYSFMDLW 518

Query: 942  PCSSNDLDNLVRQEWLXXXXXXXXXXXSLANGAVLIDKGSSGLATDSNPSTKIIYPDTSR 763
            PCSS DLD+L RQEWL           +++NG  ++D+ S+GL ++S  S K+IYPDTS 
Sbjct: 519  PCSSKDLDHLARQEWLAKNISKKMEKSTISNGLGILDRVSTGLKSNSAVSAKVIYPDTSS 578

Query: 762  MVVYDPRQK-------PGPSPPEXXXXXXXXXXXXXXXVGAANSPNDIXXXXXXXXXXXX 604
            M +Y+PRQK          +                     AN+ ++I            
Sbjct: 579  MAIYEPRQKHEVGISLSTTATGFGSASNPSSNTIVGLVGSGANAFDEILKATPPALISFL 638

Query: 603  XXXXAVEGPSPDVDFVLSICMQSNIPVKQVGKSGTTSKHMQGVAAPXXXXXXXXXXSHLV 424
                 VEGP+P+VD VLSIC+QS +   Q+GK GT+       A P          S   
Sbjct: 639  STLPTVEGPTPNVDIVLSICLQSELTNGQMGKLGTSP------AVPAPPAPATSDLSGSS 692

Query: 423  PNNSSYKP--NRQXXXXXXXXXXXXXXXTSVQSQPLPKDVFKLRQMRKAR-ATGSQTGSA 253
             +    KP  +RQ                +VQSQPLP+D+F++R  +KAR  T SQTGSA
Sbjct: 693  KSRPVLKPSRDRQSGKRKDIERQEEDETATVQSQPLPRDIFRIRHSQKARVGTASQTGSA 752

Query: 252  SY 247
            SY
Sbjct: 753  SY 754


>ref|XP_004144686.1| PREDICTED: cleavage stimulation factor subunit 3-like [Cucumis
            sativus]
          Length = 871

 Score =  606 bits (1562), Expect = e-171
 Identities = 312/536 (58%), Positives = 378/536 (70%), Gaps = 4/536 (0%)
 Frame = -3

Query: 1842 KKKYVDVIDWNMLAVPPSGANKEELQWVAWKRFLAFEKENPQRVDSTSANKRVVVTYDQC 1663
            +KKYVD ID NMLAVPP+G++KEELQW++W+R +AFEK NPQR+DS S+NKR++ TY+QC
Sbjct: 323  RKKYVDEIDCNMLAVPPTGSSKEELQWMSWRRLIAFEKGNPQRIDSASSNKRIIFTYEQC 382

Query: 1662 LMYLYHYPDIWYDYATWHAKNGAVDSAIKVFHRALKALPDSXXXXXXXXXXXESRGAIQS 1483
            LMYLYHYPD+WYDYA WHA NG++D+AIKVF RALKALPDS           ESRG++QS
Sbjct: 383  LMYLYHYPDVWYDYAMWHASNGSIDAAIKVFQRALKALPDSDMLKFAYAELEESRGSLQS 442

Query: 1482 AKKIYEGLLKDAANTTALSHIQFIRFLRRTEGVEAARRYFSAVRNSPGCPYQVYVAYAMV 1303
            AKKIYE LL D  N TAL+HIQFIRFLRR EGVEAAR++F   R SP C Y VYVAYAM+
Sbjct: 443  AKKIYESLLSDGVNATALAHIQFIRFLRRNEGVEAARKHFLDARKSPNCTYHVYVAYAMM 502

Query: 1302 AFCLDKDAKVAHNVFELGLKNYMREPEYILEYANFLSRLNDDRNIRALFERALSSLPPEK 1123
            AFCLDKD K+AHNVFE G+K +M EP YIL+YA+FL+RLNDDRNIRALFERALS+LP E+
Sbjct: 503  AFCLDKDPKIAHNVFEDGMKRFMNEPTYILKYADFLARLNDDRNIRALFERALSTLPLEE 562

Query: 1122 SVEVWKKFTQFEQTYGDLASMLKVEQRRKEALSKTGEEGASALESSLQDVVSRYSFMDLW 943
            S EVWK+F  FEQTYGDLASMLKVE+RRKEALS+TGE+GAS LESSLQDVVSRYSFMDLW
Sbjct: 563  SAEVWKRFIHFEQTYGDLASMLKVEKRRKEALSQTGEDGASTLESSLQDVVSRYSFMDLW 622

Query: 942  PCSSNDLDNLVRQEWLXXXXXXXXXXXSLANGAVLIDKGSSGLATDSNPSTKIIYPDTSR 763
            PC+S+DLDNL RQEWL           SL  G   +D GS+G  + S PSTK++YPDTS+
Sbjct: 623  PCTSSDLDNLTRQEWLAKNISKNSEKSSLPGGTGFLDTGSAGFMSHSIPSTKVVYPDTSQ 682

Query: 762  MVVYDPRQKPGPSPPEXXXXXXXXXXXXXXXVGAANSP--NDIXXXXXXXXXXXXXXXXA 589
            MV+YDP Q  G  P                    A +   ++I                A
Sbjct: 683  MVIYDPSQILGILPTATASGLPANPSNPVSVASGAPTSVFDEILKATPAALIAFLANLPA 742

Query: 588  VEGPSPDVDFVLSICMQSNIPVKQVGKSGTTSKHMQGVAAPXXXXXXXXXXSHLVPNNS- 412
            V+GP+PDVD VLS+C++S++P   + KSG T   + G   P          SH   N+S 
Sbjct: 743  VDGPTPDVDIVLSVCLESDLPTVPLVKSGATPAQVSGGPVPTTSDLSGSSKSHAFSNSSL 802

Query: 411  SYKPNRQXXXXXXXXXXXXXXXTSVQSQPLPKDVFKLRQMRKAR-ATGSQTGSASY 247
             +  ++Q               T+VQSQP+PKD F++RQ++KAR AT SQTGSASY
Sbjct: 803  KHTRDKQSGKRKDYDRQEDNESTTVQSQPMPKDFFRIRQIQKARGATSSQTGSASY 858


>ref|XP_003555072.1| PREDICTED: cleavage stimulation factor subunit 3-like [Glycine max]
          Length = 832

 Score =  591 bits (1524), Expect = e-166
 Identities = 319/548 (58%), Positives = 375/548 (68%), Gaps = 16/548 (2%)
 Frame = -3

Query: 1842 KKKYVDVIDWNMLAVPPSGANKEELQWVAWKRFLAFEKENPQRVDSTSANKRVVVTYDQC 1663
            +KKYVD IDWNMLAVPP+G+ KEE+QW+AWKR L+FEK NPQR+D+ S+NKR++ TY+QC
Sbjct: 277  RKKYVDEIDWNMLAVPPTGSYKEEMQWMAWKRLLSFEKGNPQRIDTASSNKRIIFTYEQC 336

Query: 1662 LMYLYHYPDIWYDYATWHAKNGAVDSAIKVFHRALKALPDSXXXXXXXXXXXESRGAIQS 1483
            LM++YHYPDIWYDYATWHAK G +DSAIKVF RALKALPDS           ESRGAIQ+
Sbjct: 337  LMHMYHYPDIWYDYATWHAKGGLIDSAIKVFQRALKALPDSEMLRYAYAELEESRGAIQA 396

Query: 1482 AKKIYEGLLKDAANTTALSHIQFIRFLRRTEGVEAARRYFSAVRNSPGCPYQVYVAYAMV 1303
            AKKIYE ++ D  + T LSHIQFIRFLRRTEGVEAAR+YF   R SP C Y VYVAYA +
Sbjct: 397  AKKIYESVMGDGDSATTLSHIQFIRFLRRTEGVEAARKYFLDARKSPSCTYHVYVAYATM 456

Query: 1302 AFCLDKDAKVAHNVFELGLKNYMREPEYILEYANFLSRLNDDRNIRALFERALSSLPPEK 1123
            AFCLDKD K+AHNVFE GLK +M EP YILEYA+FL RLNDD+NIRALFERALSSLPPE+
Sbjct: 457  AFCLDKDPKMAHNVFEAGLKRFMHEPVYILEYADFLIRLNDDQNIRALFERALSSLPPEE 516

Query: 1122 SVEVWKKFTQFEQTYGDLASMLKVEQRRKEALSKTGEEGASALESSLQDVVSRYSFMDLW 943
            SVEVWKKFT+FEQTYGDLASMLKVEQRRKEALS  G E  +ALESSLQD+VSRYSFMDLW
Sbjct: 517  SVEVWKKFTKFEQTYGDLASMLKVEQRRKEALS--GAEDGTALESSLQDIVSRYSFMDLW 574

Query: 942  PCSSNDLDNLVRQEWLXXXXXXXXXXXSLANGAVLIDKGSSGLATDSNPSTKIIYPDTSR 763
            PCSSNDLD+L RQ+WL            L NG  L+DK S  +A+ S   +KI+YPDTS+
Sbjct: 575  PCSSNDLDHLARQQWLAKNINKKVEKSILPNGTTLLDKTS--MASISTMPSKIVYPDTSK 632

Query: 762  MVVYDPRQKPGPSPPEXXXXXXXXXXXXXXXVGAANSPNDIXXXXXXXXXXXXXXXXAVE 583
            MV+YDP+  PG                     G  N+ ++I                AVE
Sbjct: 633  MVIYDPKHTPGRCGKPKEHLGPCLIQLLVAGAG-TNAFDEILKATPPALVSFLANLPAVE 691

Query: 582  GPSPDVDFVLSICMQSNIPVKQVGKSGTTSKHMQGVA------------APXXXXXXXXX 439
            GP P+VD VLSIC+QS++P  Q  K+G  ++   G A            A          
Sbjct: 692  GPMPNVDIVLSICLQSDLPTGQSVKTGIPTQVQSGKAGIPALLPAGSAPAAAASELSGSS 751

Query: 438  XSHLVPNNS-SYKP--NRQXXXXXXXXXXXXXXXTSVQSQPLPKDVFKLRQMRKARA-TG 271
             SH  P+   S KP  NRQ               T+VQSQPLP+D F++RQ +KARA + 
Sbjct: 752  KSHPAPSGGVSLKPGSNRQYGKRKEPDRQDEDDTTTVQSQPLPRDAFRIRQYQKARASSA 811

Query: 270  SQTGSASY 247
            SQTGS SY
Sbjct: 812  SQTGSVSY 819