BLASTX nr result
ID: Bupleurum21_contig00016959
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00016959
(1718 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002282185.1| PREDICTED: cytochrome P450 86B1 [Vitis vinif... 358 e-178
emb|CAN77648.1| hypothetical protein VITISV_032391 [Vitis vinifera] 357 e-177
ref|XP_002264277.2| PREDICTED: cytochrome P450 86B1-like [Vitis ... 349 e-173
emb|CAN63571.1| hypothetical protein VITISV_008250 [Vitis vinifera] 349 e-173
ref|XP_002336104.1| cytochrome P450 [Populus trichocarpa] gi|222... 346 e-168
>ref|XP_002282185.1| PREDICTED: cytochrome P450 86B1 [Vitis vinifera]
Length = 526
Score = 358 bits (919), Expect(3) = e-178
Identities = 173/254 (68%), Positives = 207/254 (81%), Gaps = 2/254 (0%)
Frame = -3
Query: 1425 GIVTSDPSKIEYMLKSKFNNFPKGKYYRERFHELLGEGIFNADDQSWKHQRQAAASVMHS 1246
GI+T DP+ IEYMLK++F NFPKG YYRERF++LLG GIFNADD+SWK QR+ A MHS
Sbjct: 90 GIMTIDPANIEYMLKTRFKNFPKGNYYRERFNDLLGGGIFNADDESWKEQRRLATFEMHS 149
Query: 1245 VKFVEYSTQSMHDLVHEKLLKVLEDVVKSKICIDLQELLLRFTFDNICVAAFGVDPGCLS 1066
FV +S Q++ LVH+KLLK++E + KS CIDLQE+LLRFTFDNIC AAFGVDPGCL+
Sbjct: 150 GPFVAHSFQTIQGLVHQKLLKLIEKLAKSGDCIDLQEVLLRFTFDNICTAAFGVDPGCLA 209
Query: 1065 IGLPDVPFAKAFEEATESTLCRFMMPPFIWKTLRFFELGFERRLKESVKIVHEFAEKTVR 886
+ LP+VPFAKAFEEATESTL RF++PPF+WK +RFF +G E+RLKE+V+IVH+FAEKTV
Sbjct: 210 LDLPEVPFAKAFEEATESTLFRFIVPPFVWKPMRFFRVGTEKRLKEAVRIVHDFAEKTVT 269
Query: 885 ERKKELSLHDQNNEKSDLLSRL--INLESAGKNGNFSDKLLKDFCISFILAGRDTSSVGL 712
ER+ ELS + + DLLSR+ I GKN NFSDK LKDFCISFILAGRDTSSV L
Sbjct: 270 ERRIELSKAGSSTNRCDLLSRIVAIGYSEQGKNNNFSDKFLKDFCISFILAGRDTSSVAL 329
Query: 711 AWFFWLLSQNPQVE 670
AWFFWL+ +NP VE
Sbjct: 330 AWFFWLIHKNPDVE 343
Score = 243 bits (620), Expect(3) = e-178
Identities = 108/142 (76%), Positives = 131/142 (92%)
Frame = -2
Query: 565 LQAALSESLRLYPPVPLDFKEVLEDDIFPDGTPVQSGAKVLYNIYSMARMESVWGQDCLE 386
LQAAL+ESLRLYP VP+DFKEV+EDD+FPDGTP++ GA+VLY+I+SMAR+ES+WG+DC+E
Sbjct: 380 LQAALTESLRLYPSVPIDFKEVMEDDVFPDGTPIKRGARVLYSIFSMARIESIWGKDCME 439
Query: 385 FKPERWIKDGEFVSENPFKYAVFNGGPRLCVGKKFAYMQMKMVTASVILRYSIRVVDGFE 206
FKPERWIKDGE VSEN FKY VFN GPRLC+GKKFAYMQMKMV AS+++RYS++VV+G
Sbjct: 440 FKPERWIKDGELVSENQFKYPVFNAGPRLCIGKKFAYMQMKMVAASILMRYSVKVVEGHN 499
Query: 205 VVPKMTTTLYMKNGLMVYLEPR 140
V+PKMTTTLYMKNGL+V +PR
Sbjct: 500 VIPKMTTTLYMKNGLLVTFKPR 521
Score = 73.2 bits (178), Expect(3) = e-178
Identities = 30/64 (46%), Positives = 48/64 (75%)
Frame = -2
Query: 1675 SALTWLTDNLHLSDLAFSLLCLFIFSSINQKLRSKGPMLWPLLGNLPSMVMHMNNLYDWM 1496
SA+ W+ ++ SD+A +L +FIFSSI +L +KGPMLWP++G +P++ HMN++Y+W
Sbjct: 8 SAVDWVVHHIWFSDIAVALSSIFIFSSILHRLTNKGPMLWPVMGIIPTVFFHMNDIYNWG 67
Query: 1495 TKSL 1484
T+ L
Sbjct: 68 TRVL 71
>emb|CAN77648.1| hypothetical protein VITISV_032391 [Vitis vinifera]
Length = 526
Score = 357 bits (917), Expect(3) = e-177
Identities = 173/254 (68%), Positives = 207/254 (81%), Gaps = 2/254 (0%)
Frame = -3
Query: 1425 GIVTSDPSKIEYMLKSKFNNFPKGKYYRERFHELLGEGIFNADDQSWKHQRQAAASVMHS 1246
GI+T DP+ IEYMLK++F NFPKG YYRERF++LLG GIFNADD+SWK QR+ A MHS
Sbjct: 90 GIMTIDPANIEYMLKTRFKNFPKGNYYRERFNDLLGGGIFNADDESWKEQRRLATFEMHS 149
Query: 1245 VKFVEYSTQSMHDLVHEKLLKVLEDVVKSKICIDLQELLLRFTFDNICVAAFGVDPGCLS 1066
FV +S Q++ LVH+KLLK++E + KS CIDLQE+LLRFTFDNIC AAFGVDPGCL+
Sbjct: 150 GPFVAHSFQTIQGLVHQKLLKLIEKLXKSGDCIDLQEVLLRFTFDNICTAAFGVDPGCLA 209
Query: 1065 IGLPDVPFAKAFEEATESTLCRFMMPPFIWKTLRFFELGFERRLKESVKIVHEFAEKTVR 886
+ LP+VPFAKAFEEATESTL RF++PPF+WK +RFF +G E+RLKE+V+IVH+FAEKTV
Sbjct: 210 LDLPEVPFAKAFEEATESTLFRFIVPPFVWKPMRFFRVGTEKRLKEAVRIVHDFAEKTVT 269
Query: 885 ERKKELSLHDQNNEKSDLLSRL--INLESAGKNGNFSDKLLKDFCISFILAGRDTSSVGL 712
ER+ ELS + + DLLSR+ I GKN NFSDK LKDFCISFILAGRDTSSV L
Sbjct: 270 ERRIELSKAGSSTNRCDLLSRIVAIGYSEQGKNNNFSDKFLKDFCISFILAGRDTSSVAL 329
Query: 711 AWFFWLLSQNPQVE 670
AWFFWL+ +NP VE
Sbjct: 330 AWFFWLIHKNPXVE 343
Score = 243 bits (620), Expect(3) = e-177
Identities = 108/142 (76%), Positives = 131/142 (92%)
Frame = -2
Query: 565 LQAALSESLRLYPPVPLDFKEVLEDDIFPDGTPVQSGAKVLYNIYSMARMESVWGQDCLE 386
LQAAL+ESLRLYP VP+DFKEV+EDD+FPDGTP++ GA+VLY+I+SMAR+ES+WG+DC+E
Sbjct: 380 LQAALTESLRLYPSVPIDFKEVMEDDVFPDGTPIKRGARVLYSIFSMARIESIWGKDCME 439
Query: 385 FKPERWIKDGEFVSENPFKYAVFNGGPRLCVGKKFAYMQMKMVTASVILRYSIRVVDGFE 206
FKPERWIKDGE VSEN FKY VFN GPRLC+GKKFAYMQMKMV AS+++RYS++VV+G
Sbjct: 440 FKPERWIKDGELVSENQFKYPVFNAGPRLCIGKKFAYMQMKMVAASILMRYSVKVVEGHN 499
Query: 205 VVPKMTTTLYMKNGLMVYLEPR 140
V+PKMTTTLYMKNGL+V +PR
Sbjct: 500 VIPKMTTTLYMKNGLLVTFKPR 521
Score = 70.9 bits (172), Expect(3) = e-177
Identities = 29/64 (45%), Positives = 47/64 (73%)
Frame = -2
Query: 1675 SALTWLTDNLHLSDLAFSLLCLFIFSSINQKLRSKGPMLWPLLGNLPSMVMHMNNLYDWM 1496
SA+ W+ ++ SD+A +L +FIFSSI +L +KGPMLWP++G +P++ H N++Y+W
Sbjct: 8 SAVDWVVHHIWFSDIAVALSSIFIFSSILHRLTNKGPMLWPVMGIIPTVFFHXNDIYNWG 67
Query: 1495 TKSL 1484
T+ L
Sbjct: 68 TRVL 71
>ref|XP_002264277.2| PREDICTED: cytochrome P450 86B1-like [Vitis vinifera]
Length = 521
Score = 349 bits (895), Expect(3) = e-173
Identities = 166/254 (65%), Positives = 211/254 (83%), Gaps = 2/254 (0%)
Frame = -3
Query: 1425 GIVTSDPSKIEYMLKSKFNNFPKGKYYRERFHELLGEGIFNADDQSWKHQRQAAASVMHS 1246
GI+T DPS IE+MLK++F NFPKG YYRERFH+LLG GIFN D +SWK QR+ A+S MHS
Sbjct: 90 GIMTVDPSNIEFMLKTRFKNFPKGNYYRERFHDLLGGGIFNVDHESWKEQRRIASSEMHS 149
Query: 1245 VKFVEYSTQSMHDLVHEKLLKVLEDVVKSKICIDLQELLLRFTFDNICVAAFGVDPGCLS 1066
+FV YS Q++ DLV++KLL++ + + KS CIDLQE+LLRFTFDNIC AAFG+DPGCL+
Sbjct: 150 TQFVAYSFQTIQDLVNQKLLELTDKLAKSGDCIDLQEVLLRFTFDNICTAAFGIDPGCLA 209
Query: 1065 IGLPDVPFAKAFEEATESTLCRFMMPPFIWKTLRFFELGFERRLKESVKIVHEFAEKTVR 886
+ LP+V FAKAFEEATE TL RFM+PPFIWK+++FF +G E+RL+E+V++VH+FAEKTV
Sbjct: 210 LELPEVSFAKAFEEATELTLFRFMVPPFIWKSMKFFGVGTEKRLQEAVRVVHDFAEKTVA 269
Query: 885 ERKKELSLHDQNNEKSDLLSRL--INLESAGKNGNFSDKLLKDFCISFILAGRDTSSVGL 712
+R+ ELS N+++DLLSR+ I GK+ +FSDK LKDFCISFILAGRDTSSV L
Sbjct: 270 DRRIELSKTGNLNKQTDLLSRIMAIGEHEEGKDNHFSDKFLKDFCISFILAGRDTSSVAL 329
Query: 711 AWFFWLLSQNPQVE 670
AWFFWL+++NP+VE
Sbjct: 330 AWFFWLINKNPEVE 343
Score = 236 bits (602), Expect(3) = e-173
Identities = 106/142 (74%), Positives = 130/142 (91%)
Frame = -2
Query: 565 LQAALSESLRLYPPVPLDFKEVLEDDIFPDGTPVQSGAKVLYNIYSMARMESVWGQDCLE 386
LQAALSE+LRLYP VP+DFKEV+EDD+ PDGT V+ G++VLY+I+SMARMES+WG+DC+E
Sbjct: 374 LQAALSETLRLYPSVPVDFKEVVEDDVLPDGTRVKKGSRVLYSIFSMARMESIWGKDCME 433
Query: 385 FKPERWIKDGEFVSENPFKYAVFNGGPRLCVGKKFAYMQMKMVTASVILRYSIRVVDGFE 206
FKPERWIKDG+FVSEN FKY VFN GPRLC+GKKFA+ QMKMV AS+++RY+++VV+G
Sbjct: 434 FKPERWIKDGQFVSENQFKYPVFNAGPRLCIGKKFAFTQMKMVAASILMRYTVKVVEGHS 493
Query: 205 VVPKMTTTLYMKNGLMVYLEPR 140
VVPKMTTTLYM+NGL+V LEPR
Sbjct: 494 VVPKMTTTLYMRNGLLVTLEPR 515
Score = 73.9 bits (180), Expect(3) = e-173
Identities = 32/64 (50%), Positives = 46/64 (71%)
Frame = -2
Query: 1675 SALTWLTDNLHLSDLAFSLLCLFIFSSINQKLRSKGPMLWPLLGNLPSMVMHMNNLYDWM 1496
S + WL ++ SD+A +L +FI S I +L +KGPMLWP+LG LPS+ HMN++YDW
Sbjct: 8 SVVKWLCHHIWFSDIAVALSIVFILSCILNRLTNKGPMLWPVLGILPSLFFHMNDIYDWG 67
Query: 1495 TKSL 1484
T++L
Sbjct: 68 TRAL 71
>emb|CAN63571.1| hypothetical protein VITISV_008250 [Vitis vinifera]
Length = 521
Score = 349 bits (895), Expect(3) = e-173
Identities = 166/254 (65%), Positives = 211/254 (83%), Gaps = 2/254 (0%)
Frame = -3
Query: 1425 GIVTSDPSKIEYMLKSKFNNFPKGKYYRERFHELLGEGIFNADDQSWKHQRQAAASVMHS 1246
GI+T DPS IE+MLK++F NFPKG YYRERFH+LLG GIFN D +SWK QR+ A+S MHS
Sbjct: 90 GIMTVDPSNIEFMLKTRFKNFPKGNYYRERFHDLLGGGIFNVDHESWKEQRRIASSEMHS 149
Query: 1245 VKFVEYSTQSMHDLVHEKLLKVLEDVVKSKICIDLQELLLRFTFDNICVAAFGVDPGCLS 1066
+FV YS Q++ DLV++KLL++ + + KS CIDLQE+LLRFTFDNIC AAFG+DPGCL+
Sbjct: 150 TQFVAYSFQTIQDLVNQKLLELTDKLAKSGDCIDLQEVLLRFTFDNICTAAFGIDPGCLA 209
Query: 1065 IGLPDVPFAKAFEEATESTLCRFMMPPFIWKTLRFFELGFERRLKESVKIVHEFAEKTVR 886
+ LP+V FAKAFEEATE TL RFM+PPFIWK+++FF +G E+RL+E+V++VH+FAEKTV
Sbjct: 210 LELPEVSFAKAFEEATELTLFRFMVPPFIWKSMKFFGVGTEKRLQEAVRVVHDFAEKTVA 269
Query: 885 ERKKELSLHDQNNEKSDLLSRL--INLESAGKNGNFSDKLLKDFCISFILAGRDTSSVGL 712
+R+ ELS N+++DLLSR+ I GK+ +FSDK LKDFCISFILAGRDTSSV L
Sbjct: 270 DRRIELSKTGNLNKQTDLLSRIMAIGEHEEGKDNHFSDKFLKDFCISFILAGRDTSSVAL 329
Query: 711 AWFFWLLSQNPQVE 670
AWFFWL+++NP+VE
Sbjct: 330 AWFFWLINKNPEVE 343
Score = 236 bits (602), Expect(3) = e-173
Identities = 106/142 (74%), Positives = 130/142 (91%)
Frame = -2
Query: 565 LQAALSESLRLYPPVPLDFKEVLEDDIFPDGTPVQSGAKVLYNIYSMARMESVWGQDCLE 386
LQAALSE+LRLYP VP+DFKEV+EDD+ PDGT V+ G++VLY+I+SMARMES+WG+DC+E
Sbjct: 374 LQAALSETLRLYPSVPVDFKEVVEDDVLPDGTRVKKGSRVLYSIFSMARMESIWGKDCME 433
Query: 385 FKPERWIKDGEFVSENPFKYAVFNGGPRLCVGKKFAYMQMKMVTASVILRYSIRVVDGFE 206
FKPERWIKDG+FVSEN FKY VFN GPRLC+GKKFA+ QMKMV AS+++RY+++VV+G
Sbjct: 434 FKPERWIKDGQFVSENQFKYPVFNAGPRLCIGKKFAFTQMKMVAASILMRYTVKVVEGHS 493
Query: 205 VVPKMTTTLYMKNGLMVYLEPR 140
VVPKMTTTLYM+NGL+V LEPR
Sbjct: 494 VVPKMTTTLYMRNGLLVTLEPR 515
Score = 73.9 bits (180), Expect(3) = e-173
Identities = 32/64 (50%), Positives = 46/64 (71%)
Frame = -2
Query: 1675 SALTWLTDNLHLSDLAFSLLCLFIFSSINQKLRSKGPMLWPLLGNLPSMVMHMNNLYDWM 1496
S + WL ++ SD+A +L +FI S I +L +KGPMLWP+LG LPS+ HMN++YDW
Sbjct: 8 SVVEWLCHHIWFSDIAVALSIVFILSCILNRLTNKGPMLWPVLGILPSLFFHMNDIYDWG 67
Query: 1495 TKSL 1484
T++L
Sbjct: 68 TRAL 71
>ref|XP_002336104.1| cytochrome P450 [Populus trichocarpa] gi|222872554|gb|EEF09685.1|
cytochrome P450 [Populus trichocarpa]
Length = 526
Score = 346 bits (887), Expect(3) = e-168
Identities = 164/254 (64%), Positives = 205/254 (80%), Gaps = 2/254 (0%)
Frame = -3
Query: 1425 GIVTSDPSKIEYMLKSKFNNFPKGKYYRERFHELLGEGIFNADDQSWKHQRQAAASVMHS 1246
GIVT+DPS IEYMLK+ F NFPKGKY+RERF +LLG+GIFNADD+ W+ QRQA + MHS
Sbjct: 90 GIVTADPSNIEYMLKTNFKNFPKGKYFRERFQDLLGDGIFNADDELWREQRQAVRAEMHS 149
Query: 1245 VKFVEYSTQSMHDLVHEKLLKVLEDVVKSKICIDLQELLLRFTFDNICVAAFGVDPGCLS 1066
+F+E+S ++M DLVH+KLLK+ +++V+S DLQE+LLRFTFDNIC AAFGVDPGCL+
Sbjct: 150 SRFIEHSLRTMKDLVHQKLLKLTDNLVRSGDSFDLQEVLLRFTFDNICSAAFGVDPGCLA 209
Query: 1065 IGLPDVPFAKAFEEATESTLCRFMMPPFIWKTLRFFELGFERRLKESVKIVHEFAEKTVR 886
+ +PDVPFAKAFE+ATE L RF+MPPFIWKT++FF +G+E+ LKE+V IVHEFAEKTV+
Sbjct: 210 LDIPDVPFAKAFEKATELILFRFLMPPFIWKTMKFFGIGYEKTLKEAVGIVHEFAEKTVK 269
Query: 885 ERKKELSLHDQNNEKSDLLSRLINLE--SAGKNGNFSDKLLKDFCISFILAGRDTSSVGL 712
R+ E+ H +SDLLSRLI +E GK F DK +D C++FILAGRDT+SV L
Sbjct: 270 GRRDEIRKHGSLCHQSDLLSRLIEIEYTGRGKEMQFPDKYFRDLCVNFILAGRDTTSVAL 329
Query: 711 AWFFWLLSQNPQVE 670
AWFFWL+ NPQVE
Sbjct: 330 AWFFWLVHSNPQVE 343
Score = 226 bits (576), Expect(3) = e-168
Identities = 101/144 (70%), Positives = 129/144 (89%)
Frame = -2
Query: 565 LQAALSESLRLYPPVPLDFKEVLEDDIFPDGTPVQSGAKVLYNIYSMARMESVWGQDCLE 386
LQAALSESLRLYP VP++ KEV++DD+ PDG+ V+ GA+V Y I++M RM+S+WG++CLE
Sbjct: 378 LQAALSESLRLYPSVPIEVKEVVQDDVLPDGSIVKKGARVFYCIFAMGRMDSIWGENCLE 437
Query: 385 FKPERWIKDGEFVSENPFKYAVFNGGPRLCVGKKFAYMQMKMVTASVILRYSIRVVDGFE 206
FKPERWIKDG+FVSEN F+YAVFN GPRLC+GKKFAY+QMKMV AS++LRYS++VV+G +
Sbjct: 438 FKPERWIKDGKFVSENQFRYAVFNAGPRLCLGKKFAYLQMKMVAASILLRYSVKVVEGHD 497
Query: 205 VVPKMTTTLYMKNGLMVYLEPRNL 134
+PKMTTTLYMKNGL+V L PR++
Sbjct: 498 AIPKMTTTLYMKNGLLVTLMPRSV 521
Score = 68.9 bits (167), Expect(3) = e-168
Identities = 29/66 (43%), Positives = 49/66 (74%)
Frame = -2
Query: 1681 LSSALTWLTDNLHLSDLAFSLLCLFIFSSINQKLRSKGPMLWPLLGNLPSMVMHMNNLYD 1502
LSSA W+ + D++ +LL LF+F IN +L +KGPMLWP+LG +P+M +H+N++ +
Sbjct: 6 LSSATQWIYTHSSFWDVSMALLGLFVFQCINDRLTNKGPMLWPVLGIIPTMFLHVNDMNN 65
Query: 1501 WMTKSL 1484
++T++L
Sbjct: 66 FVTRAL 71