BLASTX nr result
ID: Bupleurum21_contig00016907
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Bupleurum21_contig00016907
(1681 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenas... 621 0.0
emb|CBI20743.3| unnamed protein product [Vitis vinifera] 577 0.0
ref|XP_002308509.1| predicted protein [Populus trichocarpa] gi|2... 580 0.0
ref|NP_180194.2| 3beta-hydroxysteroid-dehydrogenase/decarboxylas... 578 0.0
ref|XP_002878951.1| 3beta-hydroxysteroid-dehydrogenase/decarboxy... 576 0.0
>ref|XP_002282161.2| PREDICTED: 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2-like [Vitis vinifera]
Length = 563
Score = 621 bits (1601), Expect(2) = 0.0
Identities = 304/360 (84%), Positives = 328/360 (91%)
Frame = +3
Query: 39 MGGEERWCVVTGGRGFAARHLVEMLLRYEMFSVRIADLGPSINLHPCEENGTLGEALRSG 218
M GE++WCVVTGGRGFAARHLVEML+R FSVRIADLGPSI L P EE G LGEAL+SG
Sbjct: 1 MEGEQKWCVVTGGRGFAARHLVEMLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSG 60
Query: 219 RAAYVSADLRDKSQVSKACEGAEVVFHMAAPDSSINNHQLHQSVNVQGTANVIEACVESK 398
RA YVSADLRDK+QV KAC+GAE VFHMAAPDSSINNHQLH SVNVQGT NVI+AC+E K
Sbjct: 61 RAEYVSADLRDKAQVLKACQGAEAVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQK 120
Query: 399 VKRLIYTSSASVVFDGVHGIYNGDESLPYPSKHNDSYSATKADGEALVIKANGVNGLLTC 578
VKRLIYTSS SVVFDGVHGI+NGDESLPYP KHNDSYSATKA+GEALVIK+NG NGLLTC
Sbjct: 121 VKRLIYTSSPSVVFDGVHGIFNGDESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTC 180
Query: 579 CIRPSSIFGPGDKLLVPSLVSAARAGKSKFIIGDGNNMYDFTYVENVAHAHICAERALAS 758
CIRPSSIFGPGDKLLVPSLV+AARAGKSKFIIGDGNNMYDFTYVENVAHAHICAE+ALAS
Sbjct: 181 CIRPSSIFGPGDKLLVPSLVNAARAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALAS 240
Query: 759 DGAVAQKAAGQAYFITNMEPIKFWEFMSLILGGLGYERPRIKVPVSVMMPIAHVVEAIYN 938
+G +A++AAGQAYFITNMEPIKFWEF+SLIL GLGYERPRIK+P VMMPIAH+VE Y
Sbjct: 241 EGKIAEQAAGQAYFITNMEPIKFWEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYK 300
Query: 939 VFAPYGMKVPQLTPSRIRLLSCTRTFDCSKANDRLGYTPIVPLQEGLTRTIESYSDLRAE 1118
+ APYGMKVPQLTPSRIRLLSC+RTF CSKA D+L YTPIV LQEGL RTI+SY DLRAE
Sbjct: 301 MLAPYGMKVPQLTPSRIRLLSCSRTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAE 360
Score = 166 bits (420), Expect(2) = 0.0
Identities = 87/157 (55%), Positives = 100/157 (63%)
Frame = +1
Query: 1210 TILVLFAIYFNFVASGYTIVTAVSKLLAVASILLFLHGKSPEKILGYTVEKVPESKFHLS 1389
T+LVL A Y NF+ASGYTIVTAVSKL VASI LF+HG PEKILGYTVEK+P S FH S
Sbjct: 395 TLLVLMAFYHNFIASGYTIVTAVSKLFLVASIFLFIHGNLPEKILGYTVEKIPPSNFHFS 454
Query: 1390 KEMSHKVSLSMASSWNYVVNGLKSLCCGNDWPXXXXXXXXXXXXXXXXXXXXXXXFTIVL 1569
+E SH+ +LS+ASSWN VN LKSLC G+DW F L
Sbjct: 455 EERSHRAALSVASSWNTAVNVLKSLCKGDDWMLFLKVVLSLLILSFLGAISLQSLFVTGL 514
Query: 1570 PLVFVAFFVYEKKEEEIDGLVLRIISIGSKLKSDAEK 1680
+ FVAF++YE KEEEID L +S KLKSD K
Sbjct: 515 LIAFVAFYIYENKEEEIDRLASEALSFSCKLKSDIAK 551
>emb|CBI20743.3| unnamed protein product [Vitis vinifera]
Length = 540
Score = 577 bits (1487), Expect(2) = 0.0
Identities = 284/337 (84%), Positives = 306/337 (90%)
Frame = +3
Query: 108 MLLRYEMFSVRIADLGPSINLHPCEENGTLGEALRSGRAAYVSADLRDKSQVSKACEGAE 287
ML+R FSVRIADLGPSI L P EE G LGEAL+SGRA YVSADLRDK+QV KAC+GAE
Sbjct: 1 MLIRSGRFSVRIADLGPSIKLEPSEEKGLLGEALQSGRAEYVSADLRDKAQVLKACQGAE 60
Query: 288 VVFHMAAPDSSINNHQLHQSVNVQGTANVIEACVESKVKRLIYTSSASVVFDGVHGIYNG 467
VFHMAAPDSSINNHQLH SVNVQGT NVI+AC+E KVKRLIYTSS SVVFDGVHGI+NG
Sbjct: 61 AVFHMAAPDSSINNHQLHHSVNVQGTKNVIDACIEQKVKRLIYTSSPSVVFDGVHGIFNG 120
Query: 468 DESLPYPSKHNDSYSATKADGEALVIKANGVNGLLTCCIRPSSIFGPGDKLLVPSLVSAA 647
DESLPYP KHNDSYSATKA+GEALVIK+NG NGLLTCCIRPSSIFGPGDKLLVPSLV+AA
Sbjct: 121 DESLPYPDKHNDSYSATKAEGEALVIKSNGTNGLLTCCIRPSSIFGPGDKLLVPSLVNAA 180
Query: 648 RAGKSKFIIGDGNNMYDFTYVENVAHAHICAERALASDGAVAQKAAGQAYFITNMEPIKF 827
RAGKSKFIIGDGNNMYDFTYVENVAHAHICAE+ALAS+G +A++AAGQAYFITNMEPIKF
Sbjct: 181 RAGKSKFIIGDGNNMYDFTYVENVAHAHICAEQALASEGKIAEQAAGQAYFITNMEPIKF 240
Query: 828 WEFMSLILGGLGYERPRIKVPVSVMMPIAHVVEAIYNVFAPYGMKVPQLTPSRIRLLSCT 1007
WEF+SLIL GLGYERPRIK+P VMMPIAH+VE Y + APYGMKVPQLTPSRIRLLSC+
Sbjct: 241 WEFVSLILEGLGYERPRIKIPALVMMPIAHMVEWTYKMLAPYGMKVPQLTPSRIRLLSCS 300
Query: 1008 RTFDCSKANDRLGYTPIVPLQEGLTRTIESYSDLRAE 1118
RTF CSKA D+L YTPIV LQEGL RTI+SY DLRAE
Sbjct: 301 RTFSCSKAKDQLSYTPIVSLQEGLRRTIDSYPDLRAE 337
Score = 166 bits (420), Expect(2) = 0.0
Identities = 87/157 (55%), Positives = 100/157 (63%)
Frame = +1
Query: 1210 TILVLFAIYFNFVASGYTIVTAVSKLLAVASILLFLHGKSPEKILGYTVEKVPESKFHLS 1389
T+LVL A Y NF+ASGYTIVTAVSKL VASI LF+HG PEKILGYTVEK+P S FH S
Sbjct: 372 TLLVLMAFYHNFIASGYTIVTAVSKLFLVASIFLFIHGNLPEKILGYTVEKIPPSNFHFS 431
Query: 1390 KEMSHKVSLSMASSWNYVVNGLKSLCCGNDWPXXXXXXXXXXXXXXXXXXXXXXXFTIVL 1569
+E SH+ +LS+ASSWN VN LKSLC G+DW F L
Sbjct: 432 EERSHRAALSVASSWNTAVNVLKSLCKGDDWMLFLKVVLSLLILSFLGAISLQSLFVTGL 491
Query: 1570 PLVFVAFFVYEKKEEEIDGLVLRIISIGSKLKSDAEK 1680
+ FVAF++YE KEEEID L +S KLKSD K
Sbjct: 492 LIAFVAFYIYENKEEEIDRLASEALSFSCKLKSDIAK 528
>ref|XP_002308509.1| predicted protein [Populus trichocarpa] gi|222854485|gb|EEE92032.1|
predicted protein [Populus trichocarpa]
Length = 558
Score = 580 bits (1494), Expect(2) = 0.0
Identities = 285/360 (79%), Positives = 316/360 (87%)
Frame = +3
Query: 39 MGGEERWCVVTGGRGFAARHLVEMLLRYEMFSVRIADLGPSINLHPCEENGTLGEALRSG 218
M GEERWCVVTGGRGFAARHLVEML++Y++FSVRIADL P I L E+NG LG AL+SG
Sbjct: 1 MSGEERWCVVTGGRGFAARHLVEMLIQYDLFSVRIADLEPQITLSTEEQNGALGHALKSG 60
Query: 219 RAAYVSADLRDKSQVSKACEGAEVVFHMAAPDSSINNHQLHQSVNVQGTANVIEACVESK 398
RA YVSADLRDK QV K EGAEVVFHMAAP+SS+N+ QLH SVNVQGT NVI+ACVE K
Sbjct: 61 RANYVSADLRDKPQVLKVIEGAEVVFHMAAPNSSVNSQQLHYSVNVQGTKNVIDACVELK 120
Query: 399 VKRLIYTSSASVVFDGVHGIYNGDESLPYPSKHNDSYSATKADGEALVIKANGVNGLLTC 578
VKRLIYTSSASVVFDG+HGI NGDESLPY K DSYSATKA+GEA ++KANG NGLLTC
Sbjct: 121 VKRLIYTSSASVVFDGIHGIINGDESLPYTDKPLDSYSATKAEGEAAILKANGTNGLLTC 180
Query: 579 CIRPSSIFGPGDKLLVPSLVSAARAGKSKFIIGDGNNMYDFTYVENVAHAHICAERALAS 758
+RPSSIFGPGD+L VPSLV+AARAGKSKF+IGDGNN+YDFTYVENVAHAHICA+RALAS
Sbjct: 181 SLRPSSIFGPGDRLFVPSLVAAARAGKSKFMIGDGNNIYDFTYVENVAHAHICADRALAS 240
Query: 759 DGAVAQKAAGQAYFITNMEPIKFWEFMSLILGGLGYERPRIKVPVSVMMPIAHVVEAIYN 938
+ VA+KAAGQAYFITNMEPIKFWEF SLIL GLGYERPRIK+P +MPIA V+E IY
Sbjct: 241 EQEVAEKAAGQAYFITNMEPIKFWEFTSLILEGLGYERPRIKIPAFAVMPIAQVIEWIYK 300
Query: 939 VFAPYGMKVPQLTPSRIRLLSCTRTFDCSKANDRLGYTPIVPLQEGLTRTIESYSDLRAE 1118
+F PYGMKVPQL PSRIRLLSC+R+F+CSKA DRLGY PIVPL+EGL RT+ESY LRAE
Sbjct: 301 IFGPYGMKVPQLIPSRIRLLSCSRSFNCSKAKDRLGYAPIVPLEEGLKRTLESYPHLRAE 360
Score = 161 bits (407), Expect(2) = 0.0
Identities = 81/157 (51%), Positives = 102/157 (64%)
Frame = +1
Query: 1210 TILVLFAIYFNFVASGYTIVTAVSKLLAVASILLFLHGKSPEKILGYTVEKVPESKFHLS 1389
T+LVL AIY+NF+AS TIVTA SKLL V S+ LF+HG PEK+ GYT+EK+P S F+LS
Sbjct: 393 TLLVLVAIYYNFIASQSTIVTAASKLLLVVSVFLFIHGNLPEKVFGYTIEKIPMSYFNLS 452
Query: 1390 KEMSHKVSLSMASSWNYVVNGLKSLCCGNDWPXXXXXXXXXXXXXXXXXXXXXXXFTIVL 1569
+E SH+ +LS+ASSWN VN LKSLC GNDW F I L
Sbjct: 453 EERSHQTALSVASSWNAAVNVLKSLCKGNDWSLFFQVVISLLILSFLGAISFQTLFIIGL 512
Query: 1570 PLVFVAFFVYEKKEEEIDGLVLRIISIGSKLKSDAEK 1680
P+ F AF++YEK E+ ID + L +S+G K+KSD K
Sbjct: 513 PVAFAAFYLYEKNEQAIDSMFLEALSLGCKMKSDITK 549
>ref|NP_180194.2| 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
[Arabidopsis thaliana]
gi|229890388|sp|Q67ZE1.2|HSDD2_ARATH RecName:
Full=3beta-hydroxysteroid-dehydrogenase/decarboxylase
isoform 2; Short=At3BETAHSD/D2; AltName:
Full=4alpha-carboxysterol-C3-dehydrogenase/C4-
decarboxylase isoform 1-2; AltName: Full=Reticulon-like
protein B19; Short=AtRTNLB19; AltName:
Full=Sterol-4-alpha-carboxylate 3-dehydrogenase 2,
decarboxylating gi|330252720|gb|AEC07814.1|
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2 [Arabidopsis thaliana]
Length = 564
Score = 578 bits (1489), Expect(2) = 0.0
Identities = 284/355 (80%), Positives = 310/355 (87%)
Frame = +3
Query: 51 ERWCVVTGGRGFAARHLVEMLLRYEMFSVRIADLGPSINLHPCEENGTLGEALRSGRAAY 230
ERWCVVTGGRGFAARHLVEML+RYEMF VRIADL P+I L P E NG L E LRSGR Y
Sbjct: 9 ERWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQY 68
Query: 231 VSADLRDKSQVSKACEGAEVVFHMAAPDSSINNHQLHQSVNVQGTANVIEACVESKVKRL 410
+SADLRDKSQV KA +GAEVVFHMAAPDSSINNHQL SVNVQGT NVI+ACV+ VKRL
Sbjct: 69 ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128
Query: 411 IYTSSASVVFDGVHGIYNGDESLPYPSKHNDSYSATKADGEALVIKANGVNGLLTCCIRP 590
IYTSS SVVFDGVHGI NG ES+ YP KHNDSYSATKA+GE L++KANG NGLLTCCIRP
Sbjct: 129 IYTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRP 188
Query: 591 SSIFGPGDKLLVPSLVSAARAGKSKFIIGDGNNMYDFTYVENVAHAHICAERALASDGAV 770
SSIFGPGD+LLVPSLV+AARAGKSKFIIGDGNN+YDFTYVENVAHAH+CAERALAS G V
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248
Query: 771 AQKAAGQAYFITNMEPIKFWEFMSLILGGLGYERPRIKVPVSVMMPIAHVVEAIYNVFAP 950
+ KAAGQAYFITNMEPIKFWEFMS +L GLGYERP IK+P +MMPIAH+VE Y V P
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGP 308
Query: 951 YGMKVPQLTPSRIRLLSCTRTFDCSKANDRLGYTPIVPLQEGLTRTIESYSDLRA 1115
YGM VPQLTPSR+RLLSC+RTFD +KA DRLGY P+VPLQEG+ RTI+S+S L A
Sbjct: 309 YGMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTA 363
Score = 159 bits (401), Expect(2) = 0.0
Identities = 81/153 (52%), Positives = 99/153 (64%)
Frame = +1
Query: 1213 ILVLFAIYFNFVASGYTIVTAVSKLLAVASILLFLHGKSPEKILGYTVEKVPESKFHLSK 1392
I +L +IY+NFVA+G T+VTA+SK L VAS+ LFLHG PEKI GYTVEK+P S+FHLSK
Sbjct: 400 IFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIFGYTVEKIPASQFHLSK 459
Query: 1393 EMSHKVSLSMASSWNYVVNGLKSLCCGNDWPXXXXXXXXXXXXXXXXXXXXXXXFTIVLP 1572
+ SH +SLS+ SSWN V L+SLC GNDW F I LP
Sbjct: 460 DSSHDLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLHSIFVIGLP 519
Query: 1573 LVFVAFFVYEKKEEEIDGLVLRIISIGSKLKSD 1671
+ F+AF VYEKKE+EID +V+ S K KSD
Sbjct: 520 IAFLAFLVYEKKEQEIDSIVVSFKSFACKHKSD 552
>ref|XP_002878951.1| 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2
[Arabidopsis lyrata subsp. lyrata]
gi|297324790|gb|EFH55210.1|
3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform
2 [Arabidopsis lyrata subsp. lyrata]
Length = 564
Score = 576 bits (1485), Expect(2) = 0.0
Identities = 284/355 (80%), Positives = 311/355 (87%)
Frame = +3
Query: 51 ERWCVVTGGRGFAARHLVEMLLRYEMFSVRIADLGPSINLHPCEENGTLGEALRSGRAAY 230
ERWCVVTGGRGFAARHLVEML+RYEMFSVRIADL P+I L EENG L E LRSGR Y
Sbjct: 9 ERWCVVTGGRGFAARHLVEMLVRYEMFSVRIADLAPAIMLDLHEENGVLDEGLRSGRVQY 68
Query: 231 VSADLRDKSQVSKACEGAEVVFHMAAPDSSINNHQLHQSVNVQGTANVIEACVESKVKRL 410
+SADLRDKSQV KA +GAEVVFHMAAPDSSINNHQL SVNVQGT NVI+ACV+ VKRL
Sbjct: 69 ISADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRL 128
Query: 411 IYTSSASVVFDGVHGIYNGDESLPYPSKHNDSYSATKADGEALVIKANGVNGLLTCCIRP 590
IYTSS SVVFDGVHGI NG ES+ YP KHNDSYSATKA+GE L++KANG GLLTCCIRP
Sbjct: 129 IYTSSPSVVFDGVHGILNGSESMAYPIKHNDSYSATKAEGEELIMKANGRKGLLTCCIRP 188
Query: 591 SSIFGPGDKLLVPSLVSAARAGKSKFIIGDGNNMYDFTYVENVAHAHICAERALASDGAV 770
SSIFGPGD+LLVPSLV+AARAGKSKFIIGDGNN+YDFTYVENVAHAH+CAERALAS G V
Sbjct: 189 SSIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDV 248
Query: 771 AQKAAGQAYFITNMEPIKFWEFMSLILGGLGYERPRIKVPVSVMMPIAHVVEAIYNVFAP 950
+ KAAGQAYFITNMEPIKFWEF+S +L GLGYERP IK+P VMMPIAH+VE Y + P
Sbjct: 249 STKAAGQAYFITNMEPIKFWEFVSQLLEGLGYERPSIKIPAFVMMPIAHLVELTYKLLGP 308
Query: 951 YGMKVPQLTPSRIRLLSCTRTFDCSKANDRLGYTPIVPLQEGLTRTIESYSDLRA 1115
YGMKVPQLTPSR+RLLSC+RTFD +KA DRLGY P+VPLQEG+ RTI+S+S L A
Sbjct: 309 YGMKVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSHLTA 363
Score = 156 bits (394), Expect(2) = 0.0
Identities = 79/153 (51%), Positives = 98/153 (64%)
Frame = +1
Query: 1213 ILVLFAIYFNFVASGYTIVTAVSKLLAVASILLFLHGKSPEKILGYTVEKVPESKFHLSK 1392
I +L ++Y+NFVA+G TIVTA+SK L VAS+ LFLHG PEKI GYTVEK+P S+FHLSK
Sbjct: 400 IFILISVYYNFVATGSTIVTALSKALLVASVFLFLHGILPEKIFGYTVEKIPASQFHLSK 459
Query: 1393 EMSHKVSLSMASSWNYVVNGLKSLCCGNDWPXXXXXXXXXXXXXXXXXXXXXXXFTIVLP 1572
+ S +SLS+ SSWN V L+SLC GNDW F I +P
Sbjct: 460 DSSEHLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLHNIFVIGIP 519
Query: 1573 LVFVAFFVYEKKEEEIDGLVLRIISIGSKLKSD 1671
+ F+AF VYEKKE+EID +V+ S K KSD
Sbjct: 520 IAFIAFIVYEKKEQEIDSIVVSFKSFACKRKSD 552