BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00016796
         (884 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ADD63989.1| nitric oxide synthase-associated protein I [Solan...   380   e-103
gb|ADK95138.1| nitric oxide-associated protein 1 [Nicotiana atte...   373   e-101
dbj|BAF93184.1| nitric oxide associated 1 [Nicotiana benthamiana]     373   e-101
ref|XP_002510962.1| Nitric-oxide synthase, putative [Ricinus com...   363   4e-98
ref|XP_002268388.1| PREDICTED: nitric oxide synthase 1 [Vitis vi...   358   1e-96

>gb|ADD63989.1| nitric oxide synthase-associated protein I [Solanum tuberosum]
          Length = 561

 Score =  380 bits (975), Expect = e-103
 Identities = 207/297 (69%), Positives = 226/297 (76%), Gaps = 8/297 (2%)
 Frame = +3

Query: 18  MAPKTLFLSPLY---PFLCSNS-----NHLLISNSRPLFIICKSTISHTNSPDPQSVRVS 173
           MAPK L LS L    PF   NS     N       +P  I CKST       +PQ++  S
Sbjct: 1   MAPKLLALSSLSSICPFPIPNSSPSSLNIYKFHTPKPTLIFCKST-------EPQTISES 53

Query: 174 ISTTVEPSGSGAAAPTRGDIFLERQKALEASAKELATADTXXXXXXXVSGYNKGSVIDYS 353
                EP G GAAAPTRGDI+L+RQ+ + AS+  LAT          +    K S +   
Sbjct: 54  -----EPEGYGAAAPTRGDIYLQRQQVVAASSSVLATTKKKKKKKDNIF---KISNLAPC 105

Query: 354 CYGCGAPLQTTETDAPGFVDTPTYELKKKHHQLRTVLCGRCQLLSHGHMITAVGGNGGYS 533
           CYGCGAPL T+E DAPG+VD  TY+LKKKHHQLR +LCGRC+LLSHGHMITAVGGNGGYS
Sbjct: 106 CYGCGAPLHTSEVDAPGYVDQETYDLKKKHHQLRKILCGRCRLLSHGHMITAVGGNGGYS 165

Query: 534 GGKQFVTAEELREKLSHLRHEKALIVKLVDIVDFNGSFLARVRDLAGANPIILVVTKVDL 713
           GGKQFVTAEELREKLSH+RHEKALIVKLVDIVDFNGSFLARVRDLAGANPIILV+TKVDL
Sbjct: 166 GGKQFVTAEELREKLSHVRHEKALIVKLVDIVDFNGSFLARVRDLAGANPIILVITKVDL 225

Query: 714 LPKGTDFNCVGDWVVEATMKKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKGRDVYI 884
           LPK TD NCVGDWVVEATMKKKLNVLSVHLTSSKSLVGI+GVVSEIQKEKKGRDVYI
Sbjct: 226 LPKDTDLNCVGDWVVEATMKKKLNVLSVHLTSSKSLVGISGVVSEIQKEKKGRDVYI 282


>gb|ADK95138.1| nitric oxide-associated protein 1 [Nicotiana attenuata]
          Length = 548

 Score =  373 bits (958), Expect = e-101
 Identities = 197/262 (75%), Positives = 214/262 (81%)
 Frame = +3

Query: 99  RPLFIICKSTISHTNSPDPQSVRVSISTTVEPSGSGAAAPTRGDIFLERQKALEASAKEL 278
           +P  I+CK+T S T         VS+S   EP G GAAAPTRGDI+L+RQ+A+ AS   +
Sbjct: 30  KPNLILCKTTESET---------VSVS---EPDGYGAAAPTRGDIYLQRQQAVAASTSVI 77

Query: 279 ATADTXXXXXXXVSGYNKGSVIDYSCYGCGAPLQTTETDAPGFVDTPTYELKKKHHQLRT 458
           AT          +    K S +   CYGCGAPL T+E DAPG+VD  TY+LKKKHHQLR 
Sbjct: 78  ATTKKKKKKKDKIF---KVSNLAPCCYGCGAPLHTSEVDAPGYVDQETYDLKKKHHQLRK 134

Query: 459 VLCGRCQLLSHGHMITAVGGNGGYSGGKQFVTAEELREKLSHLRHEKALIVKLVDIVDFN 638
           VLCGRC+LLSHGHMITAVGGNGGYSGGKQFVTAEELREKLSHLR+EKALIVKLVDIVDFN
Sbjct: 135 VLCGRCRLLSHGHMITAVGGNGGYSGGKQFVTAEELREKLSHLRNEKALIVKLVDIVDFN 194

Query: 639 GSFLARVRDLAGANPIILVVTKVDLLPKGTDFNCVGDWVVEATMKKKLNVLSVHLTSSKS 818
           GSFLARVRDLAGANPIILV+TKVDLLPK TD NCVGDWVVEATMKKKLNVLSVHLTSSKS
Sbjct: 195 GSFLARVRDLAGANPIILVITKVDLLPKETDLNCVGDWVVEATMKKKLNVLSVHLTSSKS 254

Query: 819 LVGIAGVVSEIQKEKKGRDVYI 884
           LVGIAGVVSEIQKEKKGRDVYI
Sbjct: 255 LVGIAGVVSEIQKEKKGRDVYI 276


>dbj|BAF93184.1| nitric oxide associated 1 [Nicotiana benthamiana]
          Length = 568

 Score =  373 bits (958), Expect = e-101
 Identities = 210/305 (68%), Positives = 225/305 (73%), Gaps = 16/305 (5%)
 Frame = +3

Query: 18  MAPKTLFLSPLY---PFLCSNSNHLLISNS-------------RPLFIICKSTISHTNSP 149
           MAPK L LS L    PF   N  H+   NS             +   I+CKST S T S 
Sbjct: 1   MAPKLLALSSLSSISPFRLPNY-HVSTPNSTLSKLNIFKFHTTKTNLILCKSTESQTVSE 59

Query: 150 DPQSVRVSISTTVEPSGSGAAAPTRGDIFLERQKALEASAKELATADTXXXXXXXVSGYN 329
                        EP G GAAAPTRGDI+L+RQ+A+ AS+  LAT          +    
Sbjct: 60  S------------EPDGYGAAAPTRGDIYLQRQQAVAASSTVLATIKKKKKKKDKIF--- 104

Query: 330 KGSVIDYSCYGCGAPLQTTETDAPGFVDTPTYELKKKHHQLRTVLCGRCQLLSHGHMITA 509
           K S +   CYGCGAPL T+E DAPG+V   TY+LKKKHHQLR VLCGRC+LLSHGHMITA
Sbjct: 105 KISNLAPCCYGCGAPLHTSEVDAPGYVHQETYDLKKKHHQLRKVLCGRCRLLSHGHMITA 164

Query: 510 VGGNGGYSGGKQFVTAEELREKLSHLRHEKALIVKLVDIVDFNGSFLARVRDLAGANPII 689
           VGGNGGYSGGKQFVTAEELREKLSHLRHEKALIVKLVDIVDFNGSFLARVRDLAGANPII
Sbjct: 165 VGGNGGYSGGKQFVTAEELREKLSHLRHEKALIVKLVDIVDFNGSFLARVRDLAGANPII 224

Query: 690 LVVTKVDLLPKGTDFNCVGDWVVEATMKKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKG 869
           LV+TKVDLLPK TD NCVGDWVVEATMKKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKG
Sbjct: 225 LVITKVDLLPKETDLNCVGDWVVEATMKKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKG 284

Query: 870 RDVYI 884
           RDVYI
Sbjct: 285 RDVYI 289


>ref|XP_002510962.1| Nitric-oxide synthase, putative [Ricinus communis]
           gi|223550077|gb|EEF51564.1| Nitric-oxide synthase,
           putative [Ricinus communis]
          Length = 567

 Score =  363 bits (931), Expect = 4e-98
 Identities = 195/294 (66%), Positives = 223/294 (75%), Gaps = 5/294 (1%)
 Frame = +3

Query: 18  MAPKTL--FLSPL---YPFLCSNSNHLLISNSRPLFIICKSTISHTNSPDPQSVRVSIST 182
           MAPKTL  F SP+   +     N+  L  ++ +   I CKST S         + +SI  
Sbjct: 1   MAPKTLVTFFSPVSLPHNLTHFNTKFLKFNSRKTTPIFCKSTQSQQKPVSDTQLPLSIPE 60

Query: 183 TVEPSGSGAAAPTRGDIFLERQKALEASAKELATADTXXXXXXXVSGYNKGSVIDYSCYG 362
           T   SG+GAAAPT G+ FLERQ+A +A+   +  A              K + +   CYG
Sbjct: 61  T---SGTGAAAPTPGEQFLERQQAFQAAKLVMKEAKKKRRREKVREKALKVNSMVVCCYG 117

Query: 363 CGAPLQTTETDAPGFVDTPTYELKKKHHQLRTVLCGRCQLLSHGHMITAVGGNGGYSGGK 542
           CGAPLQT + +APG+V   TYELKK+HHQLRTVLCGRC+LLSHGHMITAVGGNGGYSGGK
Sbjct: 118 CGAPLQTLDQEAPGYVQPDTYELKKRHHQLRTVLCGRCKLLSHGHMITAVGGNGGYSGGK 177

Query: 543 QFVTAEELREKLSHLRHEKALIVKLVDIVDFNGSFLARVRDLAGANPIILVVTKVDLLPK 722
           QFV+A+ELREKLSHLR+E+ L+VKLVDIVDFNGSFLAR+RDLAGANPIILVVTKVDLLPK
Sbjct: 178 QFVSADELREKLSHLRYERVLVVKLVDIVDFNGSFLARIRDLAGANPIILVVTKVDLLPK 237

Query: 723 GTDFNCVGDWVVEATMKKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKGRDVYI 884
            TDFNC+GDWVVEAT KKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKGRDVYI
Sbjct: 238 ETDFNCIGDWVVEATTKKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKGRDVYI 291


>ref|XP_002268388.1| PREDICTED: nitric oxide synthase 1 [Vitis vinifera]
           gi|296084222|emb|CBI24610.3| unnamed protein product
           [Vitis vinifera]
          Length = 564

 Score =  358 bits (918), Expect = 1e-96
 Identities = 199/294 (67%), Positives = 221/294 (75%), Gaps = 3/294 (1%)
 Frame = +3

Query: 12  LSMAPKT-LFLSPL-YPFLCSNSNHLLIS-NSRPLFIICKSTISHTNSPDPQSVRVSIST 182
           +++ P T +FLSPL  P+  SN      S  ++P  I C++      +P     R     
Sbjct: 1   MALKPLTSVFLSPLSLPYSPSNPTPKFSSFYTKPTPISCQTQAHQQAAPTSDPYRP---- 56

Query: 183 TVEPSGSGAAAPTRGDIFLERQKALEASAKELATADTXXXXXXXVSGYNKGSVIDYSCYG 362
             E  G GAAAPTRGD+FLE  +++ AS  E+             SG  K S    +CYG
Sbjct: 57  --ESDGLGAAAPTRGDLFLEHHQSVAAS--EVVFNANKKKKKVKFSGSWKASAAS-ACYG 111

Query: 363 CGAPLQTTETDAPGFVDTPTYELKKKHHQLRTVLCGRCQLLSHGHMITAVGGNGGYSGGK 542
           CGAPLQT ETDAPG+VD  TYELKKKH QLRTVLCGRC+LLSHG MITAVGGNGGYSGGK
Sbjct: 112 CGAPLQTLETDAPGYVDPETYELKKKHRQLRTVLCGRCRLLSHGQMITAVGGNGGYSGGK 171

Query: 543 QFVTAEELREKLSHLRHEKALIVKLVDIVDFNGSFLARVRDLAGANPIILVVTKVDLLPK 722
           QF++AEELREKLSHLRHEKALIVKLVDIVDFNGSFLA VRDLAGANPIILVVTKVDLLPK
Sbjct: 172 QFISAEELREKLSHLRHEKALIVKLVDIVDFNGSFLAHVRDLAGANPIILVVTKVDLLPK 231

Query: 723 GTDFNCVGDWVVEATMKKKLNVLSVHLTSSKSLVGIAGVVSEIQKEKKGRDVYI 884
            TD NCVGDWVVEATMKKKLNVLSVHLTSSKSLVGI+GV SEIQKEKKGR+VYI
Sbjct: 232 ETDLNCVGDWVVEATMKKKLNVLSVHLTSSKSLVGISGVASEIQKEKKGRNVYI 285