BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00016512
         (1233 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003633914.1| PREDICTED: spastin-like [Vitis vinifera] gi|...   535   e-150
gb|AAL25088.1|AF426837_1 Tobacco mosaic virus helicase domain-bi...   531   e-148
ref|XP_003623772.1| Spastin [Medicago truncatula] gi|355498787|g...   520   e-145
ref|XP_003552348.1| PREDICTED: spastin-like [Glycine max]             518   e-144
ref|XP_002511743.1| Spastin, putative [Ricinus communis] gi|2235...   509   e-142

>ref|XP_003633914.1| PREDICTED: spastin-like [Vitis vinifera] gi|296089089|emb|CBI38792.3|
            unnamed protein product [Vitis vinifera]
          Length = 491

 Score =  535 bits (1379), Expect = e-150
 Identities = 289/413 (69%), Positives = 320/413 (77%), Gaps = 2/413 (0%)
 Frame = +1

Query: 1    VSNERAAYKFKGYLDLATAEIDKAVRAEEWGLMNDAVLHYQNAQRILIEAXXXXXXXXXX 180
            VSN+R AYK KGY DLAT EI KAVRAEEWGL++DA++HY+NAQRILIEA          
Sbjct: 44   VSNQRIAYKLKGYYDLATEEIAKAVRAEEWGLVDDAIVHYKNAQRILIEASSTSTPSFIS 103

Query: 181  XXEQEKVKSYREKISKWQGQVSDRLLVLRRRTGGKSVNKAQSDPTKXXXXXXXXXXXXXX 360
              EQEKVKSYR+KISKWQGQV++RL +L RR GG S NK     TK              
Sbjct: 104  SSEQEKVKSYRQKISKWQGQVAERLEILIRRAGGTSTNKNTLADTKP------------- 150

Query: 361  XXXXXXXXXXXXXAQTGAVIQTASSPRKVASKVSSGPNKYGSGIKSETKKVVAPKPAQNN 540
                                 T S+ R    + S   N+    ++S++ KVV+ KP Q +
Sbjct: 151  ----------------ATTSSTKSNARTDVLQKSPLTNRRSPIMRSQSDKVVSSKPVQES 194

Query: 541  A--YDEKLVEMIESVIVDRSPSVKWEDIAGLEKAKQTLLEMVILPTKRKDLFTGLRRPSR 714
               YD KLVEMI +VIVDRSPSVKW+D+AGLEKAKQ LLEMVILPTKRKDLFTGLRRP+R
Sbjct: 195  GHGYDAKLVEMINTVIVDRSPSVKWDDVAGLEKAKQALLEMVILPTKRKDLFTGLRRPAR 254

Query: 715  GLLLFGPPGTGKTMLAKAVASESEATFFNVSASSLTSKWVGEGEKLVRTLFMVAISRKPS 894
            GLLLFGPPG GKTMLAKAVASES ATFFNVSASSLTSKWVGEGEKLVRTLFMVAISR+PS
Sbjct: 255  GLLLFGPPGNGKTMLAKAVASESAATFFNVSASSLTSKWVGEGEKLVRTLFMVAISRQPS 314

Query: 895  VIFIDEIDSIMSTRTSDENEASRRLKSEFLVQFDGVTSNSDDLVIVIGATNKPQELDDAV 1074
            VIF+DEIDSIMSTR ++ENEASRRLKSEFLVQFDGVTSN DDLVIVIGATNKPQELDDAV
Sbjct: 315  VIFMDEIDSIMSTRMTNENEASRRLKSEFLVQFDGVTSNPDDLVIVIGATNKPQELDDAV 374

Query: 1075 LRRLVKRIYIPLPDANVRRILFKHKLKGQAFSLPSGDLERLVKDTEGYSGSDL 1233
            LRRLVKRIY+PLPD NVRR+L KHKLKGQAFSLP GDLERLV++TEGYSGSDL
Sbjct: 375  LRRLVKRIYVPLPDENVRRLLLKHKLKGQAFSLPGGDLERLVQETEGYSGSDL 427


>gb|AAL25088.1|AF426837_1 Tobacco mosaic virus helicase domain-binding protein [Nicotiana
            tabacum]
          Length = 537

 Score =  531 bits (1367), Expect = e-148
 Identities = 295/423 (69%), Positives = 322/423 (76%), Gaps = 14/423 (3%)
 Frame = +1

Query: 7    NERAAYKFKGYLDLATAEIDKAVRAEEWGLMNDAVLHYQNAQRILIEAXXXXXXXXXXXX 186
            NERAAYK KGY DLA  EIDKAVRAEEWGL +DA+ HYQNAQ+IL E             
Sbjct: 80   NERAAYKLKGYFDLAKEEIDKAVRAEEWGLADDAISHYQNAQKILAEGISTPVPSYITSS 139

Query: 187  EQEKVKSYREKISKWQGQVSDRLLVLRRRTGGKSVNKAQSDPTKXXXXXXXXXXXXXXXX 366
            EQEKVKSYR+K++KW+ QVS+RL  L RR GG S  K  +  T                 
Sbjct: 140  EQEKVKSYRQKLTKWKSQVSERLQTLSRRAGGTSAVKISAPQT----------------- 182

Query: 367  XXXXXXXXXXXAQTGAVIQTASSPRKVASKV--SSG--------PN--KYGSGIKSETKK 510
                        Q  AV Q++SS RK  S+   SSG        PN  K  S  +     
Sbjct: 183  ------------QRLAVSQSSSSARKGESRTAPSSGRGSSVMRVPNSGKDSSVARVPINS 230

Query: 511  VVAPKPAQN--NAYDEKLVEMIESVIVDRSPSVKWEDIAGLEKAKQTLLEMVILPTKRKD 684
            + + KP+Q   N YD KLV+MI SVIVDRSPSVKWEDIAGLEKAKQ LLEMVILPTKRKD
Sbjct: 231  ISSHKPSQESANGYDPKLVDMINSVIVDRSPSVKWEDIAGLEKAKQALLEMVILPTKRKD 290

Query: 685  LFTGLRRPSRGLLLFGPPGTGKTMLAKAVASESEATFFNVSASSLTSKWVGEGEKLVRTL 864
            LFTGLRRP+RGLLLFGPPGTGKTMLAKAVASESEATFFNVSASSLTSKWVGEGEKLV+TL
Sbjct: 291  LFTGLRRPARGLLLFGPPGTGKTMLAKAVASESEATFFNVSASSLTSKWVGEGEKLVKTL 350

Query: 865  FMVAISRKPSVIFIDEIDSIMSTRTSDENEASRRLKSEFLVQFDGVTSNSDDLVIVIGAT 1044
            FMVAISRKPSVIF+DEIDS+MSTRT++ENEASRRLKSEFLVQFDGVTSNSDDLVIVIGAT
Sbjct: 351  FMVAISRKPSVIFMDEIDSVMSTRTTNENEASRRLKSEFLVQFDGVTSNSDDLVIVIGAT 410

Query: 1045 NKPQELDDAVLRRLVKRIYIPLPDANVRRILFKHKLKGQAFSLPSGDLERLVKDTEGYSG 1224
            NKPQELDDAVLRRLVKRIYIPLPDANVRR L KH+LKG+AFSLP GDL+RLV+DTEGYSG
Sbjct: 411  NKPQELDDAVLRRLVKRIYIPLPDANVRRQLLKHRLKGKAFSLPGGDLDRLVRDTEGYSG 470

Query: 1225 SDL 1233
            SDL
Sbjct: 471  SDL 473


>ref|XP_003623772.1| Spastin [Medicago truncatula] gi|355498787|gb|AES79990.1| Spastin
            [Medicago truncatula]
          Length = 486

 Score =  520 bits (1338), Expect = e-145
 Identities = 277/414 (66%), Positives = 321/414 (77%), Gaps = 3/414 (0%)
 Frame = +1

Query: 1    VSNERAAYKFKGYLDLATAEIDKAVRAEEWGLMNDAVLHYQNAQRILIEAXXXXXXXXXX 180
            +SNER AYK KGY DLAT EIDKAVRAEEWGL++DA+LHY+NA RIL+EA          
Sbjct: 46   ISNERVAYKLKGYYDLATQEIDKAVRAEEWGLIDDAILHYRNAHRILLEANSTPVPSFIT 105

Query: 181  XXEQEKVKSYREKISKWQGQVSDRLLVLRRRTGGKSVNKAQSDPTKXXXXXXXXXXXXXX 360
              E++KV+SYR+KISKWQGQVS+RL  L RR G    N++ S+                 
Sbjct: 106  PSEKQKVQSYRQKISKWQGQVSERLQALSRRAGSSFANQSTSN----------------- 148

Query: 361  XXXXXXXXXXXXXAQTGAV-IQTASSPRKVASKVSSGPNKYGSGIKSETKKVVAPKPAQN 537
                         AQT AV  + +++ +KV  K    P +     K E  KV +PKP+Q 
Sbjct: 149  ------------RAQTAAVPTKLSNTTKKVLQK---NPQR-----KDEVNKVQSPKPSQT 188

Query: 538  NA--YDEKLVEMIESVIVDRSPSVKWEDIAGLEKAKQTLLEMVILPTKRKDLFTGLRRPS 711
            +   YD KLVEMI + IVDRSPSV+W+D+ GLEKAKQ L+EMVILPTKR+DLFTGLRRP+
Sbjct: 189  SGENYDTKLVEMINTAIVDRSPSVRWDDVGGLEKAKQALMEMVILPTKRRDLFTGLRRPA 248

Query: 712  RGLLLFGPPGTGKTMLAKAVASESEATFFNVSASSLTSKWVGEGEKLVRTLFMVAISRKP 891
            RGLLLFGPPG GKTMLAKAVASESEATFFNV+A+SLTSKWVGE EKLVRTLFMVA+SR+P
Sbjct: 249  RGLLLFGPPGNGKTMLAKAVASESEATFFNVTAASLTSKWVGEAEKLVRTLFMVAVSRQP 308

Query: 892  SVIFIDEIDSIMSTRTSDENEASRRLKSEFLVQFDGVTSNSDDLVIVIGATNKPQELDDA 1071
            SVIFIDEIDSIMSTRT++ENEASRRLKSEFL+QFDGVTSN DD+VIVIGATNKPQELDDA
Sbjct: 309  SVIFIDEIDSIMSTRTTNENEASRRLKSEFLIQFDGVTSNPDDIVIVIGATNKPQELDDA 368

Query: 1072 VLRRLVKRIYIPLPDANVRRILFKHKLKGQAFSLPSGDLERLVKDTEGYSGSDL 1233
            VLRRLVKRIY+PLP+ NVR++L KHKLKGQAFSLPS DLE LV++TEGYSGSDL
Sbjct: 369  VLRRLVKRIYVPLPNENVRKLLLKHKLKGQAFSLPSRDLEMLVRETEGYSGSDL 422


>ref|XP_003552348.1| PREDICTED: spastin-like [Glycine max]
          Length = 491

 Score =  518 bits (1333), Expect = e-144
 Identities = 281/413 (68%), Positives = 317/413 (76%), Gaps = 2/413 (0%)
 Frame = +1

Query: 1    VSNERAAYKFKGYLDLATAEIDKAVRAEEWGLMNDAVLHYQNAQRILIEAXXXXXXXXXX 180
            VSNER AYK KGY DLAT EI K VRAEEWGL++DA+LHY+NA  IL+EA          
Sbjct: 51   VSNERVAYKLKGYFDLATQEIAKGVRAEEWGLIDDALLHYRNAHSILLEANSTPVPSYIT 110

Query: 181  XXEQEKVKSYREKISKWQGQVSDRLLVLRRRTGGKSVNKAQSDPTKXXXXXXXXXXXXXX 360
              EQ+KV+SYREKISKWQ QVS+RL  L RR G  S N++ S                  
Sbjct: 111  SSEQQKVQSYREKISKWQSQVSERLQTLARRAGSSSANQSTSK----------------- 153

Query: 361  XXXXXXXXXXXXXAQTGAVIQTASSPRKVASKVSSGPNKYGSGIKSETKKVVAPKPAQNN 540
                         AQT AV    SS RK  + +   P + G     +  KV +PK +Q +
Sbjct: 154  ------------LAQTVAVPIKPSSTRK--NVLQKPPQRTG-----QVNKVGSPKSSQGS 194

Query: 541  A--YDEKLVEMIESVIVDRSPSVKWEDIAGLEKAKQTLLEMVILPTKRKDLFTGLRRPSR 714
               YD+KLVEMI + IVDRSPSV+WED+AGLEKAKQ L+EMVILPTKR+DLFTGLRRP+R
Sbjct: 195  GVNYDDKLVEMINTAIVDRSPSVRWEDVAGLEKAKQALMEMVILPTKRRDLFTGLRRPAR 254

Query: 715  GLLLFGPPGTGKTMLAKAVASESEATFFNVSASSLTSKWVGEGEKLVRTLFMVAISRKPS 894
            GLLLFGPPG GKTMLAKAVASES+ATFFNV+A+SLTSKWVGEGEKLVRTLFMVAISR+PS
Sbjct: 255  GLLLFGPPGNGKTMLAKAVASESQATFFNVTAASLTSKWVGEGEKLVRTLFMVAISRQPS 314

Query: 895  VIFIDEIDSIMSTRTSDENEASRRLKSEFLVQFDGVTSNSDDLVIVIGATNKPQELDDAV 1074
            VIFIDEIDSIMSTR ++EN+ASRRLKSEFL+QFDGVTSN DD+VIVIGATNKPQELDDAV
Sbjct: 315  VIFIDEIDSIMSTRLANENDASRRLKSEFLIQFDGVTSNPDDIVIVIGATNKPQELDDAV 374

Query: 1075 LRRLVKRIYIPLPDANVRRILFKHKLKGQAFSLPSGDLERLVKDTEGYSGSDL 1233
            LRRLVKRIYIPLPD NVR++L KHKLKGQAFSLPS DLERLVK+TEGYSGSDL
Sbjct: 375  LRRLVKRIYIPLPDENVRKLLLKHKLKGQAFSLPSRDLERLVKETEGYSGSDL 427


>ref|XP_002511743.1| Spastin, putative [Ricinus communis] gi|223548923|gb|EEF50412.1|
            Spastin, putative [Ricinus communis]
          Length = 518

 Score =  509 bits (1312), Expect = e-142
 Identities = 275/423 (65%), Positives = 311/423 (73%), Gaps = 12/423 (2%)
 Frame = +1

Query: 1    VSNERAAYKFKGYLDLATAEIDKAVRAEEWGLMNDAVLHYQNAQRILIEAXXXXXXXXXX 180
            V+NER AYK KGY DLA  EIDKAVRAEEWGL +DA++HY+NAQRI IE           
Sbjct: 40   VTNERVAYKLKGYFDLAKEEIDKAVRAEEWGLPDDAIVHYKNAQRIFIEGNSTSVPSYIS 99

Query: 181  XXEQEKVKSYREKISKWQGQVSDRLLVLRRRTGGKSVNK--------AQSDPTKXXXXXX 336
              EQEKVKSYR+KISKWQG +S+RL  L RR  G S NK        A    +K      
Sbjct: 100  FSEQEKVKSYRQKISKWQGHISERLQALNRRAAGTSSNKNTLTHAHTAAVSSSKSNFRQD 159

Query: 337  XXXXXXXXXXXXXXXXXXXXXAQTGAVIQTA--SSPRKVASKVSSGPNKYGSGIKSETKK 510
                                 A    + Q    +SPR   +   +         +++   
Sbjct: 160  LSQKSPCSTRNTPVVRNQPNKAAKSKIAQNMPQNSPRSTGNTAVT---------RNQPDT 210

Query: 511  VVAPKPAQN--NAYDEKLVEMIESVIVDRSPSVKWEDIAGLEKAKQTLLEMVILPTKRKD 684
               PK AQ   N Y+ KLVEMI + IVDRSPSVKW+D+AGLEKAKQ+L+EMVILPTKR+D
Sbjct: 211  AAKPKSAQESGNGYEAKLVEMINTAIVDRSPSVKWDDVAGLEKAKQSLMEMVILPTKRRD 270

Query: 685  LFTGLRRPSRGLLLFGPPGTGKTMLAKAVASESEATFFNVSASSLTSKWVGEGEKLVRTL 864
            LFTGLRRP+RGLLLFGPPG GKTMLAKAVASESEATFFNVSASSLTSKWVGEGEKLVRTL
Sbjct: 271  LFTGLRRPARGLLLFGPPGNGKTMLAKAVASESEATFFNVSASSLTSKWVGEGEKLVRTL 330

Query: 865  FMVAISRKPSVIFIDEIDSIMSTRTSDENEASRRLKSEFLVQFDGVTSNSDDLVIVIGAT 1044
            FMVAISR+PSVIF+DEIDSIMSTR ++EN+ASRRLKSEFL+QFDGVTSN +DLVIVIGAT
Sbjct: 331  FMVAISRQPSVIFLDEIDSIMSTRLTNENDASRRLKSEFLIQFDGVTSNPNDLVIVIGAT 390

Query: 1045 NKPQELDDAVLRRLVKRIYIPLPDANVRRILFKHKLKGQAFSLPSGDLERLVKDTEGYSG 1224
            NKPQELDDAVLRRLVKRIY+PLPD N+R +LFKHKLKGQAFSL  GDLERLV++TEGYSG
Sbjct: 391  NKPQELDDAVLRRLVKRIYVPLPDKNIRLLLFKHKLKGQAFSLSDGDLERLVRETEGYSG 450

Query: 1225 SDL 1233
            SDL
Sbjct: 451  SDL 453