BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015812
         (571 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002528228.1| ubiquitin-protein ligase, putative [Ricinus ...   192   3e-47
ref|XP_003546191.1| PREDICTED: U-box domain-containing protein 4...   190   1e-46
ref|XP_003594249.1| U-box domain-containing protein [Medicago tr...   189   2e-46
ref|XP_003534872.1| PREDICTED: U-box domain-containing protein 2...   189   3e-46
ref|XP_002323140.1| predicted protein [Populus trichocarpa] gi|2...   188   5e-46

>ref|XP_002528228.1| ubiquitin-protein ligase, putative [Ricinus communis]
           gi|223532345|gb|EEF34143.1| ubiquitin-protein ligase,
           putative [Ricinus communis]
          Length = 467

 Score =  192 bits (488), Expect = 3e-47
 Identities = 104/151 (68%), Positives = 123/151 (81%)
 Frame = -3

Query: 566 RGKKDPITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQGGEMAEKAMVVLCTLAGVESG 387
           RGKKD +TTLYK+C I +NKE+AVS GAVK LV MV +QG  MAEKAMVVL +LA +E G
Sbjct: 319 RGKKDALTTLYKLCSIKQNKERAVSAGAVKPLVGMVAEQGTGMAEKAMVVLSSLAAIEEG 378

Query: 386 KAKIVEEGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVALS 207
           +  IVEEGGI+ALVE I E+GS+KGKEFAV+TLLQLC+++V+NRGLLVREG IPPLVALS
Sbjct: 379 REAIVEEGGIAALVEAI-EDGSVKGKEFAVLTLLQLCADSVRNRGLLVREGGIPPLVALS 437

Query: 206 QSGTXXXXXXXKILIKYLRETRQEEASSSSP 114
           Q+GT       + L+ YLRE RQ EASSSSP
Sbjct: 438 QTGTVRAKHKAETLLGYLREPRQ-EASSSSP 467



 Score = 60.1 bits (144), Expect = 2e-07
 Identities = 43/119 (36%), Positives = 65/119 (54%), Gaps = 1/119 (0%)
 Frame = -3

Query: 548 ITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQGGEMAEKAMVVLCTLAGVESGKAKIVE 369
           +T L  + L +ENK    + GA+K LV ++    G   + A   L +LA VE  K+ I  
Sbjct: 243 VTALLNLSLHEENKGLITNNGAIKSLVYVLKTGTGTSKQNAACALLSLALVEENKSSIGA 302

Query: 368 EGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVAL-SQSGT 195
            G I  LV ++   GS +GK+ A+ TL +LCS   +N+   V  GA+ PLV + ++ GT
Sbjct: 303 CGAIPPLVSLL-ISGSSRGKKDALTTLYKLCSIK-QNKERAVSAGAVKPLVGMVAEQGT 359


>ref|XP_003546191.1| PREDICTED: U-box domain-containing protein 4-like [Glycine max]
          Length = 457

 Score =  190 bits (483), Expect = 1e-46
 Identities = 98/150 (65%), Positives = 123/150 (82%)
 Frame = -3

Query: 566 RGKKDPITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQGGEMAEKAMVVLCTLAGVESG 387
           RGKKD +TTLYK+C + +NKE+ VS GAVK LV++V +QG  MAEKAMVVL +LAG++ G
Sbjct: 308 RGKKDALTTLYKLCSVRQNKERTVSAGAVKPLVELVAEQGSGMAEKAMVVLNSLAGIQEG 367

Query: 386 KAKIVEEGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVALS 207
           K  IVEEGGI+ALVE I E+GS+KGKEFAV+TLLQLC ++V+NRG LVREG IPPLVALS
Sbjct: 368 KNAIVEEGGIAALVEAI-EDGSVKGKEFAVLTLLQLCVDSVRNRGFLVREGGIPPLVALS 426

Query: 206 QSGTXXXXXXXKILIKYLRETRQEEASSSS 117
           Q+G+       + L++YLRE+RQE AS+SS
Sbjct: 427 QTGSVRAKHKAETLLRYLRESRQEAASTSS 456



 Score = 57.8 bits (138), Expect = 1e-06
 Identities = 41/113 (36%), Positives = 60/113 (53%)
 Frame = -3

Query: 548 ITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQGGEMAEKAMVVLCTLAGVESGKAKIVE 369
           +T L  + L ++NK    + GAVK LV ++        + A   L +LA VE  K+ I  
Sbjct: 232 VTALLNLSLHEDNKMLITNAGAVKSLVYVLKTGTETSKQNAACALLSLALVEENKSSIGA 291

Query: 368 EGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVAL 210
            G I  LV ++   GS +GK+ A+ TL +LCS   +N+   V  GA+ PLV L
Sbjct: 292 SGAIPPLVSLL-LNGSSRGKKDALTTLYKLCSVR-QNKERTVSAGAVKPLVEL 342


>ref|XP_003594249.1| U-box domain-containing protein [Medicago truncatula]
           gi|355483297|gb|AES64500.1| U-box domain-containing
           protein [Medicago truncatula]
          Length = 460

 Score =  189 bits (481), Expect = 2e-46
 Identities = 98/150 (65%), Positives = 122/150 (81%)
 Frame = -3

Query: 566 RGKKDPITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQGGEMAEKAMVVLCTLAGVESG 387
           RGKKD +TTLYK+C + +NKE+AVS G VK LV++V +QG  M EKAMVVL +LAG + G
Sbjct: 312 RGKKDALTTLYKLCSVKQNKERAVSAGVVKPLVELVAEQGNGMMEKAMVVLNSLAGFDEG 371

Query: 386 KAKIVEEGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVALS 207
           K  IVEEGGI+ALVE I E+GS+KGKEFAV+TLLQLC+E+V NRGLLVREG IPPLVALS
Sbjct: 372 KEAIVEEGGIAALVEAI-EDGSVKGKEFAVLTLLQLCAESVTNRGLLVREGGIPPLVALS 430

Query: 206 QSGTXXXXXXXKILIKYLRETRQEEASSSS 117
           Q+GT       + L++YLRE+RQE ++S+S
Sbjct: 431 QNGTPRAKHKAETLLRYLRESRQEASTSTS 460



 Score = 56.2 bits (134), Expect = 4e-06
 Identities = 39/113 (34%), Positives = 60/113 (53%)
 Frame = -3

Query: 548 ITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQGGEMAEKAMVVLCTLAGVESGKAKIVE 369
           +T L  + L ++NK+   + GAVK L+ ++        + A   L +LA VE  K+ I  
Sbjct: 236 VTALLNLSLHEDNKKLIFNAGAVKSLIYVLKTGTETSKQNAACALLSLALVEENKSSIGA 295

Query: 368 EGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVAL 210
            G I  LV ++   GS +GK+ A+ TL +LCS   +N+   V  G + PLV L
Sbjct: 296 SGAIPPLVSLL-LNGSNRGKKDALTTLYKLCSVK-QNKERAVSAGVVKPLVEL 346


>ref|XP_003534872.1| PREDICTED: U-box domain-containing protein 2-like [Glycine max]
          Length = 458

 Score =  189 bits (480), Expect = 3e-46
 Identities = 99/150 (66%), Positives = 122/150 (81%)
 Frame = -3

Query: 566 RGKKDPITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQGGEMAEKAMVVLCTLAGVESG 387
           RGKKD +TTLYK+C + +NKE+AVS GAVK LV++V +QG  MAEKAMVVL +LAG++ G
Sbjct: 309 RGKKDALTTLYKLCSVRQNKERAVSAGAVKPLVELVAEQGNGMAEKAMVVLNSLAGIQEG 368

Query: 386 KAKIVEEGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVALS 207
           K  IVEEGGI+ALVE I E+GS+KGKEFAV+TLLQLC ++V NRG LVREG IPPLVALS
Sbjct: 369 KDAIVEEGGIAALVEAI-EDGSVKGKEFAVLTLLQLCVDSVINRGFLVREGGIPPLVALS 427

Query: 206 QSGTXXXXXXXKILIKYLRETRQEEASSSS 117
           Q+G+       + L++YLRE RQE AS+SS
Sbjct: 428 QTGSARAKHKAETLLRYLREPRQEAASTSS 457



 Score = 57.0 bits (136), Expect = 2e-06
 Identities = 40/113 (35%), Positives = 59/113 (52%)
 Frame = -3

Query: 548 ITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQGGEMAEKAMVVLCTLAGVESGKAKIVE 369
           +T L  + L ++NK    + GAVK L+ ++        + A   L +LA VE  K  I  
Sbjct: 233 VTALLNLSLHEDNKMLITNAGAVKSLIYVLKTGTETSKQNAACALLSLALVEENKGSIGA 292

Query: 368 EGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVAL 210
            G I  LV ++   GS +GK+ A+ TL +LCS   +N+   V  GA+ PLV L
Sbjct: 293 SGAIPPLVSLL-LNGSSRGKKDALTTLYKLCSVR-QNKERAVSAGAVKPLVEL 343


>ref|XP_002323140.1| predicted protein [Populus trichocarpa] gi|222867770|gb|EEF04901.1|
           predicted protein [Populus trichocarpa]
          Length = 474

 Score =  188 bits (478), Expect = 5e-46
 Identities = 104/152 (68%), Positives = 123/152 (80%), Gaps = 1/152 (0%)
 Frame = -3

Query: 566 RGKKDPITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQG-GEMAEKAMVVLCTLAGVES 390
           RGKKD +TTLYKIC I +NKE+AV  GAVK LV MV + G G MAEKAMVVL +LA ++ 
Sbjct: 324 RGKKDALTTLYKICSIKQNKERAVIAGAVKPLVGMVVEAGAGMMAEKAMVVLSSLAAIQE 383

Query: 389 GKAKIVEEGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVAL 210
           G+  IVEEGGI+ALVE IE+ GS+KGKEFAV+TLLQLCS++V+NRGLLVREGAIPPLVAL
Sbjct: 384 GRDAIVEEGGIAALVEAIED-GSVKGKEFAVVTLLQLCSDSVRNRGLLVREGAIPPLVAL 442

Query: 209 SQSGTXXXXXXXKILIKYLRETRQEEASSSSP 114
           SQ+G+       + L+ YLRE RQE ASSSSP
Sbjct: 443 SQNGSIRSKNKAERLLGYLREPRQEAASSSSP 474



 Score = 57.8 bits (138), Expect = 1e-06
 Identities = 39/113 (34%), Positives = 61/113 (53%)
 Frame = -3

Query: 548 ITTLYKICLIDENKEKAVSGGAVKLLVDMVGDQGGEMAEKAMVVLCTLAGVESGKAKIVE 369
           +T L  + L +ENK+K    GA+K LV ++        + A   L +LA +E  K+ I  
Sbjct: 248 VTALLNLSLYEENKKKITKSGAIKSLVYVLKTGTENAKQNAACALLSLALIEVNKSSIGA 307

Query: 368 EGGISALVEVIEEEGSIKGKEFAVMTLLQLCSENVKNRGLLVREGAIPPLVAL 210
            G I  LV ++   GS +GK+ A+ TL ++CS   +N+   V  GA+ PLV +
Sbjct: 308 CGAIPPLVSLL-INGSNRGKKDALTTLYKICSIK-QNKERAVIAGAVKPLVGM 358