BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Bupleurum21_contig00015573
         (1754 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAC83147.1| 1-aminocyclopropane-1-carboxylate synthase [Nicot...   728   0.0  
emb|CAA06288.1| 1-cyclopropane-1-carboxylate synthase [Nicotiana...   728   0.0  
emb|CAA67118.1| ACC synthase [Nicotiana tabacum]                      728   0.0  
gb|AAR99392.1| ACC synthase ACS3a [Nicotiana attenuata]               721   0.0  
gb|AAF97614.1|U18056_1 1-amino-cyclopropane-1-carboxylate syntha...   721   0.0  

>gb|AAC83147.1| 1-aminocyclopropane-1-carboxylate synthase [Nicotiana glutinosa]
          Length = 484

 Score =  728 bits (1879), Expect = 0.0
 Identities = 345/464 (74%), Positives = 405/464 (87%), Gaps = 2/464 (0%)
 Frame = -2

Query: 1636 KNLLSRVATNDGHGENSPYFDGWKAYDSDPFHSDENPQGVIQMGLAENQLCFDLIQEWIL 1457
            + LLS++ATNDGHGENS YFDGWKAY++DPFH   NP GVIQMGLAENQLCFDLIQEW++
Sbjct: 11   QQLLSKIATNDGHGENSAYFDGWKAYENDPFHLINNPNGVIQMGLAENQLCFDLIQEWVV 70

Query: 1456 KNPKASICTSEGVNNFKDAAIFQDYHGLPQFREAIAKFMAKVRGNTVTFDPDRVVMSGGA 1277
             NPKASICT EG  NFKD AIFQDYHGLP+FR+A+A+FM  VRG+ VTFDP+R+VMSGGA
Sbjct: 71   NNPKASICTIEGAENFKDIAIFQDYHGLPEFRQAVARFMENVRGDRVTFDPNRIVMSGGA 130

Query: 1276 TGAHETMAFCLADPGDAFLVPTPYYPGFDRDLRWRTGVKLLPVVCESSNNFMITREALDA 1097
            TGAHET+AFCLA+PGDAFLVPTPYYPGFDRDLRWRTGV+L PVVC+SSN+F +T++AL+ 
Sbjct: 131  TGAHETLAFCLANPGDAFLVPTPYYPGFDRDLRWRTGVQLFPVVCDSSNDFKVTKKALET 190

Query: 1096 AYQKAQDSNIKVKGLLITNPSNPLGTILDRDTLQSLMDFINEKNIHLICDEIYAATVFTK 917
            AY+KAQ+SNI +KGLLI NPSNPLGT+LD DTL+ ++ FIN KNIHLICDEIYAATVF +
Sbjct: 191  AYEKAQESNINIKGLLINNPSNPLGTLLDSDTLRDIVKFINSKNIHLICDEIYAATVFDQ 250

Query: 916  PMYVSIAEILEDDKKCNRDLIHLVYSLSKDMGFPGFRIGIIYSYNDKVVNCARKMSSFGL 737
            P ++S++EI+E+   CN+DLIH+VYSLSKDMGFPGFR+GI+YSYND VVN ARKMSSFGL
Sbjct: 251  PKFISVSEIVEEHVGCNKDLIHIVYSLSKDMGFPGFRVGIVYSYNDTVVNIARKMSSFGL 310

Query: 736  VSTQTQELIANMLSDENFVDKYTSESRKRLAARHGVFTRGLMQVRVGNLQSNAGLFFWMD 557
            VSTQTQ LIA+MLSDE FV+K+ +ES +RL  RHG+FT+GL QV +  L+SNAGLFFWMD
Sbjct: 311  VSTQTQHLIASMLSDEIFVEKFIAESSERLGKRHGMFTKGLAQVGISTLKSNAGLFFWMD 370

Query: 556  LRRLLKEPTIAAEMELWREIINEVKINVSPGSSFHCSEPGWFRVCYANMDDETMRIALRR 377
            LRRLLKEPT  +E+ELWR IINEVK+NVSPG SFHCSEPGWFRVC+ANMDDETMRIALRR
Sbjct: 371  LRRLLKEPTFDSELELWRIIINEVKLNVSPGCSFHCSEPGWFRVCFANMDDETMRIALRR 430

Query: 376  IKKFVLQAD--EREAAAKKQMLNKRNLQLSLSFRRWDDTIMNTP 251
            I+ FVLQ      +AA KKQ  ++  LQ+SLSFRR DD  MN+P
Sbjct: 431  IRNFVLQTKGLNNKAAVKKQ-CSRSKLQISLSFRRMDD-FMNSP 472


>emb|CAA06288.1| 1-cyclopropane-1-carboxylate synthase [Nicotiana tabacum]
            gi|6688886|emb|CAB65314.1|
            1-aminocyclopropane-1-carboxylate synthase [Nicotiana
            tabacum] gi|159517167|gb|ABW97849.1| ACS2 [Nicotiana
            tabacum]
          Length = 484

 Score =  728 bits (1879), Expect = 0.0
 Identities = 343/464 (73%), Positives = 407/464 (87%), Gaps = 2/464 (0%)
 Frame = -2

Query: 1636 KNLLSRVATNDGHGENSPYFDGWKAYDSDPFHSDENPQGVIQMGLAENQLCFDLIQEWIL 1457
            + LLS++ATNDGHGENS YFDGWKAY++DPFH   NP GVIQMGLAENQLCFDLIQEW++
Sbjct: 11   QQLLSKIATNDGHGENSAYFDGWKAYENDPFHLSNNPNGVIQMGLAENQLCFDLIQEWVV 70

Query: 1456 KNPKASICTSEGVNNFKDAAIFQDYHGLPQFREAIAKFMAKVRGNTVTFDPDRVVMSGGA 1277
             NPKASICT+EG  +FKD AIFQDYHGLP+FR+A+A+FM KVRG+ VTFDP+R+VMSGGA
Sbjct: 71   NNPKASICTTEGAEDFKDIAIFQDYHGLPEFRQAVARFMEKVRGDRVTFDPNRIVMSGGA 130

Query: 1276 TGAHETMAFCLADPGDAFLVPTPYYPGFDRDLRWRTGVKLLPVVCESSNNFMITREALDA 1097
            TGAHET+AFCLA+PGDAFLVPTPYYPGFDRDLRWRTGV+L PVVC+SSN+F +T++AL+A
Sbjct: 131  TGAHETLAFCLANPGDAFLVPTPYYPGFDRDLRWRTGVQLFPVVCDSSNDFKVTKKALEA 190

Query: 1096 AYQKAQDSNIKVKGLLITNPSNPLGTILDRDTLQSLMDFINEKNIHLICDEIYAATVFTK 917
            AY+KAQ+SNI +KGLLI NPSNPLGT+LD DTL+ ++ FIN KNIHLICDEIYAATVF +
Sbjct: 191  AYEKAQESNINIKGLLINNPSNPLGTLLDSDTLRDIVTFINSKNIHLICDEIYAATVFDQ 250

Query: 916  PMYVSIAEILEDDKKCNRDLIHLVYSLSKDMGFPGFRIGIIYSYNDKVVNCARKMSSFGL 737
            P + S++EI+E+   CN+DLIH+VYSLSKD+GFPGFR+GI+YSYND VVN ARKMSSFGL
Sbjct: 251  PKFFSVSEIVEEHVGCNKDLIHIVYSLSKDLGFPGFRVGIVYSYNDTVVNIARKMSSFGL 310

Query: 736  VSTQTQELIANMLSDENFVDKYTSESRKRLAARHGVFTRGLMQVRVGNLQSNAGLFFWMD 557
            VSTQTQ LIA+MLSDE F++K+ +ES +RL  RHG+FT+GL QV +  L+SNAGLFFWMD
Sbjct: 311  VSTQTQHLIASMLSDEIFIEKFIAESSERLGKRHGMFTKGLAQVGISTLKSNAGLFFWMD 370

Query: 556  LRRLLKEPTIAAEMELWREIINEVKINVSPGSSFHCSEPGWFRVCYANMDDETMRIALRR 377
            LRRLLKEPT+ +E+ELWR IINEVK+NVSPG SFHCSEPGWFRVC+ANMDDETMR ALRR
Sbjct: 371  LRRLLKEPTLESELELWRIIINEVKLNVSPGCSFHCSEPGWFRVCFANMDDETMRTALRR 430

Query: 376  IKKFVLQAD--EREAAAKKQMLNKRNLQLSLSFRRWDDTIMNTP 251
            I+ FVLQ      +AA KKQ  ++  LQ+SLSFRR DD  MN+P
Sbjct: 431  IRNFVLQTKGLNNKAAVKKQ-CSRSKLQISLSFRRMDD-FMNSP 472


>emb|CAA67118.1| ACC synthase [Nicotiana tabacum]
          Length = 483

 Score =  728 bits (1878), Expect = 0.0
 Identities = 352/489 (71%), Positives = 410/489 (83%), Gaps = 1/489 (0%)
 Frame = -2

Query: 1630 LLSRVATNDGHGENSPYFDGWKAYDSDPFHSDENPQGVIQMGLAENQLCFDLIQEWILKN 1451
            LLS+VATN+GHGENS YFDGWKAY+ DPFH  +N  GVIQMGLAENQLCFDLIQEW++KN
Sbjct: 11   LLSKVATNNGHGENSAYFDGWKAYEIDPFHPTQNSDGVIQMGLAENQLCFDLIQEWVVKN 70

Query: 1450 PKASICTSEGVNNFKDAAIFQDYHGLPQFREAIAKFMAKVRGNTVTFDPDRVVMSGGATG 1271
            PKASICT+EG  +FKD AI+QDYHGLP+FR A+A+FM KVRG+ +TF+P+R+VMSGGATG
Sbjct: 71   PKASICTAEGSQDFKDIAIYQDYHGLPEFRSAVARFMGKVRGDRITFEPERIVMSGGATG 130

Query: 1270 AHETMAFCLADPGDAFLVPTPYYPGFDRDLRWRTGVKLLPVVCESSNNFMITREALDAAY 1091
            AHE +AFCLADPG+AFLVPTPYYPGFDRDLRWRTGV+L PVVCESSNNF +T+EAL+AAY
Sbjct: 131  AHELLAFCLADPGEAFLVPTPYYPGFDRDLRWRTGVQLFPVVCESSNNFKVTKEALEAAY 190

Query: 1090 QKAQDSNIKVKGLLITNPSNPLGTILDRDTLQSLMDFINEKNIHLICDEIYAATVFTKPM 911
            QKAQ+SNI VKGLL+ NPSNPLGTILDR+TL+  + FINEKNIHL+CDEIYAAT+F KP 
Sbjct: 191  QKAQESNITVKGLLLNNPSNPLGTILDRETLKDTVRFINEKNIHLVCDEIYAATIFNKPD 250

Query: 910  YVSIAE-ILEDDKKCNRDLIHLVYSLSKDMGFPGFRIGIIYSYNDKVVNCARKMSSFGLV 734
            ++SI+E I+E+D +C+RDLIH+VYSLSKD+GFPGFR+GIIYSYND V NCARKMSSFGLV
Sbjct: 251  FISISEVIMEEDVECDRDLIHIVYSLSKDLGFPGFRVGIIYSYNDVVTNCARKMSSFGLV 310

Query: 733  STQTQELIANMLSDENFVDKYTSESRKRLAARHGVFTRGLMQVRVGNLQSNAGLFFWMDL 554
            STQTQ LI+NMLSDENFV K+  ES +RL  RHG+FTRGL QV +  L+SNAGLF WMDL
Sbjct: 311  STQTQFLISNMLSDENFVTKFIGESSERLQKRHGMFTRGLAQVGINTLKSNAGLFIWMDL 370

Query: 553  RRLLKEPTIAAEMELWREIINEVKINVSPGSSFHCSEPGWFRVCYANMDDETMRIALRRI 374
            RRLLKE T  AE+ELWR IINEVK+NVSPG SFHCSEPGWFRVC+ANMDDETMRIALRRI
Sbjct: 371  RRLLKELTFEAELELWRIIINEVKLNVSPGCSFHCSEPGWFRVCFANMDDETMRIALRRI 430

Query: 373  KKFVLQADEREAAAKKQMLNKRNLQLSLSFRRWDDTIMNTPRMVTXXXXXXXXXXXXXXP 194
              FV+Q    E   KK    +  L++SLSFR+ DD  MN+P                  P
Sbjct: 431  NNFVIQRKGIEGGVKKLQCRRSKLEISLSFRKLDD-FMNSPH----------------SP 473

Query: 193  LTSPLVRAR 167
            ++SPLV+AR
Sbjct: 474  MSSPLVQAR 482


>gb|AAR99392.1| ACC synthase ACS3a [Nicotiana attenuata]
          Length = 484

 Score =  721 bits (1862), Expect = 0.0
 Identities = 340/464 (73%), Positives = 406/464 (87%), Gaps = 2/464 (0%)
 Frame = -2

Query: 1636 KNLLSRVATNDGHGENSPYFDGWKAYDSDPFHSDENPQGVIQMGLAENQLCFDLIQEWIL 1457
            + LLS++ATNDGHGENS YFDGWKAY++DPFH   NP GVIQMGLAENQLCFDLIQEW++
Sbjct: 11   QQLLSKIATNDGHGENSAYFDGWKAYENDPFHLTNNPNGVIQMGLAENQLCFDLIQEWVV 70

Query: 1456 KNPKASICTSEGVNNFKDAAIFQDYHGLPQFREAIAKFMAKVRGNTVTFDPDRVVMSGGA 1277
             NPKASICT+EG  +F+D AIFQDYHGLP+FR+A+A+FM KVRG+ VTFDP+R+VMSGGA
Sbjct: 71   NNPKASICTTEGAEDFRDIAIFQDYHGLPEFRQAVARFMEKVRGDKVTFDPNRIVMSGGA 130

Query: 1276 TGAHETMAFCLADPGDAFLVPTPYYPGFDRDLRWRTGVKLLPVVCESSNNFMITREALDA 1097
            TGAHET+AFCLA+PGDAFLVPTPYYPGFDRDLRWRTGV+L PVVC+SSN+F +T++AL++
Sbjct: 131  TGAHETLAFCLANPGDAFLVPTPYYPGFDRDLRWRTGVQLFPVVCDSSNDFKVTKKALES 190

Query: 1096 AYQKAQDSNIKVKGLLITNPSNPLGTILDRDTLQSLMDFINEKNIHLICDEIYAATVFTK 917
            AY+KAQ+SNI +KGLLI NPSNPLGTILD DTL+ ++ FIN KNIHLICDEIYAATVF +
Sbjct: 191  AYEKAQESNINIKGLLINNPSNPLGTILDSDTLKDIVTFINSKNIHLICDEIYAATVFDQ 250

Query: 916  PMYVSIAEILEDDKKCNRDLIHLVYSLSKDMGFPGFRIGIIYSYNDKVVNCARKMSSFGL 737
            P ++S++EI+E+   CN+DLIH+VYSLSKD+GFPGFR+GI+YSYND VVN ARKMSSFGL
Sbjct: 251  PKFISVSEIVEEHVGCNKDLIHIVYSLSKDLGFPGFRVGIVYSYNDTVVNIARKMSSFGL 310

Query: 736  VSTQTQELIANMLSDENFVDKYTSESRKRLAARHGVFTRGLMQVRVGNLQSNAGLFFWMD 557
            VSTQTQ LIA+MLSDE F++K+ +ES +RL  RH +FT+GL QV +  L+SNAGLFFWMD
Sbjct: 311  VSTQTQHLIASMLSDETFIEKFIAESSERLGKRHEMFTKGLAQVGISTLKSNAGLFFWMD 370

Query: 556  LRRLLKEPTIAAEMELWREIINEVKINVSPGSSFHCSEPGWFRVCYANMDDETMRIALRR 377
            LRRLLKE T  +E+ELWR IINEVK+NVSPG SFHCSEPGWFRVC+ANMDDETMRIALRR
Sbjct: 371  LRRLLKELTFESELELWRIIINEVKLNVSPGCSFHCSEPGWFRVCFANMDDETMRIALRR 430

Query: 376  IKKFVLQAD--EREAAAKKQMLNKRNLQLSLSFRRWDDTIMNTP 251
            I+ FVLQ      ++A KKQ  ++  LQ+SLSFRR DD  MN+P
Sbjct: 431  IRNFVLQTKGLNSKSAVKKQ-CSRSKLQISLSFRRMDD-FMNSP 472


>gb|AAF97614.1|U18056_1 1-amino-cyclopropane-1-carboxylate synthase [Solanum lycopersicum]
          Length = 485

 Score =  721 bits (1861), Expect = 0.0
 Identities = 338/463 (73%), Positives = 401/463 (86%), Gaps = 1/463 (0%)
 Frame = -2

Query: 1636 KNLLSRVATNDGHGENSPYFDGWKAYDSDPFHSDENPQGVIQMGLAENQLCFDLIQEWIL 1457
            + LLS++ATNDGHGENSPYFDGWKAY ++PFH  +NP GVIQMGLAENQLCFDLIQEW++
Sbjct: 11   QQLLSKIATNDGHGENSPYFDGWKAYANNPFHLTDNPTGVIQMGLAENQLCFDLIQEWVV 70

Query: 1456 KNPKASICTSEGVNNFKDAAIFQDYHGLPQFREAIAKFMAKVRGNTVTFDPDRVVMSGGA 1277
             NPKASICT EG  NF+D AIFQDYHGLP+FR+A+A+FM KVRG+ VTFDP+R+VMSGGA
Sbjct: 71   NNPKASICTVEGAENFQDIAIFQDYHGLPEFRQAVARFMEKVRGDRVTFDPNRIVMSGGA 130

Query: 1276 TGAHETMAFCLADPGDAFLVPTPYYPGFDRDLRWRTGVKLLPVVCESSNNFMITREALDA 1097
            TGAHE +AFCLADPGDAFLVPTPYYPGFDRDLRWRTGV+L PVVCES N+F +T +AL+ 
Sbjct: 131  TGAHEMLAFCLADPGDAFLVPTPYYPGFDRDLRWRTGVQLFPVVCESCNDFKVTTKALEE 190

Query: 1096 AYQKAQDSNIKVKGLLITNPSNPLGTILDRDTLQSLMDFINEKNIHLICDEIYAATVFTK 917
            AY+KAQ SNIK+KGLLI NPSNPLGT+LD+DTL+ ++ FIN KNIHL+CDEIYAATVF +
Sbjct: 191  AYEKAQQSNIKIKGLLINNPSNPLGTLLDKDTLRDIVTFINSKNIHLVCDEIYAATVFDQ 250

Query: 916  PMYVSIAEILEDDKKCNRDLIHLVYSLSKDMGFPGFRIGIIYSYNDKVVNCARKMSSFGL 737
            P ++S++EI+ED  +CN+DLIH+VYSLSKD+GFPGFR+GI+YSYND VVN ARKMSSFGL
Sbjct: 251  PRFISVSEIVEDMIECNKDLIHIVYSLSKDLGFPGFRVGIVYSYNDTVVNIARKMSSFGL 310

Query: 736  VSTQTQELIANMLSDENFVDKYTSESRKRLAARHGVFTRGLMQVRVGNLQSNAGLFFWMD 557
            VSTQTQ L+A+MLSDE F+DK+ +ES +RL  R G+FT+GL +V +  L+SNAGLFFWMD
Sbjct: 311  VSTQTQHLLASMLSDEVFIDKFIAESSERLGERQGMFTKGLAEVGISTLKSNAGLFFWMD 370

Query: 556  LRRLLKEPTIAAEMELWREIINEVKINVSPGSSFHCSEPGWFRVCYANMDDETMRIALRR 377
            LRRLLKE T  +E+ELWR IINEVK+NVSPG SFHCSEPGWFRVC+ANMDDETMRIAL+R
Sbjct: 371  LRRLLKEATFDSELELWRIIINEVKLNVSPGCSFHCSEPGWFRVCFANMDDETMRIALKR 430

Query: 376  IKKFVLQADE-REAAAKKQMLNKRNLQLSLSFRRWDDTIMNTP 251
            I  FVLQ       AA K+  ++R LQ+SLSFRR D   MN+P
Sbjct: 431  ISYFVLQPKGLNNIAAIKKQCSRRKLQISLSFRRLDHEFMNSP 473